##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139794_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9343241
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.724463919960964	31.0	31.0	34.0	30.0	34.0
2	31.906372959875487	31.0	31.0	34.0	30.0	34.0
3	31.930408516702073	31.0	31.0	34.0	30.0	34.0
4	35.663542233364204	37.0	35.0	37.0	33.0	37.0
5	35.4621393154688	37.0	35.0	37.0	33.0	37.0
6	35.46499785245826	37.0	35.0	37.0	33.0	37.0
7	35.354108922160954	37.0	35.0	37.0	32.0	37.0
8	35.336249701789775	37.0	35.0	37.0	32.0	37.0
9	36.86236103724607	39.0	37.0	39.0	33.0	39.0
10-11	36.7371609059426	39.0	35.0	39.0	32.0	39.0
12-13	36.67795270399212	39.0	35.0	39.0	32.0	39.0
14-15	37.77154041087027	40.0	37.0	41.0	32.0	41.0
16-17	37.74678080122304	39.0	37.0	41.0	32.0	41.0
18-19	37.772067315827556	39.5	37.0	41.0	32.0	41.0
20-21	37.74458665374398	39.5	37.0	41.0	32.0	41.0
22-23	37.67180622244633	39.0	37.0	41.0	32.0	41.0
24-25	37.58309729641374	39.0	36.0	41.0	32.0	41.0
26-27	37.380171882570124	39.0	36.0	41.0	32.0	41.0
28-29	37.267908089368106	39.0	36.0	40.0	31.0	41.0
30-31	37.114809609445714	39.0	36.0	40.0	31.0	41.0
32-33	37.30112813389812	39.0	36.0	40.0	31.0	41.0
34-35	37.25627106477568	39.0	36.0	41.0	31.0	41.0
36-37	37.16514583462565	39.0	36.0	40.5	31.0	41.0
38-39	37.05404651426713	39.0	36.0	40.0	31.0	41.0
40-41	36.957452507794954	39.0	35.0	40.0	30.0	41.0
42-43	36.91179027639249	39.0	35.0	40.0	30.0	41.0
44-45	36.935841735149296	39.0	35.5	40.0	30.5	41.0
46-47	37.050041758402784	39.0	36.0	40.0	31.0	41.0
48-49	36.99277335925245	39.0	35.0	40.0	31.0	41.0
50-51	36.884106269779906	39.0	35.0	40.0	30.0	41.0
52-53	36.775789073234066	39.0	35.0	40.0	30.0	41.0
54-55	36.6419615066408	39.0	35.0	40.0	30.0	41.0
56-57	36.51070542984911	38.0	35.0	40.0	30.0	41.0
58-59	36.36261807472221	38.0	35.0	40.0	30.0	41.0
60-61	36.14135981094806	38.0	34.0	40.0	29.0	41.0
62-63	35.91746247952625	38.0	34.0	40.0	29.0	41.0
64-65	35.70175870288634	37.0	34.0	40.0	29.0	41.0
66-67	35.44301035002914	37.0	34.0	39.5	28.5	41.0
68-69	35.16059750013634	36.0	34.0	39.0	28.0	41.0
70-71	34.86421630495427	36.0	33.0	39.0	28.0	40.0
72-73	34.5616655653089	35.5	33.0	38.5	28.0	40.0
74-75	34.211664051309384	35.0	33.0	38.0	28.0	40.0
76-77	33.18311947444502	34.0	31.5	36.5	26.5	39.0
78-79	33.608395915422456	35.0	32.0	37.0	27.5	39.0
80-81	33.51475706223049	35.0	32.0	36.0	28.0	39.0
82-83	33.288910407225885	35.0	32.0	36.0	27.0	37.5
84-85	33.06701149079659	34.5	32.0	35.5	27.0	37.0
86-87	32.85794472716296	34.0	32.0	35.0	27.0	37.0
88-89	32.65631128180498	34.0	32.0	35.0	27.0	36.0
90-91	32.532153271535364	34.0	32.0	35.0	27.0	36.0
92-93	32.45289668004841	34.0	32.0	35.0	27.0	36.0
94-95	32.41859592291438	34.0	32.0	35.0	27.0	35.0
96-97	32.435943272256864	34.0	31.5	35.0	27.0	35.0
98-99	32.5665960660569	34.0	32.0	35.0	28.0	35.0
100-101	31.943656130862287	33.5	30.5	34.5	27.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	6.0
19	22.0
20	109.0
21	584.0
22	2633.0
23	8778.0
24	22821.0
25	46857.0
26	81521.0
27	125922.0
28	177087.0
29	233099.0
30	292652.0
31	359033.0
32	439143.0
33	542532.0
34	689548.0
35	896440.0
36	1243823.0
37	1733523.0
38	1706069.0
39	719226.0
40	21812.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.982777175500445	22.911332373851856	15.764797247550394	24.341093203097298
2	13.79992293613332	23.259191471598754	40.71684380652688	22.224041785741043
3	16.527937147291823	28.194081689640672	31.88545602109589	23.392525141971614
4	12.058749207047105	19.873339454692434	39.5742119891802	28.49369934908026
5	12.36694255600226	38.619583636895086	36.48797809838751	12.525495708715148
6	28.332160114461352	39.980505693902145	19.163286058874004	12.524048132762497
7	24.654410605484756	33.28546272112643	24.98548415908356	17.07464251430526
8	23.48941871455526	37.55232258270979	21.99549385486257	16.962764847872382
9	25.088285745813472	16.47835049957504	21.94880769959803	36.484556055013456
10-11	23.35768177231006	27.294318962766773	30.067639269927852	19.280359994995315
12-13	24.241299138061407	24.98631363570735	29.916765499252346	20.855621726978892
14-15	21.495153555388328	26.266458287868204	27.315687350888197	24.922700805855268
16-17	21.128257314565683	29.20228109282421	28.01685731963887	21.65260427297123
18-19	21.201379162939133	28.552134179674027	29.52836251506242	20.718124142324417
20-21	21.966315108280128	27.792377060484807	29.28815434068045	20.953153490554612
22-23	21.7116013159354	27.72263253022108	29.0905028875473	21.475263266296217
24-25	21.503884134453966	27.885035209731164	29.141879687320387	21.46920096849448
26-27	21.555022785456735	27.952515442428687	29.030823147658502	21.461638624456075
28-29	21.503303874728445	28.001625497039246	29.078343071538647	21.416727556693658
30-31	21.73228769960777	27.919529453014412	28.937708843013933	21.410474004363884
32-33	21.578516876533964	28.095961519293848	28.85383458368217	21.47168702049002
34-35	21.679026216662105	28.078193307999282	28.878903341247796	21.36387713409082
36-37	21.66219444340219	28.05407544818117	28.829862335307983	21.453867773108655
38-39	21.54285660847655	28.15078354608725	28.888410793609786	21.417949051826408
40-41	21.71857252160477	27.9989172190123	28.77186762578	21.51064263360293
42-43	21.604561516222294	28.173181556971155	28.530367684573122	21.69188924223343
44-45	21.719455007475183	28.208206315071138	28.36782842545244	21.70451025200124
46-47	21.799664458120642	28.168040898941637	28.182288118165417	21.850006524772308
48-49	21.738843711375235	28.293506749173304	28.13118337026197	21.836466169189496
50-51	21.819156837780127	28.316268144538288	27.973224768359177	21.89135024932241
52-53	21.897394806710285	28.266773151247822	27.92253480924554	21.913297232796353
54-55	21.86941367162747	28.138417868138394	28.003380718140075	21.98878774209406
56-57	21.84619217648129	28.24663126840695	27.957936217737373	21.94924033737438
58-59	21.824141315299496	28.290313301679564	27.957450595349005	21.928094787671935
60-61	21.95889478515657	28.286108489830415	27.913873040588435	21.841123684424577
62-63	21.963578102923318	28.31879934260576	27.88833996079682	21.8292825936741
64-65	21.903369346382394	28.235152084577237	27.887104830469273	21.9743737385711
66-67	21.965753538708775	28.26567988597978	27.76206439361923	22.006502181692223
68-69	21.85502608096848	28.22639215824368	27.875665461893522	22.04291629889432
70-71	21.91163897167095	28.040039738856343	27.907873779395775	22.14044751007693
72-73	21.934795276531258	28.130748417188823	27.834228522751964	22.100227783527956
74-75	21.839684813294326	28.20047886538342	27.827525650258906	22.132310671063347
76-77	21.936265822866226	28.120114145146648	27.812747688489015	22.130872343498112
78-79	21.8393170635215	28.143937832944584	27.794316829996674	22.222428273537243
80-81	21.94482343069523	28.117604376580267	27.779373244207022	22.15819894851748
82-83	21.9278686070778	28.048552140339854	27.897976606716636	22.12560264586571
84-85	21.927848723859753	28.10138069870236	27.851603960512954	22.119166616924925
86-87	21.85522367019404	28.099125766460297	27.906152044701688	22.139498518643975
88-89	21.890866615448118	27.99951356297376	27.887746379906424	22.221873441671697
90-91	21.91016337282519	28.024271847979982	27.96542805704474	22.10013672215009
92-93	21.97811891294194	27.93391490843667	27.92145203014852	22.16651414847287
94-95	21.740234021512777	27.922851529263504	28.07268935802327	22.264225091200448
96-97	21.700048464648614	27.99850338675093	28.134805603683017	22.16664254491744
98-99	21.83754154353083	28.612763696586857	28.18518791148114	21.36450684840117
100-101	22.349041913524662	31.36367591091689	25.401160451555743	20.886121724002706
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1947.0
1	1810.5
2	2676.5
3	3860.5
4	5059.5
5	7567.5
6	9615.0
7	11260.5
8	12476.5
9	12636.0
10	12000.5
11	11329.5
12	12587.0
13	16468.0
14	25643.0
15	38369.5
16	47894.0
17	52160.0
18	53203.5
19	52657.5
20	51387.0
21	51513.0
22	54428.0
23	61985.5
24	74775.5
25	92637.0
26	113631.0
27	134122.5
28	155294.0
29	177085.0
30	196637.5
31	216843.5
32	237952.5
33	258867.0
34	279794.0
35	299167.0
36	319383.0
37	337471.0
38	350900.5
39	359667.0
40	366602.5
41	373860.0
42	378241.5
43	380229.0
44	382132.5
45	383640.5
46	382093.5
47	378059.0
48	373514.5
49	367585.5
50	357686.5
51	344893.0
52	328912.5
53	310067.5
54	290727.5
55	269455.5
56	246968.5
57	223410.0
58	198691.0
59	171612.5
60	143382.0
61	117500.0
62	93663.5
63	71987.0
64	53519.5
65	40240.0
66	30831.0
67	23262.0
68	17330.5
69	12610.5
70	8874.5
71	6068.5
72	4166.5
73	2858.5
74	1941.5
75	1278.0
76	869.0
77	635.5
78	462.5
79	332.5
80	238.5
81	177.5
82	141.0
83	96.0
84	60.5
85	47.0
86	37.0
87	29.5
88	22.0
89	16.0
90	11.0
91	8.0
92	6.5
93	3.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0036496971447059967
3	0.0
4	0.0
5	2.14058483560469E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	4.0135965667587937E-4
20-21	5.512274390101625E-4
22-23	2.462127395823911E-4
24-25	1.445575991359953E-4
26-27	0.002753291871114658
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.03242819020463469
38-39	0.01621238453392101
40-41	0.021376550667880814
42-43	0.009952802819860771
44-45	0.0040348650500382196
46-47	0.0051366817081572435
48-49	0.009205244762809709
50-51	0.006940523367646307
52-53	0.011105873338480557
54-55	0.003417497385584937
56-57	0.016662822702486316
58-59	0.0133105599042897
60-61	0.013055433001627495
62-63	0.0049910683378684845
64-65	0.006655241418569713
66-67	3.735489364935151E-4
68-69	5.003025868530102E-4
70-71	0.0010662101553526708
72-73	9.884426019288601E-5
74-75	0.002711342104992516
76-77	0.003479831297237102
78-79	0.002843427294182224
80-81	4.882870742947618E-4
82-83	0.002934031906959155
84-85	0.004420759222916928
86-87	0.0014199268137515667
88-89	0.07221994022874192
90-91	8.890318094061693E-4
92-93	0.0
94-95	4.6916045327261284E-4
96-97	0.011072663453028115
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	269.0
20-21	1036.0
22-23	2019.0
24-25	4294.0
26-27	5975.0
28-29	9631.0
30-31	22846.0
32-33	29588.0
34-35	42106.0
36-37	63499.0
38-39	71109.0
40-41	73480.0
42-43	83963.0
44-45	98500.0
46-47	99397.0
48-49	90993.0
50-51	83710.0
52-53	82269.0
54-55	89277.0
56-57	94188.0
58-59	94682.0
60-61	93639.0
62-63	89938.0
64-65	94599.0
66-67	101011.0
68-69	104154.0
70-71	104483.0
72-73	100501.0
74-75	101376.0
76-77	103032.0
78-79	111277.0
80-81	113635.0
82-83	110686.0
84-85	111741.0
86-87	118484.0
88-89	127355.0
90-91	140075.0
92-93	145972.0
94-95	172930.0
96-97	235733.0
98-99	770072.0
100-101	5149717.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.51223216780251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17812595233865	77.1133284289372
2	6.784975930840294	11.603968741019342
3	1.6649165150162786	4.271121827162419
4	0.6448402756793982	2.2056692546018586
5	0.2903690960059561	1.2415054776008125
6	0.15226610191088755	0.7812368554734055
7	0.07971894871537638	0.4771861675505685
8	0.04937179183316873	0.3377513700622672
9	0.034856995536247666	0.2682629545470899
>10	0.1181149562482548	1.5346819861805658
>50	0.0021820242549459795	0.11877460042857153
>100	2.614116204818449E-4	0.04651233643589832
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.28116967120938E-5	0.0	0.0	0.0
3	0.0	4.28116967120938E-5	0.0	0.0	0.0
4	0.0	2.0335555938244556E-4	0.0	0.0	0.0
5	0.0	2.0335555938244556E-4	0.0	0.0	0.0
6	0.0	2.0335555938244556E-4	0.0	0.0	0.0
7	0.0	2.0335555938244556E-4	0.0	0.0	0.0
8	0.0	2.2476140773849246E-4	0.0	0.0	0.0
9	0.0	3.3179064951872694E-4	0.0	0.0	0.0
10-11	0.0	3.3179064951872694E-4	0.0	0.0	0.0
12-13	0.0	3.3179064951872694E-4	0.0	0.0	0.0
14-15	0.0	4.4417135338797317E-4	0.0	3.7460234623082075E-5	0.0
16-17	0.0	4.709286638330318E-4	0.0	5.351462089011725E-5	0.0
18-19	0.0	5.030374363671022E-4	0.0	5.351462089011725E-5	0.0
20-21	0.0	5.45849133079196E-4	0.0	6.956900715715243E-5	0.0
22-23	0.0	6.154181402363484E-4	0.0	8.027193133517587E-5	1.070292417802345E-5
24-25	0.0	7.01041533660536E-4	0.0	1.0167777969122277E-4	3.210877253407035E-5
26-27	0.0	7.385017682836181E-4	0.0	1.8730117311541037E-4	3.210877253407035E-5
28-29	0.0	7.385017682836181E-4	0.0	5.351462089011725E-4	3.210877253407035E-5
30-31	0.0	8.027193133517587E-4	0.0	0.0014877064607452598	3.7460234623082075E-5
32-33	0.0	8.027193133517587E-4	0.0	0.004104571422271993	5.351462089011725E-5
34-35	0.0	8.990456309539699E-4	0.0	0.007743565642799967	5.351462089011725E-5
36-37	0.0	9.846690243781573E-4	0.0	0.012886320710340234	5.351462089011725E-5
38-39	0.0	0.0010060748727342044	0.0	0.020233878158553334	5.351462089011725E-5
40-41	0.0	0.0010809953419803685	0.0	0.02831993737505005	5.351462089011725E-5
42-43	0.0	0.0011398614249594975	0.0	0.036095611790384084	5.351462089011725E-5
44-45	0.0	0.0012789994392738023	0.0	0.04572289208851618	5.351462089011725E-5
46-47	0.0	0.001380677218965025	0.0	0.05606191684448683	5.351462089011725E-5
48-49	0.0	0.0013860286810540368	0.0	0.0658122807706662	6.42175450681407E-5
50-51	0.0	0.001498409384923283	0.0	0.07569643124907086	6.956900715715243E-5
52-53	0.0	0.0015091123091013066	0.0	0.08505613844275237	8.027193133517587E-5
54-55	0.0	0.0015251666953683416	0.0	0.09473158189968556	8.56233934241876E-5
56-57	0.0	0.0015519240058134002	0.0	0.10496892887596498	8.56233934241876E-5
58-59	0.0	0.0015519240058134002	0.0	0.11389516764043656	8.56233934241876E-5
60-61	0.0	0.0015626269299914238	0.0	0.12243074967241024	8.56233934241876E-5
62-63	0.0	0.001637547399237588	0.0	0.13047399719219488	8.56233934241876E-5
64-65	0.0	0.0019158234278661976	0.0	0.1389774704516345	8.56233934241876E-5
66-67	0.0	0.0019318778141332329	0.0	0.14690298580546085	9.097485551319933E-5
68-69	0.0	0.002006798283379397	0.0	0.1549515847873345	9.632631760221105E-5
70-71	0.0	0.002097773138892596	0.0	0.16289315452742792	1.070292417802345E-4
72-73	0.0	0.0021405848356046897	0.0	0.16897241546054523	1.1773216595825795E-4
74-75	0.0	0.002199450918583819	0.0	0.17413657637644153	1.2308362804726967E-4
76-77	0.0	0.002317183084542077	0.0	0.17884586301477184	1.284350901362814E-4
78-79	0.0	0.002381400629610218	0.0	0.1829718402853999	1.284350901362814E-4
80-81	0.0	0.0024081579400552764	0.0	0.1854816760051464	1.5519240058134003E-4
82-83	0.0	0.002440266712589347	0.0	0.18669110643726305	1.8194971102639866E-4
84-85	0.0	0.002456321098856382	0.0	0.18798080880071488	1.8194971102639866E-4
86-87	0.0	0.002515187181835511	0.0	0.18841427722992482	1.8194971102639866E-4
88-89	0.0	0.0025794047269036514	0.0	0.1884570889266369	1.8194971102639866E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10225	0.0	46.44618	1
GTATCAA	17585	0.0	32.871506	1
ATCAACG	22085	0.0	25.343927	3
TCAACGC	22075	0.0	25.298897	4
CAACGCA	22765	0.0	24.532097	5
AACGCAG	23080	0.0	24.30538	6
TATCAAC	23730	0.0	23.938679	2
ACGCAGA	26975	0.0	20.765022	7
CGCAGAG	27530	0.0	20.316196	8
GTACATG	23900	0.0	19.366203	1
TACATGG	24125	0.0	18.513313	2
AGAGTAC	29380	0.0	18.088617	10-11
ACATGGG	24055	0.0	17.961246	3
GCAGAGT	32230	0.0	17.0955	9
GAGTACT	18925	0.0	16.9084	12-13
CAGAGTA	31900	0.0	16.79003	9
CATGGGG	17720	0.0	15.981211	4
AGTACTT	21960	0.0	15.783489	12-13
GTACTTT	21885	0.0	14.670139	14-15
TATACTG	5335	0.0	13.484596	5
>>END_MODULE