FastQCFastQC Report
Fri 27 May 2016
SRR2139800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9568447
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98550.049.9127041
GTATCAA179700.034.2451551
TCAACGC229100.026.2574464
ATCAACG232100.025.900023
CAACGCA235050.025.5037235
AACGCAG242950.024.708886
TATCAAC246700.024.469552
ACGCAGA286650.020.9419927
CGCAGAG290550.020.6320788
TATACCG6950.019.8769595
AGAGTAC308400.018.38592310-11
GTACATG223350.017.1875211
GCAGAGT342800.017.084329
CAGAGTA335100.016.9646999
GAGTACT213350.016.8941312-13
TACATGG226250.016.7266792
ACATGGG228150.016.0916253
AGTACTT238150.015.98738612-13
GTACTTT240650.014.99614514-15
TATACTG55750.014.9426985
Produced by FastQC (version 0.11.5)