FastQCFastQC Report
Fri 27 May 2016
SRR2139794_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139794_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9343241
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102250.046.446181
GTATCAA175850.032.8715061
ATCAACG220850.025.3439273
TCAACGC220750.025.2988974
CAACGCA227650.024.5320975
AACGCAG230800.024.305386
TATCAAC237300.023.9386792
ACGCAGA269750.020.7650227
CGCAGAG275300.020.3161968
GTACATG239000.019.3662031
TACATGG241250.018.5133132
AGAGTAC293800.018.08861710-11
ACATGGG240550.017.9612463
GCAGAGT322300.017.09559
GAGTACT189250.016.908412-13
CAGAGTA319000.016.790039
CATGGGG177200.015.9812114
AGTACTT219600.015.78348912-13
GTACTTT218850.014.67013914-15
TATACTG53350.013.4845965
Produced by FastQC (version 0.11.5)