FastQCFastQC Report
Fri 27 May 2016
SRR2139796_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139796_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12505391
Sequences flagged as poor quality0
Sequence length19-101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145250.051.3837171
GTATCAA257700.033.704691
TCAACGC353150.026.4016253
ATCAACG355550.026.2126462
CAACGCA362200.025.7883324
TATCAAC369850.025.2105061
AACGCAG373100.025.102475
ACGCAGA431250.021.7955516
CGCAGAG437350.021.5011587
AGAGTAC462750.018.84160210-11
CAGAGTA499350.018.7558489
GCAGAGT511350.018.4388988
AGTACTT357700.016.9252212-13
GAGTACT320150.016.92290112-13
TGGTATC65550.016.0825632
GTACATG306700.015.9264941
GTGGTAT70450.015.798621
TACATGG308900.015.6906932
ACATGGG311500.015.0063133
GTACTTT364050.014.91753114-15
Produced by FastQC (version 0.11.5)