##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139788_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6682101
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.981098609554092	32.0	25.0	33.0	18.0	33.0
2	28.250389810031304	31.0	25.0	33.0	18.0	33.0
3	31.166396467218917	33.0	31.0	33.0	27.0	33.0
4	31.471011138562556	33.0	31.0	33.0	29.0	34.0
5	32.57324440321989	33.0	33.0	33.0	32.0	34.0
6	36.53291981070026	38.0	37.0	38.0	34.0	38.0
7	36.77172838303402	38.0	37.0	38.0	34.0	38.0
8	37.01970996846651	38.0	38.0	38.0	35.0	38.0
9	37.0989860823714	38.0	38.0	38.0	36.0	38.0
10-11	37.17696657084351	38.0	38.0	38.0	36.0	38.0
12-13	37.201640995848464	38.0	38.0	38.0	36.0	38.0
14-15	37.20777948432686	38.0	38.0	38.0	36.0	38.0
16-17	37.18049098629308	38.0	38.0	38.0	36.0	38.0
18-19	37.20109640665413	38.0	38.0	38.0	36.0	38.0
20-21	37.22315386491932	38.0	38.0	38.0	36.0	38.0
22-23	37.23215601951871	38.0	38.0	38.0	36.0	38.0
24-25	37.23698664831557	38.0	38.0	38.0	36.0	38.0
26-27	37.208158459627	38.0	38.0	38.0	36.0	38.0
28-29	37.17941360300931	38.0	38.0	38.0	36.0	38.0
30-31	37.182454351237396	38.0	38.0	38.0	36.0	38.0
32-33	37.17476503046945	38.0	38.0	38.0	36.0	38.0
34-35	37.15669725034661	38.0	38.0	38.0	36.0	38.0
36-37	37.14232858164351	38.0	38.0	38.0	36.0	38.0
38-39	37.129541847741635	38.0	38.0	38.0	36.0	38.0
40-41	37.11253694119385	38.0	38.0	38.0	36.0	38.0
42-43	37.09337641470195	38.0	38.0	38.0	36.0	38.0
44-45	37.05081124867695	38.0	38.0	38.0	36.0	38.0
46-47	37.034126973152574	38.0	38.0	38.0	36.0	38.0
48-49	37.00867033610396	38.0	38.0	38.0	36.0	38.0
50-51	36.973629331638136	38.0	38.0	38.0	36.0	38.0
52-53	36.95534489210422	38.0	38.0	38.0	36.0	38.0
54-55	36.931299014590095	38.0	38.0	38.0	36.0	38.0
56-57	36.93894312665627	38.0	38.0	38.0	36.0	38.0
58-59	36.92665079755738	38.0	38.0	38.0	36.0	38.0
60-61	36.94003822658307	38.0	38.0	38.0	36.0	38.0
62-63	36.93283058309304	38.0	38.0	38.0	36.0	38.0
64-65	36.926893533704224	38.0	38.0	38.0	36.0	38.0
66-67	36.91925955229311	38.0	38.0	38.0	35.5	38.0
68-69	36.9260761659648	38.0	38.0	38.0	36.0	38.0
70-71	36.90923315739302	38.0	38.0	38.0	35.5	38.0
72-73	36.89630999451184	38.0	38.0	38.0	35.0	38.0
74-75	36.911714548359214	38.0	38.0	38.0	35.0	38.0
76-77	36.893668575420406	38.0	38.0	38.0	35.0	38.0
78-79	36.872128031184204	38.0	38.0	38.0	35.0	38.0
80-81	36.86518722902291	38.0	38.0	38.0	35.0	38.0
82-83	36.81366160624445	38.0	38.0	38.0	35.0	38.0
84-85	36.81780403069923	38.0	38.0	38.0	35.0	38.0
86-87	36.80944970676735	38.0	38.0	38.0	35.0	38.0
88-89	36.78716248370179	38.0	38.0	38.0	35.0	38.0
90-91	36.76684230769996	38.0	38.0	38.0	35.0	38.0
92-93	36.767889566510476	38.0	38.0	38.0	35.0	38.0
94-95	36.71564707281066	38.0	38.0	38.0	34.5	38.0
96-97	36.6811846525664	38.0	38.0	38.0	34.0	38.0
98-99	36.70497558176733	38.0	38.0	38.0	34.0	38.0
100	35.171468514132364	38.0	35.0	38.0	29.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	18.0
19	42.0
20	89.0
21	179.0
22	503.0
23	1480.0
24	3609.0
25	7455.0
26	13334.0
27	21725.0
28	33606.0
29	48465.0
30	67209.0
31	90119.0
32	118928.0
33	159994.0
34	236424.0
35	417832.0
36	1146518.0
37	4314570.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.14017447506406	21.397132428857333	14.539139710698775	23.923553385379837
2	15.26442251929675	20.08901759624055	41.573357578206554	23.07320230625615
3	18.28040312470584	25.518246431773477	30.010755599174573	26.19059484434611
4	12.812901211759595	17.811284205371933	38.37135954694489	31.004455035923584
5	13.642293478864381	37.08979411453506	35.62346385821199	13.644448548388569
6	30.722794381521723	37.528804593290666	18.32042470518972	13.427976319997892
7	27.247014075363424	32.467767248654276	22.35394227055233	17.93127640542997
8	24.563801115846648	37.15464642033995	20.47579047368485	17.805761990128556
9	25.32209555048629	17.324910234071588	21.113015202853113	36.239979012589004
10-11	24.46289722349303	26.895081352406976	28.411378397303483	20.230643026796514
12-13	25.153480918651184	24.195264333777654	28.77793975278135	21.873314994789812
14-15	22.193985095406372	25.788842461375545	26.36111007600753	25.656062367210552
16-17	21.855977633382075	29.014332468186275	27.080823830708333	22.04886606772331
18-19	21.787151975104834	28.07586266654754	28.89442257756954	21.242562780778083
20-21	22.6041438830273	27.118464099544305	28.912396262808443	21.36499575461995
22-23	22.358641110823843	26.976884276960643	28.78625710568743	21.878217506528085
24-25	22.08649794239607	27.15075550247219	28.832692366697277	21.93005418843446
26-27	22.047003950924417	27.28906262278813	28.80124357946717	21.862689846820288
28-29	21.98688789580628	27.285181753132683	28.840191008444993	21.887739342616044
30-31	22.246650059000917	27.13921594335912	28.75461986551537	21.859514132124595
32-33	22.01764717448435	27.293533056669713	28.736583643065206	21.952236125780733
34-35	22.175218346769615	27.2811654748182	28.70305756929702	21.84055860911517
36-37	22.138070329741648	27.219021939888023	28.681844645504427	21.961063084865902
38-39	22.00999996738828	27.31462264862849	28.8025665880168	21.87281079596643
40-41	22.289427866103235	27.179963530954076	28.64018962073565	21.89041898220704
42-43	22.14448217088318	27.253503670862933	28.52221537774364	22.079798780510252
44-45	22.222658042773144	27.363121118923623	28.364595565048894	22.04962527325434
46-47	22.274454468370397	27.42663171213824	28.122806896046747	22.176106923444618
48-49	22.158023310929885	27.460940333224993	28.195843489245288	22.185192866599834
50-51	22.205021293552832	27.577620759087694	28.073563466017227	22.143794481342248
52-53	22.36151348532213	27.48335204307162	27.963447637643778	22.191686833962468
54-55	22.29849996445869	27.47068962315321	27.9861987636746	22.2446116487135
56-57	22.253184167647376	27.547491364729908	27.99762049260029	22.20170397502243
58-59	22.270469291563273	27.557246121453748	27.994914868492586	22.17736971849039
60-61	22.329557173253555	27.523018408422175	28.05314205890803	22.094282359416237
62-63	22.327624356559536	27.518708800941656	28.01106628926683	22.14260055323198
64-65	22.300791416406017	27.546343230747244	27.960191522105482	22.192673830741256
66-67	22.29839512535861	27.545437998102056	27.862253167167456	22.29391370937188
68-69	22.27878928153293	27.50187105199582	27.95604252651994	22.26329713995131
70-71	22.29666678679351	27.348068722437386	27.975889094126877	22.379375396642224
72-73	22.341398853572215	27.39048164605614	27.879694496686174	22.388425003685462
74-75	22.22900208586171	27.520713561114814	27.834926466576594	22.415357886446888
76-77	22.398371587662734	27.44357688349578	27.778410874594872	22.379640654246614
78-79	22.30303540712209	27.47264296231407	27.75826014367367	22.466061486890172
80-81	22.34800724156202	27.51740235317877	27.726219035829057	22.408371369430153
82-83	22.40236859178275	27.53390191828814	27.754417074265753	22.309312415663356
84-85	22.481001684387213	27.47467019398087	27.72574076719084	22.318587354441075
86-87	22.305486900823027	27.50872331031115	27.838028787813673	22.347761001052152
88-89	22.406693604847806	27.484108876514675	27.751409595071824	22.3577879235657
90-91	22.381380815438646	27.591679877541676	27.722522627390845	22.304416679628837
92-93	22.439682462194753	27.47548807918918	27.75466674677712	22.33016271183895
94-95	22.331821163811032	27.55532088131053	27.755603211117492	22.357254743760947
96-97	22.460360497189445	27.697689321468577	27.77538118775002	22.06656899359196
98-99	23.559297697818675	29.57774887636787	27.96962073432293	18.893332691490528
100	22.370990985011503	30.495469429279293	21.969702131101172	25.16383745460803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	718.0
1	734.5
2	1029.5
3	1440.0
4	1868.5
5	2654.5
6	3463.0
7	4467.0
8	5748.5
9	6654.5
10	6904.0
11	7221.0
12	8253.0
13	10216.0
14	13861.5
15	18207.5
16	21570.5
17	23448.0
18	23542.5
19	22235.5
20	20415.5
21	18605.5
22	18457.5
23	20774.0
24	25634.5
25	33301.5
26	43107.0
27	54899.0
28	68071.0
29	83204.5
30	99512.5
31	116292.0
32	134302.5
33	152138.5
34	169861.0
35	186372.0
36	203327.0
37	220258.5
38	233870.5
39	244785.5
40	255534.0
41	264504.5
42	270647.0
43	275975.0
44	279246.5
45	282533.5
46	283982.0
47	281756.0
48	278929.0
49	274375.5
50	267274.0
51	257934.5
52	247364.0
53	235148.5
54	221881.5
55	207584.0
56	190480.0
57	172245.0
58	153923.0
59	133092.0
60	110376.0
61	90088.0
62	71822.0
63	55582.5
64	41971.5
65	31173.5
66	23236.5
67	17831.5
68	13695.5
69	10140.5
70	7619.0
71	5619.0
72	3964.5
73	2684.0
74	1786.0
75	1195.5
76	778.5
77	485.0
78	301.0
79	189.5
80	124.5
81	79.5
82	49.5
83	30.0
84	23.0
85	18.0
86	12.5
87	10.0
88	6.0
89	2.5
90	1.5
91	1.5
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004130437417812152
3	0.0
4	0.0
5	0.0027236942392819268
6	1.0475747074161255E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	7.483116592344353E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.001882931068513E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	2.3343821971921117E-5
48-49	4.391020019052322E-4
50-51	0.0018005239050741687
52-53	0.004348632490231278
54-55	0.006123297273115627
56-57	0.00703126121312801
58-59	0.008555198416418962
60-61	0.008236191709397028
62-63	0.0060870783780625
64-65	0.003672755186271009
66-67	0.001189445796159313
68-69	4.6459314522379237E-4
70-71	1.9595120508287205E-4
72-73	6.44307368290473E-4
74-75	0.0
76-77	3.230966420478669E-4
78-79	2.026149485256723E-4
80-81	7.111068760746046E-5
82-83	0.0
84-85	9.95018575187674E-5
86-87	1.0949463572092773E-4
88-89	9.208374464072606E-6
90-91	9.297430376192628E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	74.0
20-21	234.0
22-23	389.0
24-25	482.0
26-27	1354.0
28-29	3454.0
30-31	10584.0
32-33	13575.0
34-35	19033.0
36-37	30894.0
38-39	36821.0
40-41	36166.0
42-43	41376.0
44-45	49332.0
46-47	49847.0
48-49	46200.0
50-51	42797.0
52-53	41743.0
54-55	45142.0
56-57	46677.0
58-59	47129.0
60-61	45638.0
62-63	44375.0
64-65	47140.0
66-67	50051.0
68-69	50320.0
70-71	49386.0
72-73	46618.0
74-75	47147.0
76-77	49677.0
78-79	50770.0
80-81	50386.0
82-83	47885.0
84-85	47653.0
86-87	49131.0
88-89	51527.0
90-91	52430.0
92-93	51598.0
94-95	62970.0
96-97	207029.0
98-99	466978.0
100-101	4550089.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.92538440348176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.8328067430119	77.18918451436986
2	7.275946950052313	12.503770771651713
3	1.6898146430972105	4.355939183362803
4	0.5887758973961131	2.0236318124506383
5	0.24883383745546583	1.0690571567977198
6	0.13186154140216816	0.6798152179810154
7	0.07561233957950106	0.4547913540810658
8	0.043926428529002484	0.3019516205461285
9	0.027259142383955083	0.21080270590655242
>10	0.08334473390412725	1.0596104954307106
>50	0.0014656948584332364	0.0829581736582617
>100	3.257163850430775E-4	0.05083885041752259
>500	2.6331944814305324E-5	0.017648143346031535
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	5.986141185235003E-5	0.0	0.0	0.0
3	0.0	5.986141185235003E-5	0.0	1.4965352963087507E-5	0.0
4	0.0	7.482676481543754E-5	0.0	2.9930705926175015E-5	0.0
5	0.0	7.482676481543754E-5	0.0	2.9930705926175015E-5	0.0
6	0.0	7.482676481543754E-5	0.0	2.9930705926175015E-5	0.0
7	0.0	8.979211777852505E-5	0.0	2.9930705926175015E-5	0.0
8	0.0	8.979211777852505E-5	0.0	2.9930705926175015E-5	1.4965352963087507E-5
9	0.0	1.795842355570501E-4	0.0	4.4896058889262524E-5	1.4965352963087507E-5
10-11	0.0	1.795842355570501E-4	0.0	5.986141185235003E-5	1.4965352963087507E-5
12-13	0.0	1.795842355570501E-4	0.0	5.986141185235003E-5	1.4965352963087507E-5
14-15	0.0	2.2448029444631262E-4	0.0	8.979211777852505E-5	2.2448029444631262E-5
16-17	0.0	2.3944564740940012E-4	0.0	1.1972282370470006E-4	4.4896058889262524E-5
18-19	0.0	2.5441100037248765E-4	0.0	1.496535296308751E-4	4.4896058889262524E-5
20-21	0.0	2.6937635333557514E-4	0.0	1.8706691203859385E-4	5.2378735370806274E-5
22-23	0.0	3.3672044166946894E-4	0.0	2.0951494148322512E-4	1.122401472231563E-4
24-25	0.0	3.591684711141002E-4	0.0	2.1699761796476887E-4	1.646188825939626E-4
26-27	0.0	3.741338240771877E-4	0.0	2.768590298171189E-4	1.795842355570501E-4
28-29	0.0	3.890991770402752E-4	0.0	4.86373971300344E-4	1.795842355570501E-4
30-31	0.0	4.4896058889262524E-4	0.0	9.72747942600688E-4	1.795842355570501E-4
32-33	0.0	4.7889129481880024E-4	0.0	0.002087666738350707	2.2448029444631262E-4
34-35	0.0	5.836487655604128E-4	0.0	0.003584202034659458	2.2448029444631262E-4
36-37	0.0	6.884062363020254E-4	0.0	0.006390205715238366	2.2448029444631262E-4
38-39	0.0	6.884062363020254E-4	0.0	0.010124061279528699	2.2448029444631262E-4
40-41	0.0	7.781983540805504E-4	0.0	0.014261981373822395	2.2448029444631262E-4
42-43	0.0	8.081290600067254E-4	0.0	0.018774035292193278	2.2448029444631262E-4
44-45	0.0	9.278518837114256E-4	0.0	0.023682671064085982	2.2448029444631262E-4
46-47	1.4965352963087507E-5	0.0010176440014899506	0.0	0.029272230395799164	2.2448029444631262E-4
48-49	1.4965352963087507E-5	0.0010251266779714944	0.0	0.034599896050658316	2.3944564740940012E-4
50-51	1.4965352963087507E-5	0.0011149187957500194	0.0	0.039613289293292636	2.3944564740940012E-4
52-53	1.4965352963087507E-5	0.0011224014722315632	0.0	0.045045712418893394	2.768590298171189E-4
54-55	1.4965352963087507E-5	0.0011448495016761944	0.0	0.0498346253670814	3.217550887063814E-4
56-57	1.4965352963087507E-5	0.0011822628840839132	0.0	0.05408478560859825	3.442031181510127E-4
58-59	1.4965352963087507E-5	0.0011822628840839132	0.0	0.05878390643900773	3.591684711141002E-4
60-61	1.4965352963087507E-5	0.0011897455605654568	0.0	0.06308644541589539	3.591684711141002E-4
62-63	1.4965352963087507E-5	0.0012121935900100882	0.0	0.06733660565741224	3.591684711141002E-4
64-65	1.4965352963087507E-5	0.0013468817666778758	0.0	0.07171397139911534	3.6665114759564394E-4
66-67	1.4965352963087507E-5	0.0013543644431594196	0.0	0.07596413164063219	3.8161650055873144E-4
68-69	1.4965352963087507E-5	0.001369329796122507	0.0	0.08003470764659198	3.890991770402752E-4
70-71	1.4965352963087507E-5	0.0013768124726040508	0.0	0.08401549153477328	3.890991770402752E-4
72-73	1.4965352963087507E-5	0.0013842951490855944	0.0	0.08706094086276157	3.890991770402752E-4
74-75	1.4965352963087507E-5	0.0014217085314933132	0.0	0.08938057057204014	3.890991770402752E-4
76-77	1.4965352963087507E-5	0.0014890526198272072	0.0	0.09141585857502005	3.890991770402752E-4
78-79	1.4965352963087507E-5	0.0015264660022349258	0.0	0.09245595060595463	3.890991770402752E-4
80-81	1.4965352963087507E-5	0.0015264660022349258	0.0	0.09287498048892107	3.9658185352181894E-4
82-83	1.4965352963087507E-5	0.0015264660022349258	0.0	0.09342121587207376	4.26512559447994E-4
84-85	1.4965352963087507E-5	0.0015414313551980132	0.0	0.09360828278411237	4.3399523592953774E-4
86-87	1.4965352963087507E-5	0.001578844737605732	0.0	0.09366814419596471	4.414779124110815E-4
88	1.4965352963087507E-5	0.0016312234729765384	0.0	0.09366814419596471	4.4896058889262524E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9400	0.0	47.33901	1
GTATCAA	15840	0.0	32.94272	1
TCAACGC	18050	0.0	28.859306	4
ATCAACG	18290	0.0	28.457018	3
CAACGCA	18830	0.0	27.709698	5
AACGCAG	19305	0.0	27.094158	6
TATCAAC	19775	0.0	26.583927	2
ACGCAGA	22365	0.0	23.406404	7
CGCAGAG	22780	0.0	22.923159	8
GTGGTAT	4890	0.0	20.124	1
GAGTACT	14785	0.0	20.009165	12-13
TGGTATC	4750	0.0	19.717617	2
GTACATG	18980	0.0	19.670176	1
GCAGAGT	26855	0.0	19.460852	9
TACATGG	19355	0.0	19.199871	2
CAGAGTA	25965	0.0	18.523993	10-11
ACATGGG	19705	0.0	18.35369	3
GTACTTT	16490	0.0	17.744278	14-15
AGAGTAC	23695	0.0	17.44824	10-11
CATGGGG	14610	0.0	16.601292	4
>>END_MODULE