##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2139799_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9206548 Sequences flagged as poor quality 0 Sequence length 19-101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.684570481791873 31.0 31.0 34.0 30.0 34.0 2 31.867803980384394 31.0 31.0 34.0 30.0 34.0 3 31.88826659025728 31.0 31.0 34.0 30.0 34.0 4 35.63274780080438 37.0 35.0 37.0 33.0 37.0 5 35.43156555529825 37.0 35.0 37.0 33.0 37.0 6 35.43547701049297 37.0 35.0 37.0 33.0 37.0 7 35.32512566056246 37.0 35.0 37.0 32.0 37.0 8 35.30581549132204 37.0 35.0 37.0 32.0 37.0 9 36.81956385824524 39.0 37.0 39.0 32.0 39.0 10-11 36.69417321237015 39.0 35.0 39.0 32.0 39.0 12-13 36.636607607976416 39.0 35.0 39.0 32.0 39.0 14-15 37.71615387222225 39.0 37.0 41.0 32.0 41.0 16-17 37.693683126400906 39.0 37.0 41.0 32.0 41.0 18-19 37.722752871108696 39.5 37.0 41.0 32.0 41.0 20-21 37.699665675901045 39.0 37.0 41.0 32.0 41.0 22-23 37.625504369554726 39.0 36.5 41.0 32.0 41.0 24-25 37.53655174399765 39.0 36.0 41.0 32.0 41.0 26-27 37.33416927932113 39.0 36.0 40.5 31.5 41.0 28-29 37.218486402578066 39.0 36.0 40.0 31.0 41.0 30-31 37.064451721442424 39.0 36.0 40.0 31.0 41.0 32-33 37.24584467109751 39.0 36.0 40.0 31.0 41.0 34-35 37.20014923527188 39.0 36.0 40.0 31.0 41.0 36-37 37.105096519817536 39.0 36.0 40.0 31.0 41.0 38-39 36.99160060215967 39.0 35.5 40.0 31.0 41.0 40-41 36.892038799889086 39.0 35.0 40.0 30.0 41.0 42-43 36.83860519766884 39.0 35.0 40.0 30.0 41.0 44-45 36.867488105122575 39.0 35.0 40.0 30.5 41.0 46-47 36.98164275111676 39.0 35.0 40.0 31.0 41.0 48-49 36.92793010467062 39.0 35.0 40.0 30.0 41.0 50-51 36.817195384250184 39.0 35.0 40.0 30.0 41.0 52-53 36.717566808632846 39.0 35.0 40.0 30.0 41.0 54-55 36.58750628568802 38.5 35.0 40.0 30.0 41.0 56-57 36.453647344801496 38.0 35.0 40.0 30.0 41.0 58-59 36.30636268228008 38.0 34.5 40.0 29.5 41.0 60-61 36.090964451111105 38.0 34.0 40.0 29.0 41.0 62-63 35.874897873297314 38.0 34.0 40.0 29.0 41.0 64-65 35.67109594061897 37.0 34.0 40.0 28.0 41.0 66-67 35.415996936446156 37.0 34.0 39.5 28.0 41.0 68-69 35.139181726322064 36.5 33.5 39.0 28.0 41.0 70-71 34.84390176610506 36.0 33.0 39.0 28.0 40.0 72-73 34.539168915604805 35.5 33.0 38.5 28.0 40.0 74-75 34.18877074678997 35.0 32.5 38.0 28.0 40.0 76-77 33.1560551490323 34.0 31.0 36.5 26.0 39.0 78-79 33.577753310420995 35.0 32.0 37.0 27.0 39.0 80-81 33.47942328273378 35.0 32.0 36.0 27.0 39.0 82-83 33.25095878524378 35.0 32.0 36.0 27.0 37.5 84-85 33.017579350500284 34.5 32.0 35.5 27.0 37.0 86-87 32.801456255031425 34.0 32.0 35.0 27.0 37.0 88-89 32.5945230354678 34.0 32.0 35.0 26.5 36.0 90-91 32.470324491342566 34.0 31.0 35.0 26.0 36.0 92-93 32.39053579843247 34.0 31.0 35.0 26.5 36.0 94-95 32.355994731730846 34.0 31.0 35.0 27.0 35.0 96-97 32.373492554775865 34.0 31.0 35.0 27.0 35.0 98-99 32.51058435461495 34.0 31.5 35.0 27.5 35.0 100-101 31.88593600167021 33.5 30.5 34.5 27.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 18.0 20 81.0 21 614.0 22 2618.0 23 9092.0 24 23139.0 25 46791.0 26 82209.0 27 127081.0 28 178591.0 29 235208.0 30 293649.0 31 359077.0 32 438910.0 33 542395.0 34 687026.0 35 890857.0 36 1226162.0 37 1699336.0 38 1665517.0 39 680080.0 40 18094.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.610812217565154 23.203941368686724 16.036412344778956 24.148834068969173 2 13.732719274434643 23.65360699861171 40.73112894748076 21.882544779472887 3 16.370717884705538 28.47115987447195 32.2975669056415 22.860555335181004 4 11.968731385531255 20.37508521109106 39.85593731765695 27.800246085720726 5 12.128910857793821 38.832687343833975 36.728217785862846 12.310184012509358 6 27.770397764721373 40.54689119092194 19.345709162652494 12.337001881704197 7 24.219848742438536 33.59532802088253 25.256002575558178 16.92882066112076 8 23.203561204481854 37.91510129529548 22.09565409315196 16.785683407070707 9 24.905273941981292 16.584761193880702 22.130042660940887 36.37992220319712 10-11 23.05422727389245 27.531296203528182 30.346194903887973 19.068281618691394 12-13 24.042339213351195 25.24343000221147 30.129332948679572 20.58489783575777 14-15 21.279658781988644 26.557304648821688 27.610967759034118 24.552068810155557 16-17 20.82370069650427 29.49518103853909 28.322157229832506 21.35896103512413 18-19 20.886445906490156 28.80035983247283 29.8624510828293 20.450743178207716 20-21 21.66283376034533 28.024851766921564 29.638713253618516 20.67360121911459 22-23 21.37692788921766 27.96701955726631 29.50570781718667 21.15034473632936 24-25 21.228647052514365 28.124373458333952 29.48335421760275 21.16362527154893 26-27 21.234645171755336 28.245137791740106 29.38789752300588 21.132319513498683 28-29 21.197277470087617 28.275810643816442 29.448173885735784 21.078738000360158 30-31 21.447856324707676 28.17936562166905 29.307594280076465 21.06518377354681 32-33 21.264273451124353 28.301619526458875 29.245702817014884 21.188404205401888 34-35 21.396837507545825 28.316688016505502 29.240960518761927 21.04551395718674 36-37 21.369902992355243 28.2727392423324 29.216306417247583 21.141051348064767 38-39 21.23909857649824 28.369067650753106 29.256140507562513 21.135693265186145 40-41 21.446668023532702 28.240081153086177 29.137649149258387 21.175601674122735 42-43 21.358748723136955 28.379693269465534 28.88933073876487 21.372227268632642 44-45 21.407651197712102 28.42370384143406 28.74954587292206 21.419099087931773 46-47 21.466333844080783 28.399585626126555 28.552224620245738 21.581855909546924 48-49 21.399529578954198 28.54123717707704 28.52748075129803 21.53175249267073 50-51 21.46483293741212 28.611215208434466 28.359835545555185 21.564116308598226 52-53 21.55866094082982 28.499690852794224 28.273392616242216 21.66825559013374 54-55 21.534777093876702 28.41600122268867 28.352446685748923 21.696774997685704 56-57 21.545864902428995 28.454369853061124 28.31680915522334 21.68295608928654 58-59 21.52386464234966 28.493504995960468 28.276341944440997 21.706288417248878 60-61 21.627463423755277 28.453174235079757 28.29896382037979 21.620398520785173 62-63 21.55510013430976 28.508405766902754 28.28974679321929 21.64674730556819 64-65 21.502294385356162 28.488144627995272 28.247271881521712 21.76228910512685 66-67 21.597034167933298 28.603180620366487 28.03246905824957 21.767316153450643 68-69 21.538796562653772 28.61427443367857 28.05217068526516 21.7947583184025 70-71 21.544217377678137 28.398434344978902 28.166577664440435 21.89077061290253 72-73 21.580732166695913 28.4313801473443 28.074767390245775 21.913120295714016 74-75 21.537977486738882 28.4802457432959 28.04520516160315 21.93657160836207 76-77 21.67690913745907 28.41276086833776 27.99327238274841 21.917057611454766 78-79 21.670833997401598 28.401963550220216 27.953345984841686 21.973856467536496 80-81 21.65013062090502 28.422398318345376 27.94601330280035 21.98145775794925 82-83 21.671365810252716 28.3907259796774 28.043841321663308 21.894066888406574 84-85 21.743853422040008 28.39182728557853 28.0256406108885 21.838678681492958 86-87 21.6157162395634 28.427778507280355 28.093940095871396 21.862565157284855 88-89 21.658352939902574 28.40119804290483 28.05845749156176 21.881991525630834 90-91 21.68929562270872 28.39344443290112 28.155287401223138 21.761972543167015 92-93 21.750472717238264 28.277851119940046 28.170654842725483 21.801021320096208 94-95 21.553452640658787 28.306901713332138 28.262084923357023 21.877560722652053 96-97 21.4249804699446 28.348018376899486 28.380091924278585 21.84690922887733 98-99 21.534591067561166 28.921815728817553 28.502270224223007 21.04132297939828 100-101 22.064378191712926 31.71365540156794 25.75863893275589 20.46332747396324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2179.0 1 1995.5 2 2744.0 3 3867.0 4 5158.0 5 7789.5 6 9955.5 7 11589.0 8 12564.5 9 12573.0 10 11930.0 11 11269.5 12 12628.5 13 16830.0 14 26490.0 15 39811.5 16 50050.0 17 54770.0 18 55793.5 19 54974.0 20 53145.0 21 51940.0 22 53879.5 23 60972.5 24 73208.5 25 90809.0 26 113134.0 27 136167.0 28 157826.0 29 179330.5 30 200656.0 31 222280.0 32 244747.5 33 266883.0 34 286850.0 35 306201.0 36 326721.5 37 343695.0 38 355742.5 39 365460.5 40 374164.5 41 381348.0 42 384477.0 43 386181.5 44 388811.0 45 388681.5 46 384350.5 47 377742.0 48 369629.0 49 357961.0 50 343222.0 51 326455.0 52 306397.5 53 285080.0 54 263195.0 55 240951.0 56 218716.5 57 194814.0 58 170422.0 59 146178.5 60 121114.5 61 97403.5 62 76559.5 63 58680.0 64 44081.5 65 32704.0 66 24258.5 67 18420.0 68 14049.5 69 10219.0 70 7354.0 71 5255.5 72 3640.5 73 2460.0 74 1659.0 75 1166.0 76 816.5 77 547.5 78 381.5 79 272.5 80 186.0 81 130.5 82 105.5 83 80.0 84 61.5 85 51.0 86 39.5 87 29.0 88 19.5 89 12.0 90 7.0 91 4.5 92 4.0 93 3.0 94 2.0 95 1.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0028783861225727602 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.5076612862931907E-4 20-21 6.354493398061543E-4 22-23 3.0418857344956306E-4 24-25 1.5213563108848154E-4 26-27 0.002451537426523863 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.03211896287170051 38-39 0.015383217723771252 40-41 0.02106269319554524 42-43 0.009524606320020475 44-45 0.003813926040141912 46-47 0.004899722362453347 48-49 0.008843462935394125 50-51 0.0066407046736330815 52-53 0.010836558056268916 54-55 0.003415447933910308 56-57 0.01654451470496809 58-59 0.012861365607030006 60-61 0.01263406557221435 62-63 0.004624726448052201 64-65 0.006310732947632733 66-67 3.308535646576623E-4 68-69 3.545610540597305E-4 70-71 7.776198650378646E-4 72-73 1.0598677563255392E-4 74-75 0.0024377634950356257 76-77 0.002915016044507119 78-79 0.0024742122122138727 80-81 4.7748516987237104E-4 82-83 0.002990480138358596 84-85 0.004366428029044435 86-87 0.0013794785379327458 88-89 0.07461330135004991 90-91 9.353836226748337E-4 92-93 1.5678844582041986E-5 94-95 4.901937936965579E-4 96-97 0.011195480970209828 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution warn #Length Count 18-19 282.0 20-21 998.0 22-23 2063.0 24-25 3750.0 26-27 4833.0 28-29 8026.0 30-31 20546.0 32-33 27220.0 34-35 39172.0 36-37 57404.0 38-39 62115.0 40-41 68700.0 42-43 79037.0 44-45 93404.0 46-47 94661.0 48-49 85986.0 50-51 78649.0 52-53 71988.0 54-55 78781.0 56-57 84270.0 58-59 89564.0 60-61 90023.0 62-63 87321.0 64-65 94397.0 66-67 101290.0 68-69 104304.0 70-71 104376.0 72-73 102358.0 74-75 104134.0 76-77 104521.0 78-79 112669.0 80-81 115804.0 82-83 112354.0 84-85 113928.0 86-87 121688.0 88-89 129846.0 90-91 141849.0 92-93 150085.0 94-95 179370.0 96-97 239662.0 98-99 763366.0 100-101 5081754.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 87.34735746123192 #Duplication Level Percentage of deduplicated Percentage of total 1 91.33095925466532 79.77517945294458 2 6.159704520669213 10.7606782524492 3 1.4135544631363943 3.7041074094748327 4 0.5170790766970322 1.8066196379191772 5 0.22076617527431647 0.9641671013517351 6 0.11567536610193696 0.6062362531418853 7 0.07125263948162089 0.4356610839600199 8 0.0437106975728615 0.3054411140621229 9 0.028498799653343242 0.2240365356482944 >10 0.09713045608203827 1.2916057560665666 >50 0.0014081545467552463 0.0793078922312285 >100 2.6039611920709903E-4 0.04695951075029162 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0861834424802869E-5 2 0.0 3.2585503274408605E-5 0.0 0.0 1.0861834424802869E-5 3 0.0 3.2585503274408605E-5 0.0 1.0861834424802869E-5 1.0861834424802869E-5 4 0.0 4.3447337699211476E-5 0.0 1.0861834424802869E-5 1.0861834424802869E-5 5 0.0 5.430917212401434E-5 0.0 2.1723668849605738E-5 1.0861834424802869E-5 6 1.0861834424802869E-5 5.430917212401434E-5 0.0 2.1723668849605738E-5 1.0861834424802869E-5 7 2.1723668849605738E-5 5.430917212401434E-5 0.0 2.1723668849605738E-5 1.0861834424802869E-5 8 2.1723668849605738E-5 6.517100654881721E-5 0.0 2.1723668849605738E-5 2.1723668849605738E-5 9 2.1723668849605738E-5 1.1948017867283155E-4 0.0 4.3447337699211476E-5 2.1723668849605738E-5 10-11 2.715458606200717E-5 1.1948017867283155E-4 0.0 9.232559261082438E-5 2.1723668849605738E-5 12-13 3.2585503274408605E-5 1.1948017867283155E-4 0.0 1.0861834424802868E-4 2.1723668849605738E-5 14-15 3.2585503274408605E-5 1.5206568194724016E-4 0.0 1.1948017867283155E-4 2.715458606200717E-5 16-17 1.4663476473483875E-4 1.5206568194724016E-4 0.0 1.6292751637204305E-4 3.801642048681004E-5 18-19 1.5206568194724016E-4 1.737893507968459E-4 0.0 2.063748540712545E-4 4.3447337699211476E-5 20-21 1.5206568194724016E-4 1.9551301964645164E-4 0.0 2.6611494340767026E-4 4.3447337699211476E-5 22-23 1.6292751637204302E-4 2.2809852292086024E-4 0.0 4.344733769921147E-4 4.887825491161291E-5 24-25 1.737893507968459E-4 2.7697677783247314E-4 0.0 9.667032638074552E-4 7.603284097362008E-5 26-27 1.737893507968459E-4 2.9326952946967744E-4 0.0 0.0024819291660674553 7.603284097362008E-5 28-29 1.737893507968459E-4 2.9326952946967744E-4 0.0 0.014299605020252976 7.603284097362008E-5 30-31 1.8465118522164876E-4 3.2585503274408604E-4 0.0 0.02503652834917061 8.146375818602152E-5 32-33 1.9551301964645164E-4 3.2585503274408604E-4 0.0 0.03911889668092754 8.689467539842295E-5 34-35 2.0094393685885306E-4 3.367168671688889E-4 0.0 0.057518844196543586 8.689467539842295E-5 36-37 2.1723668849605736E-4 3.475787015936918E-4 0.0 0.08123022874588826 8.689467539842295E-5 38-39 2.226676057084588E-4 3.475787015936918E-4 0.0 0.12299398210925529 8.689467539842295E-5 40-41 2.2809852292086024E-4 3.475787015936918E-4 0.0 0.19096191102245924 8.689467539842295E-5 42-43 2.2809852292086024E-4 3.638714532308961E-4 0.0 0.22649097142598942 8.689467539842295E-5 44-45 2.2809852292086024E-4 4.181806253549104E-4 0.0 0.274999923967159 8.689467539842295E-5 46-47 2.2809852292086024E-4 4.561970458417205E-4 0.0 0.3275874953348421 8.689467539842295E-5 48-49 2.3352944013326166E-4 4.670588802665233E-4 0.0 0.3705514814021499 8.689467539842295E-5 50-51 2.6068402619526884E-4 5.539535556649464E-4 0.0 0.4108923344558677 8.689467539842295E-5 52-53 2.6068402619526884E-4 5.648153900897492E-4 0.0 0.4611228877533686 8.689467539842295E-5 54-55 2.6611494340767026E-4 5.811081417269535E-4 0.0 0.5548713806738421 8.689467539842295E-5 56-57 2.769767778324732E-4 6.191245622137635E-4 0.0 0.6509931844161352 8.689467539842295E-5 58-59 2.9870044668207885E-4 6.299863966385664E-4 0.0 0.7302302665450721 9.775650982322582E-5 60-61 3.1499319831928315E-4 6.408482310633692E-4 0.0 0.7742532814687981 1.0861834424802868E-4 62-63 3.367168671688889E-4 6.62571899912975E-4 0.0 0.7992572243146943 1.0861834424802868E-4 64-65 3.367168671688889E-4 7.38604740886595E-4 0.0 0.8153055846773405 1.0861834424802868E-4 66-67 3.421477843812904E-4 7.440356580989965E-4 0.0 0.8232781711451458 1.0861834424802868E-4 68-69 3.475787015936918E-4 7.548974925237993E-4 0.0 0.8309194716629946 1.0861834424802868E-4 70-71 3.6387145323089605E-4 7.766211613734051E-4 0.0 0.8386639596078791 1.0861834424802868E-4 72-73 3.801642048681004E-4 7.820520785858066E-4 0.0 0.8447900342234679 1.1948017867283155E-4 74-75 3.801642048681004E-4 7.983448302230109E-4 0.0 0.8496887215490541 1.1948017867283155E-4 76-77 3.910260392929033E-4 8.417921679222223E-4 0.0 0.8540986263255239 1.1948017867283155E-4 78-79 4.018878737177061E-4 8.526540023470251E-4 0.0 0.8581500905659755 1.1948017867283155E-4 80-81 4.018878737177061E-4 8.580849195594266E-4 0.0 0.860322457450936 1.1948017867283155E-4 82-83 4.073187909301076E-4 8.580849195594266E-4 0.0 0.8615607065753635 1.1948017867283155E-4 84-85 4.12749708142509E-4 8.580849195594266E-4 0.0 0.8629292977128886 1.1948017867283155E-4 86-87 4.12749708142509E-4 8.580849195594266E-4 0.0 0.8633366165038188 1.1948017867283155E-4 88-89 4.236115425673119E-4 8.743776711966309E-4 0.0 0.8633963565931552 1.1948017867283155E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9855 0.0 48.5972 1 GTATCAA 17235 0.0 33.626575 1 TCAACGC 21340 0.0 26.315897 4 ATCAACG 21545 0.0 26.143019 3 CAACGCA 21985 0.0 25.505852 5 TATCAAC 22930 0.0 24.837893 2 AACGCAG 22765 0.0 24.668623 6 ACGCAGA 26205 0.0 21.478107 7 CGCAGAG 26695 0.0 21.068226 8 GTACATG 22735 0.0 20.624777 1 TACATGG 23230 0.0 19.556185 2 ACATGGG 23265 0.0 18.89799 3 AGAGTAC 28430 0.0 17.902643 10-11 GCAGAGT 31640 0.0 17.630323 9 GAGTACT 19220 0.0 17.303192 12-13 CATGGGG 16710 0.0 16.99115 4 CAGAGTA 30900 0.0 16.620226 10-11 AGTACTT 21985 0.0 15.20298 12-13 GTACTTT 22250 0.0 14.929177 14-15 ATGGGGG 9885 0.0 14.319021 5 >>END_MODULE