FastQCFastQC Report
Fri 27 May 2016
SRR2139788_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139788_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6682101
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94000.047.339011
GTATCAA158400.032.942721
TCAACGC180500.028.8593064
ATCAACG182900.028.4570183
CAACGCA188300.027.7096985
AACGCAG193050.027.0941586
TATCAAC197750.026.5839272
ACGCAGA223650.023.4064047
CGCAGAG227800.022.9231598
GTGGTAT48900.020.1241
GAGTACT147850.020.00916512-13
TGGTATC47500.019.7176172
GTACATG189800.019.6701761
GCAGAGT268550.019.4608529
TACATGG193550.019.1998712
CAGAGTA259650.018.52399310-11
ACATGGG197050.018.353693
GTACTTT164900.017.74427814-15
AGAGTAC236950.017.4482410-11
CATGGGG146100.016.6012924
Produced by FastQC (version 0.11.5)