FastQCFastQC Report
Fri 27 May 2016
SRR2139789_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139789_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5475233
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131300.031.4020181
GTATCAA192200.027.6133371
ATCAACG193900.026.4789453
TCAACGC193800.026.4480064
CAACGCA197700.025.948135
AACGCAG203800.025.1922136
TATCAAC220650.023.7395552
ACGCAGA226350.022.7206387
CGCAGAG229650.022.394158
GTACATG161000.022.146341
TACATGG156700.021.7335742
GAGTACT129750.021.35033612-13
ACATGGG159200.020.577293
GCAGAGT261650.019.5562259
GTACTTT144750.019.01904914-15
CAGAGTA237800.018.77338410-11
AGAGTAC199900.018.3655810-11
CATGGGG116700.018.220334
AGTACTT140300.017.0033912-13
ACTTTTT174600.015.87969816-17
Produced by FastQC (version 0.11.5)