FastQCFastQC Report
Fri 27 May 2016
SRR2139793_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139793_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13901776
Sequences flagged as poor quality0
Sequence length19-101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA148050.048.8862531
GTATCAA263500.034.5930561
TCAACGC338600.026.2872094
ATCAACG341600.026.1055343
CAACGCA349850.025.3938735
TATCAAC362300.024.8927882
AACGCAG359950.024.7280146
ACGCAGA417850.021.3216467
CGCAGAG425500.020.9185648
GTACATG355900.018.4594421
AGAGTAC452400.018.4444810-11
TACATGG353350.018.2835732
ACATGGG354350.017.496633
GCAGAGT503150.017.4064049
GAGTACT295000.017.2077912-13
CAGAGTA489450.016.9324289
AGTACTT325400.016.65869312-13
CATGGGG267400.015.3473584
GTACTTT337950.015.01542514-15
TATACCG11550.014.1834895
Produced by FastQC (version 0.11.5)