##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9397010
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17537163416874	33.0	31.0	34.0	30.0	34.0
2	32.31770084314053	34.0	31.0	34.0	30.0	34.0
3	33.0360640246206	34.0	31.0	34.0	30.0	37.0
4	35.864328653475944	37.0	35.0	37.0	35.0	37.0
5	35.76904068421764	37.0	35.0	37.0	35.0	37.0
6	35.77558680899563	37.0	35.0	37.0	33.0	37.0
7	35.76347880868489	37.0	35.0	37.0	33.0	37.0
8	36.026110965083575	37.0	35.0	37.0	33.0	39.0
9	37.29989528584092	39.0	37.0	39.0	34.0	39.0
10-11	37.23475600217516	39.0	37.0	39.0	33.0	39.0
12-13	37.362025261226705	39.0	37.0	39.0	33.0	39.5
14-15	38.413619385315116	40.0	38.0	41.0	33.0	41.0
16-17	38.38742993782065	40.0	38.0	41.0	33.0	41.0
18-19	38.41853132006883	40.0	38.0	41.0	33.5	41.0
20-21	38.38453752486142	40.0	38.0	41.0	34.0	41.0
22-23	38.31860527541481	40.0	38.0	41.0	34.0	41.0
24-25	38.219057403672764	40.0	38.0	41.0	33.0	41.0
26-27	38.0730057804616	40.0	37.5	41.0	33.0	41.0
28-29	37.97492376938379	40.0	37.0	41.0	33.0	41.0
30-31	37.88586514538646	40.0	37.0	41.0	33.0	41.0
32-33	38.04430799206936	40.0	37.0	41.0	33.0	41.0
34-35	38.030228209984	40.0	37.0	41.0	33.0	41.0
36-37	37.93466841655109	40.0	37.0	41.0	33.0	41.0
38-39	37.820659725489705	40.0	37.0	41.0	33.0	41.0
40-41	37.773497857348616	40.0	37.0	41.0	33.0	41.0
42-43	37.70074461917445	40.0	37.0	41.0	32.5	41.0
44-45	37.71984767750918	40.0	37.0	41.0	32.5	41.0
46-47	37.73876203457561	40.0	37.0	41.0	32.5	41.0
48-49	37.66769530064166	40.0	37.0	41.0	32.0	41.0
50-51	37.58400323409427	40.0	36.5	41.0	32.0	41.0
52-53	37.453899643526	39.5	36.0	41.0	32.0	41.0
54-55	37.32481950698981	39.0	36.0	41.0	31.0	41.0
56-57	37.15890719000018	39.0	35.5	41.0	31.0	41.0
58-59	36.999671222696186	39.0	35.0	40.5	31.0	41.0
60-61	36.831381134434494	39.0	35.0	40.0	31.0	41.0
62-63	36.60781869652353	38.0	35.0	40.0	31.0	41.0
64-65	36.384827981074935	38.0	35.0	40.0	31.0	41.0
66-67	36.124566436195465	37.0	34.5	40.0	30.0	41.0
68-69	35.834200057175536	37.0	34.0	39.5	30.0	41.0
70-71	35.54378509144149	36.0	34.0	39.0	30.0	41.0
72-73	35.218196350118305	36.0	34.0	39.0	29.5	40.0
74-75	34.770469748602466	35.5	33.5	38.5	29.0	40.0
76-77	33.91497735562314	35.0	32.5	37.0	28.5	39.0
78-79	34.205129622955866	35.0	33.0	37.0	29.0	39.0
80-81	34.07516712715389	35.0	33.0	36.5	29.0	39.0
82-83	33.83008303733116	35.0	33.0	36.0	29.0	37.5
84-85	33.628659668682076	35.0	33.0	36.0	29.0	37.0
86-87	33.44016179573185	35.0	33.0	35.0	29.0	37.0
88-89	33.25416265207578	35.0	33.0	35.0	29.0	36.0
90-91	33.14456260514737	35.0	33.0	35.0	29.0	36.0
92-93	33.088250408166346	35.0	33.0	35.0	29.0	36.0
94-95	33.05867764071722	34.0	33.0	35.0	29.0	35.5
96-97	33.03095552768651	34.0	33.0	35.0	29.0	35.0
98-99	33.10303104111043	34.0	33.0	35.0	29.0	35.0
100-101	32.451885046989844	34.0	31.5	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	3.0
19	15.0
20	88.0
21	420.0
22	2005.0
23	6469.0
24	16778.0
25	34508.0
26	60884.0
27	93586.0
28	131084.0
29	171850.0
30	216253.0
31	266559.0
32	327663.0
33	412108.0
34	544107.0
35	744839.0
36	1128019.0
37	1867102.0
38	2228530.0
39	1085642.0
40	58496.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.056478260360606	0.009609562312637479	26.900815056896484	31.03309712043027
2	14.16608942851241	25.964170404747083	37.349130130746175	22.52061003599433
3	15.663109861541063	25.686447071994177	34.1323676360885	24.518075430376257
4	12.974861152643236	24.946860756772633	37.15904314244637	24.91923494813776
5	16.95537197470259	36.996736195875066	32.58479026839388	13.463101561028454
6	27.063087088339806	38.09548994839848	20.910821633689867	13.930601329571854
7	24.96407899959668	33.036710613269534	23.668922348704534	18.330288038429245
8	23.696973824652733	32.52468604375222	22.66816785339166	21.110172278203386
9	23.148160957581187	20.587633726046903	24.500069703022557	31.764135613349353
10-11	24.402368412931345	26.26922286982774	29.241769456454765	20.086639260786143
12-13	24.503554854150416	24.368522540680495	28.953528835235886	22.1743937699332
14-15	22.039462552450196	26.821100541555236	26.672728878653956	24.466708027340612
16-17	21.47808642921236	28.885869842826565	27.69987532201739	21.93616840594368
18-19	21.58762725874546	28.167726807029624	29.034109214721056	21.21053671950386
20-21	22.27090875567405	27.631332982312095	28.724970780009457	21.372787482004398
22-23	22.155226292736426	27.410821026423527	28.50296702679137	21.93098565404868
24-25	21.900359380936873	27.68648082735651	28.68653970037784	21.726620091328773
26-27	21.868324905184995	27.90380753838866	28.526131609057824	21.70173594736852
28-29	21.845069797363763	27.71294213260809	28.517399691783574	21.92458837824457
30-31	21.995023106699684	27.61130946787434	28.65674201591127	21.73692540951471
32-33	21.85762010698529	27.950832565560326	28.442961688394377	21.74858563906001
34-35	21.985723139388668	27.77945428074389	28.47348892163857	21.761333658228867
36-37	21.97621262150598	27.82508399308128	28.469713644522347	21.7289897408904
38-39	21.797824868048938	27.950089762738894	28.555393876112735	21.696691493099436
40-41	22.056393810438383	27.662963204062308	28.322804776933154	21.95783820856616
42-43	21.915787083698824	27.77384586894009	28.344287534892963	21.966079512468127
44-45	21.998695440688067	28.04855015768079	28.0339073729381	21.918847028693044
46-47	22.170461760438943	27.77859906368137	27.837816349730616	22.21312282614907
48-49	21.96940373501503	27.941740318474917	27.967826572645794	22.121029373864257
50-51	22.00295235384289	28.174793692412425	27.745766621750406	22.076487331994283
52-53	22.190737435424435	27.856881623280284	27.769954434229476	22.182426507065802
54-55	22.03421974685782	27.877610627535592	27.880637280239302	22.207532345367284
56-57	22.145759042357888	28.18552177069681	27.574512783890054	22.094206403055253
58-59	22.18493523689201	27.953608341871593	27.737756346772567	22.12370007446383
60-61	22.152725402675717	28.06717660885428	27.715017443605166	22.06508054486484
62-63	22.10740944494666	28.27794656589141	27.59596272347531	22.01868126568662
64-65	22.19640679764958	27.990629431505266	27.566857036099968	22.24610673474519
66-67	22.10463565036233	28.059508119314934	27.617607244702818	22.218248985619915
68-69	22.144682154355355	28.09563625807349	27.617589901172057	22.142091686399095
70-71	22.215669958706563	27.71365838087411	27.675596975139527	22.39507468527979
72-73	22.111591622148733	27.904363083706762	27.651729513596724	22.33231578054778
74-75	22.039458848790826	28.051463550514576	27.65203757760462	22.257040023089978
76-77	22.29225419942792	27.774901476368402	27.51805366690317	22.41479065730051
78-79	22.072969752528696	27.881841037087128	27.675876332295868	22.36931287808831
80-81	22.120399364319823	28.011308155479924	27.54461147020103	22.323681009999223
82-83	22.225907444085465	27.75894413447479	27.65284597005663	22.362302451383112
84-85	22.181634884763568	27.800801180997105	27.74498368316513	22.2725802510742
86-87	22.126746397330376	27.902327822598593	27.706000685338815	22.264925094732217
88-89	22.17373092539454	27.748313226234643	27.655086624567836	22.422869223802973
90-91	22.192081040889384	27.71627547561173	27.78158232789472	22.310061155604156
92-93	22.15574206045623	27.7822264263291	27.767249923685412	22.29478158952926
94-95	22.124253244193714	27.62546747346097	27.84270972133294	22.407569561012377
96-97	22.002427213232103	27.79029572970073	27.937650960100314	22.269626096966853
98-99	22.44261237277815	28.914193476506895	27.983656765827646	20.659537384887305
100-101	26.551623540380735	35.79181950406886	28.749143465811443	8.90741348973896
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1631.0
1	1504.5
2	2188.0
3	3184.0
4	4184.5
5	6504.0
6	8375.5
7	9634.5
8	10467.5
9	10311.0
10	9714.5
11	9114.5
12	10131.0
13	13261.0
14	20399.0
15	31179.5
16	40716.5
17	46135.0
18	47234.0
19	45564.0
20	42984.5
21	40675.0
22	40606.0
23	44883.0
24	53885.5
25	67705.0
26	85536.0
27	104676.5
28	126270.0
29	150357.0
30	173977.5
31	197168.5
32	220206.0
33	242986.0
34	265495.0
35	286563.0
36	308001.5
37	326600.5
38	342338.0
39	356392.5
40	368313.0
41	379790.0
42	387313.0
43	394444.5
44	400580.5
45	404782.0
46	408250.5
47	408609.0
48	404757.5
49	396485.0
50	386890.0
51	374297.5
52	355364.0
53	333590.5
54	312451.5
55	289197.0
56	263960.5
57	237794.5
58	210939.0
59	182525.5
60	152566.5
61	124610.0
62	99510.5
63	77409.5
64	57913.5
65	42931.0
66	32235.0
67	24223.5
68	18345.0
69	13367.0
70	9553.5
71	6941.0
72	4921.0
73	3384.5
74	2295.0
75	1540.0
76	1059.5
77	708.0
78	476.0
79	324.5
80	223.0
81	157.5
82	125.0
83	100.5
84	73.5
85	49.0
86	32.5
87	28.0
88	22.0
89	14.5
90	9.0
91	5.5
92	4.5
93	3.5
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0012770019399787804
2	0.0026923457567885954
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	2.1283365666313007E-5
18-19	6.651051770722815E-4
20-21	0.0017932092389971184
22-23	0.002032897821340212
24-25	8.676509234866558E-4
26-27	0.007173019732673749
28-29	1.65186084255347E-4
30-31	1.6008703611979762E-5
32-33	4.2802658130675237E-5
34-35	0.0015732629069978787
36-37	0.03589947262325112
38-39	0.016536372022242726
40-41	0.021471307649241076
42-43	0.002001703659938196
44-45	0.0052883233541126656
46-47	0.004082421089143646
48-49	0.0020948478615313553
50-51	0.002081352877063883
52-53	3.3173763650203497E-4
54-55	0.0038081480852449243
56-57	0.014494664894040436
58-59	0.007725138375189546
60-61	0.003183670250503205
62-63	0.0015665257407823782
64-65	0.0024470502424132437
66-67	2.702827226420928E-4
68-69	4.2007412016907596E-4
70-71	2.1263309946054984E-4
72-73	3.9136630281341495E-5
74-75	9.046266038138266E-4
76-77	0.004673632049963333
78-79	1.964597146483615E-4
80-81	6.869242930432772E-5
82-83	5.155104197543593E-4
84-85	0.002253275195536961
86-87	0.01820883534827246
88-89	0.06410041816644275
90-91	0.00256069722012634
92-93	0.0014848149035017288
94-95	0.0026610428934734543
96-97	0.01376969439573438
98-99	3.365990169962308E-5
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	248.0
20-21	928.0
22-23	1780.0
24-25	3567.0
26-27	5174.0
28-29	10230.0
30-31	22930.0
32-33	30576.0
34-35	44154.0
36-37	67250.0
38-39	72945.0
40-41	74692.0
42-43	85174.0
44-45	97473.0
46-47	97565.0
48-49	89339.0
50-51	81979.0
52-53	81227.0
54-55	89354.0
56-57	93522.0
58-59	93728.0
60-61	91921.0
62-63	88086.0
64-65	92756.0
66-67	100676.0
68-69	95873.0
70-71	95263.0
72-73	92841.0
74-75	94355.0
76-77	95738.0
78-79	101304.0
80-81	102105.0
82-83	99019.0
84-85	100065.0
86-87	105085.0
88-89	112219.0
90-91	121597.0
92-93	126370.0
94-95	159540.0
96-97	262399.0
98-99	1126457.0
100-101	4989506.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.80743122881871
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.31036802555874	77.49300693602441
2	6.794390794352783	11.660184416562908
3	1.6385517074919864	4.217997388664464
4	0.5774501772158592	1.981980654780761
5	0.25934014525472215	1.1126655839408215
6	0.13014186342815925	0.6700283397661255
7	0.08411958511784542	0.5052659860497398
8	0.05486906489628114	0.37665388101417885
9	0.03293025110495908	0.2543094231332857
>10	0.11504310214277137	1.5382884015059153
>50	0.0024239055743662708	0.1342902517086991
>100	3.7137786150724623E-4	0.05532873684868897
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0641682833156504E-5
2	0.0	9.577514549840854E-5	0.0	0.0	1.0641682833156504E-5
3	0.0	9.577514549840854E-5	0.0	0.0	1.0641682833156504E-5
4	1.0641682833156504E-5	9.577514549840854E-5	0.0	0.0	1.0641682833156504E-5
5	1.0641682833156504E-5	1.2770019399787805E-4	0.0	0.0	1.0641682833156504E-5
6	1.0641682833156504E-5	1.2770019399787805E-4	0.0	0.0	1.0641682833156504E-5
7	1.0641682833156504E-5	1.2770019399787805E-4	0.0	0.0	1.0641682833156504E-5
8	2.1283365666313007E-5	1.3834187683103456E-4	0.0	0.0	1.0641682833156504E-5
9	2.1283365666313007E-5	2.4475870516259957E-4	0.0	0.0	1.0641682833156504E-5
10-11	3.192504849946951E-5	2.4475870516259957E-4	0.0	0.0	1.0641682833156504E-5
12-13	3.192504849946951E-5	2.6072122941233434E-4	0.0	0.0	1.0641682833156504E-5
14-15	3.192504849946951E-5	3.884214234102124E-4	0.0	1.0641682833156504E-5	1.5962524249734756E-5
16-17	3.192504849946951E-5	4.2566731332626014E-4	0.0	1.0641682833156504E-5	2.1283365666313007E-5
18-19	4.2566731332626015E-5	4.2566731332626014E-4	0.0	2.1283365666313007E-5	2.660420708289126E-5
20-21	4.2566731332626015E-5	4.3098815474283843E-4	0.0	4.788757274920427E-5	3.192504849946951E-5
22-23	4.2566731332626015E-5	4.6823404465888617E-4	0.0	6.917093841551728E-5	3.192504849946951E-5
24-25	4.788757274920426E-5	5.161216174080905E-4	0.0	8.513346266525204E-5	3.192504849946951E-5
26-27	6.385009699893903E-5	5.533675073241382E-4	0.0	1.5962524249734755E-4	3.192504849946951E-5
28-29	8.513346266525203E-5	5.746508729904512E-4	0.0	4.522715204091514E-4	3.192504849946951E-5
30-31	8.513346266525203E-5	6.278592871562337E-4	0.0	0.0016920275704718842	3.192504849946951E-5
32-33	9.577514549840854E-5	6.278592871562337E-4	0.0	0.004166218829180771	3.192504849946951E-5
34-35	9.577514549840854E-5	7.129927498214858E-4	0.0	0.007747145102537935	3.192504849946951E-5
36-37	1.0641682833156503E-4	7.981262124867378E-4	0.0	0.013307424382862209	3.192504849946951E-5
38-39	1.1705851116472154E-4	8.619763094856769E-4	0.0	0.02101200275406752	3.192504849946951E-5
40-41	1.1705851116472154E-4	9.630722964006637E-4	0.0	0.028376047274611817	3.192504849946951E-5
42-43	1.1705851116472154E-4	0.001074809966148807	0.0	0.03589971703765347	3.192504849946951E-5
44-45	1.2770019399787805E-4	0.001159943428814059	0.0	0.044578009388092595	3.192504849946951E-5
46-47	1.2770019399787805E-4	0.001159943428814059	0.0	0.05384691513577192	3.192504849946951E-5
48-49	1.2770019399787805E-4	0.001159943428814059	0.0	0.06296683732378704	4.2566731332626015E-5
50-51	1.3834187683103456E-4	0.0012503977328958893	0.0	0.07225170559571609	4.2566731332626015E-5
52-53	1.5962524249734755E-4	0.0012557185743124674	0.0	0.08113218991998519	4.2566731332626015E-5
54-55	1.7026692533050406E-4	0.001266360257145624	0.0	0.09039577482624793	4.2566731332626015E-5
56-57	1.7026692533050406E-4	0.0012770019399787804	0.0	0.09993072264475615	4.2566731332626015E-5
58-59	1.7026692533050406E-4	0.0012823227813953588	0.0	0.10834829376578295	4.2566731332626015E-5
60-61	1.7026692533050406E-4	0.0013621354026440325	0.0	0.11687760255655788	4.2566731332626015E-5
62-63	1.862294495802388E-4	0.0013993812925600802	0.0	0.12510362338658787	4.2566731332626015E-5
64-65	1.9155029099681708E-4	0.0015324023279745366	0.0	0.13311148971853815	4.788757274920426E-5
66-67	1.9155029099681708E-4	0.0015324023279745366	0.0	0.14109807268482208	5.852925558236077E-5
68-69	2.0219197382997359E-4	0.001606894107806632	0.0	0.14910061817535578	6.385009699893903E-5
70-71	2.0219197382997359E-4	0.0016601025219724146	0.0	0.15655511699998192	6.385009699893903E-5
72-73	2.0219197382997359E-4	0.0016813858876387278	0.0	0.162988014272625	6.385009699893903E-5
74-75	2.0219197382997359E-4	0.0017133109361381971	0.0	0.1685482935529493	6.385009699893903E-5
76-77	2.0219197382997359E-4	0.0017505568260542449	0.0	0.17376803898261256	6.385009699893903E-5
78-79	2.1283365666313007E-4	0.001755877667470823	0.0	0.1781311289442067	6.385009699893903E-5
80-81	2.1283365666313007E-4	0.0017611985088874012	0.0	0.18047762000891773	7.449177983209553E-5
82-83	2.1283365666313007E-4	0.0017665193503039796	0.0	0.18196213476414308	8.513346266525203E-5
84-85	2.2347533949628658E-4	0.001771840191720558	0.0	0.18320721165562237	9.045430408183028E-5
86-87	2.2347533949628658E-4	0.0018197277644697622	0.0	0.18341472447086893	1.0109598691498679E-4
88-89	2.2347533949628658E-4	0.0018835778614687013	0.0	0.18348389540928445	1.0641682833156503E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10490	0.0	45.61345	1
GTATCAA	17465	0.0	32.094074	1
ATCAACG	20215	0.0	26.964714	3
TCAACGC	21195	0.0	25.71794	4
CAACGCA	21505	0.0	25.327843	5
AACGCAG	21835	0.0	25.002268	6
ACGCAGA	22435	0.0	24.370731	7
TATCAAC	22955	0.0	24.055386	2
CGCAGAG	26345	0.0	20.753742	8
GTACATG	25235	0.0	19.901802	1
TACATGG	25710	0.0	19.031887	2
ACATGGG	25060	0.0	19.009682	3
GCAGAGT	31620	0.0	17.212488	9
GAGTACT	17085	0.0	17.122494	12-13
AGAGTAC	28080	0.0	17.054173	10-11
CTTATAC	6775	0.0	16.964779	1
CAGAGTA	30820	0.0	16.375702	10-11
CATGGGG	19430	0.0	16.202383	4
GTACTTT	19495	0.0	14.985464	14-15
AGTACTT	18910	0.0	14.787344	12-13
>>END_MODULE