##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9853745
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861887840612884	31.0	31.0	34.0	30.0	34.0
2	32.02343261369155	31.0	31.0	34.0	30.0	34.0
3	32.0483745012683	33.0	31.0	34.0	30.0	34.0
4	35.75599642572443	37.0	35.0	37.0	35.0	37.0
5	35.569310957407566	37.0	35.0	37.0	33.0	37.0
6	35.573923213965855	37.0	35.0	37.0	33.0	37.0
7	35.46827201231613	37.0	35.0	37.0	33.0	37.0
8	35.44977721668259	37.0	35.0	37.0	33.0	37.0
9	36.99233306727544	39.0	37.0	39.0	33.0	39.0
10-11	36.88756335789083	39.0	37.0	39.0	33.0	39.0
12-13	36.829925373550864	39.0	37.0	39.0	32.0	39.0
14-15	37.95349742661293	40.0	37.0	41.0	33.0	41.0
16-17	37.9356414743836	40.0	37.0	41.0	33.0	41.0
18-19	37.97571354850364	40.0	37.0	41.0	33.0	41.0
20-21	37.955680466252595	40.0	37.0	41.0	33.0	41.0
22-23	37.88822185578166	40.0	37.0	41.0	32.5	41.0
24-25	37.801004002595164	40.0	37.0	41.0	32.0	41.0
26-27	37.612615747868034	39.0	37.0	41.0	32.0	41.0
28-29	37.50773820859001	39.0	36.0	41.0	32.0	41.0
30-31	37.35912395456453	39.0	36.0	41.0	31.5	41.0
32-33	37.52808163799827	39.0	36.0	41.0	32.0	41.0
34-35	37.488864752499715	39.0	36.0	41.0	32.0	41.0
36-37	37.402976983960926	39.0	36.0	41.0	31.5	41.0
38-39	37.30011201673166	39.0	36.0	41.0	31.0	41.0
40-41	37.21317551977165	39.0	36.0	41.0	31.0	41.0
42-43	37.16967399500011	39.0	36.0	40.5	31.0	41.0
44-45	37.19761272111768	39.0	36.0	40.5	31.0	41.0
46-47	37.304151764157716	39.0	36.0	41.0	31.0	41.0
48-49	37.25121799489959	39.0	36.0	41.0	31.0	41.0
50-51	37.14469930409259	39.0	36.0	41.0	31.0	41.0
52-53	37.056229448132086	39.0	35.5	41.0	31.0	41.0
54-55	36.94568937210394	39.0	35.0	40.0	31.0	41.0
56-57	36.832091692284074	39.0	35.0	40.0	31.0	41.0
58-59	36.70592940959165	39.0	35.0	40.0	30.0	41.0
60-61	36.50334357272156	38.0	35.0	40.0	30.0	41.0
62-63	36.30413453167328	38.0	34.5	40.0	30.0	41.0
64-65	36.13673129571927	38.0	34.0	40.0	29.0	41.0
66-67	35.895779021428766	37.5	34.0	40.0	29.0	41.0
68-69	35.62859206052424	37.0	34.0	39.5	29.0	41.0
70-71	35.337368840537266	36.5	34.0	39.0	29.0	41.0
72-73	35.03819997948992	36.0	34.0	39.0	29.0	40.0
74-75	34.67086875685019	35.5	33.0	38.5	28.0	40.0
76-77	33.57561650847158	35.0	31.5	37.0	27.0	39.0
78-79	33.94125308824765	35.0	32.5	37.0	28.0	39.0
80-81	33.79688379228102	35.0	33.0	37.0	28.0	39.0
82-83	33.53887694686322	35.0	32.5	36.0	28.0	38.5
84-85	33.31625379856959	35.0	32.0	36.0	28.0	37.0
86-87	33.10312200206247	35.0	32.0	35.0	28.0	37.0
88-89	32.87907292101606	34.0	32.0	35.0	27.0	36.5
90-91	32.76376933992982	34.0	32.0	35.0	27.0	36.0
92-93	32.70177982248897	34.0	32.0	35.0	27.0	36.0
94-95	32.64558022710604	34.0	32.0	35.0	27.0	35.5
96-97	32.632345021479	34.0	32.0	35.0	27.5	35.0
98-99	32.74615621667293	34.0	32.0	35.0	29.0	35.0
100-101	32.12578696461183	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	0.0
16	1.0
17	2.0
18	1.0
19	10.0
20	82.0
21	528.0
22	2272.0
23	7640.0
24	20288.0
25	42380.0
26	75838.0
27	118220.0
28	169260.0
29	224620.0
30	284548.0
31	349029.0
32	428399.0
33	530388.0
34	678274.0
35	883356.0
36	1229627.0
37	1818425.0
38	2068094.0
39	888774.0
40	33688.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.65149189470603	22.615990164145714	15.610399903792924	24.122118037355342
2	13.46028468095281	23.162753179754635	41.65425793411247	21.722704205180086
3	16.41530199939211	28.451030547269085	32.05208781026909	23.081579643069716
4	11.668741174040937	19.916468307227355	40.420469577810266	27.994320940921448
5	12.005965651834028	38.96681697503172	36.73447744679357	12.29273992634068
6	27.55171764643798	40.42976553584449	19.632535650151286	12.38598116756624
7	24.244376122986743	33.721168956574374	25.210962938456394	16.823491981982485
8	23.156911407794702	37.87055581405851	22.363649556589905	16.60888322155688
9	25.089953109198582	16.729893050814688	22.267533815823324	35.9126200241634
10-11	23.06678323825104	27.678309109886644	30.303255259802235	18.951652392060076
12-13	23.993146768056206	25.42863652347407	30.13883046496535	20.439386243504373
14-15	21.238473291119263	26.69563196530862	27.790124465368244	24.275770278203872
16-17	20.643430492670554	29.632642208622208	28.48343447085347	21.240492827853775
18-19	20.69394430103246	28.94471179180737	30.093092428261603	20.268251478898563
20-21	21.630653703657657	28.168233252969987	29.78664216015869	20.414470883213667
22-23	21.286609012641534	28.05658810861244	29.679453114179626	20.977349764566398
24-25	21.044740552354654	28.249367801398765	29.731869206115324	20.974022440131254
26-27	21.038891315610368	28.380197275525443	29.602929673611605	20.977981735252587
28-29	20.977509314328987	28.496485318999444	29.609422745877502	20.91658262079407
30-31	21.293483248928343	28.30840108094967	29.489338238118794	20.90877743200319
32-33	21.10384085171609	28.49885569422713	29.405051799833004	20.99225165422377
34-35	21.199853067056278	28.529986677547402	29.461805880819075	20.80835437457724
36-37	21.175912276511813	28.43992686964184	29.453153952580564	20.931006901265782
38-39	20.999609119089406	28.561436438881564	29.496891535238294	20.94206290679074
40-41	21.16755065289825	28.32201119203528	29.44316344468001	21.067274710386457
42-43	21.17651917182193	28.41749233369855	29.212493809533168	21.19349468494635
44-45	21.11281812600528	28.543196714681436	29.054760171479415	21.289224987833872
46-47	21.159815918545267	28.431437745407617	28.875225457522173	21.533520878524946
48-49	21.08304004288297	28.72662774369965	28.88359997013461	21.30673224328277
50-51	21.09184446460829	28.797171020319283	28.7392498640693	21.371734651003127
52-53	21.163064767089278	28.657881382438983	28.4332294191541	21.745824431317644
54-55	21.133873633815586	28.349798492981805	28.68522337149712	21.831104501705482
56-57	21.144997203854516	28.11938056736495	28.89851808720606	21.837104141574475
58-59	20.973112222684527	27.910539661111304	29.072007605390677	22.044340510813498
60-61	21.120291160853206	27.642124184410715	29.35409190820515	21.883492746530926
62-63	20.82394525916721	28.229816899300026	29.33410579692632	21.612132044606447
64-65	20.38622297185709	28.45457965381334	29.131251996328494	22.027945378001075
66-67	20.544066061289527	29.14207586567507	28.517684022683753	21.79617405035165
68-69	20.596573611943427	29.485116512096653	28.07673245118683	21.84157742477309
70-71	20.609108990455148	29.013463689357096	28.039618187051442	22.337809133136314
72-73	20.539383228629575	28.57998158358449	28.010391682181506	22.870243505604428
74-75	20.95369046168761	28.22804977466814	28.075882436790895	22.742377326853358
76-77	21.376192998275123	28.04473430662257	27.98478697375357	22.59428572134873
78-79	21.641149740737713	27.950012733743847	27.234483567826327	23.174353957692112
80-81	21.555812739258094	28.367016222005386	27.129864921215457	22.94730611752106
82-83	21.470597796474706	28.443754595318616	27.315551665530613	22.770095942676065
84-85	21.49770298844089	28.499997466882114	27.21398944288579	22.788310101791204
86-87	21.771468247809963	28.84435085541208	27.183943029608976	22.20023786716898
88-89	21.97891657491051	28.472049379579346	27.590079376005423	21.958954669504724
90-91	21.71712928133428	28.452111010854512	27.898080290672215	21.93267941713899
92-93	21.471453441506604	27.894220754189043	27.75667184552647	22.877653958777884
94-95	21.273019457578506	27.890676979918027	28.510228595109915	22.32607496739355
96-97	20.735846987547166	28.21876004718667	28.874897361825415	22.170495603440756
98-99	20.937167669020994	28.3021913413104	29.239395123663826	21.521245866004776
100-101	21.543647234087953	31.386462156566836	26.660249531826963	20.409641077518245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2815.0
1	2418.0
2	3040.5
3	4260.0
4	5553.0
5	8344.0
6	10653.5
7	12617.5
8	14106.5
9	14143.0
10	13227.5
11	12321.0
12	13467.5
13	17644.5
14	27513.0
15	40906.5
16	51600.0
17	57483.0
18	58814.0
19	56958.5
20	53949.0
21	51791.0
22	52134.0
23	57300.5
24	68058.0
25	84745.5
26	106846.5
27	130674.0
28	156655.5
29	184511.0
30	209995.0
31	235172.0
32	261550.0
33	287642.5
34	312627.0
35	335127.5
36	359103.5
37	380186.0
38	393000.0
39	402645.5
40	411613.0
41	417326.5
42	417998.0
43	419542.0
44	420163.0
45	417227.0
46	411290.0
47	402809.0
48	392946.5
49	378635.5
50	361536.5
51	341475.5
52	317018.0
53	291642.0
54	267576.0
55	244296.5
56	219639.5
57	193110.5
58	168346.0
59	145048.0
60	120835.0
61	96466.5
62	74329.0
63	56284.0
64	41776.0
65	31313.0
66	23801.0
67	17873.5
68	13724.5
69	9886.0
70	6758.5
71	4817.0
72	3378.0
73	2383.5
74	1641.0
75	1069.5
76	712.0
77	487.5
78	345.5
79	254.0
80	185.5
81	135.0
82	101.5
83	71.0
84	50.5
85	37.0
86	30.5
87	26.0
88	20.5
89	15.5
90	10.5
91	8.5
92	7.5
93	4.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00475961170093198
3	0.0
4	0.0
5	2.0296851603121453E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.4145652236789163E-4
20-21	7.104265756373414E-4
22-23	2.2330603467810763E-4
24-25	1.3199343078848307E-4
26-27	0.002209396436065781
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.034293826313732685
38-39	0.017226685088785578
40-41	0.021556373276160844
42-43	0.010795702081280027
44-45	0.004455189938271753
46-47	0.005174958371079694
48-49	0.008804851473161712
50-51	0.006927494975649444
52-53	0.010693415581774226
54-55	0.003947059866063102
56-57	0.01585597304695035
58-59	0.012511681499528634
60-61	0.012431842188378686
62-63	0.004886369368381808
64-65	0.0063616230415706404
66-67	5.078770577389267E-4
68-69	8.656142123559877E-4
70-71	0.001108847231152132
72-73	1.1422324705189799E-4
74-75	0.0040890747965517706
76-77	0.005123071440149765
78-79	0.0041952805915282925
80-81	7.709080455786321E-4
82-83	0.00338020113880401
84-85	0.004487036051492489
86-87	0.0014983534166203782
88-89	0.07321724587244671
90-91	9.929887356885498E-4
92-93	7.092708652948517E-6
94-95	5.300063070750542E-4
96-97	0.012353898715657619
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	305.0
20-21	1035.0
22-23	2134.0
24-25	4604.0
26-27	5294.0
28-29	8411.0
30-31	23424.0
32-33	30755.0
34-35	44850.0
36-37	65574.0
38-39	51069.0
40-41	78177.0
42-43	86426.0
44-45	101050.0
46-47	104185.0
48-49	94038.0
50-51	86473.0
52-53	17008.0
54-55	16648.0
56-57	16452.0
58-59	17299.0
60-61	102128.0
62-63	99625.0
64-65	105125.0
66-67	113863.0
68-69	116425.0
70-71	116257.0
72-73	112144.0
74-75	114000.0
76-77	114987.0
78-79	123925.0
80-81	127863.0
82-83	122089.0
84-85	123370.0
86-87	130980.0
88-89	138507.0
90-91	150025.0
92-93	156919.0
94-95	183986.0
96-97	244407.0
98-99	892043.0
100-101	5609866.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.0221667399646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64914742604014	77.97836074718458
2	6.601206189905656	11.357001191059016
3	1.496790573578367	3.8627150488549664
4	0.5604552713565316	1.9284630721169465
5	0.2543313896875444	1.0939068605454427
6	0.14134895907468348	0.7295486235625713
7	0.08319754254094704	0.500978301376885
8	0.05522007965560098	0.3800120719622588
9	0.03457499507442759	0.2676794392193279
>10	0.1203478597538718	1.607405873275558
>50	0.002736050176966263	0.15533304380082852
>100	5.885243249298697E-4	0.09331444379199408
>500	1.8379880345265736E-5	0.010561595239896596
>1k	3.675894992095192E-5	0.03471968800969956
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0148425801560726E-5
2	0.0	3.044527740468218E-5	0.0	0.0	1.0148425801560726E-5
3	0.0	3.044527740468218E-5	0.0	0.0	1.0148425801560726E-5
4	0.0	5.074212900780363E-5	0.0	1.0148425801560726E-5	1.0148425801560726E-5
5	0.0	7.103898061092508E-5	0.0	3.044527740468218E-5	1.0148425801560726E-5
6	0.0	7.103898061092508E-5	0.0	3.044527740468218E-5	3.044527740468218E-5
7	0.0	7.103898061092508E-5	0.0	3.044527740468218E-5	3.044527740468218E-5
8	0.0	7.103898061092508E-5	0.0	3.044527740468218E-5	3.044527740468218E-5
9	0.0	1.2178110961872872E-4	0.0	5.074212900780363E-5	4.0593703206242906E-5
10-11	0.0	1.2178110961872872E-4	0.0	6.089055480936436E-5	4.0593703206242906E-5
12-13	0.0	1.2178110961872872E-4	0.0	6.089055480936436E-5	4.566791610702327E-5
14-15	0.0	1.8774587732887346E-4	0.0	6.089055480936436E-5	6.596476771014472E-5
16-17	0.0	1.928200902296538E-4	0.0	7.103898061092508E-5	7.103898061092508E-5
18-19	0.0	2.0296851603121454E-4	0.0	1.1163268381716799E-4	8.118740641248581E-5
20-21	0.0	2.1311694183277525E-4	0.0	1.8267166442809308E-4	8.118740641248581E-5
22-23	0.0	2.5371064503901817E-4	0.0	3.856401804593076E-4	9.133583221404654E-5
24-25	0.0	2.993785611460414E-4	0.0	0.001131549476874021	1.1163268381716799E-4
26-27	0.0	3.0952698694760217E-4	0.0	0.0034859842628361098	1.1670689671794835E-4
28-29	0.0	3.196754127491629E-4	0.0	0.014918185928294268	1.2178110961872872E-4
30-31	0.0	3.450464772530647E-4	0.0	0.02778131563177249	1.2178110961872872E-4
32-33	0.0	3.450464772530647E-4	0.0	0.05128506978818713	1.2178110961872872E-4
34-35	0.0	4.414565223678916E-4	0.0	0.08266907657951367	1.3192953542028943E-4
36-37	0.0	5.226439287803774E-4	0.0	0.12216167558628724	1.3192953542028943E-4
38-39	0.0	5.581634190858399E-4	0.0	0.19933030538135502	1.4207796122185016E-4
40-41	0.0	6.697961029030079E-4	0.0	0.5344769932649972	1.4207796122185016E-4
42-43	0.0	6.799445287045687E-4	0.0	0.600370721994531	1.4715217412263054E-4
44-45	0.0	7.509835093154937E-4	0.0	0.7043362701186199	1.522263870234109E-4
46-47	0.0	8.118740641248581E-4	0.0	0.8213983617396229	1.5730059992419125E-4
48-49	0.0	8.169482770256384E-4	0.0	0.9174379893126928	1.7252323862653235E-4
50-51	0.0	8.829130447357831E-4	0.0	1.0089057510621595	1.7252323862653235E-4
52-53	0.0	8.930614705373439E-4	0.0	1.2601249575668947	1.7252323862653235E-4
54-55	0.0	9.285809608428065E-4	0.0	2.048723607115873	1.7252323862653235E-4
56-57	0.0	9.539520253467082E-4	0.0	2.9115529171903676	1.7252323862653235E-4
58-59	0.0	9.7932308985061E-4	0.0	3.8102315414088754	1.7252323862653235E-4
60-61	0.0	9.945457285529511E-4	0.0	4.49580844643331	1.8267166442809308E-4
62-63	0.0	9.945457285529511E-4	0.0	4.5144764756952815	1.8267166442809308E-4
64-65	0.0	0.0011112526252708994	0.0	4.525279474961042	1.8774587732887346E-4
66-67	0.0	0.0011264752639732407	0.0	4.533920859531072	1.9789430313043416E-4
68-69	0.0	0.0011366236897748013	0.0	4.541313987727508	2.0296851603121452E-4
70-71	0.0	0.0011518463284771424	0.0	4.548225065698372	2.0296851603121452E-4
72-73	0.0	0.0011670689671794835	0.0	4.554055336321368	2.0296851603121452E-4
74-75	0.0	0.0012076626703857265	0.0	4.559378185654287	2.0296851603121452E-4
76-77	0.0	0.0012431821606911889	0.0	4.564766999754916	2.1311694183277525E-4
78-79	0.0	0.0012584047993935302	0.0	4.569120674423785	2.2326536763433598E-4
80-81	0.0	0.0012584047993935302	0.0	4.571607038745167	2.2326536763433598E-4
82-83	0.0	0.0012634790122943106	0.0	4.573058263634791	2.334137934358967E-4
84-85	0.0	0.0013091469284013339	0.0	4.574585601717925	2.334137934358967E-4
86-87	0.0	0.0013497406316075767	0.0	4.575123468285408	2.334137934358967E-4
88-89	0.0	0.0014004827606153803	0.0	4.575174210414416	2.334137934358967E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11640	0.0	50.468113	1
TATCTCG	11785	0.0	37.700336	94-95
GTATCAA	21260	0.0	36.60348	1
CCGTCTT	4395	0.0	31.992533	92-93
TATGCCG	4325	0.0	30.205563	88-89
TGCCGTC	4525	0.0	29.554043	90-91
TCAACGC	26270	0.0	28.929316	4
ATCAACG	26380	0.0	28.776623	3
GCCGTCT	4615	0.0	28.572409	90-91
CAACGCA	26755	0.0	28.357481	5
ATGCCGT	4560	0.0	28.05068	88-89
AACGCAG	27430	0.0	27.70591	6
TATCAAC	27970	0.0	27.47453	2
CGTATGC	5370	0.0	25.04772	86-87
TCGTATG	5305	0.0	24.703224	84-85
CTCGTAT	5435	0.0	24.539112	84-85
CTATCTC	20060	0.0	24.31843	94-95
ATCTCGT	9460	0.0	24.316017	94-95
ACGCAGA	31880	0.0	23.85182	7
CGCAGAG	32305	0.0	23.498756	8
>>END_MODULE