##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139798_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8703956
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74445964570593	31.0	31.0	34.0	30.0	34.0
2	31.919773836172887	31.0	31.0	34.0	30.0	34.0
3	31.940937890770588	31.0	31.0	34.0	30.0	34.0
4	35.676381636120404	37.0	35.0	37.0	33.0	37.0
5	35.47868796671307	37.0	35.0	37.0	33.0	37.0
6	35.48602842201868	37.0	35.0	37.0	33.0	37.0
7	35.37394731774839	37.0	35.0	37.0	33.0	37.0
8	35.35542332704807	37.0	35.0	37.0	33.0	37.0
9	36.8845628355658	39.0	37.0	39.0	33.0	39.0
10-11	36.76070398333815	39.0	36.0	39.0	32.0	39.0
12-13	36.70365773907864	39.0	35.0	39.0	32.0	39.0
14-15	37.79604360362116	40.0	37.0	41.0	32.0	41.0
16-17	37.774319516321086	39.0	37.0	41.0	32.0	41.0
18-19	37.8056776711647	39.5	37.0	41.0	32.0	41.0
20-21	37.78381782733719	40.0	37.0	41.0	32.0	41.0
22-23	37.710036105539245	39.0	37.0	41.0	32.0	41.0
24-25	37.621483443460974	39.0	36.5	41.0	32.0	41.0
26-27	37.42237511301757	39.0	36.0	41.0	32.0	41.0
28-29	37.30884732681231	39.0	36.0	40.5	31.0	41.0
30-31	37.153522981258504	39.0	36.0	40.0	31.0	41.0
32-33	37.33425199836523	39.0	36.0	40.5	31.5	41.0
34-35	37.28715818605947	39.0	36.0	41.0	31.0	41.0
36-37	37.19253388745766	39.0	36.0	40.5	31.0	41.0
38-39	37.08344129140716	39.0	36.0	40.0	31.0	41.0
40-41	36.98285595990138	39.0	35.0	40.0	31.0	41.0
42-43	36.93406315324229	39.0	35.0	40.0	30.5	41.0
44-45	36.96014980980871	39.0	35.5	40.0	30.5	41.0
46-47	37.06424883293571	39.0	36.0	40.0	31.0	41.0
48-49	37.0054771231959	39.0	35.0	40.0	31.0	41.0
50-51	36.8936777053449	39.0	35.0	40.0	30.0	41.0
52-53	36.7889863366274	39.0	35.0	40.0	30.0	41.0
54-55	36.65087727432434	39.0	35.0	40.0	30.0	41.0
56-57	36.51509976122413	38.0	35.0	40.0	30.0	41.0
58-59	36.36306746695017	38.0	35.0	40.0	30.0	41.0
60-61	36.14263656520287	38.0	34.0	40.0	29.0	41.0
62-63	35.917880350130986	38.0	34.0	40.0	29.0	41.0
64-65	35.71093767577137	37.0	34.0	40.0	29.0	41.0
66-67	35.455557937960684	37.0	34.0	39.5	28.5	41.0
68-69	35.17224347871719	36.0	34.0	39.0	28.0	41.0
70-71	34.872962589692534	36.0	33.0	39.0	28.0	40.0
72-73	34.56677908367264	35.5	33.0	38.5	28.0	40.0
74-75	34.221142350513816	35.0	33.0	38.0	28.0	40.0
76-77	33.18728440579831	34.5	31.5	36.5	26.5	39.0
78-79	33.60981944047961	35.0	32.0	37.0	27.5	39.0
80-81	33.50645712488917	35.0	32.0	36.0	28.0	39.0
82-83	33.27367406233218	35.0	32.0	36.0	27.0	37.5
84-85	33.05771549694916	34.5	32.0	35.5	27.0	37.0
86-87	32.857668451878766	34.0	32.0	35.0	27.0	37.0
88-89	32.65719390631589	34.0	32.0	35.0	27.0	36.0
90-91	32.53135137030157	34.0	32.0	35.0	27.0	36.0
92-93	32.464584487254356	34.0	32.0	35.0	27.0	36.0
94-95	32.42534469371102	34.0	32.0	35.0	27.0	35.0
96-97	32.43801199276075	34.0	31.5	35.0	27.0	35.0
98-99	32.575250118703416	34.0	32.0	35.0	28.0	35.0
100-101	31.960303800276392	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	0.0
17	0.0
18	2.0
19	11.0
20	80.0
21	463.0
22	2276.0
23	7765.0
24	20164.0
25	41815.0
26	74191.0
27	115697.0
28	163685.0
29	215513.0
30	271175.0
31	332703.0
32	408025.0
33	505690.0
34	644721.0
35	837789.0
36	1161383.0
37	1628586.0
38	1601415.0
39	650861.0
40	19945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.228784244773294	22.756319080657118	15.865774137644998	24.14912253692459
2	13.836359190996694	23.208590237105177	40.78878189926798	22.166268672630142
3	16.51992496285597	28.207920628275236	31.993521106954127	23.27863330191467
4	12.051795758158704	19.86808067504018	39.516031560821304	28.56409200597981
5	12.308451997793187	38.747309556093704	36.351766105381536	12.59247234073158
6	28.24930410953364	39.94672077845981	19.174327168014177	12.629647943992364
7	24.643529907550086	33.43421083470551	24.863923944468468	17.05833531327594
8	23.638998175082687	37.514229162004035	21.857153230094454	16.989619432818824
9	25.213397218460205	16.463938926161852	21.780245672197793	36.54241818318015
10-11	23.39597075169038	27.328475695419417	29.973083503639035	19.30247004925117
12-13	24.325450404390832	25.02012303371019	29.833463082763746	20.820963479135234
14-15	21.571099394344365	26.305325991997204	27.336041220796613	24.78753339286182
16-17	21.085349006819428	29.222614406598563	28.026307807622192	21.66572877895982
18-19	21.147076325786333	28.514084947591716	29.62739947215734	20.71143925446461
20-21	22.007146681967825	27.709544211599514	29.385689941591753	20.89761916484091
22-23	21.72501706291096	27.624029795614508	29.235628127996854	21.415325013477673
24-25	21.486360090171246	27.846076703031137	29.24583446478422	21.421728742013393
26-27	21.456242940589846	27.943112473634567	29.18214765907156	21.418496926704027
28-29	21.44737051772431	27.94495635242758	29.23690424697849	21.370768882869623
30-31	21.695328185817413	27.867624444057913	29.088237699583352	21.348809670541318
32-33	21.530329756161766	28.044124510855777	29.009640438404848	21.415905294577612
34-35	21.65668626262405	28.03171096967645	29.009180743967644	21.30242202373186
36-37	21.62449861055198	27.972617627874936	28.988098768228593	21.41478499334449
38-39	21.49840588886852	28.113718141939444	29.033651765061464	21.354224204130574
40-41	21.684400864009458	27.966310873896273	28.926192281679867	21.423095980414402
42-43	21.54990336364872	28.10425445407133	28.6725126919347	21.67332949034525
44-45	21.65344361057027	28.16336411149136	28.52184350342471	21.661348774513655
46-47	21.72812746341364	28.098176015263636	28.336415754716736	21.83728076660599
48-49	21.664525238989945	28.262795694076203	28.261699044579352	21.810980022354496
50-51	21.709417992297958	28.337683721425062	28.124269351289094	21.828628934987883
52-53	21.82355901272743	28.22928369796135	28.002237266017495	21.944920023293726
54-55	21.801098422759665	28.12060687690706	28.06760070716007	22.0106939931732
56-57	21.755946334235844	28.19559280629417	28.08499302882786	21.96346783064213
58-59	21.750482587857938	28.24366251541121	28.061316969993094	21.944537926737755
60-61	21.77669658152332	28.066617210375906	28.134079327752627	22.02260688034815
62-63	21.918231431698647	28.142761245002568	28.128583580854983	21.810423742443803
64-65	21.647958243430125	28.123120544277512	28.07717728522516	22.151743927067205
66-67	21.749759324012395	28.23439588140817	27.90951584222443	22.106328952355
68-69	21.65956372248588	28.418805860258544	27.94559326176414	21.976037155491433
70-71	21.714694756895046	28.19177329933949	28.046354521758765	22.047177422006705
72-73	21.732569812823456	28.21152245544509	27.736093294452544	22.31981443727891
74-75	21.624469183509802	28.281068496381963	27.73900113349158	22.355461186616655
76-77	21.797074828409315	27.98240888047297	27.914967321388932	22.305548969728783
78-79	21.7731649393824	27.944790518167935	27.862756443231522	22.419288099218146
80-81	22.04559741179835	27.976525851921817	27.591673549296708	22.386203186983124
82-83	22.008265648407818	28.192276387726135	27.73914056225139	22.060317401614657
84-85	21.818211832494168	27.943306213222186	27.701852084773826	22.536629869509817
86-87	21.727868624895407	28.222299990781657	27.752626112414863	22.297205271908073
88-89	22.035391912244872	27.888400850148177	27.7387918858668	22.33741535174015
90-91	22.083468268127092	28.077415524431657	27.841311572582782	21.997804634858465
92-93	21.85153702699654	27.99958791507951	27.797942006143916	22.350933051780036
94-95	21.918490236434348	27.767649541798495	27.937034821176027	22.376825400591134
96-97	21.817635925671024	28.083215760495527	28.030617687543014	22.068530626290432
98-99	22.021934920674234	28.522636723096646	28.17232068746244	21.283107668766675
100-101	22.339074285082937	31.36038304447874	25.479882059142867	20.820660611295455
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2383.0
1	2115.5
2	2740.5
3	3865.0
4	5141.5
5	7707.0
6	9768.0
7	11435.0
8	12745.5
9	12818.5
10	11881.0
11	11043.5
12	12365.0
13	16547.5
14	26149.0
15	39132.5
16	48886.5
17	53276.5
18	53869.0
19	52264.0
20	49584.0
21	47684.5
22	48779.5
23	54689.5
24	65445.0
25	81078.0
26	100461.5
27	119947.0
28	138972.5
29	158069.5
30	175650.5
31	193507.0
32	214091.0
33	234341.5
34	252183.0
35	270636.5
36	291545.0
37	308682.5
38	320595.0
39	329495.0
40	337477.5
41	345466.5
42	350317.0
43	355104.0
44	358668.5
45	359142.5
46	356660.0
47	352004.0
48	347629.5
49	341054.0
50	331719.5
51	319094.0
52	302857.5
53	285083.0
54	266726.0
55	247091.0
56	226046.5
57	204502.5
58	181467.0
59	156591.5
60	130751.5
61	105870.5
62	83623.5
63	64372.5
64	47812.5
65	35131.5
66	26225.5
67	19677.0
68	14674.0
69	10517.5
70	7385.5
71	5230.5
72	3635.0
73	2419.5
74	1633.5
75	1155.5
76	820.0
77	567.0
78	401.0
79	292.0
80	211.0
81	135.0
82	94.5
83	73.5
84	54.0
85	39.0
86	28.0
87	21.0
88	14.0
89	11.0
90	9.5
91	8.5
92	6.5
93	3.5
94	1.0
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0035386208294251485
3	0.0
4	0.0
5	2.2978057333929538E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.193495463442141E-4
20-21	6.032033891528176E-4
22-23	2.1258569358036022E-4
24-25	1.379290606703387E-4
26-27	0.0024032680305615204
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.031877851566622734
38-39	0.016134732926246117
40-41	0.021169328862103758
42-43	0.009692709608559537
44-45	0.0034846283805768374
46-47	0.005407358044272562
48-49	0.010101633211719634
50-51	0.007805347739374615
52-53	0.012192025017985421
54-55	0.003171585214296273
56-57	0.017778949913858796
58-59	0.014319187126948126
60-61	0.013849545383023222
62-63	0.005241283429091735
64-65	0.006742904593903953
66-67	4.339191820850391E-4
68-69	4.124164113065379E-4
70-71	0.0010412881721172623
72-73	1.5270571976626308E-4
74-75	0.0025106802368112984
76-77	0.0032575614094876586
78-79	0.002739695329976062
80-81	4.110476694918364E-4
82-83	0.002915290384917495
84-85	0.004475098914072952
86-87	0.0013234843526445602
88-89	0.07432993233955812
90-91	9.022185874434646E-4
92-93	8.15686632772168E-6
94-95	5.346254339028375E-4
96-97	0.011225511932805203
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	261.0
20-21	934.0
22-23	1851.0
24-25	3373.0
26-27	4436.0
28-29	7512.0
30-31	18778.0
32-33	25248.0
34-35	35660.0
36-37	53419.0
38-39	57990.0
40-41	63674.0
42-43	73502.0
44-45	86497.0
46-47	88297.0
48-49	79776.0
50-51	73041.0
52-53	66374.0
54-55	72528.0
56-57	76693.0
58-59	48671.0
60-61	84774.0
62-63	81251.0
64-65	86587.0
66-67	93326.0
68-69	96685.0
70-71	96287.0
72-73	93453.0
74-75	95298.0
76-77	95399.0
78-79	104061.0
80-81	106364.0
82-83	103183.0
84-85	103732.0
86-87	110934.0
88-89	119696.0
90-91	131070.0
92-93	139015.0
94-95	164461.0
96-97	221501.0
98-99	757345.0
100-101	4881019.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.6358093867718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.29278881314306	80.00517438815417
2	6.303263745152485	11.04783240169465
3	1.4137784195892111	3.716928482827548
4	0.4747442736436444	1.6641839468998343
5	0.1991513227010559	0.8726393677676606
6	0.10638385242390527	0.5593821007711095
7	0.06159103215052277	0.3778305967434423
8	0.03914272673797566	0.27442436314301843
9	0.0260454091700505	0.2054259462084322
>10	0.0810325463908814	1.0985633575284555
>50	0.0017037024626063392	0.09836393163007459
>100	3.354008712186616E-4	0.05668220858310951
>500	3.875556336366245E-5	0.022568908048448733
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1489028666964769E-5
2	0.0	2.2978057333929538E-5	0.0	0.0	1.1489028666964769E-5
3	0.0	2.2978057333929538E-5	0.0	0.0	1.1489028666964769E-5
4	0.0	5.7445143334823844E-5	0.0	0.0	2.2978057333929538E-5
5	0.0	5.7445143334823844E-5	0.0	0.0	2.2978057333929538E-5
6	4.5956114667859075E-5	5.7445143334823844E-5	0.0	0.0	2.2978057333929538E-5
7	4.5956114667859075E-5	6.893417200178861E-5	0.0	0.0	3.446708600089431E-5
8	4.5956114667859075E-5	9.191222933571815E-5	0.0	0.0	3.446708600089431E-5
9	5.7445143334823844E-5	1.6084640133750676E-4	0.0	0.0	3.446708600089431E-5
10-11	8.042320066875338E-5	1.6084640133750676E-4	0.0	0.0	3.446708600089431E-5
12-13	9.191222933571815E-5	1.6084640133750676E-4	0.0	0.0	4.021160033437669E-5
14-15	1.091457723361653E-4	2.182915446723306E-4	0.0	0.0	6.318965766830623E-5
16-17	1.895689730049187E-4	2.182915446723306E-4	0.0	5.7445143334823844E-6	6.893417200178861E-5
18-19	2.0680251600536584E-4	2.2978057333929538E-4	0.0	3.446708600089431E-5	9.765674366920054E-5
20-21	2.182915446723306E-4	2.4126960200626015E-4	0.0	7.4678686335271E-5	1.0340125800268292E-4
22-23	2.2403605900581298E-4	2.929702310076016E-4	0.0	2.0105800167188344E-4	1.2063480100313007E-4
24-25	2.5850314500670726E-4	3.1594828834153113E-4	0.0	5.22750804346897E-4	1.49357372670542E-4
26-27	2.8148120234063685E-4	3.2169280267501353E-4	0.0	0.001596974984708103	1.49357372670542E-4
28-29	2.872257166741192E-4	3.2169280267501353E-4	0.0	0.013063025594338942	1.49357372670542E-4
30-31	2.872257166741192E-4	3.906269746768021E-4	0.0	0.021139812747215173	1.49357372670542E-4
32-33	2.929702310076016E-4	4.021160033437669E-4	0.0	0.02823428794906592	1.49357372670542E-4
34-35	3.1594828834153113E-4	4.4232760367814356E-4	0.0	0.03729338705296764	1.49357372670542E-4
36-37	3.676489173428726E-4	4.825392040125203E-4	0.0	0.0498279173286262	1.49357372670542E-4
38-39	3.7913794600983735E-4	4.997727470129674E-4	0.0	0.07460400764893572	1.6659091567098916E-4
40-41	3.9062697467680214E-4	5.859404620152032E-4	0.0	0.12306473056619313	1.7233543000447153E-4
42-43	3.9062697467680214E-4	5.97429490682168E-4	0.0	0.14391731759673418	1.7233543000447153E-4
44-45	3.963714890102845E-4	6.433856053500271E-4	0.0	0.17515024202787788	1.780799443379539E-4
46-47	4.078605176772493E-4	6.778526913509213E-4	0.0	0.210984522440141	2.0105800167188344E-4
48-49	4.136050320107317E-4	6.893417200178861E-4	0.0	0.24085599697424942	2.2978057333929538E-4
50-51	4.250940606776965E-4	7.812539493536043E-4	0.0	0.26971643698566494	2.2978057333929538E-4
52-53	4.365830893446612E-4	7.812539493536043E-4	0.0	0.3066479196356232	2.7573668800715445E-4
54-55	4.4807211801162596E-4	7.984874923540515E-4	0.0	0.38222849472125087	3.3892634567546067E-4
56-57	4.5956114667859075E-4	8.272100640214634E-4	0.0	0.46687391342511386	3.73393431676355E-4
58-59	4.710501753455555E-4	8.329545783549458E-4	0.0	0.5369914553795998	3.9062697467680214E-4
60-61	4.825392040125203E-4	8.50188121355393E-4	0.0	0.9548933841117764	4.078605176772493E-4
62-63	5.22750804346897E-4	8.789106930228048E-4	0.0	0.9653943563133821	4.4807211801162596E-4
64-65	5.284953186803793E-4	9.70822922358523E-4	0.0	0.9756368253699812	4.5381663234510836E-4
66-67	5.629624046812737E-4	9.823119510254876E-4	0.0	0.9836044667505213	4.6530566101207315E-4
68-69	5.801959476817208E-4	0.0010052900083594172	0.0	0.9916812539033975	4.710501753455555E-4
70-71	5.859404620152032E-4	0.0010225235513598645	0.0	0.9994306037392652	4.710501753455555E-4
72-73	6.089185193491327E-4	0.0010225235513598645	0.0	1.005134906472413	5.284953186803793E-4
74-75	6.204075480160975E-4	0.0010397570943603115	0.0	1.0104715602882184	5.284953186803793E-4
76-77	6.204075480160975E-4	0.0011029467520286178	0.0	1.014659311237327	5.457288616808265E-4
78-79	6.318965766830623E-4	0.0011144357806955826	0.0	1.0185540919554281	5.629624046812737E-4
80-81	6.318965766830623E-4	0.0011144357806955826	0.0	1.020805941574153	5.68706919014756E-4
82-83	6.318965766830623E-4	0.0011144357806955826	0.0	1.0221444134138546	6.089185193491328E-4
84-85	6.318965766830623E-4	0.0011374138380295122	0.0	1.0234541626818885	6.089185193491328E-4
86-87	6.433856053500271E-4	0.0011603918953634416	0.0	1.0239539354289016	6.089185193491328E-4
88-89	6.433856053500271E-4	0.0012063480100313008	0.0	1.0239826580005689	6.089185193491328E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9865	0.0	49.987423	1
GTATCAA	18675	0.0	37.080307	1
TCAACGC	23765	0.0	28.307774	4
ATCAACG	23985	0.0	28.118113	3
CAACGCA	24415	0.0	27.605703	5
TATCAAC	25065	0.0	27.09136	2
GACCGAG	6860	0.0	26.788153	90-91
AACGCAG	25215	0.0	26.779785	6
AGACCGA	6880	0.0	26.481596	90-91
ACCGAGG	7135	0.0	26.379417	92-93
GAGACCG	7060	0.0	25.997429	88-89
CGAGACC	7235	0.0	25.664574	88-89
ACGAGAC	7745	0.0	24.141262	86-87
CACGAGA	7820	0.0	23.489588	86-87
ACGCAGA	29030	0.0	23.188217	7
CCCACGA	7930	0.0	22.917883	84-85
CGCAGAG	29435	0.0	22.869167	8
GCCCACG	7955	0.0	22.732924	82-83
CCACGAG	8130	0.0	22.641611	84-85
ATCTCCG	8205	0.0	21.512884	74-75
>>END_MODULE