##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139797_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9052839
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.81760826631292	31.0	31.0	34.0	30.0	34.0
2	31.990171149624995	31.0	31.0	34.0	30.0	34.0
3	32.014249783962796	31.0	31.0	34.0	30.0	34.0
4	35.73145186830341	37.0	35.0	37.0	35.0	37.0
5	35.5373293394481	37.0	35.0	37.0	33.0	37.0
6	35.54043477410788	37.0	35.0	37.0	33.0	37.0
7	35.431088965572016	37.0	35.0	37.0	33.0	37.0
8	35.41271064248464	37.0	35.0	37.0	33.0	37.0
9	36.957262025757885	39.0	37.0	39.0	33.0	39.0
10-11	36.84031081299469	39.0	37.0	39.0	32.5	39.0
12-13	36.782623826625	39.0	37.0	39.0	32.0	39.0
14-15	37.90104220344579	40.0	37.0	41.0	33.0	41.0
16-17	37.877838598477226	40.0	37.0	41.0	33.0	41.0
18-19	37.905410059761365	40.0	37.0	41.0	33.0	41.0
20-21	37.87879123421117	40.0	37.0	41.0	32.0	41.0
22-23	37.805493310412444	40.0	37.0	41.0	32.0	41.0
24-25	37.71819184929513	39.0	37.0	41.0	32.0	41.0
26-27	37.52314303895761	39.0	36.0	41.0	32.0	41.0
28-29	37.41228526691806	39.0	36.0	41.0	32.0	41.0
30-31	37.260566707878795	39.0	36.0	40.0	31.0	41.0
32-33	37.44531731307862	39.0	36.0	41.0	32.0	41.0
34-35	37.4025787945916	39.0	36.0	41.0	31.5	41.0
36-37	37.31003190468193	39.0	36.0	41.0	31.0	41.0
38-39	37.202218403220726	39.0	36.0	41.0	31.0	41.0
40-41	37.105640752427476	39.0	36.0	40.0	31.0	41.0
42-43	37.0564403483137	39.0	36.0	40.0	31.0	41.0
44-45	37.07555987793925	39.0	35.5	40.0	31.0	41.0
46-47	37.18113830275095	39.0	36.0	41.0	31.0	41.0
48-49	37.11786844257601	39.0	36.0	41.0	31.0	41.0
50-51	36.999805551474964	39.0	35.0	40.5	31.0	41.0
52-53	36.89219690707664	39.0	35.0	40.0	30.5	41.0
54-55	36.76388162862594	39.0	35.0	40.0	30.0	41.0
56-57	36.6274896405599	38.5	35.0	40.0	30.0	41.0
58-59	36.48216878290255	38.0	35.0	40.0	30.0	41.0
60-61	36.25820832725422	38.0	34.5	40.0	29.5	41.0
62-63	36.036812334688335	38.0	34.0	40.0	29.0	41.0
64-65	35.84010662295144	37.0	34.0	40.0	29.0	41.0
66-67	35.57834821145393	37.0	34.0	40.0	29.0	41.0
68-69	35.294714434392404	36.5	34.0	39.0	29.0	41.0
70-71	34.990559908362144	36.0	33.5	39.0	28.0	40.0
72-73	34.68519583791881	35.5	33.0	39.0	28.0	40.0
74-75	34.321869373583795	35.0	33.0	38.0	28.0	40.0
76-77	33.257858757368396	34.5	31.5	36.5	26.5	39.0
78-79	33.639543533299204	35.0	32.0	37.0	27.5	39.0
80-81	33.51739652444624	35.0	32.0	36.0	28.0	39.0
82-83	33.28821885344847	35.0	32.0	36.0	27.0	37.5
84-85	33.0871661932736	35.0	32.0	35.5	27.0	37.0
86-87	32.89419505175593	34.0	32.0	35.0	27.0	37.0
88-89	32.68814508855313	34.0	32.0	35.0	27.0	36.0
90-91	32.58812066920021	34.0	32.0	35.0	27.0	36.0
92-93	32.53491941437786	34.0	32.0	35.0	27.0	36.0
94-95	32.4935800323167	34.0	32.0	35.0	27.0	35.0
96-97	32.49684539158197	34.0	32.0	35.0	27.0	35.0
98-99	32.61993114349782	34.0	32.0	35.0	28.5	35.0
100-101	31.9985853877526	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	2.0
13	0.0
14	0.0
15	0.0
16	2.0
17	0.0
18	2.0
19	21.0
20	79.0
21	474.0
22	2252.0
23	7798.0
24	20196.0
25	42499.0
26	75048.0
27	117905.0
28	166293.0
29	218534.0
30	274858.0
31	338407.0
32	413454.0
33	511752.0
34	652868.0
35	855245.0
36	1200638.0
37	1720949.0
38	1744494.0
39	665582.0
40	23485.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.78901845045515	22.28299873663941	15.508295243072368	24.419687569833066
2	13.950137884805983	22.893296469786637	40.53833179931608	22.618233846091304
3	16.655592792493053	27.7338191919684	31.733967653683003	23.876620361855547
4	12.144775799061486	19.5364570164122	39.33535104291593	28.983416141610384
5	12.562806299110314	38.42852967369114	36.26497514479996	12.743688882398596
6	28.694501249828917	39.521944441959036	19.036028366350045	12.747525941861996
7	24.9773689778422	33.13982497645214	24.62889266008155	17.253913385624113
8	23.83397075768165	37.12400054833627	21.814902485286662	17.227126208695413
9	25.317604786741487	16.36080129117507	21.608226988240926	36.71336693384252
10-11	23.53609735023455	27.058406760575327	29.879350555113156	19.526145334076965
12-13	24.46120493250791	24.78306529034704	29.678993517945035	21.076736259200015
14-15	21.75690962801835	26.064851037337565	27.08820956608198	25.090029768562104
16-17	21.35569294891912	29.02965577980565	27.776656582537257	21.837994688737975
18-19	21.388670632507544	28.317348791960594	29.344046693131382	20.94993388240048
20-21	22.1844137318429	27.55487762206186	29.07248413436229	21.188224511732955
22-23	21.940891069801925	27.446762907464212	28.968004989363354	21.64434103337051
24-25	21.750727560285878	27.65328053726713	28.95835535487078	21.637636547576218
26-27	21.7650631558354	27.710506230120156	28.87645671254878	21.64797390149566
28-29	21.718454322620424	27.740083602567346	28.913918824161495	21.627543250650735
30-31	21.94511698204147	27.65119525941887	28.78676936995494	21.616918388584722
32-33	21.76406336396764	27.818168609354082	28.705657535167255	21.712110491511023
34-35	21.89419136788601	27.808923040215788	28.685275664845193	21.611609927053006
36-37	21.86048985848979	27.76395427805448	28.6881451837958	21.68741067965993
38-39	21.71755209716701	27.87526504391513	28.757844399526412	21.649338459391444
40-41	21.886705739500872	27.638829533454984	28.67529412030725	21.799170606736897
42-43	21.83964139331778	27.82161403753304	28.46169517133834	21.87704939781084
44-45	21.835378554707166	27.825483246455274	28.315035344036595	22.02410285480097
46-47	21.898789607663034	27.829092284306157	28.145507745180964	22.126610362849846
48-49	21.790441946709628	28.093305890263743	28.09187172626457	22.024380436762062
50-51	21.840144253340497	28.11637990445236	27.984907115120983	22.058568727086158
52-53	21.928543371489013	27.950700763321656	27.74553796629442	22.375217898894913
54-55	21.871317563716534	27.693689671770123	27.94859034567271	22.486402418840637
56-57	21.843430591294464	27.485956951957718	28.20446859354221	22.46614386320561
58-59	21.742182036734842	27.309945848347393	28.269044510810065	22.678827604107695
60-61	21.85009693625266	27.075375971200543	28.538659358692104	22.535867733854694
62-63	21.56869925869084	27.5628486018395	28.55338698608106	22.3150651533886
64-65	21.24267588850201	27.784142685526238	28.349913851642487	22.62326757432927
66-67	21.317089937526372	28.358155128998835	27.85144956655244	22.473305366922354
68-69	21.378867111143453	28.62792048599697	27.461827607579025	22.531384795280548
70-71	21.39261611710338	28.26852460978734	27.387571826437057	22.951287446672218
72-73	21.325176476403072	27.885549921748385	27.330529172425006	23.458744429423533
74-75	21.67191626323561	27.591913467681195	27.439527066254055	23.29664320282914
76-77	22.12163609944914	27.379256083393617	27.338453407499042	23.160654409658203
78-79	22.28587075919765	27.296646105302198	26.722349389082307	23.695133746417845
80-81	22.229414774900327	27.69096380288959	26.625235025758954	23.45438639645113
82-83	22.107684036269852	27.77770378571094	26.731050346065498	23.38356183195371
84-85	22.11616000665806	27.793226924421717	26.711089219598318	23.37952384932191
86-87	22.4043799457161	28.085472478346183	26.71164396629841	22.798503609639305
88-89	22.57300690897602	27.76612431896817	26.973949937146667	22.68691883490914
90-91	22.300702910713976	27.77746221244478	27.280756640752767	22.641078236088475
92-93	22.126860931399285	27.2871768152254	27.173655453366667	23.412306800008643
94-95	21.890207757210405	27.26655018592632	27.807875828407102	23.035366228456176
96-97	21.5193267166127	27.561936003758085	28.089211381021702	22.829525898607514
98-99	21.70880248181343	27.763536070315826	28.395400217188705	22.132261230682047
100-101	22.356160559370643	30.681855177988815	25.749649825277825	21.212334437362713
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2057.0
1	1827.5
2	2459.5
3	3569.0
4	4801.5
5	7323.5
6	9393.5
7	11065.0
8	12212.5
9	12161.0
10	11511.5
11	10888.5
12	11987.0
13	15622.0
14	24116.0
15	36001.5
16	45587.5
17	50196.5
18	50720.5
19	48886.5
20	46197.5
21	44605.5
22	45185.5
23	49487.5
24	59093.0
25	73549.0
26	90554.0
27	108381.0
28	127198.0
29	146181.0
30	162641.0
31	180378.0
32	200619.0
33	220583.5
34	239944.0
35	257632.5
36	277104.5
37	296009.5
38	311847.0
39	324661.5
40	335443.5
41	347038.5
42	354799.0
43	360781.5
44	367394.5
45	372090.0
46	373604.5
47	371829.5
48	368693.0
49	362689.5
50	354115.0
51	343354.5
52	328170.5
53	310669.5
54	292745.0
55	271850.0
56	247621.0
57	221828.5
58	195382.5
59	168102.5
60	140543.0
61	114317.0
62	90936.5
63	70090.0
64	51659.5
65	37960.5
66	28321.0
67	21412.0
68	16442.0
69	12061.5
70	8632.5
71	6257.0
72	4411.0
73	3015.0
74	2111.0
75	1473.0
76	1023.5
77	716.5
78	518.0
79	386.0
80	273.0
81	192.5
82	137.5
83	94.0
84	71.0
85	53.0
86	42.0
87	39.5
88	33.5
89	22.5
90	14.0
91	9.5
92	6.5
93	3.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003921421777190559
3	0.0
4	0.0
5	5.5231292636486746E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.3138775581892046E-4
20-21	7.62227496075495E-4
22-23	2.7067996298202926E-4
24-25	1.3813804720762778E-4
26-27	0.002493080457952899
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03291906495316792
38-39	0.01653015504250185
40-41	0.02118257548920559
42-43	0.009974472864480732
44-45	0.004013172852349686
46-47	0.00495679069890401
48-49	0.008604243601237677
50-51	0.006613654290826708
52-53	0.010551610871333598
54-55	0.0034779629951883807
56-57	0.01590618097725762
58-59	0.012301061118939903
60-61	0.012019934728693931
62-63	0.004474466348648114
64-65	0.005979571797717213
66-67	3.7798445058720816E-4
68-69	5.896768658599302E-4
70-71	9.59392108828358E-4
72-73	1.029710096706511E-4
74-75	0.0029402004942926862
76-77	0.003547071354261274
78-79	0.0028393257056812562
80-81	3.8062082655210885E-4
82-83	0.003036266198566427
84-85	0.004350246850239145
86-87	0.0013805986745541077
88-89	0.07238231123423713
90-91	9.073445558330785E-4
92-93	0.0
94-95	4.546797216774415E-4
96-97	0.011425518582563327
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	248.0
20-21	920.0
22-23	1823.0
24-25	3711.0
26-27	4588.0
28-29	7773.0
30-31	19008.0
32-33	25016.0
34-35	35879.0
36-37	51925.0
38-39	40529.0
40-41	62467.0
42-43	70137.0
44-45	82584.0
46-47	84132.0
48-49	76717.0
50-51	70538.0
52-53	15301.0
54-55	15312.0
56-57	15223.0
58-59	15716.0
60-61	85327.0
62-63	84977.0
64-65	89910.0
66-67	97536.0
68-69	100674.0
70-71	101110.0
72-73	99327.0
74-75	100278.0
76-77	100557.0
78-79	109366.0
80-81	111311.0
82-83	108080.0
84-85	110282.0
86-87	117340.0
88-89	125868.0
90-91	136359.0
92-93	145894.0
94-95	173548.0
96-97	234012.0
98-99	842343.0
100-101	5279193.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.49902813852117
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.09821863170384	77.03310130022679
2	6.914250647107788	11.82323421267714
3	1.6577420499611577	4.252060025281164
4	0.6002016811883036	2.0526664171482594
5	0.2831469554545429	1.210439475587228
6	0.15201868297235419	0.7798469791820545
7	0.0836681529862082	0.5007482036526136
8	0.05315072286449522	0.3635468119819374
9	0.03685348756731628	0.28358436334685533
>10	0.11861389379707044	1.5472407033460516
>50	0.0017704074360657984	0.1004248673702406
>100	3.6468696086640237E-4	0.05310664019959776
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.1046258527297349E-5	0.0	0.0	0.0
3	0.0	1.1046258527297349E-5	0.0	0.0	0.0
4	0.0	3.3138775581892045E-5	0.0	1.1046258527297349E-5	0.0
5	0.0	3.3138775581892045E-5	0.0	3.3138775581892045E-5	0.0
6	2.2092517054594698E-5	3.3138775581892045E-5	0.0	3.3138775581892045E-5	1.1046258527297349E-5
7	2.2092517054594698E-5	3.3138775581892045E-5	0.0	3.3138775581892045E-5	1.1046258527297349E-5
8	2.2092517054594698E-5	3.3138775581892045E-5	0.0	3.3138775581892045E-5	1.1046258527297349E-5
9	2.2092517054594698E-5	8.837006821837879E-5	0.0	3.3138775581892045E-5	2.2092517054594698E-5
10-11	2.2092517054594698E-5	8.837006821837879E-5	0.0	3.3138775581892045E-5	2.2092517054594698E-5
12-13	3.3138775581892045E-5	8.837006821837879E-5	0.0	3.3138775581892045E-5	2.2092517054594698E-5
14-15	3.3138775581892045E-5	1.3255510232756818E-4	0.0	3.3138775581892045E-5	2.761564631824337E-5
16-17	8.837006821837879E-5	1.491244901185142E-4	0.0	4.418503410918939E-5	3.3138775581892045E-5
18-19	8.837006821837879E-5	1.8226326570040623E-4	0.0	1.2150884380027083E-4	3.866190484554072E-5
20-21	8.837006821837879E-5	2.098789120186496E-4	0.0	1.7674013643675755E-4	4.4185034109189395E-5
22-23	9.389319748202747E-5	2.4301768760054166E-4	0.0	3.700496606644612E-4	5.523129263648674E-5
24-25	1.1598571453662215E-4	2.9272585097337973E-4	0.0	9.665476211385179E-4	6.627755116378409E-5
26-27	1.2150884380027083E-4	3.203414972916231E-4	0.0	0.0028057496659335264	6.627755116378409E-5
28-29	1.2150884380027083E-4	3.258646265552718E-4	0.0	0.016105444932799532	6.627755116378409E-5
30-31	1.3255510232756818E-4	4.197578240372992E-4	0.0	0.029631588499475135	6.627755116378409E-5
32-33	1.4360136085486553E-4	4.3080408256459656E-4	0.0	0.049796533441056445	6.627755116378409E-5
34-35	1.4360136085486553E-4	4.915585044647319E-4	0.0	0.07724648588139035	6.627755116378409E-5
36-37	1.4360136085486553E-4	5.688823141558135E-4	0.0	0.11190964514004945	7.732380969108144E-5
38-39	1.4360136085486553E-4	5.854517019467595E-4	0.0	0.1796121636538549	8.837006821837879E-5
40-41	1.4360136085486553E-4	5.964979604740568E-4	0.0	0.45122309145230577	9.941632674567614E-5
42-43	1.4360136085486553E-4	6.020210897377054E-4	0.0	0.5098400623274091	9.941632674567614E-5
44-45	1.491244901185142E-4	6.351598653195976E-4	0.0	0.6020431822547601	1.049394560093248E-4
46-47	1.5464761938216288E-4	6.627755116378409E-4	0.0	0.7079602321437507	1.2150884380027083E-4
48-49	1.5464761938216288E-4	6.627755116378409E-4	0.0	0.7955791547822733	1.2150884380027083E-4
50-51	1.8778639496405493E-4	7.400993213289224E-4	0.0	0.8795307195897332	1.2150884380027083E-4
52-53	2.098789120186496E-4	7.621918383835171E-4	0.0	1.101085526871736	1.3255510232756818E-4
54-55	2.098789120186496E-4	7.842843554381118E-4	0.0	1.787897697064976	1.4360136085486553E-4
56-57	2.098789120186496E-4	8.063768724927065E-4	0.0	2.553453121170055	1.4360136085486553E-4
58-59	2.1540204128229828E-4	8.063768724927065E-4	0.0	3.3563283297096085	1.4360136085486553E-4
60-61	2.2092517054594696E-4	8.174231310200038E-4	0.0	3.9702959480445856	1.4360136085486553E-4
62-63	2.4301768760054166E-4	8.284693895473012E-4	0.0	3.9887652923022268	1.6569387790946023E-4
64-65	2.4301768760054166E-4	9.499782333475719E-4	0.0	3.998994127698505	1.6569387790946023E-4
66-67	2.4854081686419033E-4	9.499782333475719E-4	0.0	4.006704416150558	1.712170071731089E-4
68-69	2.54063946127839E-4	9.665476211385179E-4	0.0	4.013133338613445	1.7674013643675758E-4
70-71	2.595870753914877E-4	9.886401381931126E-4	0.0	4.01965063114455	2.098789120186496E-4
72-73	2.761564631824337E-4	0.0010052095259840588	0.0	4.024671155645207	2.319714290732443E-4
74-75	2.761564631824337E-4	0.0010438714308295995	0.0	4.029249829804772	2.319714290732443E-4
76-77	2.982489802370284E-4	0.0010935795942024376	0.0	4.033602055664527	2.319714290732443E-4
78-79	3.203414972916231E-4	0.0011156721112570323	0.0	4.037738879482999	2.319714290732443E-4
80-81	3.203414972916231E-4	0.0011156721112570323	0.0	4.040053070644468	2.4301768760054166E-4
82-83	3.203414972916231E-4	0.0011211952405206808	0.0	4.041527746157863	2.54063946127839E-4
84-85	3.258646265552718E-4	0.0011267183697843295	0.0	4.043063176093157	2.595870753914877E-4
86-87	3.3138775581892046E-4	0.0011377646283116268	0.0	4.043471887658667	2.6511020465513636E-4
88-89	3.3138775581892046E-4	0.0012261346965300056	0.0	4.043493980175722	2.6511020465513636E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9650	0.0	50.275616	1
TATCTCG	9150	0.0	36.742268	94-95
GTATCAA	17370	0.0	35.38077	1
CCGTCTT	3625	0.0	31.340357	92-93
TATGCCG	3480	0.0	31.177498	88-89
TGCCGTC	3895	0.0	28.311758	90-91
GCCGTCT	3860	0.0	27.933617	90-91
TCAACGC	21990	0.0	27.499676	4
ATCAACG	22200	0.0	27.220306	3
CAACGCA	22625	0.0	26.746735	5
ATGCCGT	3935	0.0	26.73924	88-89
AACGCAG	23165	0.0	26.104807	6
TATCAAC	23390	0.0	26.019022	2
CGTATGC	4420	0.0	25.389803	86-87
CTCGTAT	4425	0.0	24.912151	84-85
TCGTATG	4395	0.0	24.497381	84-85
CTATCTC	15580	0.0	24.485477	94-95
TCTCGTA	4375	0.0	24.273458	82-83
ATCTCGT	7335	0.0	22.691048	94-95
ACGCAGA	27070	0.0	22.323267	7
>>END_MODULE