FastQCFastQC Report
Fri 27 May 2016
SRR2139797_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139797_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9052839
Sequences flagged as poor quality0
Sequence length19-101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96500.050.2756161
TATCTCG91500.036.74226894-95
GTATCAA173700.035.380771
CCGTCTT36250.031.34035792-93
TATGCCG34800.031.17749888-89
TGCCGTC38950.028.31175890-91
GCCGTCT38600.027.93361790-91
TCAACGC219900.027.4996764
ATCAACG222000.027.2203063
CAACGCA226250.026.7467355
ATGCCGT39350.026.7392488-89
AACGCAG231650.026.1048076
TATCAAC233900.026.0190222
CGTATGC44200.025.38980386-87
CTCGTAT44250.024.91215184-85
TCGTATG43950.024.49738184-85
CTATCTC155800.024.48547794-95
TCTCGTA43750.024.27345882-83
ATCTCGT73350.022.69104894-95
ACGCAGA270700.022.3232677
Produced by FastQC (version 0.11.5)