##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9568447
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.39437988212716	31.0	31.0	34.0	30.0	34.0
2	31.601035779369422	31.0	31.0	34.0	30.0	34.0
3	31.60781545845423	31.0	31.0	34.0	30.0	34.0
4	35.39825083422629	37.0	35.0	37.0	33.0	37.0
5	35.16054151734341	37.0	35.0	37.0	32.0	37.0
6	35.17370530452852	37.0	35.0	37.0	32.0	37.0
7	35.05818352758812	37.0	35.0	37.0	32.0	37.0
8	35.03884538420916	37.0	35.0	37.0	32.0	37.0
9	36.523057503479926	38.0	35.0	39.0	32.0	39.0
10-11	36.35852124174383	38.0	35.0	39.0	32.0	39.0
12-13	36.29798262978308	38.0	35.0	39.0	32.0	39.0
14-15	37.298889098722086	39.0	36.0	40.0	32.0	41.0
16-17	37.26401144302727	39.0	36.0	40.0	32.0	41.0
18-19	37.28220169897999	39.0	36.0	40.0	32.0	41.0
20-21	37.252222390342126	39.0	36.0	40.0	31.0	41.0
22-23	37.16800716615804	39.0	36.0	40.0	31.0	41.0
24-25	37.06769884091854	39.0	36.0	40.0	31.0	41.0
26-27	36.83426397884574	39.0	36.0	40.0	30.5	41.0
28-29	36.69741972090702	38.5	35.5	40.0	30.0	41.0
30-31	36.53050057917077	38.0	35.0	40.0	30.0	41.0
32-33	36.72079460783489	38.0	35.0	40.0	30.0	41.0
34-35	36.66348943765038	38.0	35.0	40.0	30.0	41.0
36-37	36.55182313891389	38.0	35.0	40.0	30.0	41.0
38-39	36.41562170346551	38.0	35.0	40.0	30.0	41.0
40-41	36.30582806028793	38.0	35.0	40.0	30.0	41.0
42-43	36.2330921971134	38.0	35.0	40.0	30.0	41.0
44-45	36.26558543808887	38.0	35.0	40.0	30.0	41.0
46-47	36.38999708663276	38.0	35.0	40.0	30.0	41.0
48-49	36.328426106521604	38.0	35.0	40.0	30.0	41.0
50-51	36.20942230826356	38.0	35.0	40.0	29.0	41.0
52-53	36.09187575393853	38.0	34.0	40.0	29.0	41.0
54-55	35.9462500256047	38.0	34.0	40.0	28.5	41.0
56-57	35.798621994028224	38.0	34.0	40.0	28.0	41.0
58-59	35.64195388977216	38.0	34.0	40.0	28.0	41.0
60-61	35.416644263317	37.5	34.0	40.0	28.0	41.0
62-63	35.18872742305493	37.0	33.0	40.0	28.0	41.0
64-65	34.9784094093881	37.0	33.0	39.0	28.0	40.5
66-67	34.71620169056376	36.0	33.0	39.0	27.0	40.0
68-69	34.4326932434132	36.0	32.0	39.0	26.5	40.0
70-71	34.14268725142183	35.5	32.0	38.5	26.0	40.0
72-73	33.83826532518137	35.0	32.0	38.0	26.0	40.0
74-75	33.48541089287521	35.0	31.0	37.0	26.0	39.5
76-77	32.536871968211045	34.0	30.5	36.0	26.0	39.0
78-79	32.98462241291339	34.0	31.0	36.0	26.0	39.0
80-81	32.89732451348637	34.0	31.0	36.0	26.0	38.5
82-83	32.67746250200306	34.0	31.0	36.0	26.0	37.0
84-85	32.456400910579205	34.0	31.0	35.0	26.0	37.0
86-87	32.24035272122848	34.0	31.0	35.0	26.0	36.5
88-89	32.03355215566045	34.0	31.0	35.0	26.0	36.0
90-91	31.914738312059374	34.0	31.0	35.0	26.0	36.0
92-93	31.843883512882933	34.0	31.0	35.0	26.0	35.5
94-95	31.823367196899316	34.0	31.0	35.0	26.0	35.0
96-97	31.86105613941428	34.0	31.0	35.0	26.0	35.0
98-99	32.047374258975736	34.0	31.0	35.0	26.5	35.0
100-101	31.445348621555038	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	21.0
20	157.0
21	1005.0
22	4326.0
23	13818.0
24	33975.0
25	67020.0
26	115120.0
27	171570.0
28	235394.0
29	299946.0
30	368105.0
31	442439.0
32	530871.0
33	646778.0
34	809439.0
35	1036508.0
36	1374191.0
37	1686547.0
38	1319490.0
39	407661.0
40	4059.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.58357453409106	24.571730396792706	17.00235158328201	23.84234348583422
2	14.160224968826618	24.798231318780147	39.48677601641009	21.554767695983145
3	16.83628492690611	28.69412350823493	32.38355189718875	22.086039667670207
4	12.657560835107306	21.226673461221033	38.809443162511116	27.306322541160544
5	12.219025720683826	38.94849394055274	36.573427223874475	12.259053114888967
6	28.05039313067209	40.94914253065309	18.783831900829885	12.216632437844929
7	24.180538388308992	33.557002510438735	25.170197420751766	17.092261680500503
8	23.212011311762502	38.31067883847818	21.838214707151536	16.639095142607783
9	24.925904903899244	16.312448613656947	21.84964811949107	36.91199836295274
10-11	23.22576484982359	27.6564838578298	30.16876719910765	18.948984093238955
12-13	24.102741019519677	25.279253780681444	30.045163023842846	20.572842175956037
14-15	21.31176563971144	26.594420181247802	27.468579801926058	24.625234377114698
16-17	20.93909805844146	29.47071243640687	28.212284605850872	21.377904899300795
18-19	20.99907900082694	28.73262936772427	29.7969647525125	20.471326878936292
20-21	21.746950369672273	28.003823031817454	29.567897486140755	20.68132911236952
22-23	21.467033601990998	27.957456952759035	29.41496959601834	21.160539849231625
24-25	21.31650850913654	28.115117768541033	29.411403027213552	21.156970695108882
26-27	21.36310045493318	28.200087109298945	29.309255483329494	21.127556952438386
28-29	21.351052015105815	28.219416741592568	29.34818105810113	21.08135018520049
30-31	21.555713568188928	28.113473101842963	29.2759106013713	21.05490272859681
32-33	21.446237236858693	28.235960544560694	29.18688285222068	21.130919366359933
34-35	21.53956575820885	28.238644646122594	29.206812509018544	21.01497708665001
36-37	21.50401049138788	28.2128605038373	29.153776154366017	21.129352850408797
38-39	21.388244436744568	28.28371997000775	29.218397895962926	21.10963769728476
40-41	21.568373135516257	28.193028148576115	29.068123246927712	21.170475468979916
42-43	21.47678715291354	28.352584375627966	28.827028059540073	21.343600411918423
44-45	21.581327988215296	28.384845666687795	28.644052085709237	21.389774259387668
46-47	21.637738413532038	28.37796930991432	28.458994754115537	21.525297522438112
48-49	21.552625321201578	28.508301489879624	28.422536590402615	21.516536598516183
50-51	21.607748087323593	28.554346270836987	28.261326202703458	21.576579439135966
52-53	21.694780509711844	28.46259432617806	28.178366897617362	21.664258266492734
54-55	21.675251205650785	28.37491662226224	28.26152917380841	21.688302998278566
56-57	21.655356301687707	28.417547517035217	28.235453510234088	21.691642671042988
58-59	21.652240779635846	28.440034536294075	28.231152414164768	21.676572269905314
60-61	21.75140231815297	28.417125064556565	28.205354261326853	21.626118355963616
62-63	21.68361566013697	28.490715401549505	28.197741088569277	21.62792784974425
64-65	21.622501053898198	28.448757961902043	28.191773791458985	21.73696719274077
66-67	21.706512740133675	28.560322386553715	27.96302352817118	21.77014134514143
68-69	21.687575638034183	28.50533235622618	27.99403357387335	21.81305843186628
70-71	21.693648806041974	28.37249143401251	28.07546179909826	21.858397960847253
72-73	21.73532394349775	28.374337637523723	27.96795832511397	21.92238009386456
74-75	21.7002938947947	28.432468758052025	27.939107019237586	21.92813032791569
76-77	21.847824591303244	28.36262856887221	27.9213496078821	21.86819723194245
78-79	21.81046849357609	28.369471258340063	27.84493087427922	21.97512937380463
80-81	21.811669377969846	28.389414546350828	27.86828957512399	21.930626500555334
82-83	21.81398387112351	28.34233700966802	27.981934244612095	21.861744874596372
84-85	21.847983610059675	28.395186981029237	27.933463877203103	21.823365531707985
86-87	21.804343218407165	28.36740846999896	27.972694287955157	21.855554023638714
88-89	21.765696686123356	28.35885428894102	28.019032840617708	21.856416184317915
90-91	21.832318777329412	28.31936515181046	28.11447826396452	21.733837806895608
92-93	21.867521104345734	28.278807011008205	28.10833104039942	21.745340844246634
94-95	21.685618255362893	28.31240446563977	28.208540768879953	21.793436510117388
96-97	21.520443284257556	28.385145416993808	28.33597833642678	21.758432962321855
98-99	21.629154328716407	28.999530670969165	28.41573950336798	20.955575496946448
100-101	22.17239308916753	31.743355362900488	25.713571565923814	20.37067998200817
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2508.0
1	2278.5
2	3092.5
3	4317.5
4	5413.5
5	7805.0
6	9919.0
7	11912.5
8	13471.0
9	13642.5
10	12975.0
11	12314.5
12	13696.5
13	18212.0
14	28815.0
15	43688.0
16	54992.0
17	59632.0
18	60238.5
19	58985.5
20	56510.5
21	54803.0
22	56049.0
23	62482.0
24	74882.5
25	92960.0
26	115438.5
27	138135.0
28	160670.0
29	183598.0
30	204525.5
31	225811.0
32	248497.0
33	269996.5
34	290620.0
35	310066.0
36	330875.0
37	350123.5
38	363277.0
39	373618.0
40	382703.5
41	388883.0
42	392940.0
43	396630.5
44	399568.5
45	400919.5
46	398045.5
47	391723.0
48	384752.0
49	374365.0
50	360544.0
51	344448.5
52	324657.5
53	303570.5
54	281416.5
55	256572.5
56	231405.5
57	206421.5
58	180320.5
59	153123.0
60	126410.5
61	102468.5
62	81234.5
63	62234.5
64	45651.5
65	33452.5
66	24929.0
67	18811.0
68	14228.5
69	10202.0
70	7206.0
71	5133.5
72	3497.0
73	2376.5
74	1665.5
75	1173.5
76	833.0
77	562.5
78	392.5
79	290.5
80	212.0
81	146.5
82	112.5
83	80.5
84	52.5
85	33.5
86	25.0
87	20.0
88	14.0
89	9.5
90	6.5
91	5.0
92	3.5
93	2.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.001964791151583951
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.605600783491825E-4
20-21	4.23293053776665E-4
22-23	1.6725415559936337E-4
24-25	1.4639353258469076E-4
26-27	0.0023853526791015383
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.029632690106406758
38-39	0.012783571944036811
40-41	0.019912087810806875
42-43	0.00752557236998521
44-45	0.002755701006222983
46-47	0.003873138005408749
48-49	0.0075350509063260365
50-51	0.00550763483259342
52-53	0.0092548227186664
54-55	0.0024414470547962823
56-57	0.014185485703488692
58-59	0.011187511945605433
60-61	0.010713463797425148
62-63	0.0038549328766495135
64-65	0.005219662185145202
66-67	2.493325337664665E-4
68-69	1.4174648431741549E-4
70-71	5.311437048660637E-4
72-73	7.604160362870531E-5
74-75	0.0011440215842166669
76-77	0.0012912070301136216
78-79	0.0010395483936924705
80-81	1.4138298955273962E-4
82-83	0.002130124147196921
84-85	0.003274870621707013
86-87	9.01165810535494E-4
88-89	0.06720487025384606
90-91	6.289600899664512E-4
92-93	1.5168248487460181E-5
94-95	3.275954470784765E-4
96-97	0.010632210118971685
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	353.0
20-21	1290.0
22-23	2525.0
24-25	4557.0
26-27	5794.0
28-29	8632.0
30-31	19708.0
32-33	26245.0
34-35	37535.0
36-37	55202.0
38-39	59727.0
40-41	66819.0
42-43	77390.0
44-45	90650.0
46-47	92339.0
48-49	84754.0
50-51	78972.0
52-53	71137.0
54-55	78838.0
56-57	84931.0
58-59	89812.0
60-61	91537.0
62-63	91072.0
64-65	100083.0
66-67	107867.0
68-69	111089.0
70-71	111660.0
72-73	110511.0
74-75	112599.0
76-77	112876.0
78-79	123537.0
80-81	127236.0
82-83	124133.0
84-85	127158.0
86-87	137203.0
88-89	146051.0
90-91	161937.0
92-93	174857.0
94-95	210444.0
96-97	281414.0
98-99	817067.0
100-101	5150906.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.86787494945914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.57766074026875	80.4673444208993
2	6.050144845144025	10.6322674135846
3	1.3552104294276046	3.572383816294428
4	0.48490883662300105	1.7043163607311025
5	0.21427788283223972	0.941407110656905
6	0.1043958962905542	0.5503827336297074
7	0.06169679210227258	0.3794816209257686
8	0.042443867863941574	0.29835619790721485
9	0.024764772808952663	0.19584251642959363
>10	0.08279238078295618	1.1305269883561888
>50	0.0014927081026604493	0.08642786427246268
>100	2.108477530386614E-4	0.04126295631272866
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.045101676374442E-5	0.0
2	0.0	5.22550838187221E-5	0.0	2.090203352748884E-5	0.0
3	0.0	5.22550838187221E-5	0.0	2.090203352748884E-5	0.0
4	0.0	1.045101676374442E-4	0.0	6.270610058246652E-5	0.0
5	0.0	1.2541220116493303E-4	0.0	6.270610058246652E-5	0.0
6	7.315711734621093E-5	1.2541220116493303E-4	0.0	9.405915087369978E-5	0.0
7	7.315711734621093E-5	1.2541220116493303E-4	0.0	1.1496118440118861E-4	0.0
8	8.360813410995536E-5	1.2541220116493303E-4	0.0	1.2541220116493303E-4	0.0
9	8.360813410995536E-5	2.090203352748884E-4	0.0	2.090203352748884E-4	0.0
10-11	8.360813410995536E-5	2.090203352748884E-4	0.0	2.6127541909361045E-4	0.0
12-13	8.360813410995536E-5	2.090203352748884E-4	0.0	2.874029610029715E-4	0.0
14-15	9.405915087369978E-5	2.2992236880237723E-4	0.0	3.1353050291233256E-4	1.045101676374442E-5
16-17	9.405915087369978E-5	2.2992236880237723E-4	0.0	3.3443253643982145E-4	2.090203352748884E-5
18-19	9.405915087369978E-5	2.5082440232986606E-4	0.0	3.5010906158543806E-4	2.090203352748884E-5
20-21	9.405915087369978E-5	2.717264358573549E-4	0.0	4.0758965378603234E-4	2.090203352748884E-5
22-23	1.045101676374442E-4	2.769519442392271E-4	0.0	5.016488046597321E-4	2.090203352748884E-5
24-25	1.1496118440118861E-4	3.2920702805794923E-4	0.0	9.98072100937592E-4	2.090203352748884E-5
26-27	1.1496118440118861E-4	3.553345699673103E-4	0.0	0.0020483992856939063	2.090203352748884E-5
28-29	1.1496118440118861E-4	3.553345699673103E-4	0.0	0.009944142450702815	2.090203352748884E-5
30-31	1.1496118440118861E-4	4.023641454041602E-4	0.0	0.018477397638300135	2.090203352748884E-5
32-33	1.2018669278306082E-4	4.0758965378603234E-4	0.0	0.03366272499602078	2.090203352748884E-5
34-35	1.3586321792867745E-4	4.4416821245913784E-4	0.0	0.0533263130370059	2.090203352748884E-5
36-37	1.4108872631054967E-4	4.911977878959877E-4	0.0	0.08054076068979638	2.090203352748884E-5
38-39	1.4631423469242186E-4	5.120998214234766E-4	0.0	0.12832280933363585	2.090203352748884E-5
40-41	1.4631423469242186E-4	5.539038884784542E-4	0.0	0.20396726866961795	2.090203352748884E-5
42-43	1.4631423469242186E-4	5.695804136240708E-4	0.0	0.24629388656278284	2.090203352748884E-5
44-45	1.5153974307429406E-4	6.270610058246651E-4	0.0	0.30614685956874715	2.090203352748884E-5
46-47	1.5676525145616628E-4	6.688650728796429E-4	0.0	0.3716486071355153	2.090203352748884E-5
48-49	1.619907598380385E-4	6.740905812615151E-4	0.0	0.4241127112895123	3.135305029123326E-5
50-51	2.0379482689301617E-4	7.263456650802372E-4	0.0	0.47279877288341565	3.135305029123326E-5
52-53	2.194713520386328E-4	7.367966818439815E-4	0.0	0.5311311229502551	3.135305029123326E-5
54-55	2.194713520386328E-4	7.629242237533427E-4	0.0	0.6385937028234572	3.135305029123326E-5
56-57	2.194713520386328E-4	7.942772740445759E-4	0.0	0.7502784934692119	3.135305029123326E-5
58-59	2.194713520386328E-4	8.204048159539369E-4	0.0	0.8397914520506828	3.135305029123326E-5
60-61	2.194713520386328E-4	8.256303243358091E-4	0.0	0.8971100534914391	3.135305029123326E-5
62-63	2.194713520386328E-4	8.308558327176814E-4	0.0	0.9476354940357614	3.135305029123326E-5
64-65	2.194713520386328E-4	9.405915087369978E-4	0.0	0.9696244333066797	3.135305029123326E-5
66-67	2.194713520386328E-4	9.405915087369978E-4	0.0	0.9792498197460884	3.657855867310547E-5
68-69	2.194713520386328E-4	9.510425255007421E-4	0.0	0.9878980361180869	4.180406705497768E-5
70-71	2.3514787718424945E-4	9.71944559028231E-4	0.0	0.9965148994397941	4.180406705497768E-5
72-73	2.717264358573549E-4	9.876210841738477E-4	0.0	1.0031669716099176	4.180406705497768E-5
74-75	2.717264358573549E-4	0.001018974134465081	0.0	1.0086851084611745	4.180406705497768E-5
76-77	2.717264358573549E-4	0.001066003709901931	0.0	1.0136597924407167	4.180406705497768E-5
78-79	2.717264358573549E-4	0.0010869057434294196	0.0	1.0180544449898714	4.180406705497768E-5
80-81	2.717264358573549E-4	0.0010973567601931641	0.0	1.0205835910466976	4.180406705497768E-5
82-83	2.717264358573549E-4	0.0011078077769569085	0.0	1.021916095684075	4.180406705497768E-5
84-85	2.717264358573549E-4	0.0011182587937206528	0.0	1.023144090153815	4.180406705497768E-5
86-87	2.769519442392271E-4	0.0011287098104843974	0.0	1.0236039348914199	4.180406705497768E-5
88-89	2.8217745262109934E-4	0.0011496118440118862	0.0	1.0236457389584748	5.22550838187221E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9855	0.0	49.912704	1
GTATCAA	17970	0.0	34.245155	1
TCAACGC	22910	0.0	26.257446	4
ATCAACG	23210	0.0	25.90002	3
CAACGCA	23505	0.0	25.503723	5
AACGCAG	24295	0.0	24.70888	6
TATCAAC	24670	0.0	24.46955	2
ACGCAGA	28665	0.0	20.941992	7
CGCAGAG	29055	0.0	20.632078	8
TATACCG	695	0.0	19.876959	5
AGAGTAC	30840	0.0	18.385923	10-11
GTACATG	22335	0.0	17.187521	1
GCAGAGT	34280	0.0	17.08432	9
CAGAGTA	33510	0.0	16.964699	9
GAGTACT	21335	0.0	16.89413	12-13
TACATGG	22625	0.0	16.726679	2
ACATGGG	22815	0.0	16.091625	3
AGTACTT	23815	0.0	15.987386	12-13
GTACTTT	24065	0.0	14.996145	14-15
TATACTG	5575	0.0	14.942698	5
>>END_MODULE