FastQCFastQC Report
Fri 27 May 2016
SRR2139801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11147786
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130650.048.2012831
GTATCAA238300.035.5778351
TCAACGC297550.027.8651124
ATCAACG297950.027.813793
CAACGCA304100.027.183135
AACGCAG313900.026.3872996
TATCAAC318650.026.2293072
ACGCAGA363550.022.7835877
CGCAGAG366100.022.5909218
GTACATG338100.020.1834261
TACATGG332800.020.1685072
ACATGGG333500.019.5783213
GCAGAGT431800.018.961619
AGAGTAC399350.018.01613610-11
GAGTACT254300.017.62276312-13
CATGGGG231950.017.4618764
CAGAGTA429600.016.76684410-11
AGTACTT281250.015.74985812-13
GTACTTT292500.015.33684314-15
ATGGGGG147000.015.1724495
Produced by FastQC (version 0.11.5)