FastQCFastQC Report
Fri 27 May 2016
SRR2139795_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139795_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12222303
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA140650.050.088811
GTATCAA252500.035.5828631
TCAACGC324350.027.0391794
ATCAACG325300.026.947313
CAACGCA330850.026.5079575
TATCAAC341400.025.911322
AACGCAG339900.025.876286
ACGCAGA397950.022.101647
CGCAGAG402950.021.8169738
GTACATG319350.019.1023671
AGAGTAC427000.018.77417210-11
TACATGG318100.018.7022862
GCAGAGT471750.018.4749979
ACATGGG320850.018.0701033
GAGTACT284950.017.9451712-13
AGTACTT322350.016.82037512-13
CAGAGTA463850.016.80750710-11
CATGGGG239250.015.8103624
GTACTTT327650.015.63938514-15
GTGGTAT68100.014.6729811
Produced by FastQC (version 0.11.5)