FastQCFastQC Report
Fri 27 May 2016
SRR2139791_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139791_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11452202
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128050.045.401891
GTATCAA229300.035.8183981
TCAACGC265250.030.5416764
ATCAACG268650.030.139143
CAACGCA273850.029.566845
AACGCAG284000.028.5091426
TATCAAC287850.028.3833832
ACGCAGA335650.024.1605727
CGCAGAG339100.023.927448
GTGGTAT70150.022.98691
TGGTATC72850.020.7183132
GCAGAGT408650.019.8656449
GAGTACT246500.018.56867612-13
GTACATG289850.018.5444321
TACATGG291450.018.4868892
AGAGTAC365050.018.10396610-11
ACATGGG295550.017.5898723
CAGAGTA396300.017.45758410-11
GTACTTT274950.016.6005414-15
CATGGGG217600.016.1051984
Produced by FastQC (version 0.11.5)