##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11147786
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.826267386187716	31.0	31.0	34.0	30.0	34.0
2	31.985682986738354	31.0	31.0	34.0	30.0	34.0
3	32.0097978199438	31.0	31.0	34.0	30.0	34.0
4	35.72735303673752	37.0	35.0	37.0	35.0	37.0
5	35.544292561769666	37.0	35.0	37.0	33.0	37.0
6	35.550750615413676	37.0	35.0	37.0	33.0	37.0
7	35.442619189137645	37.0	35.0	37.0	33.0	37.0
8	35.42386918801635	37.0	35.0	37.0	33.0	37.0
9	36.96043456521322	39.0	37.0	39.0	33.0	39.0
10-11	36.85008023117774	39.0	37.0	39.0	33.0	39.0
12-13	36.793604487922536	39.0	37.0	39.0	32.0	39.0
14-15	37.90235612703724	40.0	37.0	41.0	33.0	41.0
16-17	37.891876198556375	40.0	37.0	41.0	33.0	41.0
18-19	37.935260239118335	40.0	37.0	41.0	33.0	41.0
20-21	37.918971860744236	40.0	37.0	41.0	32.5	41.0
22-23	37.851686029397555	40.0	37.0	41.0	32.0	41.0
24-25	37.76458744114052	39.5	37.0	41.0	32.0	41.0
26-27	37.57662161882605	39.0	36.5	41.0	32.0	41.0
28-29	37.47497809372006	39.0	36.0	41.0	32.0	41.0
30-31	37.32803946981308	39.0	36.0	40.5	31.5	41.0
32-33	37.48441478645954	39.0	36.0	41.0	32.0	41.0
34-35	37.440367145964835	39.0	36.0	41.0	31.5	41.0
36-37	37.353870269325334	39.0	36.0	41.0	31.0	41.0
38-39	37.25024296230279	39.0	36.0	41.0	31.0	41.0
40-41	37.16425565883925	39.0	36.0	41.0	31.0	41.0
42-43	37.12618042213943	39.0	36.0	40.0	31.0	41.0
44-45	37.15605500687124	39.0	36.0	40.5	31.0	41.0
46-47	37.27018251937208	39.0	36.0	41.0	31.0	41.0
48-49	37.22393026244868	39.0	36.0	41.0	31.0	41.0
50-51	37.125035369733666	39.0	36.0	41.0	31.0	41.0
52-53	37.039787627686735	39.0	35.5	40.5	31.0	41.0
54-55	36.929026276390964	39.0	35.0	40.0	31.0	41.0
56-57	36.81666005769283	39.0	35.0	40.0	30.5	41.0
58-59	36.691540798968155	38.5	35.0	40.0	30.0	41.0
60-61	36.49154284876085	38.0	35.0	40.0	30.0	41.0
62-63	36.29345285365575	38.0	34.5	40.0	30.0	41.0
64-65	36.108978681645546	38.0	34.0	40.0	29.0	41.0
66-67	35.871699116531424	37.5	34.0	40.0	29.0	41.0
68-69	35.609816106940414	37.0	34.0	40.0	29.0	41.0
70-71	35.3299264720539	36.5	34.0	39.0	29.0	41.0
72-73	35.03453501712641	36.0	34.0	39.0	29.0	40.0
74-75	34.68715896979563	36.0	33.0	38.5	28.0	40.0
76-77	33.62334138219849	35.0	31.5	37.0	27.0	39.0
78-79	34.03565236936264	35.0	33.0	37.0	28.0	39.0
80-81	33.92037857482139	35.0	33.0	37.0	29.0	39.0
82-83	33.667152118174386	35.0	33.0	36.0	28.0	38.5
84-85	33.417958552816046	35.0	32.0	36.0	28.0	37.0
86-87	33.188884341046354	35.0	32.0	35.5	28.0	37.0
88-89	32.96012447405286	34.0	32.0	35.0	28.0	36.5
90-91	32.81819973713094	34.0	32.0	35.0	27.0	36.0
92-93	32.7268642277002	34.0	32.0	35.0	27.5	36.0
94-95	32.674040471510146	34.0	32.0	35.0	28.0	36.0
96-97	32.67479535565438	34.0	32.0	35.0	28.5	35.0
98-99	32.78183991453513	34.0	32.0	35.0	29.0	35.0
100-101	32.15164618433994	33.5	31.0	35.0	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	3.0
19	12.0
20	111.0
21	660.0
22	2734.0
23	9034.0
24	22677.0
25	47580.0
26	83868.0
27	131150.0
28	187208.0
29	249509.0
30	317658.0
31	392872.0
32	485626.0
33	603764.0
34	773464.0
35	1005919.0
36	1387985.0
37	2027394.0
38	2301320.0
39	1080872.0
40	36366.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.19613921544601	23.12198135127459	15.773266548173781	23.908612885105615
2	13.195497446346726	23.53707329706586	42.02258417528881	21.2448450812986
3	16.140119661428738	29.095212269055036	32.32449026201257	22.440177807503662
4	11.408049993065887	20.337769311323342	41.01757963419821	27.236601061412554
5	11.671252331405057	39.32076545437192	36.99739786849117	12.01058434573185
6	26.831955690573896	41.12072119073689	19.92909623489364	12.11822688379558
7	23.673498935124876	34.05539898236295	25.695272585964602	16.575829496547563
8	22.77753627491593	38.26025185628787	22.630134808831098	16.33207705996509
9	24.900011535922918	16.844133893492394	22.5534828171262	35.702371753458486
10-11	22.7929653475587	27.892668553199712	30.634952985283352	18.679413113958233
12-13	23.764090914554693	25.66223015045319	30.482335236790515	20.091343698201598
14-15	21.006215942789	26.891842021366397	28.159649817461514	23.942292218383095
16-17	20.31522671856098	29.906359881684132	28.805562826555875	20.97285057319902
18-19	20.370902947960488	29.175301076693476	30.463893985337474	19.98990199000856
20-21	21.328975460253137	28.39219619740632	30.148429454944708	20.130398887395838
22-23	20.99422791259968	28.311934155972462	30.02382647553748	20.670011455890382
24-25	20.773902229526577	28.476235209563704	30.099656634499343	20.65020592641037
26-27	20.724120940681676	28.62529439068503	29.965341662014396	20.685243006618904
28-29	20.66077810543924	28.73312007707525	29.952328020527162	20.65377379695835
30-31	21.012544780696334	28.54634120797583	29.837483743358277	20.603630267969557
32-33	20.82741502181248	28.72163253243048	29.744570268222258	20.706382177534778
34-35	20.898212000911474	28.792829245403528	29.809984074505714	20.498974679179284
36-37	20.899849603910845	28.729194009134456	29.73732661257068	20.63362977438402
38-39	20.740932520363007	28.807125348699834	29.7725895173931	20.679352613544058
40-41	20.970082329440785	28.67879308120048	29.645476043247353	20.705648546111377
42-43	20.835741575383675	28.801353885449092	29.409798786535323	20.95310575263191
44-45	20.953560735857547	28.946657103119268	29.215491607779704	20.884290553243485
46-47	20.97676353853832	28.920228385147524	29.014642912015603	21.08836516429855
48-49	20.922098929910064	29.0376480761	29.022918014970976	21.017334979018955
50-51	20.98060710251038	29.119811240947936	28.80346038736395	21.096121269177736
52-53	21.0453679306415	29.00916303743411	28.769835417686203	21.175633614238183
54-55	21.05055549511495	28.962264945506522	28.782115951304355	21.20506360807417
56-57	21.083252052567214	29.044338294589316	28.733602232335855	21.138807420507614
58-59	21.02651644347108	29.119506875381468	28.75179411879902	21.102182562348435
60-61	21.163504392995467	29.078452965668745	28.717598383974362	21.040444257361425
62-63	21.11202145752891	29.132179201158486	28.67520189548887	21.08059744582374
64-65	21.0351840899189	29.073705182440385	28.683079645826933	21.208031081813786
66-67	21.167109528107414	29.110550659134997	28.52671610301762	21.19562370973997
68-69	21.07368437030897	29.07942108119273	28.66775705013144	21.179137498366867
70-71	21.105686489742904	28.845755759018555	28.721002903900832	21.32755484733771
72-73	21.169245060575435	28.90038623242094	28.677208296768637	21.253160410234987
74-75	20.992427548975158	29.022112799998872	28.663482875469125	21.321976775556845
76-77	21.085388971695664	28.96757305474049	28.6301388619704	21.316899111593447
78-79	21.10789221672034	28.929404483998166	28.627647667555255	21.335055631726238
80-81	21.132504889978943	28.924998392105117	28.621249478135496	21.321247239780448
82-83	21.123110646446012	28.871171747812348	28.76968949829195	21.23602810744969
84-85	21.240613026212316	28.837999705640655	28.67184465048243	21.2495426176646
86-87	21.019465161642927	28.89758681205132	28.738046573670463	21.344901452635295
88-89	21.129384222909152	28.8181126478437	28.71338433408705	21.339118795160097
90-91	21.132783143171277	28.785540462429793	28.826866188637233	21.2548102057617
92-93	21.152732621800176	28.698964833909564	28.826072848176693	21.322229696113563
94-95	20.959896033981106	28.74360893038822	28.912306121688307	21.384188913942364
96-97	20.890997901621837	28.781006373059814	29.019377592565565	21.308618132752784
98-99	21.025250587971982	29.41309408844336	29.05355719235736	20.5080981312273
100-101	21.466055282404174	32.19120963214949	26.458632889712952	19.884102195733387
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2671.0
1	2395.0
2	3448.0
3	5021.5
4	6621.0
5	10016.0
6	12785.5
7	14952.0
8	16402.5
9	16313.0
10	15318.5
11	14395.0
12	15873.0
13	20724.5
14	32283.0
15	48380.5
16	61398.5
17	68387.5
18	70223.0
19	69074.5
20	66915.0
21	66201.5
22	68873.5
23	77053.5
24	92554.5
25	115269.0
26	144068.5
27	176555.0
28	210161.5
29	244224.0
30	276502.5
31	309081.5
32	342639.0
33	372177.5
34	398082.0
35	422657.0
36	448657.5
37	470077.0
38	480860.5
39	484217.0
40	486900.0
41	488072.5
42	483181.5
43	478223.5
44	473013.5
45	465039.5
46	455644.0
47	442919.5
48	426704.0
49	406661.5
50	386561.0
51	365577.5
52	338720.5
53	309550.5
54	281788.5
55	255765.5
56	231541.0
57	205278.5
58	178799.0
59	154360.0
60	128781.0
61	103614.0
62	80481.0
63	60741.5
64	44676.0
65	33326.5
66	25440.0
67	19408.5
68	14937.5
69	10530.0
70	7047.0
71	5158.0
72	3677.0
73	2523.5
74	1727.0
75	1218.5
76	841.5
77	568.5
78	439.0
79	327.5
80	220.5
81	154.0
82	118.0
83	79.5
84	54.5
85	43.0
86	33.0
87	30.5
88	26.0
89	18.5
90	13.0
91	10.5
92	9.0
93	4.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003794475423191654
3	0.0
4	0.0
5	1.794078214275014E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.036675982118781E-4
20-21	6.862674518822231E-4
22-23	2.3327129662639015E-4
24-25	1.4808532407126942E-4
26-27	0.0021012231004157458
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03331416623723454
38-39	0.016207438338491365
40-41	0.021316657326179076
42-43	0.010759519421819075
44-45	0.004431737406546327
46-47	0.005267506299536746
48-49	0.008675065082298223
50-51	0.006823773891567499
52-53	0.010731426617903116
54-55	0.0037625144719761945
56-57	0.016041042491375987
58-59	0.012809122052984076
60-61	0.01280970308687523
62-63	0.004867168597780216
64-65	0.006503662312101087
66-67	4.493772033191642E-4
68-69	6.125915316105633E-4
70-71	9.729044997492709E-4
72-73	1.1146344706729082E-4
74-75	0.0034972454813215455
76-77	0.004296588583139189
78-79	0.003638853656466638
80-81	6.968972422063412E-4
82-83	0.003223365159612399
84-85	0.0044341018662628524
86-87	0.0013145511260556559
88-89	0.07292622325486153
90-91	8.55984198402978E-4
92-93	1.3147969749677119E-5
94-95	4.776837906886511E-4
96-97	0.01183273003709457
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	321.0
20-21	1150.0
22-23	2384.0
24-25	5853.0
26-27	6978.0
28-29	10671.0
30-31	27739.0
32-33	35708.0
34-35	50281.0
36-37	78168.0
38-39	92211.0
40-41	95131.0
42-43	106402.0
44-45	123427.0
46-47	124638.0
48-49	116092.0
50-51	106631.0
52-53	103832.0
54-55	109999.0
56-57	115536.0
58-59	117005.0
60-61	115833.0
62-63	109821.0
64-65	115179.0
66-67	122496.0
68-69	125881.0
70-71	126504.0
72-73	121953.0
74-75	121732.0
76-77	123675.0
78-79	133937.0
80-81	137268.0
82-83	132919.0
84-85	132798.0
86-87	140151.0
88-89	149236.0
90-91	161470.0
92-93	168836.0
94-95	196520.0
96-97	263364.0
98-99	880097.0
100-101	6137959.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.14878618950453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68454038841563	78.12363080615106
2	6.547360999609745	11.28094405721761
3	1.5591347845292145	4.029527075790796
4	0.5504002113612312	1.896652405088672
5	0.24475628950316985	1.0542728626472524
6	0.1334887865421359	0.6899938158308926
7	0.08134912995343302	0.4905690162142364
8	0.04349535170780402	0.2997657403610327
9	0.03391580344986541	0.2629624769862943
>10	0.1184215558690861	1.5920169036565655
>50	0.002371417130856521	0.13655273470832288
>100	6.830598766961414E-4	0.09568317099155664
>500	8.222205112981127E-5	0.04742893435576398
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	6.279273749962549E-5	0.0	8.970391071375069E-6	0.0
3	0.0	6.279273749962549E-5	0.0	2.691117321412521E-5	0.0
4	0.0	1.0764469285650084E-4	0.0	3.5881564285500275E-5	0.0
5	0.0	1.0764469285650084E-4	0.0	3.5881564285500275E-5	0.0
6	2.691117321412521E-5	1.0764469285650084E-4	0.0	3.5881564285500275E-5	0.0
7	2.691117321412521E-5	1.0764469285650084E-4	0.0	4.485195535687535E-5	0.0
8	2.691117321412521E-5	1.166150839278759E-4	0.0	5.382234642825042E-5	8.970391071375069E-6
9	2.691117321412521E-5	2.063189946416266E-4	0.0	7.176312857100055E-5	8.970391071375069E-6
10-11	2.691117321412521E-5	2.197745812486892E-4	0.0	7.624832410668808E-5	8.970391071375069E-6
12-13	2.691117321412521E-5	2.2425977678437674E-4	0.0	9.867430178512576E-5	8.970391071375069E-6
14-15	3.1396368749812744E-5	2.825673187483147E-4	0.0	1.0764469285650083E-4	2.2425977678437675E-5
16-17	3.5881564285500275E-5	2.870525142840022E-4	0.0	1.166150839278759E-4	2.691117321412521E-5
18-19	3.5881564285500275E-5	2.960229053553773E-4	0.0	1.300706705349385E-4	3.5881564285500275E-5
20-21	3.5881564285500275E-5	3.1844888303381494E-4	0.0	1.4801145267768866E-4	4.036675982118781E-5
22-23	3.5881564285500275E-5	3.633008383906903E-4	0.0	1.5698184374906372E-4	5.382234642825042E-5
24-25	3.5881564285500275E-5	4.126379892832532E-4	0.0	2.60141341069877E-4	7.176312857100055E-5
26-27	3.5881564285500275E-5	4.350639669616909E-4	0.0	5.471938553538792E-4	8.073351964237563E-5
28-29	3.5881564285500275E-5	4.395491624973784E-4	0.0	0.002112527097308829	8.073351964237563E-5
30-31	4.485195535687535E-5	4.933715089256288E-4	0.0	0.006467651962461425	8.073351964237563E-5
32-33	5.382234642825042E-5	4.933715089256288E-4	0.0	0.01783313744989364	8.97039107137507E-5
34-35	5.382234642825042E-5	5.382234642825042E-4	0.0	0.031261812883742114	9.867430178512576E-5
36-37	5.382234642825042E-5	5.830754196393795E-4	0.0	0.0474937355273953	9.867430178512576E-5
38-39	5.382234642825042E-5	6.144717883891923E-4	0.0	0.07111726041386154	1.031594973208133E-4
40-41	5.382234642825042E-5	6.996905035672554E-4	0.0	0.09345353418158547	1.1212988839218837E-4
42-43	5.382234642825042E-5	7.310868723170681E-4	0.0	0.11195048057076087	1.2110027946356345E-4
44-45	5.382234642825042E-5	8.028500008880687E-4	0.0	0.13071205349654183	1.300706705349385E-4
46-47	5.382234642825042E-5	8.477019562449441E-4	0.0	0.14808770100179536	1.3455586607062604E-4
48-49	5.382234642825042E-5	8.521871517806316E-4	0.0	0.16067315967493456	1.435262571420011E-4
50-51	6.279273749962549E-5	9.104946937445696E-4	0.0	0.1718906337096891	1.524966482133762E-4
52-53	6.279273749962549E-5	9.149798892802571E-4	0.0	0.1817446083015946	1.7492262589181384E-4
54-55	6.279273749962549E-5	9.149798892802571E-4	0.0	0.1919170317765339	1.9734860357025153E-4
56-57	6.279273749962549E-5	9.508614535657573E-4	0.0	0.20094124519433723	2.0183379910593906E-4
58-59	6.279273749962549E-5	9.598318446371324E-4	0.0	0.20964252453357107	2.1528938571300165E-4
60-61	6.279273749962549E-5	9.777726267798826E-4	0.0	0.2181599108558417	2.332301678557518E-4
62-63	6.279273749962549E-5	9.867430178512576E-4	0.0	0.22580716924418892	2.4220055892712686E-4
64-65	6.279273749962549E-5	0.0011212988839218838	0.0	0.2334095756771793	2.466857544628144E-4
66-67	6.727793303531302E-5	0.0011302692749932588	0.0	0.24148292764141688	2.60141341069877E-4
68-69	8.073351964237563E-5	0.0011526952526716965	0.0	0.24951142765029757	2.691117321412521E-4
70-71	8.073351964237563E-5	0.0011796064258858215	0.0	0.2571317748654307	2.691117321412521E-4
72-73	8.073351964237563E-5	0.0011930620124928843	0.0	0.2634200190064646	3.049932964267524E-4
74-75	8.073351964237563E-5	0.001215487990171322	0.0	0.2691341581189305	3.049932964267524E-4
76-77	8.073351964237563E-5	0.0012558547499925098	0.0	0.27425176622515	3.049932964267524E-4
78-79	8.073351964237563E-5	0.0012648251410638848	0.0	0.27870556539208774	3.049932964267524E-4
80-81	8.073351964237563E-5	0.0012648251410638848	0.0	0.2807149329920757	3.049932964267524E-4
82-83	8.073351964237563E-5	0.0012693103365995723	0.0	0.28215468075903144	3.049932964267524E-4
84-85	8.073351964237563E-5	0.0012827659232066348	0.0	0.2833836243358098	3.049932964267524E-4
86-87	8.073351964237563E-5	0.0013186474874921352	0.0	0.28382765869384285	3.049932964267524E-4
88-89	8.073351964237563E-5	0.0013904106160631357	0.0	0.2838680254536641	3.1844888303381494E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13065	0.0	48.201283	1
GTATCAA	23830	0.0	35.577835	1
TCAACGC	29755	0.0	27.865112	4
ATCAACG	29795	0.0	27.81379	3
CAACGCA	30410	0.0	27.18313	5
AACGCAG	31390	0.0	26.387299	6
TATCAAC	31865	0.0	26.229307	2
ACGCAGA	36355	0.0	22.783587	7
CGCAGAG	36610	0.0	22.590921	8
GTACATG	33810	0.0	20.183426	1
TACATGG	33280	0.0	20.168507	2
ACATGGG	33350	0.0	19.578321	3
GCAGAGT	43180	0.0	18.96161	9
AGAGTAC	39935	0.0	18.016136	10-11
GAGTACT	25430	0.0	17.622763	12-13
CATGGGG	23195	0.0	17.461876	4
CAGAGTA	42960	0.0	16.766844	10-11
AGTACTT	28125	0.0	15.749858	12-13
GTACTTT	29250	0.0	15.336843	14-15
ATGGGGG	14700	0.0	15.172449	5
>>END_MODULE