##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139796_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12505391
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.307735679756036	31.0	31.0	34.0	30.0	34.0
2	31.526447513716285	31.0	31.0	34.0	30.0	34.0
3	31.52459703179213	31.0	31.0	34.0	30.0	34.0
4	35.32860715830476	37.0	35.0	37.0	33.0	37.0
5	35.07459438893194	35.0	35.0	37.0	32.0	37.0
6	35.101172846174904	36.0	35.0	37.0	32.0	37.0
7	34.99159898319053	36.0	35.0	37.0	32.0	37.0
8	34.971143805099736	36.0	35.0	37.0	32.0	37.0
9	36.447123404618054	38.0	35.0	39.0	32.0	39.0
10-11	36.265949421333566	38.0	35.0	39.0	32.0	39.0
12-13	36.20989579614104	38.0	35.0	39.0	32.0	39.0
14-15	37.18560795100289	39.0	36.0	40.0	32.0	41.0
16-17	37.15041916722156	39.0	36.0	40.0	31.5	41.0
18-19	37.17254798350567	39.0	36.0	40.0	31.0	41.0
20-21	37.14674118286913	39.0	36.0	40.0	31.0	41.0
22-23	37.06075554055735	39.0	36.0	40.0	31.0	41.0
24-25	36.95515644374881	39.0	36.0	40.0	31.0	41.0
26-27	36.71561168563492	38.5	35.5	40.0	30.0	41.0
28-29	36.572848287625995	38.0	35.0	40.0	30.0	41.0
30-31	36.39720377297637	38.0	35.0	40.0	30.0	41.0
32-33	36.58211696151549	38.0	35.0	40.0	30.0	41.0
34-35	36.518542633638916	38.0	35.0	40.0	30.0	41.0
36-37	36.4063748466382	38.0	35.0	40.0	30.0	41.0
38-39	36.26759119059579	38.0	35.0	40.0	30.0	41.0
40-41	36.15574923015292	38.0	35.0	40.0	30.0	41.0
42-43	36.077468244529015	38.0	35.0	40.0	30.0	41.0
44-45	36.110397405834576	38.0	34.5	40.0	29.5	41.0
46-47	36.241784691352564	38.0	35.0	40.0	30.0	41.0
48-49	36.18064783821008	38.0	35.0	40.0	29.5	41.0
50-51	36.07089937012556	38.0	34.0	40.0	29.0	41.0
52-53	35.95429630852799	38.0	34.0	40.0	29.0	41.0
54-55	35.811208051018355	38.0	34.0	40.0	28.0	41.0
56-57	35.67482381555291	38.0	34.0	40.0	28.0	41.0
58-59	35.52223489800778	38.0	34.0	40.0	28.0	41.0
60-61	35.30465123716263	37.0	33.0	40.0	28.0	41.0
62-63	35.078381770340016	37.0	33.0	40.0	28.0	41.0
64-65	34.869873506232935	36.5	33.0	39.0	27.0	40.0
66-67	34.612200426869435	36.0	32.5	39.0	27.0	40.0
68-69	34.33124869976116	36.0	32.0	39.0	26.0	40.0
70-71	34.04491804416624	35.0	32.0	38.5	26.0	40.0
72-73	33.73797916693009	35.0	31.5	38.0	26.0	40.0
74-75	33.3873828904578	35.0	31.0	37.5	26.0	39.5
76-77	32.48128800900682	34.0	30.5	36.0	25.5	39.0
78-79	32.93240142806137	34.0	31.0	36.0	26.0	39.0
80-81	32.8372676286574	34.0	31.0	36.0	26.0	38.5
82-83	32.60858762611559	34.0	31.0	36.0	26.0	37.5
84-85	32.37806151621341	34.0	31.0	35.0	26.0	37.0
86-87	32.147490695500174	34.0	31.0	35.0	26.0	36.5
88-89	31.929799376704537	34.0	31.0	35.0	26.0	36.0
90-91	31.797940457357065	34.0	31.0	35.0	25.0	36.0
92-93	31.717486955953802	34.0	30.5	35.0	25.0	35.5
94-95	31.69426270538053	34.0	31.0	35.0	25.0	35.0
96-97	31.73291584322277	34.0	31.0	35.0	25.5	35.0
98-99	31.928190191453524	34.0	31.0	35.0	26.0	35.0
100-101	31.331931536897525	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	2.0
18	7.0
19	42.0
20	270.0
21	1436.0
22	6316.0
23	19583.0
24	47006.0
25	92675.0
26	155503.0
27	232591.0
28	317605.0
29	406377.0
30	499882.0
31	601118.0
32	724735.0
33	885539.0
34	1106868.0
35	1410735.0
36	1840305.0
37	2164365.0
38	1575598.0
39	413834.0
40	2998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18780142002768	25.651986863264558	18.006755487033992	23.153456229673765
2	14.132985381079369	25.660870980980338	39.073098121703964	21.133045516236333
3	16.593923372727808	29.131748059696815	32.92590371624526	21.34842485133012
4	12.706224059687537	22.141082993726467	38.69796634107642	26.454726605509578
5	11.922235132210991	39.39091063933232	36.74180493371259	11.945049294744106
6	27.551055984659417	41.7321091145498	18.74118280197579	11.975652098814992
7	23.80693444986522	33.98998351910656	25.268120386489347	16.934961644538877
8	23.169305395513454	38.554639239559904	21.800527612493493	16.475527752433152
9	24.768135979765525	16.342441139380696	21.98512001624899	36.90430286460478
10-11	23.018474433824135	27.981998162392372	30.317071278864553	18.682456124918932
12-13	23.92742857860262	25.646847027813845	30.20011129600026	20.225613097583274
14-15	21.1197571607123	26.973700940154604	27.747167421407887	24.159374477725205
16-17	20.6103528158658	29.90540878773073	28.46468602738499	21.01955236901848
18-19	20.678530403223906	29.072295944091064	30.205580258345737	20.043593394339297
20-21	21.462019378544177	28.252690963534306	30.054081378497553	20.231208279423964
22-23	21.15894148722527	28.208159978864522	29.939111123748884	20.69378741016132
24-25	21.028546359529834	28.398812583332962	29.900182076316504	20.672458980820696
26-27	21.0527603043723	28.475924087696896	29.801414423171067	20.669901184759738
28-29	21.033662430793534	28.51961183209408	29.857708007366107	20.58901772974628
30-31	21.25485653861051	28.417820570253404	29.769054033896612	20.558268857239472
32-33	21.138153637134668	28.54092555299641	29.636278493936434	20.684642315932486
34-35	21.225201246403326	28.586189338324967	29.661310981177763	20.527298434093943
36-37	21.19440978682204	28.554996195818255	29.628676624705573	20.621917392654133
38-39	21.113978043422243	28.638095344794046	29.647038693401313	20.600887918382398
40-41	21.31846898722357	28.54867986683636	29.487643633639394	20.645207512300676
42-43	21.224629115886895	28.689056321377848	29.223450660827925	20.862863901907332
44-45	21.357391991869363	28.755454080986027	29.011028461933012	20.876125465211597
46-47	21.395157136451807	28.77911137818026	28.759091018392414	21.06664046697552
48-49	21.35403626202849	28.91988526050811	28.70958737664811	21.016491100815283
50-51	21.431232553002946	28.946432509463516	28.529159614329245	21.093175323204292
52-53	21.49601638675512	28.898609657841	28.46771504099394	21.137658914409936
54-55	21.494434014339355	28.828303570134455	28.502985866184087	21.1742765493421
56-57	21.510885943466654	28.87537453985859	28.471663152683195	21.14207636399156
58-59	21.499114193468625	28.94744422343864	28.45229366526677	21.10114791782597
60-61	21.60052285323855	28.908741778690583	28.427032196136537	21.063703171934332
62-63	21.548724489622046	28.932037379134762	28.41280447339119	21.106433657852005
64-65	21.474892508229466	28.89454732245768	28.41887374041977	21.211686428893085
66-67	21.596703130118133	28.945656758831657	28.283905589213116	21.173734521837098
68-69	21.532727790171606	28.88008444595827	28.353462652720413	21.23372511114971
70-71	21.529380784178954	28.706823056792153	28.427244938331043	21.336551220697853
72-73	21.563445781709632	28.78353536923824	28.370136044338235	21.28288280471389
74-75	21.475731811536523	28.842700616384942	28.324589001425345	21.356978570653197
76-77	21.52158752869245	28.796256303628848	28.339542607344793	21.342613560333916
78-79	21.50417170347109	28.822720039983317	28.280370989812987	21.392737266732603
80-81	21.53125288704815	28.773433403910857	28.31382425538808	21.381489453652904
82-83	21.495277692438698	28.737970275350612	28.431842188509016	21.334909843701674
84-85	21.53554285882687	28.734261748727913	28.371405100548515	21.358790291896703
86-87	21.45936137782218	28.76347063625747	28.38106525614586	21.396102729774487
88-89	21.528274890363473	28.67793917831457	28.391034546916483	21.402751384405473
90-91	21.496299445365448	28.6741961455022	28.486769147565518	21.34273526156683
92-93	21.500191171365007	28.59732962886725	28.48569217334499	21.41678702642275
94-95	21.283570437109866	28.665192663357114	28.572001060118296	21.479235839414727
96-97	21.186780957354703	28.760879168863955	28.665432294170152	21.38690757961119
98-99	21.2909368274652	29.346282395347455	28.777166208596704	20.585614568590646
100-101	21.81361971950054	32.17041328383777	26.095366747118355	19.920600249543337
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4261.0
1	3729.0
2	4634.5
3	6348.5
4	7911.0
5	10885.0
6	13451.0
7	16423.0
8	19021.5
9	19740.0
10	18859.5
11	17757.0
12	19838.5
13	26866.0
14	43999.0
15	67730.5
16	85041.5
17	91122.0
18	90806.5
19	88477.5
20	85002.5
21	82337.0
22	83434.5
23	92171.0
24	109014.5
25	134147.5
26	165222.0
27	196082.5
28	226865.5
29	258134.5
30	286520.5
31	315587.0
32	346781.5
33	376237.5
34	403160.0
35	428412.0
36	455075.0
37	477624.5
38	491874.5
39	500300.5
40	508770.5
41	516887.5
42	519832.5
43	520016.0
44	518665.0
45	514186.5
46	504975.5
47	493038.0
48	479285.5
49	460405.5
50	439334.0
51	415861.0
52	387849.5
53	358755.0
54	329075.0
55	299143.5
56	270820.0
57	242057.0
58	210755.0
59	179127.5
60	148090.0
61	119472.5
62	93778.5
63	71917.0
64	53801.0
65	39758.5
66	29729.5
67	22645.5
68	17367.5
69	12793.0
70	9374.5
71	6840.5
72	4910.5
73	3542.5
74	2532.5
75	1762.0
76	1257.0
77	903.0
78	636.5
79	450.0
80	315.5
81	223.0
82	164.5
83	117.5
84	83.0
85	60.0
86	54.5
87	45.0
88	30.5
89	20.5
90	15.5
91	13.5
92	13.0
93	9.5
94	4.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.99655124737803E-6
2	0.001815217133154813
3	0.0
4	0.0
5	7.99655124737803E-6
6	7.99655124737803E-6
7	7.99655124737803E-6
8	7.99655124737803E-6
9	7.99655124737803E-6
10-11	7.99655124737803E-6
12-13	0.0
14-15	7.99655124737803E-6
16-17	7.99655124737803E-6
18-19	4.478068698531697E-4
20-21	4.6783326422463037E-4
22-23	2.479557151092815E-4
24-25	1.40021580125929E-4
26-27	0.002285614100677899
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.02896292874690328
38-39	0.012077816167898346
40-41	0.01973820595042537
42-43	0.0074019740304715155
44-45	0.0026755762522353253
46-47	0.004232143470758541
48-49	0.008340947301078296
50-51	0.006171710902983966
52-53	0.009867436615244194
54-55	0.0024534544332830218
56-57	0.015046330268800442
58-59	0.01176099119603894
60-61	0.011495692083839756
62-63	0.004175681413046956
64-65	0.005610267137782193
66-67	2.832194868378618E-4
68-69	8.928811851935674E-5
70-71	5.71117964843977E-4
72-73	8.676750689910139E-5
74-75	8.594264061204832E-4
76-77	9.451889855890351E-4
78-79	7.577102918283554E-4
80-81	1.1210588094712143E-4
82-83	0.0020090886406283603
84-85	0.00321003974134447
86-87	9.154646063180977E-4
88-89	0.06317495074738111
90-91	6.074760235065355E-4
92-93	0.0
94-95	3.461742465136146E-4
96-97	0.009578415987689062
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	557.0
20-21	1848.0
22-23	3490.0
24-25	6347.0
26-27	8571.0
28-29	12741.0
30-31	25182.0
32-33	33735.0
34-35	48366.0
36-37	70629.0
38-39	78609.0
40-41	86331.0
42-43	102455.0
44-45	121559.0
46-47	121929.0
48-49	108160.0
50-51	98064.0
52-53	97428.0
54-55	106515.0
56-57	112327.0
58-59	114663.0
60-61	113804.0
62-63	112254.0
64-65	119329.0
66-67	127862.0
68-69	133258.0
70-71	134264.0
72-73	132158.0
74-75	135842.0
76-77	135992.0
78-79	150094.0
80-81	156451.0
82-83	154267.0
84-85	159082.0
86-87	171531.0
88-89	187519.0
90-91	208620.0
92-93	226506.0
94-95	278988.0
96-97	375575.0
98-99	1081714.0
100-101	6850775.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.92429387543316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.10922592179128	79.19605128789019
2	6.295759429338431	10.945088856096865
3	1.4467792767848204	3.772808010843911
4	0.5362827408565521	1.8646399426615081
5	0.24365661628296437	1.0589839659237024
6	0.11651839194130387	0.607696736579928
7	0.07553592521323314	0.45961348729714735
8	0.045130535271663504	0.3138351928567744
9	0.02900194270893151	0.22688760518906748
>10	0.0998787926791068	1.3513198854383428
>50	0.00165153211936542	0.09783075307296323
>100	5.505440339910617E-4	0.08973100539954333
>500	2.8350978367237978E-5	0.015513270750050864
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.7979307484268186E-5	0.0	0.0	0.0
3	0.0	4.7979307484268186E-5	0.0	0.0	0.0
4	0.0	9.595861496853637E-5	0.0	7.99655124737803E-6	0.0
5	0.0	1.039551662159144E-4	0.0	7.99655124737803E-6	0.0
6	7.99655124737803E-6	1.039551662159144E-4	0.0	7.99655124737803E-6	1.599310249475606E-5
7	7.99655124737803E-6	1.039551662159144E-4	0.0	2.3989653742134093E-5	1.599310249475606E-5
8	7.99655124737803E-6	1.1195171746329243E-4	0.0	2.3989653742134093E-5	1.599310249475606E-5
9	1.599310249475606E-5	1.7592412744231667E-4	0.0	4.7979307484268186E-5	3.998275623689015E-5
10-11	1.599310249475606E-5	1.7592412744231667E-4	0.0	7.99655124737803E-5	4.7979307484268186E-5
12-13	2.3989653742134093E-5	1.7592412744231667E-4	0.0	8.796206372115834E-5	5.5975858731646215E-5
14-15	3.598448061320114E-5	2.318999861739629E-4	0.0	9.595861496853637E-5	6.397240997902424E-5
16-17	4.7979307484268186E-5	2.318999861739629E-4	0.0	1.1195171746329243E-4	7.196896122640228E-5
18-19	4.7979307484268186E-5	2.798792936582311E-4	0.0	1.2394654433435948E-4	7.59672368500913E-5
20-21	4.7979307484268186E-5	3.358551523898773E-4	0.0	1.3594137120542652E-4	8.396378809746932E-5
22-23	6.397240997902424E-5	4.0382583799259054E-4	0.0	1.519344737001826E-4	8.796206372115834E-5
24-25	7.59672368500913E-5	4.358120429821027E-4	0.0	1.799224030660057E-4	9.196033934484735E-5
26-27	7.99655124737803E-5	4.558034211005478E-4	0.0	2.2390343492658486E-4	9.595861496853637E-5
28-29	7.99655124737803E-5	4.598016967242368E-4	0.0	8.556309834694492E-4	9.595861496853637E-5
30-31	7.99655124737803E-5	4.7979307484268186E-4	0.0	0.0022710205542553607	9.995689059222538E-5
32-33	8.396378809746932E-5	4.8778962609005987E-4	0.0	0.0056495634562725786	1.1994826871067047E-4
34-35	9.595861496853637E-5	5.31770657950639E-4	0.0	0.010379523519096684	1.1994826871067047E-4
36-37	9.595861496853637E-5	5.717534141875293E-4	0.0	0.01662083176767524	1.2394654433435948E-4
38-39	9.595861496853637E-5	5.757516898112182E-4	0.0	0.025900829490257442	1.279448199580485E-4
40-41	1.039551662159144E-4	6.077378948007304E-4	0.0	0.03599247716444852	1.279448199580485E-4
42-43	1.0795344183960341E-4	6.237309972954864E-4	0.0	0.044936619734640845	1.319430955817375E-4
44-45	1.1994826871067045E-4	6.996982341455776E-4	0.0	0.05591588459729088	1.3993964682911555E-4
46-47	1.279448199580485E-4	7.636706441246019E-4	0.0	0.06842648902381382	1.519344737001826E-4
48-49	1.3594137120542652E-4	7.7166719537198E-4	0.0	0.08013743832559894	1.519344737001826E-4
50-51	1.519344737001826E-4	8.1164995160887E-4	0.0	0.09129662559131499	1.519344737001826E-4
52-53	1.519344737001826E-4	8.156482272325591E-4	0.0	0.10190804909658563	1.519344737001826E-4
54-55	1.559327493238716E-4	8.196465028562482E-4	0.0	0.11348705530278902	1.559327493238716E-4
56-57	1.6792757619493864E-4	8.476344322220713E-4	0.0	0.125649809750051	1.7192585181862764E-4
58-59	1.7592412744231667E-4	8.516327078457603E-4	0.0	0.13767662282610754	1.7592412744231667E-4
60-61	1.7592412744231667E-4	8.716240859642054E-4	0.0	0.14879182905996302	1.7592412744231667E-4
62-63	1.839206786896947E-4	8.956137397063394E-4	0.0	0.15905540258597273	1.7592412744231667E-4
64-65	1.839206786896947E-4	0.001039551662159144	0.0	0.1696268433350065	1.7592412744231667E-4
66-67	1.879189543133837E-4	0.0010435499377828331	0.0	0.1802502616671482	1.7592412744231667E-4
68-69	1.9191722993707274E-4	0.0010555447646539	0.0	0.19085368862117147	1.7592412744231667E-4
70-71	1.9191722993707274E-4	0.001071537867148656	0.0	0.19994576738944028	1.7592412744231667E-4
72-73	2.0791033243182882E-4	0.001071537867148656	0.0	0.20660689457850617	1.9991378118445078E-4
74-75	2.1590688367920682E-4	0.0010995257965144792	0.0	0.2123444200984999	1.9991378118445078E-4
76-77	2.1590688367920682E-4	0.0011634982064935035	0.0	0.21727829221813216	1.9991378118445078E-4
78-79	2.1590688367920682E-4	0.0012074792383540826	0.0	0.22152446093048989	1.9991378118445078E-4
80-81	2.1590688367920682E-4	0.0012114775139777717	0.0	0.2241473297396299	2.0391205680813978E-4
82-83	2.1990515930289586E-4	0.0012194740652251498	0.0	0.22548275379794203	2.318999861739629E-4
84-85	2.2390343492658486E-4	0.0012554585458383507	0.0	0.2266862347606724	2.318999861739629E-4
86-87	2.2390343492658486E-4	0.0012554585458383507	0.0	0.22713803990614928	2.318999861739629E-4
88-89	2.2390343492658486E-4	0.0012554585458383507	0.0	0.2272379967967415	2.318999861739629E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14525	0.0	51.383717	1
GTATCAA	25770	0.0	33.70469	1
TCAACGC	35315	0.0	26.401625	3
ATCAACG	35555	0.0	26.212646	2
CAACGCA	36220	0.0	25.788332	4
TATCAAC	36985	0.0	25.210506	1
AACGCAG	37310	0.0	25.10247	5
ACGCAGA	43125	0.0	21.795551	6
CGCAGAG	43735	0.0	21.501158	7
AGAGTAC	46275	0.0	18.841602	10-11
CAGAGTA	49935	0.0	18.755848	9
GCAGAGT	51135	0.0	18.438898	8
AGTACTT	35770	0.0	16.92522	12-13
GAGTACT	32015	0.0	16.922901	12-13
TGGTATC	6555	0.0	16.082563	2
GTACATG	30670	0.0	15.926494	1
GTGGTAT	7045	0.0	15.79862	1
TACATGG	30890	0.0	15.690693	2
ACATGGG	31150	0.0	15.006313	3
GTACTTT	36405	0.0	14.917531	14-15
>>END_MODULE