##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139789_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5475233
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.289471516554638	31.0	18.0	33.0	18.0	33.0
2	29.425312310909874	31.0	27.0	33.0	18.0	33.0
3	31.282234746904834	33.0	32.0	33.0	27.0	33.0
4	31.71874219782062	33.0	31.0	33.0	29.0	33.0
5	32.403282198218776	33.0	33.0	33.0	32.0	34.0
6	36.041395498602526	38.0	36.0	38.0	33.0	38.0
7	36.72891016692805	38.0	37.0	38.0	34.0	38.0
8	36.62594833863691	38.0	37.0	38.0	34.0	38.0
9	36.938357874450276	38.0	38.0	38.0	35.0	38.0
10-11	37.071916756784596	38.0	38.0	38.0	36.0	38.0
12-13	37.11527052821314	38.0	38.0	38.0	36.0	38.0
14-15	37.09866356372413	38.0	38.0	38.0	36.0	38.0
16-17	37.041654939616265	38.0	38.0	38.0	36.0	38.0
18-19	37.081909938809915	38.0	38.0	38.0	36.0	38.0
20-21	37.117965756544145	38.0	38.0	38.0	36.0	38.0
22-23	37.126410391423946	38.0	38.0	38.0	36.0	38.0
24-25	37.13165362363996	38.0	38.0	38.0	36.0	38.0
26-27	37.10371261735937	38.0	38.0	38.0	36.0	38.0
28-29	37.078273882790015	38.0	38.0	38.0	36.0	38.0
30-31	37.080845796443455	38.0	38.0	38.0	36.0	38.0
32-33	37.07419160161404	38.0	38.0	38.0	36.0	38.0
34-35	37.05356566672911	38.0	38.0	38.0	36.0	38.0
36-37	37.035881556277765	38.0	38.0	38.0	36.0	38.0
38-39	37.01680196184277	38.0	38.0	38.0	36.0	38.0
40-41	36.99342363983189	38.0	38.0	38.0	36.0	38.0
42-43	36.96637596054222	38.0	38.0	38.0	36.0	38.0
44-45	36.91546277366628	38.0	38.0	38.0	35.5	38.0
46-47	36.88729856664541	38.0	38.0	38.0	35.0	38.0
48-49	36.850855612156984	38.0	38.0	38.0	35.0	38.0
50-51	36.804127179900135	38.0	38.0	38.0	35.0	38.0
52-53	36.77226692330646	38.0	38.0	38.0	35.0	38.0
54-55	36.73680618539251	38.0	38.0	38.0	35.0	38.0
56-57	36.73234744176121	38.0	38.0	38.0	35.0	38.0
58-59	36.71012381637662	38.0	38.0	38.0	35.0	38.0
60-61	36.714791991083544	38.0	38.0	38.0	35.0	38.0
62-63	36.69681523344997	38.0	38.0	38.0	34.5	38.0
64-65	36.68525250790826	38.0	38.0	38.0	34.0	38.0
66-67	36.666936730816985	38.0	38.0	38.0	34.0	38.0
68-69	36.66810189054929	38.0	38.0	38.0	34.0	38.0
70-71	36.6385712630464	38.0	38.0	38.0	34.0	38.0
72-73	36.61943600326363	38.0	38.0	38.0	34.0	38.0
74-75	36.63217551334712	38.0	38.0	38.0	34.0	38.0
76-77	36.6035125678317	38.0	38.0	38.0	34.0	38.0
78-79	36.57068130681445	38.0	38.0	38.0	34.0	38.0
80-81	36.55619159912128	38.0	38.0	38.0	34.0	38.0
82-83	36.488059065224476	38.0	38.0	38.0	34.0	38.0
84-85	36.48362247860638	38.0	38.0	38.0	34.0	38.0
86-87	36.46279942162216	38.0	38.0	38.0	34.0	38.0
88-89	36.42930852855187	38.0	38.0	38.0	34.0	38.0
90-91	36.399428942382045	38.0	38.0	38.0	34.0	38.0
92-93	36.399292366258265	38.0	38.0	38.0	34.0	38.0
94-95	36.34063336440194	38.0	37.0	38.0	34.0	38.0
96-97	36.30207716235144	38.0	37.0	38.0	33.0	38.0
98-99	36.33147444113543	38.0	37.0	38.0	33.5	38.0
100	34.58304003203701	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	6.0
19	30.0
20	95.0
21	276.0
22	785.0
23	1991.0
24	4672.0
25	8854.0
26	14952.0
27	23650.0
28	35352.0
29	49186.0
30	67463.0
31	88411.0
32	116592.0
33	154925.0
34	224159.0
35	380395.0
36	986876.0
37	3316561.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.315404842131834	17.79157160982921	14.397012875981716	22.496010672057242
2	14.506205140877984	19.42153296303582	43.52393043579272	22.548331460293472
3	18.26462910345551	25.74416467755801	29.999965298280458	25.991240920706023
4	12.321064692589339	18.153437488413733	38.60180927460073	30.923688544396192
5	13.774988173710387	36.92299122023565	35.612473950199906	13.689546655854059
6	30.382778440357754	37.41039189221274	18.686904477072197	13.519925190357299
7	27.14662554086739	32.66054613566217	22.354646825075754	17.838181498394682
8	24.475013209483503	36.90257565294481	20.54805704159074	18.074354095980937
9	25.36675608143069	17.55508852317335	21.27447361600867	35.80368177938729
10-11	24.44196438763428	26.861706159354316	28.36821738910472	20.328112063906687
12-13	25.2767781754676	24.207234651018506	28.76080342151649	21.755183751997404
14-15	22.165138908243723	25.788016692622946	26.6595960391092	25.387248360024127
16-17	21.703724754727332	28.96593624417445	27.284108274478914	22.046230726619306
18-19	21.660795074839736	28.037939207336017	29.154467033640397	21.14679868418385
20-21	22.469321357580725	27.048337160686554	29.204695909435195	21.277645572297523
22-23	22.241066491899	26.915352575676792	29.084331059782855	21.759249872641355
24-25	22.007317230895023	27.04743997389939	29.113914685647334	21.83132810955825
26-27	21.90362531656763	27.180825878964765	29.17122244823629	21.74432635623132
28-29	21.783174330059687	27.36682207845307	29.10250652910448	21.747497062382763
30-31	22.117176248374655	27.156066072687935	28.975143858464325	21.751613820473086
32-33	21.85623654825544	27.204496747305758	29.074748988557058	21.86451771588175
34-35	22.058303920174545	27.28318985305462	28.9775879211634	21.68091830560744
36-37	21.983060712970552	27.212255471123402	28.97393984883615	21.830743967069896
38-39	21.870861029785736	27.341187756248953	29.06681132369975	21.72113989026556
40-41	22.176182681589864	27.250197922481362	28.84619123288288	21.727428163045897
42-43	21.997094056285285	27.297733324615315	28.68958551342252	22.01558710567688
44-45	22.13770742753461	27.337376219605964	28.63325125330636	21.891665099553066
46-47	22.158714282121714	27.43030562418536	28.356150798828768	22.054829294864163
48-49	22.068796503893953	27.481568642749888	28.361599974909797	22.088034878446365
50-51	22.03418002238582	27.62366248202928	28.279232025644983	22.062925469939916
52-53	22.2024561345688	27.605651687974557	28.094115959252473	22.097776218204164
54-55	22.145626048427406	27.486616375649447	28.139091540000035	22.228666035923112
56-57	22.15486862396481	27.60592151385438	28.120705892872145	22.118503969308666
58-59	22.211477934510214	27.626203041304255	28.1515624195828	22.010756604602737
60-61	22.280899234884636	27.530872813778057	28.223943305290405	21.964284646046902
62-63	22.212043803682707	27.562019403593542	28.206573363938876	22.019363428784875
64-65	22.11783009047914	27.58082055492131	28.15746842473426	22.143880929865293
66-67	22.186949051323705	27.576753201489513	28.04766718776433	22.18863055942246
68-69	22.183332746035923	27.509764119100627	28.096859486166476	22.210043648696974
70-71	22.193602705984123	27.381443055489807	28.137575600565285	22.287378637960785
72-73	22.211021701881297	27.39888620902545	28.156868920703797	22.233223168389458
74-75	22.085202407290073	27.56647097085192	28.05563332400112	22.29269329785689
76-77	22.2115552054199	27.468266273499786	27.97923606874577	22.34094245233455
78-79	22.20333369940104	27.426702434264623	27.994182306427785	22.375781559906553
80-81	22.23394921842264	27.547686024741957	27.936811589444883	22.281553167390516
82-83	22.2538496752291	27.523568250695114	28.001807587378526	22.22077448669726
84-85	22.39665939294619	27.481420802019997	27.943779738146063	22.17814006688775
86-87	22.224228956645558	27.55475862699009	27.96338962500564	22.257622791358713
88-89	22.279883480444884	27.55423627670944	27.881699955543716	22.284180287301965
90-91	22.335607798864814	27.556706095456146	27.89148096811063	22.216205137568405
92-93	22.272240616520957	27.54557424120142	27.970146123518024	22.2120390187596
94-95	22.185705957880135	27.583870316120368	27.99663836169365	22.23378536430585
96-97	22.31378613589696	27.764567329192257	27.966392181745388	21.955254353165397
98-99	23.358982274853357	29.656019861362502	28.188957473475845	18.796040390308292
100	22.20189489681243	30.609650666144884	22.060954598757654	25.127499838285033
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	641.0
1	614.5
2	907.0
3	1344.5
4	1767.5
5	2688.0
6	3579.5
7	4487.5
8	5729.0
9	6532.0
10	6485.0
11	6465.0
12	7240.5
13	8956.5
14	12280.5
15	16410.5
16	19939.5
17	22557.0
18	23437.5
19	22128.5
20	19844.0
21	17761.5
22	17145.0
23	18795.0
24	22949.5
25	29457.5
26	37823.0
27	47303.5
28	57467.0
29	70105.5
30	83207.0
31	95656.5
32	109194.0
33	122530.0
34	135124.0
35	147533.0
36	160809.0
37	174011.0
38	185268.0
39	193848.5
40	202664.5
41	211125.0
42	216815.5
43	222653.0
44	228574.5
45	234577.0
46	239839.5
47	242953.0
48	242811.0
49	236389.0
50	227272.0
51	216988.5
52	204505.5
53	193121.5
54	181302.5
55	166852.5
56	151132.0
57	135174.5
58	118954.5
59	101938.5
60	84615.0
61	68081.5
62	53379.0
63	41058.5
64	31088.0
65	23142.5
66	17015.5
67	12976.5
68	9913.5
69	7232.5
70	5185.5
71	3705.5
72	2676.0
73	1848.5
74	1185.5
75	718.0
76	435.0
77	258.0
78	151.5
79	88.5
80	57.5
81	34.0
82	17.5
83	9.5
84	8.0
85	8.0
86	6.5
87	4.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003214475073480891
3	0.0
4	0.0
5	0.0026117609972032244
6	5.479218875251519E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	9.13292469583936E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	3.25100699941807E-4
50-51	0.0014829927216835782
52-53	0.003790007968709849
54-55	0.0059707952016270225
56-57	0.006484494933988333
58-59	0.008313210086694904
60-61	0.007945641048238417
62-63	0.0056026695214175595
64-65	0.0032259121443558273
66-67	0.0010496576740217777
68-69	5.033952468393456E-4
70-71	2.3824602859408116E-4
72-73	5.116962804797372E-4
74-75	0.0
76-77	2.1223156288702416E-4
78-79	1.6055575410950482E-4
80-81	5.3997190634165685E-5
82-83	1.0894329686601728E-5
84-85	1.3187630530068017E-4
86-87	1.441298889689857E-4
88-89	2.237919737232416E-5
90-91	1.1298509444141582E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	104.0
20-21	359.0
22-23	456.0
24-25	692.0
26-27	1696.0
28-29	3024.0
30-31	8252.0
32-33	10766.0
34-35	14614.0
36-37	23974.0
38-39	28946.0
40-41	29157.0
42-43	33706.0
44-45	40338.0
46-47	40417.0
48-49	37676.0
50-51	34678.0
52-53	32669.0
54-55	35461.0
56-57	36798.0
58-59	37024.0
60-61	35852.0
62-63	35145.0
64-65	37315.0
66-67	39107.0
68-69	40051.0
70-71	39515.0
72-73	37541.0
74-75	38233.0
76-77	39975.0
78-79	41497.0
80-81	40711.0
82-83	39636.0
84-85	40178.0
86-87	40904.0
88-89	42307.0
90-91	43671.0
92-93	43966.0
94-95	53065.0
96-97	173461.0
98-99	396605.0
100-101	3725691.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.58200165327636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.51980953451628	76.61284487583958
2	7.435850348389633	12.727499136187944
3	1.7858519631378484	4.585103569853106
4	0.6394338937304923	2.188961302016158
5	0.26260115994203376	1.1236966452155712
6	0.13851744242205308	0.7112759991823057
7	0.07167067970968444	0.4293604160583958
8	0.04157906550651914	0.2846735722336486
9	0.027125192703705954	0.20892854581326029
>10	0.07556135691232128	0.9376571858025666
>50	0.0013850951677678528	0.07738257019871955
>100	5.823473580803337E-4	0.09393052873842768
>500	3.192050362038153E-5	0.018685652860312277
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.8264062917505065E-5	1.8264062917505065E-5
2	0.0	1.8264062917505065E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
3	0.0	1.8264062917505065E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
4	0.0	3.652812583501013E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
5	0.0	3.652812583501013E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
6	0.0	3.652812583501013E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
7	0.0	3.652812583501013E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
8	0.0	3.652812583501013E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
9	0.0	7.305625167002026E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
10-11	0.0	7.305625167002026E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
12-13	0.0	7.305625167002026E-5	0.0	1.8264062917505065E-5	1.8264062917505065E-5
14-15	0.0	1.1871640896378292E-4	0.0	2.7396094376257596E-5	5.479218875251519E-5
16-17	0.0	1.2784844042253545E-4	0.0	3.652812583501013E-5	5.479218875251519E-5
18-19	0.0	1.3698047188128798E-4	0.0	3.652812583501013E-5	5.479218875251519E-5
20-21	0.0	1.4611250334004052E-4	0.0	3.652812583501013E-5	5.479218875251519E-5
22-23	0.0	1.5524453479879305E-4	0.0	8.218828312877278E-5	6.392422021126773E-5
24-25	0.0	1.8264062917505063E-4	0.0	1.4611250334004052E-4	7.305625167002026E-5
26-27	0.0	1.8264062917505063E-4	0.0	2.009046920925557E-4	7.305625167002026E-5
28-29	0.0	1.8264062917505063E-4	0.0	3.0135703813883354E-4	7.305625167002026E-5
30-31	0.0	1.8264062917505063E-4	0.0	7.762226739939652E-4	7.305625167002026E-5
32-33	0.0	1.8264062917505063E-4	0.0	0.0021368953613480923	7.305625167002026E-5
34-35	0.0	2.1916875501006076E-4	0.0	0.003808057118299806	7.305625167002026E-5
36-37	0.0	2.556968808450709E-4	0.0	0.00649287436717305	7.305625167002026E-5
38-39	1.8264062917505065E-5	2.7396094376257597E-4	0.0	0.01017308304505032	7.305625167002026E-5
40-41	1.8264062917505065E-5	3.1048906959758605E-4	0.0	0.014483401893581514	7.305625167002026E-5
42-43	1.8264062917505065E-5	3.1048906959758605E-4	0.0	0.018601948081478907	7.305625167002026E-5
44-45	1.8264062917505065E-5	3.1048906959758605E-4	0.0	0.02262004192333002	7.305625167002026E-5
46-47	1.8264062917505065E-5	3.1048906959758605E-4	0.0	0.027478282659386365	7.305625167002026E-5
48-49	1.8264062917505065E-5	3.1048906959758605E-4	0.0	0.03251916402461777	7.305625167002026E-5
50-51	1.8264062917505065E-5	3.287531325150911E-4	0.0	0.03774268601902421	7.305625167002026E-5
52-53	1.8264062917505065E-5	3.470171954325962E-4	0.0	0.04266485097529183	7.305625167002026E-5
54-55	1.8264062917505065E-5	3.7441328980885376E-4	0.0	0.047550487805724434	7.305625167002026E-5
56-57	1.8264062917505065E-5	3.835453212676063E-4	0.0	0.05183341055987937	8.218828312877278E-5
58-59	1.8264062917505065E-5	3.835453212676063E-4	0.0	0.05604327706236428	9.132031458752531E-5
60-61	1.8264062917505065E-5	3.835453212676063E-4	0.0	0.06073714123216309	1.0045234604627785E-4
62-63	1.8264062917505065E-5	3.835453212676063E-4	0.0	0.06483742335714299	1.2784844042253545E-4
64-65	1.8264062917505065E-5	4.3833751002012153E-4	0.0	0.06886464923045285	1.2784844042253545E-4
66-67	1.8264062917505065E-5	4.3833751002012153E-4	0.0	0.0733941368339941	1.4611250334004052E-4
68-69	1.8264062917505065E-5	4.566015729376266E-4	0.0	0.07782317209148908	1.5524453479879305E-4
70-71	1.8264062917505065E-5	4.7486563585513166E-4	0.0	0.0817042854614589	1.6437656625754556E-4
72-73	1.8264062917505065E-5	4.7486563585513166E-4	0.0	0.08473611990576474	1.6437656625754556E-4
74-75	1.8264062917505065E-5	4.8399766731388417E-4	0.0	0.08734788090296797	1.6437656625754556E-4
76-77	1.8264062917505065E-5	5.113937616901417E-4	0.0	0.0894939082957748	2.009046920925557E-4
78-79	1.8264062917505065E-5	5.296578246076468E-4	0.0	0.09100069348646897	2.3743281792756583E-4
80-81	1.8264062917505065E-5	5.296578246076468E-4	0.0	0.09160340756274665	2.3743281792756583E-4
82-83	1.8264062917505065E-5	5.296578246076468E-4	0.0	0.09196868882109674	2.3743281792756583E-4
84-85	1.8264062917505065E-5	5.296578246076468E-4	0.0	0.09216046148173054	2.3743281792756583E-4
86-87	1.8264062917505065E-5	5.296578246076468E-4	0.0	0.09227917789069433	2.3743281792756583E-4
88	1.8264062917505065E-5	5.66185950442657E-4	0.0	0.09230657398507058	2.3743281792756583E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	13130	0.0	31.402018	1
GTATCAA	19220	0.0	27.613337	1
ATCAACG	19390	0.0	26.478945	3
TCAACGC	19380	0.0	26.448006	4
CAACGCA	19770	0.0	25.94813	5
AACGCAG	20380	0.0	25.192213	6
TATCAAC	22065	0.0	23.739555	2
ACGCAGA	22635	0.0	22.720638	7
CGCAGAG	22965	0.0	22.39415	8
GTACATG	16100	0.0	22.14634	1
TACATGG	15670	0.0	21.733574	2
GAGTACT	12975	0.0	21.350336	12-13
ACATGGG	15920	0.0	20.57729	3
GCAGAGT	26165	0.0	19.556225	9
GTACTTT	14475	0.0	19.019049	14-15
CAGAGTA	23780	0.0	18.773384	10-11
AGAGTAC	19990	0.0	18.36558	10-11
CATGGGG	11670	0.0	18.22033	4
AGTACTT	14030	0.0	17.00339	12-13
ACTTTTT	17460	0.0	15.879698	16-17
>>END_MODULE