FastQCFastQC Report
Fri 27 May 2016
SRR2139792_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139792_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5977861
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69650.046.8585661
GTATCAA123500.036.8564571
TCAACGC139300.032.487524
ATCAACG140650.032.082883
CAACGCA144150.031.3944595
AACGCAG148300.030.5725636
TATCAAC149600.030.2808442
ACGCAGA169600.026.681437
CGCAGAG173150.026.1846568
GTGGTAT37950.023.0479931
TGGTATC36550.022.2639962
GCAGAGT210200.021.5693289
GTACATG153400.021.0488031
TACATGG155000.020.7192272
ACATGGG153400.020.2254643
GAGTACT120750.020.08904812-13
CAGAGTA202500.019.12346610-11
CATGGGG112400.018.6214334
AGAGTAC185200.017.94956810-11
GTACTTT139450.017.39514414-15
Produced by FastQC (version 0.11.5)