FastQCFastQC Report
Fri 27 May 2016
SRR2139798_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139798_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8703956
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98650.049.9874231
GTATCAA186750.037.0803071
TCAACGC237650.028.3077744
ATCAACG239850.028.1181133
CAACGCA244150.027.6057035
TATCAAC250650.027.091362
GACCGAG68600.026.78815390-91
AACGCAG252150.026.7797856
AGACCGA68800.026.48159690-91
ACCGAGG71350.026.37941792-93
GAGACCG70600.025.99742988-89
CGAGACC72350.025.66457488-89
ACGAGAC77450.024.14126286-87
CACGAGA78200.023.48958886-87
ACGCAGA290300.023.1882177
CCCACGA79300.022.91788384-85
CGCAGAG294350.022.8691678
GCCCACG79550.022.73292482-83
CCACGAG81300.022.64161184-85
ATCTCCG82050.021.51288474-75
Produced by FastQC (version 0.11.5)