##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139791_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11452202
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.511477443377267	31.0	18.0	33.0	18.0	33.0
2	30.456514389110495	32.0	29.0	33.0	25.0	33.0
3	31.972782876166523	33.0	32.0	33.0	29.0	34.0
4	31.61457656789498	33.0	31.0	33.0	29.0	34.0
5	32.58786973893754	33.0	33.0	33.0	32.0	34.0
6	36.1131977937518	38.0	36.0	38.0	33.0	38.0
7	36.829595391349194	38.0	37.0	38.0	35.0	38.0
8	37.03617906844465	38.0	38.0	38.0	36.0	38.0
9	37.12477338419284	38.0	38.0	38.0	36.0	38.0
10-11	37.18525812765091	38.0	38.0	38.0	36.0	38.0
12-13	37.20680140814841	38.0	38.0	38.0	36.0	38.0
14-15	37.2165975591419	38.0	38.0	38.0	36.0	38.0
16-17	37.183247859232665	38.0	38.0	38.0	36.0	38.0
18-19	37.20714282720476	38.0	38.0	38.0	36.0	38.0
20-21	37.22829433019553	38.0	38.0	38.0	36.0	38.0
22-23	37.233406285151574	38.0	38.0	38.0	36.0	38.0
24-25	37.238068654420815	38.0	38.0	38.0	36.0	38.0
26-27	37.208774749560334	38.0	38.0	38.0	36.0	38.0
28-29	37.18262793579839	38.0	38.0	38.0	36.0	38.0
30-31	37.184927045863105	38.0	38.0	38.0	36.0	38.0
32-33	37.176714627907444	38.0	38.0	38.0	36.0	38.0
34-35	37.157347720962036	38.0	38.0	38.0	36.0	38.0
36-37	37.140171961028486	38.0	38.0	38.0	36.0	38.0
38-39	37.125179438020794	38.0	38.0	38.0	36.0	38.0
40-41	37.10456924979009	38.0	38.0	38.0	36.0	38.0
42-43	37.08423607353549	38.0	38.0	38.0	36.0	38.0
44-45	37.04306271561036	38.0	38.0	38.0	36.0	38.0
46-47	37.02464492328839	38.0	38.0	38.0	36.0	38.0
48-49	36.9970499865441	38.0	38.0	38.0	36.0	38.0
50-51	36.96049274555027	38.0	38.0	38.0	36.0	38.0
52-53	36.93737229159692	38.0	38.0	38.0	36.0	38.0
54-55	36.9080135550822	38.0	38.0	38.0	36.0	38.0
56-57	36.90931007363291	38.0	38.0	38.0	36.0	38.0
58-59	36.89334006397576	38.0	38.0	38.0	35.0	38.0
60-61	36.90049900932197	38.0	38.0	38.0	35.5	38.0
62-63	36.88783201038606	38.0	38.0	38.0	35.0	38.0
64-65	36.87941975659213	38.0	38.0	38.0	35.0	38.0
66-67	36.866979916483444	38.0	38.0	38.0	35.0	38.0
68-69	36.870525765651344	38.0	38.0	38.0	35.0	38.0
70-71	36.847334753224615	38.0	38.0	38.0	35.0	38.0
72-73	36.83259218706168	38.0	38.0	38.0	35.0	38.0
74-75	36.8450659063121	38.0	38.0	38.0	35.0	38.0
76-77	36.82399882953102	38.0	38.0	38.0	35.0	38.0
78-79	36.79838049517091	38.0	38.0	38.0	35.0	38.0
80-81	36.78820948998546	38.0	38.0	38.0	35.0	38.0
82-83	36.73350044374688	38.0	38.0	38.0	35.0	38.0
84-85	36.73381721271964	38.0	38.0	38.0	35.0	38.0
86-87	36.720866314669024	38.0	38.0	38.0	35.0	38.0
88-89	36.69448109868724	38.0	38.0	38.0	34.5	38.0
90-91	36.67289677873938	38.0	38.0	38.0	34.0	38.0
92-93	36.67128370640249	38.0	38.0	38.0	34.0	38.0
94-95	36.61708274514537	38.0	38.0	38.0	34.0	38.0
96-97	36.580813668518154	38.0	38.0	38.0	34.0	38.0
98-99	36.60534086922654	38.0	38.0	38.0	34.0	38.0
100	34.97464947265108	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	8.0
18	27.0
19	61.0
20	163.0
21	311.0
22	982.0
23	2694.0
24	6809.0
25	13726.0
26	24163.0
27	39489.0
28	60178.0
29	86910.0
30	118670.0
31	157219.0
32	207261.0
33	278625.0
34	407047.0
35	696000.0
36	1855381.0
37	7496478.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.38405906567139	21.831260049377406	14.31444363276163	24.470237252189577
2	13.821273738993087	21.624086244750927	41.295917202747766	23.25872281350822
3	18.581806363527296	25.457165355623314	30.292113254726033	25.66891502612336
4	12.837662136941	17.815752813301756	38.951626944757	30.394958105000242
5	13.426113669665327	37.79133025608246	35.32970420318937	13.45285187106284
6	30.30726688702619	38.067325789276715	18.444046616744355	13.181360706952747
7	26.730475064970037	32.64237742226342	22.7656829664723	17.861464546294243
8	24.222040442527994	37.339116093132134	20.76849500209654	17.670348462243332
9	25.011504337768404	17.256733683181626	21.356661365211686	36.37510061383828
10-11	24.12705434291152	27.07939486222824	28.777286673776796	20.016264121083438
12-13	24.835765209171125	24.35862552895941	29.098500008993906	21.70710925287556
14-15	21.81394896806745	25.993337351192373	26.74434575988094	25.448367920859237
16-17	21.534373913418573	29.140033506220025	27.417884350974596	21.907708229386802
18-19	21.50050269808374	28.289987375353665	29.21640309872285	20.993106827839746
20-21	22.264635787010693	27.362164622243583	29.20472572200835	21.168473868737376
22-23	21.919149174730855	27.316258484142647	29.072240019742118	21.692352321384377
24-25	21.751063844001305	27.38670258420531	29.15306546926789	21.709168102525496
26-27	21.74218682348529	27.569811023152358	29.02185462786661	21.66614752549574
28-29	21.628368065457884	27.632502033415857	29.06937934963278	21.66975055149348
30-31	21.93349834560754	27.434634163871017	29.009727878386265	21.62213961213518
32-33	21.68802330957434	27.53335077233264	29.021941475646777	21.756684442446243
34-35	21.86636540300942	27.611296140562708	28.978320367615872	21.544018088812
36-37	21.8293810359584	27.526540411093425	28.963284441419713	21.680794111528463
38-39	21.671035040463966	27.657179429309853	29.01016426776947	21.661621262456705
40-41	21.98887860647831	27.501215673101647	28.85942026623041	21.65048545418963
42-43	21.841211831094	27.55237708038947	28.74171704149349	21.864694047023036
44-45	21.93532914786228	27.622624465422934	28.61489154143192	21.827154845282863
46-47	21.918630743336156	27.69836418501603	28.433965547875246	21.94903952377257
48-49	21.87835702965362	27.74868641832547	28.461925904800378	21.91103064722053
50-51	21.92264081664256	27.864055091330115	28.30891137300904	21.904392719018286
52-53	22.053581821617797	27.83032311497654	28.18398643668734	21.932108626718318
54-55	22.01316769411005	27.760337433641645	28.26446271697695	21.962032155271356
56-57	22.014342676588196	27.818672054417444	28.251571117762488	21.915414151231875
58-59	21.96200029936764	27.90537602883829	28.25071016524475	21.88191350654932
60-61	22.06282797664532	27.84897597789956	28.27717583827501	21.81102020718011
62-63	22.023498448375303	27.841247449210215	28.259961314532383	21.8752927878821
64-65	21.989618534239153	27.854766665497365	28.221630749562486	21.933984050700996
66-67	22.03881500967828	27.81733353645743	28.12382263886788	22.020028814996415
68-69	22.027075876146462	27.82802842031321	28.162105766677527	21.9827899368628
70-71	21.9999700003	27.642323576764234	28.205167948320515	22.152538474615255
72-73	21.998863251724476	27.698986554968297	28.21647988430164	22.085670309005586
74-75	21.960927498533845	27.805705646552752	28.104388977274443	22.12897787763896
76-77	22.043080041117342	27.760865590247086	28.05322844812814	22.142825920507427
78-79	22.00775541218917	27.762400903469175	28.03466978040173	22.195173903939917
80-81	22.04830509457848	27.791401257074323	28.051357102367486	22.108936545979713
82-83	22.112054342985022	27.753765154025228	28.079709332636032	22.054471170353718
84-85	22.20459870533505	27.757794897782507	27.99682478633866	22.040781610543778
86-87	22.032825261564874	27.810136180292673	28.05519958358665	22.101838974555807
88-89	22.096200392766438	27.788819306560903	28.017066333495745	22.097913967176915
90-91	22.146077327051806	27.80314561414387	28.006533205348124	22.0442438534562
92-93	22.13421990561199	27.799464796340462	28.02734218832128	22.038973109726268
94-95	22.050956903721396	27.861353277139596	28.035357125659772	22.052332693479244
96-97	22.171413760128956	27.987217076065452	28.045258288506624	21.796110875298965
98-99	23.15188403281065	29.875655866454597	28.329735254357796	18.642724846376954
100	22.080483675811536	30.80216236426802	22.227107967616465	24.890245992303978
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1264.0
1	1287.0
2	1870.5
3	2691.0
4	3581.5
5	5129.5
6	6577.0
7	8538.5
8	10713.0
9	11832.5
10	12003.0
11	12182.0
12	13766.5
13	17489.5
14	24842.0
15	33628.5
16	39790.0
17	42563.5
18	42247.5
19	39580.5
20	36322.5
21	34169.0
22	34880.0
23	40203.5
24	50088.5
25	64848.0
26	84330.5
27	106670.5
28	131212.5
29	159109.0
30	187734.5
31	216957.0
32	247323.5
33	276038.0
34	305700.5
35	333714.5
36	360337.5
37	387203.0
38	409795.0
39	427175.5
40	445149.5
41	460921.5
42	470190.5
43	480305.5
44	487228.0
45	492532.5
46	495990.0
47	495480.0
48	491682.5
49	478214.5
50	461616.5
51	443131.5
52	419953.0
53	394576.0
54	367148.0
55	336254.5
56	305346.0
57	273665.0
58	239291.0
59	203928.5
60	167903.0
61	135308.5
62	106470.5
63	81768.0
64	61863.5
65	46117.5
66	33920.0
67	25596.5
68	19541.5
69	14153.5
70	10146.0
71	7316.0
72	5240.5
73	3576.0
74	2352.5
75	1518.5
76	971.0
77	601.5
78	356.5
79	219.5
80	134.0
81	83.5
82	60.0
83	37.5
84	22.0
85	12.5
86	11.5
87	12.0
88	8.0
89	4.0
90	2.0
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003676148918784353
3	0.0
4	0.0
5	0.0036848808639596124
6	6.985556140207796E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	8.732359215376568E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.313837042689456E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	4.5488076369749465E-6
48-49	2.751068847561212E-4
50-51	0.0014690657711833048
52-53	0.0037868799774908595
54-55	0.005285241385126825
56-57	0.005949239951544857
58-59	0.007471734120482902
60-61	0.007224415184550229
62-63	0.005114612767736442
64-65	0.003015737521509054
66-67	9.284931558264026E-4
68-69	4.1629402569505486E-4
70-71	1.3999840401819418E-4
72-73	4.2871234884233033E-4
74-75	5.085962428368034E-6
76-77	2.1034731265660294E-4
78-79	1.5532753243678972E-4
80-81	4.181117217516665E-5
82-83	2.6375575963452314E-5
84-85	1.0645065982643112E-4
86-87	1.5576267097101588E-4
88-89	3.2536212397695984E-5
90-91	5.477568071518635E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	99.0
20-21	337.0
22-23	560.0
24-25	854.0
26-27	2249.0
28-29	6471.0
30-31	19260.0
32-33	23845.0
34-35	34168.0
36-37	55736.0
38-39	66163.0
40-41	65589.0
42-43	74312.0
44-45	87994.0
46-47	88490.0
48-49	83373.0
50-51	76898.0
52-53	74926.0
54-55	80478.0
56-57	83158.0
58-59	83633.0
60-61	80878.0
62-63	79523.0
64-65	83395.0
66-67	88640.0
68-69	88850.0
70-71	88024.0
72-73	82829.0
74-75	83953.0
76-77	87996.0
78-79	90057.0
80-81	89539.0
82-83	84956.0
84-85	84418.0
86-87	87419.0
88-89	91371.0
90-91	93053.0
92-93	92364.0
94-95	111304.0
96-97	354055.0
98-99	798305.0
100-101	7702680.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	81.17289729654827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.42033841154738	70.96162151510029
2	8.271572730587476	13.428550474818133
3	2.293245535796098	5.584481530588334
4	0.9011229833267788	2.9258705350857515
5	0.42710051136876925	1.7334492972320175
6	0.21872783383156694	1.0652863194903754
7	0.14102330499171703	0.8013089176758714
8	0.08433181556505374	0.5476366242954847
9	0.0576174936319162	0.4209281003761252
>10	0.18082253260884296	2.2211575921524562
>50	0.003145263563668922	0.1713764015805464
>100	9.315824954376166E-4	0.12961442372106163
>500	2.0000685283064833E-5	0.008718267883480868
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.731945175259745E-6	0.0	0.0	0.0
3	0.0	8.731945175259745E-6	0.0	0.0	0.0
4	0.0	1.746389035051949E-5	0.0	1.746389035051949E-5	0.0
5	0.0	1.746389035051949E-5	0.0	2.6195835525779236E-5	0.0
6	0.0	1.746389035051949E-5	0.0	2.6195835525779236E-5	0.0
7	0.0	1.746389035051949E-5	0.0	2.6195835525779236E-5	0.0
8	0.0	1.746389035051949E-5	0.0	2.6195835525779236E-5	0.0
9	0.0	3.492778070103898E-5	0.0	5.239167105155847E-5	0.0
10-11	0.0	3.492778070103898E-5	0.0	7.85875065773377E-5	0.0
12-13	0.0	3.492778070103898E-5	0.0	7.85875065773377E-5	0.0
14-15	0.0	6.985556140207796E-5	0.0	8.731945175259744E-5	0.0
16-17	0.0	6.985556140207796E-5	0.0	8.731945175259744E-5	0.0
18-19	0.0	7.858750657733769E-5	0.0	1.0041736951548707E-4	0.0
20-21	0.0	8.731945175259744E-5	0.0	1.0478334210311694E-4	0.0
22-23	0.0	1.0041736951548707E-4	0.0	1.0478334210311694E-4	0.0
24-25	4.365972587629872E-6	1.1351528727837668E-4	0.0	1.2224723245363641E-4	0.0
26-27	8.731945175259745E-6	1.2224723245363641E-4	0.0	1.6154098574230527E-4	0.0
28-29	8.731945175259745E-6	1.2661320504126628E-4	0.0	3.318139166598703E-4	0.0
30-31	8.731945175259745E-6	1.4844306797941566E-4	0.0	9.736118870414615E-4	0.0
32-33	8.731945175259745E-6	1.4844306797941566E-4	0.0	0.002300867553680943	0.0
34-35	8.731945175259745E-6	1.6590695832993516E-4	0.0	0.004221895492238087	0.0
36-37	8.731945175259745E-6	1.8337084868045464E-4	0.0	0.006885138770692309	0.0
38-39	8.731945175259745E-6	1.9210279385571439E-4	0.0	0.011002250920827277	0.0
40-41	8.731945175259745E-6	2.0083473903097413E-4	0.0	0.015665109644415982	0.0
42-43	8.731945175259745E-6	2.05200711618604E-4	0.0	0.020022350286870598	0.0
44-45	8.731945175259745E-6	2.40128492319643E-4	0.0	0.02509124446110888	0.0
46-47	8.731945175259745E-6	2.706903004330521E-4	0.0	0.03050941644235755	0.0
48-49	8.731945175259745E-6	2.706903004330521E-4	0.0	0.03593632036878148	0.0
50-51	8.731945175259745E-6	3.1435002630935077E-4	0.0	0.04162518265046321	0.0
52-53	8.731945175259745E-6	3.2308197148461055E-4	0.0	0.04724855534333048	0.0
54-55	8.731945175259745E-6	3.2744794407224044E-4	0.0	0.05229998562721824	0.0
56-57	8.731945175259745E-6	3.4054586183513005E-4	0.0	0.057098189501023466	0.0
58-59	8.731945175259745E-6	3.4054586183513005E-4	0.0	0.061861465594127654	0.0
60-61	8.731945175259745E-6	3.4054586183513005E-4	0.0	0.06662910765981948	0.0
62-63	8.731945175259745E-6	3.4927780701038977E-4	0.0	0.0711784510961298	0.0
64-65	8.731945175259745E-6	3.8420558771142877E-4	0.0	0.07558371743704835	0.0
66-67	8.731945175259745E-6	4.0166947806194827E-4	0.0	0.08043867895449278	0.0
68-69	8.731945175259745E-6	4.147673958248379E-4	0.0	0.0848133834872979	0.0
70-71	8.731945175259745E-6	4.278653135877275E-4	0.0	0.08921864982821645	0.0
72-73	8.731945175259745E-6	4.322312861753573E-4	0.0	0.09285550499371212	0.0
74-75	8.731945175259745E-6	4.409632313506171E-4	0.0	0.09531791353313537	0.0
76-77	8.731945175259745E-6	4.6279309428876644E-4	0.0	0.0980553783455793	0.0
78-79	8.731945175259745E-6	4.715250394640262E-4	0.0	0.09979740140804362	0.0
80-81	8.731945175259745E-6	4.715250394640262E-4	0.0	0.10035624589926025	0.0
82-83	8.731945175259745E-6	4.758910120516561E-4	0.0	0.10072735356920878	0.0
84-85	8.731945175259745E-6	4.889889298145457E-4	0.0	0.10088016260977584	0.0
86-87	8.731945175259745E-6	4.889889298145457E-4	0.0	0.10095875011635316	0.0
88	8.731945175259745E-6	5.239167105155847E-4	0.0	0.10096748206152843	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12805	0.0	45.40189	1
GTATCAA	22930	0.0	35.818398	1
TCAACGC	26525	0.0	30.541676	4
ATCAACG	26865	0.0	30.13914	3
CAACGCA	27385	0.0	29.56684	5
AACGCAG	28400	0.0	28.509142	6
TATCAAC	28785	0.0	28.383383	2
ACGCAGA	33565	0.0	24.160572	7
CGCAGAG	33910	0.0	23.92744	8
GTGGTAT	7015	0.0	22.9869	1
TGGTATC	7285	0.0	20.718313	2
GCAGAGT	40865	0.0	19.865644	9
GAGTACT	24650	0.0	18.568676	12-13
GTACATG	28985	0.0	18.544432	1
TACATGG	29145	0.0	18.486889	2
AGAGTAC	36505	0.0	18.103966	10-11
ACATGGG	29555	0.0	17.589872	3
CAGAGTA	39630	0.0	17.457584	10-11
GTACTTT	27495	0.0	16.60054	14-15
CATGGGG	21760	0.0	16.105198	4
>>END_MODULE