FastQCFastQC Report
Fri 27 May 2016
SRR2139790_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139790_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913502
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56000.047.874931
GTATCAA102350.034.7149541
TCAACGC123300.030.9167184
ATCAACG124300.030.5637973
CAACGCA127800.029.8281025
TATCAAC129600.029.4477352
AACGCAG132750.028.713536
ACGCAGA157500.024.2014057
CGCAGAG160100.023.8623038
GTGGTAT29250.021.5492231
TGGTATC30200.020.8713472
GCAGAGT188350.020.397879
GAGTACT106600.020.38931812-13
CAGAGTA182950.018.51061810-11
GTACTTT118200.018.42513714-15
AGAGTAC166850.017.6578110-11
TACATGG132200.017.4713692
ACATGGG133650.016.7323363
TATCCCG2851.2748387E-716.6626325
GTACATG130800.016.5361561
Produced by FastQC (version 0.11.5)