##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139786_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5671238
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.887639524209707	32.0	25.0	33.0	18.0	33.0
2	30.606852154679455	32.0	30.0	33.0	25.0	33.0
3	30.350682337789387	32.0	30.0	33.0	25.0	33.0
4	31.273614508860323	33.0	31.0	33.0	28.0	33.0
5	31.644297594281884	33.0	32.0	33.0	30.0	33.0
6	35.03283797999661	37.0	34.0	38.0	29.0	38.0
7	36.45619157580761	38.0	37.0	38.0	34.0	38.0
8	36.42483863311679	38.0	37.0	38.0	34.0	38.0
9	36.85269671278123	38.0	38.0	38.0	35.0	38.0
10-11	36.97439227554901	38.0	38.0	38.0	35.0	38.0
12-13	37.04533128392778	38.0	38.0	38.0	36.0	38.0
14-15	37.10014118610434	38.0	38.0	38.0	36.0	38.0
16-17	37.068972859188776	38.0	38.0	38.0	36.0	38.0
18-19	37.121186414677005	38.0	38.0	38.0	36.0	38.0
20-21	37.160838506736596	38.0	38.0	38.0	36.0	38.0
22-23	37.177393485224975	38.0	38.0	38.0	36.0	38.0
24-25	37.18029075617805	38.0	38.0	38.0	36.0	38.0
26-27	37.15459550004337	38.0	38.0	38.0	36.0	38.0
28-29	37.13299528667672	38.0	38.0	38.0	36.0	38.0
30-31	37.13275205932886	38.0	38.0	38.0	36.0	38.0
32-33	37.12314558620926	38.0	38.0	38.0	36.0	38.0
34-35	37.10322018709073	38.0	38.0	38.0	36.0	38.0
36-37	37.08709592295603	38.0	38.0	38.0	36.0	38.0
38-39	37.064561735813015	38.0	38.0	38.0	36.0	38.0
40-41	37.03730384579377	38.0	38.0	38.0	36.0	38.0
42-43	37.007471328690286	38.0	38.0	38.0	36.0	38.0
44-45	36.95738021376839	38.0	38.0	38.0	36.0	38.0
46-47	36.928479895979734	38.0	38.0	38.0	36.0	38.0
48-49	36.893567468647355	38.0	38.0	38.0	36.0	38.0
50-51	36.85930741715278	38.0	38.0	38.0	35.0	38.0
52-53	36.83309254870308	38.0	38.0	38.0	35.0	38.0
54-55	36.80360931766951	38.0	38.0	38.0	35.0	38.0
56-57	36.80293575941019	38.0	38.0	38.0	35.0	38.0
58-59	36.78938964559059	38.0	38.0	38.0	35.0	38.0
60-61	36.7945149177535	38.0	38.0	38.0	35.0	38.0
62-63	36.7814507274293	38.0	38.0	38.0	35.0	38.0
64-65	36.77170919403871	38.0	38.0	38.0	35.0	38.0
66-67	36.757692586240815	38.0	38.0	38.0	35.0	38.0
68-69	36.76345150051375	38.0	38.0	38.0	35.0	38.0
70-71	36.741001940987104	38.0	38.0	38.0	35.0	38.0
72-73	36.723322306331475	38.0	38.0	38.0	35.0	38.0
74-75	36.733871349660426	38.0	38.0	38.0	35.0	38.0
76-77	36.70618091700404	38.0	38.0	38.0	34.5	38.0
78-79	36.67815683449807	38.0	38.0	38.0	34.0	38.0
80-81	36.66689392735108	38.0	38.0	38.0	34.0	38.0
82-83	36.604950307949636	38.0	38.0	38.0	34.0	38.0
84-85	36.598616273039156	38.0	38.0	38.0	34.0	38.0
86-87	36.58604896551847	38.0	38.0	38.0	34.0	38.0
88-89	36.55531137587006	38.0	38.0	38.0	34.0	38.0
90-91	36.529755576178786	38.0	38.0	38.0	34.0	38.0
92-93	36.53044884388431	38.0	38.0	38.0	34.0	38.0
94-95	36.471317475203705	38.0	38.0	38.0	34.0	38.0
96-97	36.434178395924945	38.0	38.0	38.0	34.0	38.0
98-99	36.458440387436696	38.0	38.0	38.0	34.0	38.0
100	34.76843236662228	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	4.0
18	18.0
19	29.0
20	67.0
21	188.0
22	683.0
23	1835.0
24	4425.0
25	8736.0
26	14687.0
27	23404.0
28	34568.0
29	49538.0
30	67077.0
31	88354.0
32	116144.0
33	156665.0
34	227040.0
35	384107.0
36	974132.0
37	3519537.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.45841489988606	19.054569742973225	14.990007472795181	25.497007884345535
2	13.772164612690494	19.544463945104887	43.961408794482416	22.721962647722204
3	17.997640021455634	24.656732798023995	30.29278616062313	27.052841019897244
4	11.985531201476645	17.5524638535713	39.8158744175434	30.646130527408655
5	13.352751593163124	36.61909616217809	35.82131109932003	14.20684114533876
6	29.861185438445066	38.0470391369781	18.658828279907286	13.432947144669546
7	26.73927632732042	32.741598924256046	22.447250494512836	18.071874253910696
8	24.041382146191008	37.49980163061399	20.626889578607	17.831926644588005
9	24.962644840509252	17.670427515121037	21.44041565527668	35.92651198909304
10-11	24.07148139436222	27.202464435454832	28.52826843098456	20.19778573919839
12-13	24.871791661714777	24.44728117564454	28.99915327129632	21.681773891344356
14-15	21.855501391406957	26.073628015611405	26.817909951936418	25.25296064104522
16-17	21.33446877031082	29.37415075861743	27.470606946843002	21.82077352422875
18-19	21.266406029865085	28.282475537087315	29.6114269935418	20.8396914395058
20-21	22.05240288248034	27.28687221259505	29.704667874239078	20.956057030685535
22-23	21.745815942005986	27.195504088394674	29.589970251792913	21.468709717806426
24-25	21.556243081607448	27.3170189326546	29.649613892634836	21.477124093103114
26-27	21.482681415468782	27.516756862575647	29.5623595143971	21.43820220755847
28-29	21.422697165323996	27.564336412882028	29.619816977008874	21.393149444785102
30-31	21.74171272766039	27.37744798678922	29.552325265334623	21.32851402021577
32-33	21.428670509097834	27.520421474675427	29.54137770020467	21.509530316022072
34-35	21.616565389230917	27.560756948578362	29.49466646407935	21.328011198111373
36-37	21.59605381379127	27.492536235747174	29.48297094407603	21.428439006385524
38-39	21.443369517051927	27.634198070223437	29.511729474076994	21.410702938647646
40-41	21.720756153011024	27.48852499590035	29.384004147400088	21.406714703688543
42-43	21.602252595132402	27.517515319308504	29.287931146753248	21.59230093880585
44-45	21.711482410449047	27.633076611122302	29.142827207917893	21.512613770510754
46-47	21.71301352600185	27.66256678715723	28.8671632492518	21.757256437589128
48-49	21.60914791700548	27.791884595260736	28.87342421713417	21.725543270599612
50-51	21.665888881440484	27.90777186079867	28.72209825950451	21.704240998256335
52-53	21.825136660599448	27.874113715545935	28.580928114191146	21.719821509663472
54-55	21.761715209741173	27.81053654597111	28.65421529262368	21.773532951664038
56-57	21.764244160197205	27.89937633997328	28.608397367358045	21.727982132471464
58-59	21.716468869579913	27.937658530246324	28.647547743319556	21.698324856854207
60-61	21.81503105577092	27.885488174583955	28.693155580883918	21.606325188761204
62-63	21.774834492756504	27.849110811713395	28.678509773773015	21.697544921757085
64-65	21.72042820547972	27.86022621588587	28.6809206990785	21.73842487955591
66-67	21.792459999196524	27.906454000225555	28.49726116046255	21.80382484011537
68-69	21.746410239731496	27.8914662110179	28.587587966058837	21.774535583191774
70-71	21.766772666226	27.69174117395937	28.62973801618277	21.91174814363186
72-73	21.821953721916827	27.759938706204256	28.534507563403295	21.88360000847562
74-75	21.71358904002492	27.82047799512807	28.517811834242586	21.948121130604427
76-77	21.8190381362092	27.81929673037118	28.427264263643075	21.934400869776542
78-79	21.780982193236255	27.87358416967726	28.362985619218556	21.982448017867927
80-81	21.825634208485653	27.895443214646892	28.332391458497014	21.946531118370437
82-83	21.88892627926825	27.88353856465345	28.39145867396009	21.83607648211821
84-85	21.95351375296381	27.851724132565014	28.360947153782035	21.833814960689143
86-87	21.78390457010329	27.900812049195583	28.428539461659273	21.886743919041855
88-89	21.89158226543434	27.91376813389758	28.320728724747507	21.873920875920565
90-91	21.910090664652486	27.95082991800554	28.32966933598581	21.809410081356166
92-93	21.904424205709823	27.888702919258012	28.39045592546586	21.816416949566303
94-95	21.778079277003027	28.014105915102654	28.35199193972482	21.8558228681695
96-97	21.942663999930513	28.141978962015724	28.35948779051249	21.555869247541278
98-99	22.920065523757106	29.97068986172874	28.65173363952207	18.457510974992083
100	21.797644802949247	31.001160574756533	22.663475190552134	24.537719431742083
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1061.0
1	950.0
2	1178.0
3	1634.0
4	1976.5
5	2727.5
6	3599.0
7	4902.0
8	6688.0
9	7834.0
10	7945.5
11	7952.5
12	8738.5
13	10681.5
14	14512.0
15	19033.0
16	22629.0
17	24915.0
18	25152.0
19	23401.5
20	21010.5
21	19090.5
22	18734.0
23	20640.5
24	25214.0
25	32561.0
26	42595.0
27	54103.5
28	66158.0
29	79766.5
30	93923.0
31	107893.5
32	122617.0
33	137181.0
34	150917.0
35	163806.5
36	178163.5
37	191511.0
38	201311.5
39	209328.5
40	217949.5
41	225014.0
42	228427.0
43	232471.0
44	234950.5
45	235052.5
46	234161.0
47	231942.5
48	227608.5
49	221624.0
50	215150.0
51	206066.0
52	195457.0
53	184299.0
54	171868.0
55	157737.0
56	142485.0
57	127260.0
58	112448.5
59	96803.5
60	79584.0
61	63354.0
62	48965.5
63	37223.5
64	28353.5
65	21195.5
66	15516.0
67	11693.0
68	8922.5
69	6513.0
70	4728.0
71	3427.0
72	2356.0
73	1552.0
74	1021.5
75	662.0
76	409.0
77	240.0
78	142.5
79	91.5
80	61.5
81	40.0
82	28.5
83	18.0
84	10.0
85	8.0
86	7.0
87	2.5
88	0.0
89	1.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0036147310340352494
3	0.0
4	0.0
5	0.005060623447649349
6	5.2898502937101214E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.7633788878757297E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	4.0268713122667556E-4
50-51	0.00158378382161143
52-53	0.00394464825127076
54-55	0.005885242976726099
56-57	0.0067834437126424564
58-59	0.008166024904490041
60-61	0.007925031292009783
62-63	0.005626127314962738
64-65	0.003355036606910164
66-67	0.001026116504299864
68-69	4.290949383278424E-4
70-71	2.3585976485960742E-4
72-73	5.643781725850629E-4
74-75	0.0
76-77	2.2102016276527567E-4
78-79	2.531607371190045E-4
80-81	1.0210014901516749E-4
82-83	3.088222487077333E-5
84-85	6.224582307185004E-5
86-87	1.3593388092380247E-4
88-89	3.1632329421609184E-5
90-91	1.0638268447342592E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	47.0
20-21	177.0
22-23	354.0
24-25	428.0
26-27	1000.0
28-29	2148.0
30-31	6070.0
32-33	8276.0
34-35	11653.0
36-37	19170.0
38-39	24040.0
40-41	24162.0
42-43	29284.0
44-45	35862.0
46-47	36517.0
48-49	34163.0
50-51	30931.0
52-53	29772.0
54-55	32229.0
56-57	33874.0
58-59	34463.0
60-61	33895.0
62-63	33035.0
64-65	35317.0
66-67	37600.0
68-69	39155.0
70-71	38726.0
72-73	35998.0
74-75	36492.0
76-77	38793.0
78-79	40162.0
80-81	40668.0
82-83	37880.0
84-85	37547.0
86-87	39104.0
88-89	41428.0
90-91	42745.0
92-93	42641.0
94-95	52844.0
96-97	175922.0
98-99	408125.0
100-101	3988541.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.0737491962432
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.64527099149787	78.92823592139186
2	6.832297791850044	11.898275687231937
3	1.5140687749101822	3.9550693431717714
4	0.4934507649759197	1.7186643260083032
5	0.20820792230207552	0.9064722203600906
6	0.11338060761325544	0.592348475462056
7	0.05554189483267828	0.3385368714381321
8	0.036291290505438875	0.2528014981982869
9	0.026218968340696706	0.2054685486133859
>10	0.07300830978982141	0.9561978517874563
>50	0.0015705612345098747	0.10050045816205927
>100	6.331996430939134E-4	0.10348518051652945
>500	2.9461252810753002E-5	0.016648808281246538
>1k	2.9461251603909753E-5	0.027294809376888964
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.763283431236707E-5	3.526566862473414E-5
2	0.0	1.763283431236707E-5	0.0	1.763283431236707E-5	3.526566862473414E-5
3	0.0	1.763283431236707E-5	0.0	1.763283431236707E-5	3.526566862473414E-5
4	0.0	1.0579700587420243E-4	0.0	1.763283431236707E-5	3.526566862473414E-5
5	0.0	1.0579700587420243E-4	0.0	1.763283431236707E-5	3.526566862473414E-5
6	0.0	1.0579700587420243E-4	0.0	1.763283431236707E-5	3.526566862473414E-5
7	0.0	1.0579700587420243E-4	0.0	1.763283431236707E-5	3.526566862473414E-5
8	0.0	1.0579700587420243E-4	0.0	1.763283431236707E-5	3.526566862473414E-5
9	0.0	1.4106267449893656E-4	0.0	1.0579700587420243E-4	3.526566862473414E-5
10-11	1.763283431236707E-5	1.4106267449893656E-4	0.0	1.0579700587420243E-4	3.526566862473414E-5
12-13	1.763283431236707E-5	1.4106267449893656E-4	0.0	1.4106267449893656E-4	3.526566862473414E-5
14-15	3.526566862473414E-5	1.9396117743603777E-4	0.0	1.6751192596748716E-4	6.171492009328475E-5
16-17	5.2898502937101214E-5	1.9396117743603777E-4	0.0	2.2041042890458837E-4	7.053133724946828E-5
18-19	5.2898502937101214E-5	2.027775945922213E-4	0.0	2.644925146855061E-4	7.053133724946828E-5
20-21	5.2898502937101214E-5	2.1159401174840486E-4	0.0	2.9094176615405667E-4	7.053133724946828E-5
22-23	9.69805887180189E-5	2.2922684606077192E-4	0.0	3.3502385193497433E-4	7.934775440565181E-5
24-25	1.5869550881130362E-4	2.2922684606077192E-4	0.0	3.6147310340352496E-4	8.816417156183535E-5
26-27	1.5869550881130362E-4	2.2922684606077192E-4	0.0	3.702895205597085E-4	8.816417156183535E-5
28-29	1.5869550881130362E-4	2.2922684606077192E-4	0.0	4.320044406529932E-4	8.816417156183535E-5
30-31	1.9396117743603777E-4	2.5567609752932255E-4	0.0	8.640088813059865E-4	8.816417156183535E-5
32-33	2.1159401174840486E-4	2.6449251468550603E-4	0.0	0.0018602640199547257	8.816417156183535E-5
34-35	2.1159401174840486E-4	3.6147310340352496E-4	0.0	0.0030240310845709526	1.0579700587420243E-4
36-37	2.1159401174840486E-4	4.5845369212154383E-4	0.0	0.0048754786873694955	1.0579700587420243E-4
38-39	2.1159401174840486E-4	4.5845369212154383E-4	0.0	0.007635017257254941	1.0579700587420243E-4
40-41	2.1159401174840486E-4	5.113521950586451E-4	0.0	0.010949990107979951	1.0579700587420243E-4
42-43	2.1159401174840486E-4	5.289850293710122E-4	0.0	0.01423851370723641	1.1461342303038596E-4
44-45	2.1159401174840486E-4	5.818835323081133E-4	0.0	0.01777389698686601	1.234298401865695E-4
46-47	2.1159401174840486E-4	6.171492009328475E-4	0.0	0.021732468289992413	1.234298401865695E-4
48-49	2.1159401174840486E-4	6.171492009328475E-4	0.0	0.025999614193585246	1.234298401865695E-4
50-51	2.1159401174840486E-4	6.788641210261322E-4	0.0	0.030531252611863582	1.234298401865695E-4
52-53	2.2922684606077192E-4	6.876805381823157E-4	0.0	0.03461325375517656	1.3224625734275304E-4
54-55	2.46859680373139E-4	6.876805381823157E-4	0.0	0.03840431313233548	1.4106267449893656E-4
56-57	2.46859680373139E-4	6.876805381823157E-4	0.0	0.041983778497746	1.4987909165512008E-4
58-59	2.821253489978731E-4	6.876805381823157E-4	0.0	0.04581010354352965	1.5869550881130362E-4
60-61	2.821253489978731E-4	7.141297896508664E-4	0.0	0.0496364285893133	1.5869550881130362E-4
62-63	2.821253489978731E-4	7.40579041119417E-4	0.0	0.05322471037188001	1.5869550881130362E-4
64-65	2.821253489978731E-4	9.169073842430877E-4	0.0	0.05654849963976119	1.6751192596748716E-4
66-67	2.821253489978731E-4	9.169073842430877E-4	0.0	0.06031310976545157	1.763283431236707E-4
68-69	2.821253489978731E-4	9.433566357116383E-4	0.0	0.06411298555976666	1.763283431236707E-4
70-71	2.821253489978731E-4	9.786223043363725E-4	0.0	0.06781588076536375	1.763283431236707E-4
72-73	2.821253489978731E-4	9.962551386487396E-4	0.0	0.07063713425534249	1.9396117743603777E-4
74-75	2.821253489978731E-4	0.0010315208072734735	0.0	0.07267372661842089	1.9396117743603777E-4
76-77	2.821253489978731E-4	0.0010756028930543912	0.0	0.07465742047856218	2.027775945922213E-4
78-79	2.821253489978731E-4	0.0011108685616791254	0.0	0.07605923080639536	2.1159401174840486E-4
80-81	2.821253489978731E-4	0.0011108685616791254	0.0	0.07642952032695507	2.1159401174840486E-4
82-83	2.821253489978731E-4	0.0011108685616791254	0.0	0.07698495460779464	2.1159401174840486E-4
84-85	2.821253489978731E-4	0.0011108685616791254	0.0	0.07717009936807448	2.2041042890458837E-4
86-87	2.821253489978731E-4	0.0011285013959914925	0.0	0.07728471279110487	2.3804326321695546E-4
88	2.821253489978731E-4	0.001216665567553328	0.0	0.07728471279110487	2.6449251468550603E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7430	0.0	49.452084	1
GTATCAA	14230	0.0	39.514008	1
TCAACGC	16475	0.0	33.59799	4
ATCAACG	16490	0.0	33.408463	3
CAACGCA	16955	0.0	32.59529	5
TATCAAC	17315	0.0	32.120575	2
AACGCAG	17485	0.0	31.729403	6
ACGCAGA	19620	0.0	28.254421	7
CGCAGAG	19980	0.0	27.72347	8
GTGGTAT	4010	0.0	26.693106	1
TGGTATC	3795	0.0	26.478506	2
GCAGAGT	23385	0.0	23.761482	9
GAGTACT	13965	0.0	21.239908	12-13
GTACATG	15895	0.0	20.834644	1
TACATGG	15700	0.0	20.536863	2
CAGAGTA	23010	0.0	19.896122	10-11
ACATGGG	15685	0.0	19.83159	3
AGAGTAC	21055	0.0	19.730999	10-11
GTACTTT	15510	0.0	19.096558	14-15
AGTACTT	15260	0.0	17.777098	12-13
>>END_MODULE