FastQCFastQC Report
Fri 27 May 2016
SRR2139799_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139799_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9206548
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98550.048.59721
GTATCAA172350.033.6265751
TCAACGC213400.026.3158974
ATCAACG215450.026.1430193
CAACGCA219850.025.5058525
TATCAAC229300.024.8378932
AACGCAG227650.024.6686236
ACGCAGA262050.021.4781077
CGCAGAG266950.021.0682268
GTACATG227350.020.6247771
TACATGG232300.019.5561852
ACATGGG232650.018.897993
AGAGTAC284300.017.90264310-11
GCAGAGT316400.017.6303239
GAGTACT192200.017.30319212-13
CATGGGG167100.016.991154
CAGAGTA309000.016.62022610-11
AGTACTT219850.015.2029812-13
GTACTTT222500.014.92917714-15
ATGGGGG98850.014.3190215
Produced by FastQC (version 0.11.5)