FastQCFastQC Report
Fri 27 May 2016
SRR2139802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9853745
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA116400.050.4681131
TATCTCG117850.037.70033694-95
GTATCAA212600.036.603481
CCGTCTT43950.031.99253392-93
TATGCCG43250.030.20556388-89
TGCCGTC45250.029.55404390-91
TCAACGC262700.028.9293164
ATCAACG263800.028.7766233
GCCGTCT46150.028.57240990-91
CAACGCA267550.028.3574815
ATGCCGT45600.028.0506888-89
AACGCAG274300.027.705916
TATCAAC279700.027.474532
CGTATGC53700.025.0477286-87
TCGTATG53050.024.70322484-85
CTCGTAT54350.024.53911284-85
CTATCTC200600.024.3184394-95
ATCTCGT94600.024.31601794-95
ACGCAGA318800.023.851827
CGCAGAG323050.023.4987568
Produced by FastQC (version 0.11.5)