FastQCFastQC Report
Fri 27 May 2016
SRR2139786_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139786_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5671238
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA74300.049.4520841
GTATCAA142300.039.5140081
TCAACGC164750.033.597994
ATCAACG164900.033.4084633
CAACGCA169550.032.595295
TATCAAC173150.032.1205752
AACGCAG174850.031.7294036
ACGCAGA196200.028.2544217
CGCAGAG199800.027.723478
GTGGTAT40100.026.6931061
TGGTATC37950.026.4785062
GCAGAGT233850.023.7614829
GAGTACT139650.021.23990812-13
GTACATG158950.020.8346441
TACATGG157000.020.5368632
CAGAGTA230100.019.89612210-11
ACATGGG156850.019.831593
AGAGTAC210550.019.73099910-11
GTACTTT155100.019.09655814-15
AGTACTT152600.017.77709812-13
Produced by FastQC (version 0.11.5)