FastQCFastQC Report
Fri 27 May 2016
SRR2139787_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139787_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5265304
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73600.044.9087871
GTATCAA127600.033.4501881
TCAACGC154800.028.636894
ATCAACG156200.028.463533
CAACGCA160150.027.6531585
TATCAAC165750.027.1642672
AACGCAG163900.026.992976
ACGCAGA187050.023.6522187
CGCAGAG191850.023.0604528
TGGTATC37150.022.1844652
GTGGTAT39650.021.1138941
GCAGAGT221400.020.002199
GAGTACT122700.019.81346712-13
TACATGG159600.019.3509062
GTACATG155950.019.275341
AGAGTAC193150.019.0989310-11
CAGAGTA215050.018.47504210-11
ACATGGG160150.018.4715523
GTACTTT133450.018.20158414-15
AGTACTT134100.017.20728312-13
Produced by FastQC (version 0.11.5)