FastQCFastQC Report
Fri 27 May 2016
SRR2139785_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139785_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8263713
Sequences flagged as poor quality0
Sequence length19-100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103650.047.7334481
GTATCAA185200.032.508031
ATCAACG235800.029.2154123
TCAACGC236650.029.1104764
CAACGCA241850.028.5204945
AACGCAG248800.027.7931296
TATCAAC252000.027.5623552
TGGTATC52650.025.5759472
GTGGTAT50350.024.5011921
ACGCAGA288100.023.9264587
CGCAGAG292550.023.5773588
GCAGAGT342800.020.1338989
GAGTACT205350.019.87223812-13
CAGAGTA334450.018.75992410-11
AGAGTAC310050.018.60420410-11
GTACTTT225200.018.03410114-15
TACATGG231200.017.585572
ACATGGG230550.017.2388633
AGTACTT229150.016.55723412-13
TTATCAA67600.016.192811
Produced by FastQC (version 0.11.5)