##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139793_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13901776
Sequences flagged as poor quality	0
Sequence length	19-101
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.53512637521997	31.0	31.0	34.0	30.0	34.0
2	31.72957383286855	31.0	31.0	34.0	30.0	34.0
3	31.74492410178383	31.0	31.0	34.0	30.0	34.0
4	35.51432342169806	37.0	35.0	37.0	33.0	37.0
5	35.29722907346515	37.0	35.0	37.0	33.0	37.0
6	35.30159642911812	37.0	35.0	37.0	32.0	37.0
7	35.185731664788726	37.0	35.0	37.0	32.0	37.0
8	35.166301989040825	37.0	35.0	37.0	32.0	37.0
9	36.664108096692104	39.0	35.0	39.0	32.0	39.0
10-11	36.519312352608765	38.0	35.0	39.0	32.0	39.0
12-13	36.458129234710725	38.0	35.0	39.0	32.0	39.0
14-15	37.49545759477063	39.0	36.0	41.0	32.0	41.0
16-17	37.46303443531244	39.0	36.0	41.0	32.0	41.0
18-19	37.4843684720571	39.0	36.0	41.0	32.0	41.0
20-21	37.45818227318864	39.0	36.0	40.0	32.0	41.0
22-23	37.38046630370148	39.0	36.0	40.0	32.0	41.0
24-25	37.28468550803052	39.0	36.0	40.0	31.0	41.0
26-27	37.06393330613848	39.0	36.0	40.0	31.0	41.0
28-29	36.937629938072064	39.0	36.0	40.0	31.0	41.0
30-31	36.77412957195689	38.5	35.5	40.0	30.0	41.0
32-33	36.964030798073914	39.0	36.0	40.0	31.0	41.0
34-35	36.91123410440881	39.0	35.5	40.0	30.5	41.0
36-37	36.809330013702066	38.5	35.0	40.0	30.0	41.0
38-39	36.685551917950114	38.0	35.0	40.0	30.0	41.0
40-41	36.582314562675975	38.0	35.0	40.0	30.0	41.0
42-43	36.51977275834127	38.0	35.0	40.0	30.0	41.0
44-45	36.5477499208844	38.0	35.0	40.0	30.0	41.0
46-47	36.66558722182412	38.0	35.0	40.0	30.0	41.0
48-49	36.60338290021082	38.5	35.0	40.0	30.0	41.0
50-51	36.48875115514518	38.0	35.0	40.0	30.0	41.0
52-53	36.378378308693904	38.0	35.0	40.0	30.0	41.0
54-55	36.23874276228795	38.0	34.5	40.0	29.0	41.0
56-57	36.10334099496926	38.0	34.0	40.0	29.0	41.0
58-59	35.94711949649361	38.0	34.0	40.0	29.0	41.0
60-61	35.7207283287694	38.0	34.0	40.0	28.0	41.0
62-63	35.49224987494387	37.0	34.0	40.0	28.0	41.0
64-65	35.28109942793904	37.0	33.5	40.0	28.0	41.0
66-67	35.01705153598536	36.5	33.0	39.0	28.0	40.5
68-69	34.72971400465507	36.0	33.0	39.0	28.0	40.0
70-71	34.43273648704243	36.0	32.5	39.0	27.5	40.0
72-73	34.126751523968906	35.0	32.0	38.0	27.0	40.0
74-75	33.77313991073852	35.0	32.0	37.5	26.0	39.5
76-77	32.79043106675088	34.0	31.0	36.0	26.0	39.0
78-79	33.231053771065774	34.5	31.5	36.0	26.0	39.0
80-81	33.143570274629255	34.5	32.0	36.0	26.0	38.5
82-83	32.92328063592946	34.0	31.5	36.0	26.0	37.5
84-85	32.705007748936254	34.0	31.0	35.0	26.0	37.0
86-87	32.4914831545134	34.0	31.0	35.0	26.0	36.5
88-89	32.29041299076444	34.0	31.0	35.0	26.0	36.0
90-91	32.170537284678204	34.0	31.0	35.0	26.0	36.0
92-93	32.09841615423212	34.0	31.0	35.0	26.0	35.5
94-95	32.07008434021161	34.0	31.0	35.0	26.0	35.0
96-97	32.100776918199095	34.0	31.0	35.0	26.0	35.0
98-99	32.262787982677416	34.0	31.0	35.0	27.0	35.0
100-101	31.644424408329662	33.0	30.0	34.5	25.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	1.0
13	0.0
14	1.0
15	0.0
16	0.0
17	2.0
18	6.0
19	24.0
20	211.0
21	1219.0
22	5457.0
23	17118.0
24	42767.0
25	85518.0
26	147492.0
27	222817.0
28	310441.0
29	399831.0
30	497127.0
31	601336.0
32	725754.0
33	891476.0
34	1119668.0
35	1440817.0
36	1946345.0
37	2524691.0
38	2165221.0
39	743676.0
40	12758.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.02517404970415	23.452586201935638	16.334459712197923	24.187780036162284
2	14.019103635177885	23.990158428214407	39.97275677523433	22.017981161373378
3	16.72718651199674	28.361002220147984	32.12985880365214	22.781952464203133
4	12.41678761044632	20.524384798028684	39.036616616466844	28.022210975058147
5	12.357279895161573	38.73168551953769	36.426792467406855	12.484242117893883
6	28.309620295996712	40.308921680222724	18.936371870759537	12.445086153021025
7	24.503595799558273	33.39639482034526	24.90410577756396	17.19590360253251
8	23.497580453029887	37.82608063890542	21.829527392759026	16.84681151530567
9	25.138126236532653	16.353586764741426	21.65867152513463	36.84961547359129
10-11	23.42792028874584	27.385709566892746	29.997178058400596	19.189192085960816
12-13	24.32732695448409	25.01928170904207	29.841090088057815	20.81230124841603
14-15	21.541182939503557	26.288651895987964	27.28767173345334	24.88249343105514
16-17	21.196475184177906	29.20332625126459	27.96776469423763	21.63243387031988
18-19	21.261517947210663	28.51822706795058	29.516833104602785	20.70342188023597
20-21	22.019684852586614	27.732829221780484	29.28574041456262	20.96174551107028
22-23	21.744876758659537	27.665557199734337	29.16508590232571	21.424480139280416
24-25	21.554366664925002	27.83187976232167	29.145059578634974	21.46869399411835
26-27	21.593452127260097	27.91849174671745	29.045377194731444	21.442678931291013
28-29	21.58139655528324	27.979095658276375	29.069863789981003	21.369643996459384
30-31	21.83876579008885	27.83858926910393	28.953257611096856	21.36938732971036
32-33	21.674330137486287	27.98363413605511	28.886268227394936	21.455767499063672
34-35	21.773651722910287	27.97716925437353	28.918376477088408	21.330802545627776
36-37	21.722751323830472	27.947660042163424	28.901139216620443	21.42844941738566
38-39	21.63116457584013	28.034607178253857	28.921119144043868	21.413109101862148
40-41	21.81694383830192	27.970727051189442	28.75688686674553	21.45544224376311
42-43	21.66470292097448	28.095024977745286	28.589214079097903	21.651058022182323
44-45	21.815692422112555	28.11271001037933	28.40219260264795	21.669404964860163
46-47	21.879616603662583	28.116007841085892	28.208536350450657	21.795839204800867
48-49	21.78482222652368	28.255124355321374	28.173858141424706	21.78619527673024
50-51	21.84413275194584	28.311215549019693	28.034861942086525	21.809789756947943
52-53	21.970587306524923	28.19940711726393	27.971144966576805	21.85886060963434
54-55	21.896850811887784	28.09261365957594	28.076653237337723	21.933882291198554
56-57	21.920945707187872	28.178637935077646	28.012601060942778	21.887815296791707
58-59	21.92985564191898	28.281452485251318	27.94345166855663	21.845240204273072
60-61	22.044652738772548	28.214562061391195	27.965773859796357	21.775011340039896
62-63	22.00628977440718	28.252862864964452	27.94609831406391	21.794749046564462
64-65	21.924760101322928	28.24318975721981	27.931135449744072	21.900914691713186
66-67	21.997661758031693	28.278890778166087	27.80419837828907	21.919249085513147
68-69	21.94906136217916	28.243024781024168	27.871012820040015	21.93690103675666
70-71	21.9533344577133	28.05549291132463	27.941425378456564	22.049747252505508
72-73	22.013102563571294	28.11817022803541	27.854430130397105	22.01429707799619
74-75	21.85334458150526	28.191629920477585	27.868941608559567	22.086083889457583
76-77	21.949155628716188	28.106237131653078	27.87785037130066	22.066756868330074
78-79	21.924184975128437	28.15639435548353	27.835197790949994	22.08422287843803
80-81	21.963430882145087	28.136747767294874	27.814969587207077	22.084851763352958
82-83	21.927347279311494	28.097807001520252	27.943013577906132	22.031832141262118
84-85	21.979136859328992	28.082930241103753	27.89311774584256	22.04481515372469
86-87	21.89614918367019	28.095106023161488	27.936886836315654	22.07185795685267
88-89	21.912219910341125	28.05673407487408	27.927464280179542	22.103581734605253
90-91	21.921579211704685	28.072557338940936	28.008683698571957	21.997179750782426
92-93	21.965839606257905	27.961007550897154	27.994123057221504	22.07902978562344
94-95	21.73113409147919	27.988918657570512	28.120042570767982	22.159904680182315
96-97	21.645628088551337	28.081731199750454	28.184660168223957	22.08798054347425
98-99	21.774949158792516	28.705115748178113	28.270597991179002	21.249337101850365
100-101	22.32799735427063	31.479536758068573	25.495206176108248	20.697259711552555
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3897.0
1	3339.0
2	4144.5
3	5796.5
4	7348.0
5	10555.0
6	13312.5
7	16223.5
8	18561.0
9	18906.0
10	17870.0
11	16638.5
12	18421.5
13	24238.0
14	37874.5
15	57217.0
16	72436.0
17	79232.0
18	80044.0
19	78010.0
20	74724.0
21	72467.5
22	74295.5
23	83226.5
24	100173.0
25	124833.0
26	155822.0
27	188109.0
28	220930.0
29	254417.0
30	284647.5
31	314680.5
32	346241.5
33	377124.0
34	406331.5
35	434195.0
36	463988.5
37	489365.0
38	508096.5
39	522659.5
40	537285.0
41	551961.5
42	559000.0
43	565231.5
44	571950.5
45	576054.5
46	576181.0
47	572049.5
48	565072.0
49	551055.0
50	533833.0
51	514639.0
52	488258.5
53	459169.5
54	429084.0
55	394624.5
56	360458.0
57	325320.5
58	286987.0
59	246751.0
60	206217.5
61	168135.0
62	133333.5
63	102926.0
64	76732.5
65	56644.5
66	42192.0
67	31986.0
68	24046.5
69	17173.0
70	12087.5
71	8427.5
72	5757.5
73	3890.5
74	2631.5
75	1764.0
76	1233.5
77	887.0
78	627.0
79	464.0
80	359.0
81	267.0
82	205.0
83	144.0
84	89.5
85	63.0
86	49.5
87	36.5
88	25.5
89	15.5
90	10.5
91	8.5
92	6.5
93	4.0
94	1.5
95	1.0
96	0.5
97	0.0
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0024313440239578025
3	0.0
4	0.0
5	2.157997654400416E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	3.4168296194673256E-4
20-21	5.323371243529812E-4
22-23	1.5828603279010288E-4
24-25	1.1515130665786143E-4
26-27	0.002336537509905533
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.030464375832017533
38-39	0.014130515700442738
40-41	0.019927993663761082
42-43	0.008664800341003738
44-45	0.003234651839392452
46-47	0.004657448981906264
48-49	0.008440295369800049
50-51	0.006480628498222227
52-53	0.010157710426373886
54-55	0.0030128661167537862
56-57	0.015438109306018511
58-59	0.01223128837306231
60-61	0.01215883570683095
62-63	0.004301935805621594
64-65	0.00581934759777993
66-67	2.8443649658228636E-4
68-69	2.3287477044898764E-4
70-71	6.56098421967513E-4
72-73	8.687370088524736E-5
74-75	0.001737880996860859
76-77	0.0020995004526184204
78-79	0.0017760082168172524
80-81	2.981839861253615E-4
82-83	0.002684045363348913
84-85	0.004027525939089137
86-87	0.0012842848128126025
88-89	0.06669719010545079
90-91	8.640272349375455E-4
92-93	1.5319430031414023E-5
94-95	4.816859090588522E-4
96-97	0.010488510622845695
98-99	0.0
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	469.0
20-21	1701.0
22-23	3351.0
24-25	6270.0
26-27	8307.0
28-29	12758.0
30-31	28585.0
32-33	37033.0
34-35	52781.0
36-37	80189.0
38-39	90888.0
40-41	96442.0
42-43	111545.0
44-45	132345.0
46-47	133939.0
48-49	122427.0
50-51	113077.0
52-53	111296.0
54-55	121101.0
56-57	128763.0
58-59	130332.0
60-61	131408.0
62-63	125350.0
64-65	132760.0
66-67	143072.0
68-69	148082.0
70-71	150258.0
72-73	146114.0
74-75	147939.0
76-77	148851.0
78-79	162717.0
80-81	170064.0
82-83	165670.0
84-85	167108.0
86-87	179829.0
88-89	194996.0
90-91	216798.0
92-93	231840.0
94-95	280478.0
96-97	381648.0
98-99	1192588.0
100-101	7760607.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.85130965848101
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.51291906626864	77.70652642851259
2	6.673576123735796	11.458705006565744
3	1.5618762378329225	4.022673616272527
4	0.5541165806895539	1.9028653662271033
5	0.2667579670276197	1.1450760415577526
6	0.1408590017054207	0.7255757864157947
7	0.08182748751974254	0.49174978787430357
8	0.04877109023432389	0.3349649576070928
9	0.03361805554332775	0.259753868690962
>10	0.1221055431334732	1.6823450195324607
>50	0.0028799028802173094	0.16191544656992057
>100	6.513728117019524E-4	0.08459461654103914
>500	4.157061730177281E-5	0.023254057632774754
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.157997654400416E-5	0.0	0.0	0.0
3	0.0	2.157997654400416E-5	0.0	7.193325514668054E-6	0.0
4	0.0	8.631990617601664E-5	0.0	7.193325514668054E-6	0.0
5	0.0	9.35132316906847E-5	0.0	7.193325514668054E-6	0.0
6	7.193325514668054E-6	9.35132316906847E-5	0.0	7.193325514668054E-6	0.0
7	7.193325514668054E-6	9.35132316906847E-5	0.0	2.157997654400416E-5	0.0
8	7.193325514668054E-6	9.35132316906847E-5	0.0	2.157997654400416E-5	0.0
9	7.193325514668054E-6	2.2299309095470966E-4	0.0	6.473992963201249E-5	0.0
10-11	1.4386651029336109E-5	2.2299309095470966E-4	0.0	6.833659238934651E-5	0.0
12-13	2.157997654400416E-5	2.2299309095470966E-4	0.0	7.193325514668053E-5	0.0
14-15	2.517663930133819E-5	2.7334636955738604E-4	0.0	7.552991790401456E-5	1.0789988272002082E-5
16-17	2.8773302058672217E-5	2.7334636955738604E-4	0.0	9.710989444801872E-5	1.4386651029336109E-5
18-19	2.8773302058672217E-5	2.985230088587243E-4	0.0	1.2228653374935693E-4	2.157997654400416E-5
20-21	2.8773302058672217E-5	3.2729631091739646E-4	0.0	1.438665102933611E-4	2.157997654400416E-5
22-23	3.596662757334027E-5	3.740529267627388E-4	0.0	1.9421978889603745E-4	2.157997654400416E-5
24-25	5.3949941360010404E-5	4.1361621709341313E-4	0.0	2.66153044042718E-4	2.8773302058672217E-5
26-27	5.7546604117344434E-5	4.387928563947513E-4	0.0	5.574827273867742E-4	2.8773302058672217E-5
28-29	5.7546604117344434E-5	4.423895191520853E-4	0.0	0.00236660409432579	2.8773302058672217E-5
30-31	7.193325514668053E-5	4.675661584534235E-4	0.0	0.006747339332758635	2.8773302058672217E-5
32-33	7.193325514668053E-5	4.783561467254256E-4	0.0	0.017375477780680684	2.8773302058672217E-5
34-35	7.552991790401456E-5	5.574827273867742E-4	0.0	0.030747869912448593	4.315995308800832E-5
36-37	7.91265806613486E-5	6.438026335627908E-4	0.0	0.047368048514089135	4.315995308800832E-5
38-39	7.91265806613486E-5	6.653826101067949E-4	0.0	0.06941918787930405	5.035327860267638E-5
40-41	7.91265806613486E-5	7.624925045548138E-4	0.0	0.09052080827658279	5.035327860267638E-5
42-43	7.91265806613486E-5	7.768791555841498E-4	0.0	0.10760495637391942	5.035327860267638E-5
44-45	8.631990617601664E-5	8.308290969441602E-4	0.0	0.12540484035996552	5.3949941360010404E-5
46-47	9.710989444801872E-5	8.703923872748345E-4	0.0	0.1421580954836274	5.7546604117344434E-5
48-49	1.0430321996268678E-4	8.703923872748345E-4	0.0	0.1549370382604352	5.7546604117344434E-5
50-51	1.0789988272002081E-4	9.603089562081851E-4	0.0	0.1656658832655626	5.7546604117344434E-5
52-53	1.0789988272002081E-4	9.639056189655192E-4	0.0	0.17481938998297772	5.7546604117344434E-5
54-55	1.0789988272002081E-4	9.639056189655192E-4	0.0	0.1839189467590328	6.114326687467846E-5
56-57	1.1149654547735485E-4	9.926789210241915E-4	0.0	0.19207258122990906	6.473992963201249E-5
58-59	1.1509320823468887E-4	0.0010034689092961934	0.0	0.19965074965961183	6.833659238934651E-5
60-61	1.1509320823468887E-4	0.0010250488858401976	0.0	0.20727567470515998	7.193325514668053E-5
62-63	1.1509320823468887E-4	0.0010646121761708719	0.0	0.21490779307622276	7.91265806613486E-5
64-65	1.1509320823468887E-4	0.0012084786864642331	0.0	0.2223600783094189	7.91265806613486E-5
66-67	1.1509320823468887E-4	0.001212075349221567	0.0	0.23026194638728176	7.91265806613486E-5
68-69	1.1509320823468887E-4	0.0012624286278242434	0.0	0.2375847517612138	7.91265806613486E-5
70-71	1.1509320823468887E-4	0.0012840086043682478	0.0	0.24458745414974314	9.35132316906847E-5
72-73	1.1509320823468887E-4	0.0012983952553975837	0.0	0.2500220115760749	1.0789988272002081E-4
74-75	1.1509320823468887E-4	0.001341555208485592	0.0	0.2548019763805718	1.0789988272002081E-4
76-77	1.1509320823468887E-4	0.0013883118243309344	0.0	0.2589129619122046	1.0789988272002081E-4
78-79	1.1509320823468887E-4	0.0014098918008749386	0.0	0.262675071156376	1.0789988272002081E-4
80-81	1.1509320823468887E-4	0.0014098918008749386	0.0	0.2649265820424671	1.1149654547735485E-4
82-83	1.2228653374935693E-4	0.0014134884636322726	0.0	0.26623217062337934	1.1509320823468887E-4
84-85	1.2947985926402498E-4	0.0014170851263896067	0.0	0.26754495252980626	1.1509320823468887E-4
86-87	1.2947985926402498E-4	0.0014386651029336108	0.0	0.26795856874689966	1.1868987099202289E-4
88-89	1.2947985926402498E-4	0.001489018381536287	0.0	0.2680448886530757	1.3667318477869302E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14805	0.0	48.886253	1
GTATCAA	26350	0.0	34.593056	1
TCAACGC	33860	0.0	26.287209	4
ATCAACG	34160	0.0	26.105534	3
CAACGCA	34985	0.0	25.393873	5
TATCAAC	36230	0.0	24.892788	2
AACGCAG	35995	0.0	24.728014	6
ACGCAGA	41785	0.0	21.321646	7
CGCAGAG	42550	0.0	20.918564	8
GTACATG	35590	0.0	18.459442	1
AGAGTAC	45240	0.0	18.44448	10-11
TACATGG	35335	0.0	18.283573	2
ACATGGG	35435	0.0	17.49663	3
GCAGAGT	50315	0.0	17.406404	9
GAGTACT	29500	0.0	17.20779	12-13
CAGAGTA	48945	0.0	16.932428	9
AGTACTT	32540	0.0	16.658693	12-13
CATGGGG	26740	0.0	15.347358	4
GTACTTT	33795	0.0	15.015425	14-15
TATACCG	1155	0.0	14.183489	5
>>END_MODULE