##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139795_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12222303
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80128859511992	31.0	31.0	34.0	30.0	34.0
2	31.977385194917847	31.0	31.0	34.0	30.0	34.0
3	32.00072130432374	31.0	31.0	34.0	30.0	34.0
4	35.72275568687832	37.0	35.0	37.0	35.0	37.0
5	35.531715667661	37.0	35.0	37.0	33.0	37.0
6	35.53639563673065	37.0	35.0	37.0	33.0	37.0
7	35.42623260117181	37.0	35.0	37.0	33.0	37.0
8	35.40745749798545	37.0	35.0	37.0	33.0	37.0
9	36.944820464686565	39.0	37.0	39.0	33.0	39.0
10-11	36.82979582489487	39.0	37.0	39.0	32.5	39.0
12-13	36.77357835098672	39.0	37.0	39.0	32.0	39.0
14-15	37.8818464899782	40.0	37.0	41.0	33.0	41.0
16-17	37.864999092233276	40.0	37.0	41.0	33.0	41.0
18-19	37.8985980383566	40.0	37.0	41.0	33.0	41.0
20-21	37.87583244486481	40.0	37.0	41.0	32.0	41.0
22-23	37.804645409215254	40.0	37.0	41.0	32.0	41.0
24-25	37.71961498426526	39.0	37.0	41.0	32.0	41.0
26-27	37.52699159471553	39.0	36.0	41.0	32.0	41.0
28-29	37.41628290685571	39.0	36.0	41.0	32.0	41.0
30-31	37.26122346442836	39.0	36.0	40.0	31.0	41.0
32-33	37.432197380813335	39.0	36.0	41.0	32.0	41.0
34-35	37.3884058611319	39.0	36.0	41.0	31.5	41.0
36-37	37.29687277927674	39.0	36.0	41.0	31.0	41.0
38-39	37.192726230146846	39.0	36.0	41.0	31.0	41.0
40-41	37.098379870358315	39.0	36.0	40.0	31.0	41.0
42-43	37.05182357841619	39.0	35.5	40.0	31.0	41.0
44-45	37.07611820595528	39.0	35.5	40.0	31.0	41.0
46-47	37.18234087497293	39.0	36.0	41.0	31.0	41.0
48-49	37.12388881284857	39.0	36.0	41.0	31.0	41.0
50-51	37.01357662403367	39.0	35.0	40.5	31.0	41.0
52-53	36.91264011228385	39.0	35.0	40.0	31.0	41.0
54-55	36.785438261720586	39.0	35.0	40.0	30.0	41.0
56-57	36.656762405532284	38.5	35.0	40.0	30.0	41.0
58-59	36.51029367175184	38.0	35.0	40.0	30.0	41.0
60-61	36.288978135962125	38.0	34.5	40.0	29.5	41.0
62-63	36.068044558081596	38.0	34.0	40.0	29.0	41.0
64-65	35.86182841037366	37.5	34.0	40.0	29.0	41.0
66-67	35.59995497435263	37.0	34.0	40.0	29.0	41.0
68-69	35.31836896583975	36.5	34.0	39.0	29.0	41.0
70-71	35.02364749892499	36.0	33.5	39.0	28.0	40.0
72-73	34.719022098388024	35.5	33.0	39.0	28.0	40.0
74-75	34.36792571912892	35.0	33.0	38.0	28.0	40.0
76-77	33.328518345037466	34.5	31.5	36.5	26.5	39.0
78-79	33.74031232133386	35.0	32.0	37.0	28.0	39.0
80-81	33.64006967508914	35.0	32.0	36.0	28.0	39.0
82-83	33.41287588421429	35.0	32.0	36.0	28.0	37.5
84-85	33.187993023020155	35.0	32.0	36.0	28.0	37.0
86-87	32.98007156882012	34.0	32.0	35.0	27.0	37.0
88-89	32.77837248205638	34.0	32.0	35.0	27.0	36.0
90-91	32.656008021381254	34.0	32.0	35.0	27.0	36.0
92-93	32.57956184585807	34.0	32.0	35.0	27.0	36.0
94-95	32.543199035465705	34.0	32.0	35.0	27.0	35.5
96-97	32.55435737034561	34.0	32.0	35.0	27.0	35.0
98-99	32.676330387207074	34.0	32.0	35.0	29.0	35.0
100-101	32.04846468599759	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	0.0
15	0.0
16	0.0
17	1.0
18	1.0
19	17.0
20	114.0
21	679.0
22	3187.0
23	10401.0
24	26763.0
25	55547.0
26	99019.0
27	154446.0
28	218061.0
29	290417.0
30	365504.0
31	452423.0
32	553555.0
33	688911.0
34	878912.0
35	1149180.0
36	1601259.0
37	2289350.0
38	2352360.0
39	1000079.0
40	32116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.72513248935164	22.480992330168874	15.638460280357966	24.155414900121524
2	13.815494380965237	23.091626881364114	40.816684871766554	22.276193865904098
3	16.47915290596216	28.1526566638055	31.981910446828227	23.38627998340411
4	12.031447755795286	19.797226431058043	39.71088754713412	28.46043826601255
5	12.37620477518922	38.61429202242687	36.397025404627165	12.61247779775674
6	28.180081937094837	39.97960940749055	19.221352964330862	12.61895569108375
7	24.65317706491158	33.456051613186155	24.86546111645244	17.025310205449824
8	23.623755686632872	37.43412350356557	21.931431416812362	17.010689392989192
9	25.18960624687508	16.550358798992303	21.906681580386284	36.35335337374634
10-11	23.327858096792397	27.31112540738026	30.04623187626751	19.314784619559834
12-13	24.336162341908885	25.031215475512266	29.88849155515127	20.74413062742758
14-15	21.58353871606685	26.30836021656475	27.36613958924108	24.741961478127322
16-17	21.070390743872082	29.270056551535333	28.06798358705393	21.59156911753865
18-19	21.148016392543298	28.50803138812779	29.69934908543339	20.644603133895522
20-21	21.936339651682204	27.715889534015787	29.48995373079025	20.85781708351176
22-23	21.69127403751142	27.627754688804586	29.354902354060545	21.32606891962345
24-25	21.4880294571868	27.800735725720223	29.34650297815767	21.364731838935313
26-27	21.490820969764986	27.907838061741025	29.245543071602725	21.355797896891264
28-29	21.45075717781161	27.922354630661424	29.312476614000673	21.3144115775263
30-31	21.705067509796528	27.82748249524164	29.177653809330163	21.289796185631673
32-33	21.528322749111542	27.98282134290386	29.090119928010456	21.398735979974134
34-35	21.63066132684554	28.005906215801986	29.092510965232577	21.2709214921199
36-37	21.64006099556316	27.97725719325871	29.019386359026296	21.36329545215184
38-39	21.503288328722956	28.08986911992238	29.073661559593507	21.33318099176116
40-41	21.693126563054573	27.95902503850307	28.955559604270988	21.392288794171364
42-43	21.58199844091562	28.080941779459312	28.729286938905485	21.607772840719587
44-45	21.696055573086987	28.158605933568424	28.552505371307397	21.592833122037188
46-47	21.779685643679215	28.155649747757476	28.307886144319035	21.756778464244277
48-49	21.700900814994398	28.311448775744484	28.241857074564674	21.74579333469644
50-51	21.78552625964745	28.33891160790069	28.111365433962902	21.764196698488956
52-53	21.892570834335046	28.268837250753588	28.025024101146634	21.81356781376473
54-55	21.84787377952341	28.18593828963073	28.07020094555328	21.895986985292577
56-57	21.823008399496743	28.279509890278348	28.06892528876797	21.828556421456938
58-59	21.812628724825256	28.303966376719575	28.0225114482747	21.86089345018047
60-61	21.917213982827775	28.2676395812988	28.04308379517606	21.772062640697367
62-63	21.880000813620377	28.307938480012172	28.01770045162945	21.79436025473799
64-65	21.868464516878944	28.25699266128783	27.965995701621566	21.908547120211665
66-67	21.931173122745335	28.302431712247582	27.8540776736375	21.91231749136958
68-69	21.844096871480613	28.269686682819234	27.93749335325587	21.948723092444283
70-71	21.881546948426696	28.087574175897355	28.007213689262727	22.023665186413215
72-73	21.92337584005922	28.15508381317534	27.91568630318908	22.005854043576356
74-75	21.787993356490134	28.2357201964417	27.923490494422182	22.05279595264599
76-77	21.885160071189077	28.164308425935708	27.891303744301005	22.05922775857421
78-79	21.882887753106257	28.165540101189947	27.855419437051825	22.09615270865197
80-81	21.89873960275507	28.13031231068298	27.89208452967315	22.078863556888802
82-83	21.904441637419154	28.102643121818126	27.961138915232382	22.031776325530338
84-85	21.927027162487988	28.110676308260906	27.944115634097976	22.01818089515313
86-87	21.815376212114153	28.124721891863462	27.998598826976778	22.0613030690456
88-89	21.88296199954258	28.057919528129023	27.950472858786668	22.10864561354173
90-91	21.91826044330076	28.032045538038652	28.048930975749613	22.000763042910975
92-93	21.911491560124816	27.958221897276804	28.017073650985708	22.113212891612672
94-95	21.73913762516517	27.982175473048336	28.12405866978272	22.154628232003777
96-97	21.693817962240395	28.046599313285036	28.172644008052483	22.086938716422086
98-99	21.8111672667403	28.668199922540293	28.267870320342098	21.252762490377304
100-101	22.33152547094208	31.400985706569173	25.563818889497885	20.703669932990863
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3531.0
1	3131.0
2	4038.5
3	5667.0
4	7426.0
5	11147.0
6	14285.5
7	16939.5
8	18868.5
9	18871.0
10	17650.5
11	16487.5
12	18199.0
13	23831.5
14	37287.5
15	55674.0
16	69701.5
17	76401.5
18	77232.5
19	74623.5
20	70605.0
21	67993.5
22	69858.5
23	78537.0
24	93440.5
25	114921.5
26	141532.5
27	168391.0
28	195591.0
29	223240.5
30	249016.0
31	275632.0
32	303998.0
33	331942.0
34	358491.0
35	383229.0
36	410237.5
37	433553.5
38	449788.5
39	462898.5
40	473764.5
41	483266.5
42	489412.0
43	495941.5
44	501541.5
45	503347.0
46	500369.0
47	493470.5
48	485636.0
49	474224.0
50	460416.0
51	443876.5
52	422403.5
53	397752.0
54	371930.5
55	345167.0
56	316486.0
57	285276.0
58	252603.0
59	218368.0
60	183243.0
61	149411.5
62	118752.5
63	92344.0
64	69185.5
65	51179.5
66	38275.0
67	28955.0
68	22237.0
69	16291.5
70	11540.0
71	8227.5
72	5695.5
73	3884.0
74	2631.0
75	1799.0
76	1245.0
77	815.5
78	557.5
79	390.0
80	270.0
81	193.0
82	154.5
83	123.5
84	97.5
85	76.0
86	54.5
87	39.0
88	27.5
89	19.5
90	13.5
91	9.5
92	6.5
93	3.5
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0037063391408313144
3	0.0
4	0.0
5	1.6363528215590793E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.1499791815012274E-4
20-21	6.995746586075666E-4
22-23	2.20946623714643E-4
24-25	1.596162595926814E-4
26-27	0.002309363288887098
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03331785574272083
38-39	0.016171852890044875
40-41	0.021085297845379217
42-43	0.01029853513924132
44-45	0.004128727571040079
46-47	0.0049251034336525055
48-49	0.00871839581517001
50-51	0.006452668442854744
52-53	0.010516323936038161
54-55	0.0037019143204718957
56-57	0.01570333112746473
58-59	0.012455043360722371
60-61	0.012503583442793675
62-63	0.004647707293940695
64-65	0.0061988819617117925
66-67	3.679367141206274E-4
68-69	5.996844595786317E-4
70-71	9.891327607136054E-4
72-73	9.916215416888852E-5
74-75	0.0031526710578822876
76-77	0.004001454353244221
78-79	0.0032304802353603215
80-81	5.801985753145578E-4
82-83	0.003347310698439984
84-85	0.004704973657799944
86-87	0.001628990164944547
88-89	0.07101517574908171
90-91	0.001081154453328968
92-93	5.776008470862983E-6
94-95	4.7841495102241704E-4
96-97	0.011433011749772357
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	337.0
20-21	1295.0
22-23	2572.0
24-25	5271.0
26-27	7394.0
28-29	12252.0
30-31	27208.0
32-33	35851.0
34-35	50642.0
36-37	74593.0
38-39	84381.0
40-41	88561.0
42-43	104036.0
44-45	122546.0
46-47	124036.0
48-49	111540.0
50-51	100907.0
52-53	99403.0
54-55	108437.0
56-57	113431.0
58-59	114047.0
60-61	112914.0
62-63	109680.0
64-65	116593.0
66-67	123986.0
68-69	127476.0
70-71	127582.0
72-73	124319.0
74-75	126026.0
76-77	126703.0
78-79	137290.0
80-81	141026.0
82-83	137494.0
84-85	139656.0
86-87	148234.0
88-89	158790.0
90-91	174964.0
92-93	183750.0
94-95	218806.0
96-97	300173.0
98-99	1016791.0
100-101	6981310.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.15019652319108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.06936201191508	76.69423876033011
2	6.800925622413893	11.582003065762974
3	1.7169760828544676	4.386025526420302
4	0.6323123948004008	2.153660987252149
5	0.29304550933442464	1.2476441355032442
6	0.168337454991954	0.8600380424867229
7	0.09209437270633491	0.5489297754237096
8	0.05740097854596571	0.3910163683049963
9	0.038490875903355246	0.2949755082768414
>10	0.12879440430675823	1.6703035832470237
>50	0.001896203236864446	0.1111473958140018
>100	3.4819440756446375E-4	0.052843688847626885
>500	1.589458286706169E-5	0.007173162330357579
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.909058464677238E-5	0.0	0.0	0.0
3	0.0	4.909058464677238E-5	0.0	0.0	0.0
4	0.0	8.181764107795397E-5	0.0	0.0	0.0
5	0.0	8.999940518574936E-5	0.0	0.0	0.0
6	0.0	8.999940518574936E-5	0.0	0.0	8.181764107795397E-6
7	0.0	9.818116929354476E-5	0.0	1.6363528215590793E-5	8.181764107795397E-6
8	0.0	1.1454469750913555E-4	0.0	1.6363528215590793E-5	8.181764107795397E-6
9	0.0	1.6363528215590793E-4	0.0	4.909058464677238E-5	1.6363528215590793E-5
10-11	8.181764107795397E-6	1.6363528215590793E-4	0.0	5.7272348754567776E-5	1.6363528215590793E-5
12-13	8.181764107795397E-6	1.6772616420980562E-4	0.0	6.136323080846548E-5	1.6363528215590793E-5
14-15	8.181764107795397E-6	2.3727115912606652E-4	0.0	6.545411286236317E-5	2.454529232338619E-5
16-17	1.2272646161693095E-5	2.454529232338619E-4	0.0	8.181764107795397E-5	4.090882053897698E-5
18-19	2.045441026948849E-5	2.7817997966504346E-4	0.0	8.590852313185166E-5	4.090882053897698E-5
20-21	2.454529232338619E-5	3.109070360962251E-4	0.0	1.0227205134744245E-4	4.090882053897698E-5
22-23	2.454529232338619E-5	3.6817938485079284E-4	0.0	1.4318087188641945E-4	5.318146670067008E-5
24-25	4.499970259287468E-5	4.172699694975652E-4	0.0	1.7999881037149873E-4	6.545411286236317E-5
26-27	4.909058464677238E-5	4.33633497713156E-4	0.0	2.9863438993453197E-4	6.545411286236317E-5
28-29	4.909058464677238E-5	4.33633497713156E-4	0.0	9.08175815965289E-4	6.545411286236317E-5
30-31	4.909058464677238E-5	4.663605541443376E-4	0.0	0.0022827121860749156	6.545411286236317E-5
32-33	4.909058464677238E-5	4.786332003060307E-4	0.0	0.005170874916126691	7.363587697015857E-5
34-35	4.909058464677238E-5	5.277237849528031E-4	0.0	0.00917175756483864	8.999940518574936E-5
36-37	4.909058464677238E-5	5.727234875456778E-4	0.0	0.01473535715813951	9.818116929354476E-5
38-39	4.909058464677238E-5	5.849961337073709E-4	0.0	0.022577578055461397	1.0636293340134016E-4
40-41	4.909058464677238E-5	6.38177600408041E-4	0.0	0.03147933740474279	1.1454469750913555E-4
42-43	4.909058464677238E-5	6.627228927314271E-4	0.0	0.03973064650745445	1.1454469750913555E-4
44-45	4.909058464677238E-5	7.281770055937903E-4	0.0	0.04934831021616794	1.1454469750913555E-4
46-47	4.909058464677238E-5	7.854493543483581E-4	0.0	0.0597105144586908	1.3090822572472635E-4
48-49	4.909058464677238E-5	7.895402364022558E-4	0.0	0.06977817519333304	1.5545351804811255E-4
50-51	4.909058464677238E-5	8.631761133724143E-4	0.0	0.07913402245059709	1.5545351804811255E-4
52-53	4.909058464677238E-5	8.67266995426312E-4	0.0	0.08845305176937604	1.7590792831760103E-4
54-55	4.909058464677238E-5	8.795396415880051E-4	0.0	0.09806662459603563	1.9636233858708952E-4
56-57	4.909058464677238E-5	8.836305236419029E-4	0.0	0.10643656927831033	2.004532206409872E-4
58-59	4.909058464677238E-5	9.040849339113913E-4	0.0	0.11379606609327228	2.0454410269488493E-4
60-61	4.909058464677238E-5	9.286302262347775E-4	0.0	0.12106556350304848	2.127258668026803E-4
62-63	4.909058464677238E-5	9.409028723964706E-4	0.0	0.1278277915381414	2.2090763091047572E-4
64-65	4.909058464677238E-5	0.0011168108007140716	0.0	0.13478638191182135	2.2090763091047572E-4
66-67	4.909058464677238E-5	0.0011454469750913556	0.0	0.1416345184700461	2.2090763091047572E-4
68-69	4.909058464677238E-5	0.0012109010879537186	0.0	0.1489081067618762	2.2090763091047572E-4
70-71	5.7272348754567776E-5	0.0012518099084926956	0.0	0.15560488068410674	2.290893950182711E-4
72-73	5.7272348754567776E-5	0.0012722643187621841	0.0	0.16105802646195239	2.454529232338619E-4
74-75	5.7272348754567776E-5	0.001300900493139468	0.0	0.16608981138824655	2.454529232338619E-4
76-77	6.545411286236317E-5	0.0013663546060018314	0.0	0.17038932842689303	2.495438052877596E-4
78-79	6.545411286236317E-5	0.001399081662433013	0.0	0.1736661249520651	2.536346873416573E-4
80-81	6.545411286236317E-5	0.0014072634265408083	0.0	0.1756215665738282	2.699982155572481E-4
82-83	6.545411286236317E-5	0.0014195360727025015	0.0	0.176869285600267	2.945435078806343E-4
84-85	6.545411286236317E-5	0.0014277178368102966	0.0	0.17815791344724474	2.945435078806343E-4
86-87	6.545411286236317E-5	0.0014318087188641944	0.0	0.17854245636031113	2.945435078806343E-4
88-89	6.545411286236317E-5	0.0014563540111875806	0.0	0.17855881988852673	3.068161540423274E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14065	0.0	50.08881	1
GTATCAA	25250	0.0	35.582863	1
TCAACGC	32435	0.0	27.039179	4
ATCAACG	32530	0.0	26.94731	3
CAACGCA	33085	0.0	26.507957	5
TATCAAC	34140	0.0	25.91132	2
AACGCAG	33990	0.0	25.87628	6
ACGCAGA	39795	0.0	22.10164	7
CGCAGAG	40295	0.0	21.816973	8
GTACATG	31935	0.0	19.102367	1
AGAGTAC	42700	0.0	18.774172	10-11
TACATGG	31810	0.0	18.702286	2
GCAGAGT	47175	0.0	18.474997	9
ACATGGG	32085	0.0	18.070103	3
GAGTACT	28495	0.0	17.94517	12-13
AGTACTT	32235	0.0	16.820375	12-13
CAGAGTA	46385	0.0	16.807507	10-11
CATGGGG	23925	0.0	15.810362	4
GTACTTT	32765	0.0	15.639385	14-15
GTGGTAT	6810	0.0	14.672981	1
>>END_MODULE