##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139785_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8263713
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	22.916379961404758	18.0	18.0	30.0	18.0	33.0
2	30.479942611753337	32.0	28.0	33.0	27.0	33.0
3	32.04650476123747	33.0	32.0	33.0	30.0	33.0
4	32.38717499022534	33.0	33.0	33.0	31.0	34.0
5	32.8423197901476	33.0	33.0	34.0	32.0	34.0
6	36.708845285406206	38.0	37.0	38.0	34.0	38.0
7	36.98058197326069	38.0	38.0	38.0	35.0	38.0
8	37.034230859663204	38.0	38.0	38.0	36.0	38.0
9	37.17222161515048	38.0	38.0	38.0	36.0	38.0
10-11	37.20476164891012	38.0	38.0	38.0	36.0	38.0
12-13	37.22228518826827	38.0	38.0	38.0	36.0	38.0
14-15	37.212354422279674	38.0	38.0	38.0	36.0	38.0
16-17	37.16556788697768	38.0	38.0	38.0	36.0	38.0
18-19	37.18788920912428	38.0	38.0	38.0	36.0	38.0
20-21	37.20695309369693	38.0	38.0	38.0	36.0	38.0
22-23	37.21351787311078	38.0	38.0	38.0	36.0	38.0
24-25	37.21707572540852	38.0	38.0	38.0	36.0	38.0
26-27	37.18647707142031	38.0	38.0	38.0	36.0	38.0
28-29	37.15937411616952	38.0	38.0	38.0	36.0	38.0
30-31	37.160352022191574	38.0	38.0	38.0	36.0	38.0
32-33	37.15265944917316	38.0	38.0	38.0	36.0	38.0
34-35	37.13142945212664	38.0	38.0	38.0	36.0	38.0
36-37	37.11227457883929	38.0	38.0	38.0	36.0	38.0
38-39	37.09343270858979	38.0	38.0	38.0	36.0	38.0
40-41	37.07067473457966	38.0	38.0	38.0	36.0	38.0
42-43	37.044265550226015	38.0	38.0	38.0	36.0	38.0
44-45	36.99220036232728	38.0	38.0	38.0	36.0	38.0
46-47	36.963275312536794	38.0	38.0	38.0	36.0	38.0
48-49	36.92991965226662	38.0	38.0	38.0	36.0	38.0
50-51	36.8878595920484	38.0	38.0	38.0	35.5	38.0
52-53	36.85847642509218	38.0	38.0	38.0	35.0	38.0
54-55	36.83113065318804	38.0	38.0	38.0	35.0	38.0
56-57	36.829074386234346	38.0	38.0	38.0	35.0	38.0
58-59	36.81535804032952	38.0	38.0	38.0	35.0	38.0
60-61	36.82200079038029	38.0	38.0	38.0	35.0	38.0
62-63	36.80862220243773	38.0	38.0	38.0	35.0	38.0
64-65	36.796823723106186	38.0	38.0	38.0	35.0	38.0
66-67	36.784969655845984	38.0	38.0	38.0	35.0	38.0
68-69	36.79032088388995	38.0	38.0	38.0	35.0	38.0
70-71	36.76751935504625	38.0	38.0	38.0	35.0	38.0
72-73	36.75013419298823	38.0	38.0	38.0	35.0	38.0
74-75	36.759718442743385	38.0	38.0	38.0	35.0	38.0
76-77	36.738215938725915	38.0	38.0	38.0	35.0	38.0
78-79	36.71034055113677	38.0	38.0	38.0	34.5	38.0
80-81	36.69932741282264	38.0	38.0	38.0	34.5	38.0
82-83	36.63814603631417	38.0	38.0	38.0	34.0	38.0
84-85	36.635213384565816	38.0	38.0	38.0	34.0	38.0
86-87	36.61834094201373	38.0	38.0	38.0	34.0	38.0
88-89	36.590340238545224	38.0	38.0	38.0	34.0	38.0
90-91	36.56657376081587	38.0	38.0	38.0	34.0	38.0
92-93	36.567715455175694	38.0	38.0	38.0	34.0	38.0
94-95	36.50896889762518	38.0	38.0	38.0	34.0	38.0
96-97	36.47065890920793	38.0	38.0	38.0	34.0	38.0
98-99	36.4956259593659	38.0	38.0	38.0	34.0	38.0
100	34.818868373998875	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	17.0
19	37.0
20	109.0
21	300.0
22	907.0
23	2534.0
24	5990.0
25	11349.0
26	19651.0
27	31575.0
28	47078.0
29	67659.0
30	92397.0
31	121607.0
32	157753.0
33	210246.0
34	304817.0
35	517259.0
36	1403095.0
37	5269331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.4000419666075	18.31559251876245	22.36192133003651	22.922444184593534
2	14.186754022816025	20.84529659986277	42.22341891864092	22.74453045868029
3	18.666584863244886	25.057102055698206	30.54751538442828	25.728797696628625
4	12.55974160767684	18.230570204942982	38.55725628419089	30.65243190318928
5	13.342662704507577	37.017862147649645	36.08557043812386	13.553904709718923
6	30.146954629441723	37.98222758865967	18.427057009644702	13.443760772253905
7	26.741199748829615	32.557919182333656	22.66845424084791	18.03242682798882
8	24.086908632959542	37.57780552156155	20.63801102482625	17.697274820652652
9	24.9318798946672	17.59902600683252	21.45472622294603	36.01436787555424
10-11	24.267293648750872	27.162941162162817	28.49269450669451	20.077070682391803
12-13	24.986897536252773	24.41016526106364	28.947586877714652	21.655350324968932
14-15	21.929978691176714	26.078210847835592	26.646423950105717	25.345386510881973
16-17	21.47032453813437	29.287651930796727	27.396825131753726	21.845198399315173
18-19	21.4871148114655	28.20944410823561	29.347830690635067	20.95561038966382
20-21	22.21820594590951	27.253761285793583	29.45370520851876	21.074327559778144
22-23	21.95976639645837	27.166071879250904	29.31874871306212	21.5554130112286
24-25	21.730396733812626	27.294200726263167	29.376098668194793	21.59930387172941
26-27	21.661466824027617	27.468482926090665	29.290245384078084	21.579804865803634
28-29	21.62259894830193	27.458540805099307	29.359140671168905	21.559719575429856
30-31	21.882802223406923	27.326168688347636	29.229737068213126	21.561292020032322
32-33	21.641471446545992	27.488076634631454	29.21875459626137	21.651697322561187
34-35	21.825779898409788	27.498461076632612	29.19325170709594	21.48250731786166
36-37	21.797643631073296	27.412558850107953	29.169615317202478	21.62018220161627
38-39	21.631835687217947	27.560254653276477	29.264492698400574	21.543416961105002
40-41	21.916928434490522	27.46122907228606	29.062167395138527	21.55967509808489
42-43	21.818182043012268	27.472838177091795	28.957764048581275	21.75121573131466
44-45	21.871749486636627	27.58508817507408	28.826137287749255	21.71702505054004
46-47	21.908873715495893	27.62550950479308	28.584281746978846	21.88133503273218
48-49	21.834895084061674	27.72193523181793	28.568884543908645	21.874285140211754
50-51	21.841169153897038	27.88449042234959	28.453630878675728	21.820709545077644
52-53	21.99558401218394	27.785102396508314	28.35825674116817	21.86105685013958
54-55	21.968475396468833	27.692757898367482	28.39297670830608	21.94578999685761
56-57	21.920271338932025	27.840605336764728	28.37633246450208	21.862790859801162
58-59	21.91459766900861	27.82784088353712	28.38995904698028	21.86760240047399
60-61	21.959289415831	27.770135069175595	28.483552269694094	21.78702324529931
62-63	21.95542782298947	27.795423397613856	28.426898590891938	21.822250188504743
64-65	21.944144326872888	27.7845853226936	28.362468028495062	21.90880232193845
66-67	21.96310214339605	27.792918435801827	28.281929808636413	21.962049612165703
68-69	21.91037850432273	27.759346386582795	28.36705637127197	21.963218737822505
70-71	21.94018115038471	27.618080270763063	28.396934147549818	22.04480443130241
72-73	21.98741583241139	27.637068025990015	28.317789297099967	22.057726844498625
74-75	21.840498402629287	27.80769127271739	28.248146800246793	22.103663524406535
76-77	22.005273130390275	27.724530876424723	28.20803362488603	22.062162368298974
78-79	21.98722995921609	27.755272353282763	28.11933232170808	22.138165365793068
80-81	22.014222958492763	27.777857334569884	28.107988089544776	22.099931617392578
82-83	22.051728358555543	27.74947480343378	28.19148069112608	22.007316146884595
84-85	22.119390519145057	27.734778006483097	28.151795623455683	21.994035850916166
86-87	21.980708504029376	27.801542537733354	28.177217355297245	22.04053160294002
88-89	22.058890799434185	27.813085723534975	28.08329560462503	22.044727872405808
90-91	22.068907043513146	27.830280881410935	28.104292037473737	21.99652003760218
92-93	22.117527450608172	27.747237688504732	28.13572893225472	21.999505928632377
94-95	22.00735067767076	27.856551078808778	28.10086729731226	22.035230946208202
96-97	22.131341848228892	27.95166560484259	28.13048208445427	21.786510462474247
98-99	23.1196744643277	29.841895911139883	28.391228521222118	18.6472011033103
100	22.04816642664257	30.78634758306854	22.36604262730155	24.799443362987343
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1361.0
1	1471.5
2	1950.0
3	2424.5
4	2921.5
5	3828.0
6	4792.0
7	7147.0
8	9390.0
9	10071.0
10	10328.0
11	10561.0
12	11814.0
13	14608.0
14	20573.0
15	27973.0
16	32508.5
17	33561.0
18	32443.0
19	30027.5
20	27163.0
21	25144.0
22	25347.0
23	28671.5
24	35414.5
25	46080.0
26	60295.5
27	76675.5
28	94434.5
29	114664.5
30	135508.5
31	156295.0
32	178251.0
33	200116.5
34	221477.5
35	241090.5
36	261380.0
37	280735.0
38	296248.0
39	308826.5
40	320512.5
41	328870.5
42	333423.5
43	339584.5
44	343782.0
45	345755.0
46	345667.0
47	342404.5
48	337671.0
49	328901.5
50	317867.5
51	304314.5
52	289124.5
53	273406.5
54	255352.0
55	235591.0
56	214576.0
57	193628.5
58	171778.5
59	147741.0
60	121815.5
61	97559.5
62	75925.0
63	57416.0
64	43239.5
65	32044.5
66	23711.0
67	18160.5
68	13759.5
69	9991.0
70	7177.0
71	5014.0
72	3484.0
73	2403.5
74	1567.0
75	962.5
76	569.5
77	329.0
78	207.5
79	124.0
80	66.5
81	40.5
82	29.5
83	20.5
84	15.5
85	11.5
86	8.5
87	6.0
88	4.0
89	3.0
90	2.0
91	1.5
92	0.5
93	0.0
94	0.5
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.002698544830876871
3	0.0
4	0.0
5	0.002480725068743312
6	7.260658737785304E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.2101677080664032E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.2130337811106015E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	6.151161597658425E-6
42-43	0.0
44-45	0.0
46-47	6.265911499012805E-6
48-49	5.111467862119101E-4
50-51	0.0017149952323132541
52-53	0.004217575519044558
54-55	0.006256097766412355
56-57	0.006951547648166509
58-59	0.008453219252457773
60-61	0.00820435324295768
62-63	0.006132760924690819
64-65	0.0037263764369506423
66-67	0.001441343648705804
68-69	6.216459414189245E-4
70-71	2.79285274922294E-4
72-73	6.728281245451545E-4
74-75	1.3835138415371669E-5
76-77	2.788680356019666E-4
78-79	2.600873471723233E-4
80-81	4.253529649795561E-5
82-83	0.0
84-85	1.6577464292322105E-4
86-87	1.6714394741971166E-4
88-89	3.665352442330628E-5
90-91	1.4797014554343515E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	73.0
20-21	231.0
22-23	448.0
24-25	614.0
26-27	1450.0
28-29	3208.0
30-31	10725.0
32-33	14312.0
34-35	20600.0
36-37	33503.0
38-39	40035.0
40-41	40653.0
42-43	47027.0
44-45	56569.0
46-47	57348.0
48-49	53003.0
50-51	48011.0
52-53	47099.0
54-55	50765.0
56-57	53160.0
58-59	53530.0
60-61	52715.0
62-63	51218.0
64-65	54887.0
66-67	57971.0
68-69	59667.0
70-71	58371.0
72-73	54948.0
74-75	55258.0
76-77	58371.0
78-79	59865.0
80-81	60067.0
82-83	56894.0
84-85	56363.0
86-87	58797.0
88-89	61925.0
90-91	63171.0
92-93	62817.0
94-95	77503.0
96-97	256379.0
98-99	588348.0
100-101	5725814.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.25504484410614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.15275540352093	77.76129960150429
2	7.0647432521319695	12.187394920494784
3	1.5972236625152378	4.133057959089579
4	0.5715987615111273	1.9721310722791126
5	0.25656134844532674	1.1064855307708
6	0.12024487540744244	0.6223036272325743
7	0.07583117871184235	0.45785752042599726
8	0.04622385543249942	0.31896325785581736
9	0.02658412353834882	0.20637132911473818
>10	0.08708856234710761	1.1164105498851988
>50	8.536055872375351E-4	0.05168254354592777
>100	2.4905742637108504E-4	0.03646090615572099
>500	4.2313424560326334E-5	0.02958118164537618
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.2101097896308838E-5
3	0.0	0.0	0.0	1.2101097896308838E-5	1.2101097896308838E-5
4	0.0	2.4202195792617677E-5	0.0	1.2101097896308838E-5	2.4202195792617677E-5
5	0.0	2.4202195792617677E-5	0.0	1.2101097896308838E-5	2.4202195792617677E-5
6	4.8404391585235354E-5	2.4202195792617677E-5	0.0	1.2101097896308838E-5	2.4202195792617677E-5
7	4.8404391585235354E-5	2.4202195792617677E-5	0.0	1.2101097896308838E-5	2.4202195792617677E-5
8	4.8404391585235354E-5	3.630329368892651E-5	0.0	1.2101097896308838E-5	2.4202195792617677E-5
9	4.8404391585235354E-5	7.260658737785302E-5	0.0	2.4202195792617677E-5	2.4202195792617677E-5
10-11	4.8404391585235354E-5	7.260658737785302E-5	0.0	5.445494053338977E-5	2.4202195792617677E-5
12-13	4.8404391585235354E-5	7.865713632600744E-5	0.0	6.050548948154419E-5	2.4202195792617677E-5
14-15	4.8404391585235354E-5	1.0890988106677955E-4	0.0	6.050548948154419E-5	3.630329368892651E-5
16-17	8.470768527416187E-5	1.1496043001493396E-4	0.0	9.680878317047071E-5	3.630329368892651E-5
18-19	8.470768527416187E-5	1.3311207685939722E-4	0.0	9.680878317047071E-5	3.630329368892651E-5
20-21	8.470768527416187E-5	1.4521317475570605E-4	0.0	9.680878317047071E-5	3.630329368892651E-5
22-23	8.470768527416187E-5	1.6336482160016932E-4	0.0	1.1496043001493396E-4	4.8404391585235354E-5
24-25	8.470768527416187E-5	1.87567017392787E-4	0.0	1.3311207685939722E-4	6.050548948154419E-5
26-27	8.470768527416187E-5	1.9361756634094141E-4	0.0	1.6336482160016932E-4	6.050548948154419E-5
28-29	9.680878317047071E-5	1.9361756634094141E-4	0.0	2.6622415371879444E-4	6.050548948154419E-5
30-31	9.680878317047071E-5	2.4202195792617676E-4	0.0	6.413581885043684E-4	6.050548948154419E-5
32-33	9.680878317047071E-5	2.480725068743312E-4	0.0	0.0018756701739278698	7.260658737785302E-5
34-35	1.0890988106677955E-4	3.2672964320033863E-4	0.0	0.003291498627796004	7.260658737785302E-5
36-37	1.0890988106677955E-4	3.9933623057819166E-4	0.0	0.0054152413085982055	8.470768527416187E-5
38-39	1.0890988106677955E-4	4.114373284745005E-4	0.0	0.008785397072720217	8.470768527416187E-5
40-41	1.0890988106677955E-4	4.2353842637080937E-4	0.0	0.012560939616368574	8.470768527416187E-5
42-43	1.0890988106677955E-4	4.2353842637080937E-4	0.0	0.01625177447474277	8.470768527416187E-5
44-45	1.0890988106677955E-4	4.598417200597359E-4	0.0	0.0208804444200809	9.07582342223163E-5
46-47	1.270615279112428E-4	4.961450137486624E-4	0.0	0.02578138906808598	9.680878317047071E-5
48-49	1.4521317475570605E-4	4.961450137486624E-4	0.0	0.03097276006560247	1.2101097896308838E-4
50-51	1.573142726520149E-4	5.808526990228242E-4	0.0	0.03633959698261544	1.2101097896308838E-4
52-53	1.573142726520149E-4	6.05054894815442E-4	0.0	0.041488614137494856	1.2101097896308838E-4
54-55	1.6336482160016932E-4	6.05054894815442E-4	0.0	0.04612938518072929	1.2101097896308838E-4
56-57	1.6941537054832373E-4	6.05054894815442E-4	0.0	0.050358718895489235	1.270615279112428E-4
58-59	1.6941537054832373E-4	6.05054894815442E-4	0.0	0.05464855809973071	1.3311207685939722E-4
60-61	1.6941537054832373E-4	6.353076395562141E-4	0.0	0.05890814455923142	1.3311207685939722E-4
62-63	1.6941537054832373E-4	6.595098353488317E-4	0.0	0.06335529803612493	1.3311207685939722E-4
64-65	1.6941537054832373E-4	8.22874656949001E-4	0.0	0.0673002559503216	1.3311207685939722E-4
66-67	1.6941537054832373E-4	8.349757548453098E-4	0.0	0.07159009515456309	1.3311207685939722E-4
68-69	1.6941537054832373E-4	8.531274016897731E-4	0.0	0.07586178271196012	1.3311207685939722E-4
70-71	1.7546591949647817E-4	8.894306953786996E-4	0.0	0.07952236482559354	1.4521317475570605E-4
72-73	1.9361756634094141E-4	9.136328911713172E-4	0.0	0.08269285247442645	1.6941537054832373E-4
74-75	2.0571866423725024E-4	9.559867338083982E-4	0.0	0.08511307205368822	1.6941537054832373E-4
76-77	2.0571866423725024E-4	9.741383806528615E-4	0.0	0.08720656198974963	1.6941537054832373E-4
78-79	2.0571866423725024E-4	9.801889296010158E-4	0.0	0.08861028934572146	1.6941537054832373E-4
80-81	2.0571866423725024E-4	9.801889296010158E-4	0.0	0.08913668710421091	1.87567017392787E-4
82-83	2.0571866423725024E-4	9.801889296010158E-4	0.0	0.08945131564951493	2.0571866423725024E-4
84-85	2.2992086002986793E-4	9.922900274973248E-4	0.0	0.08968123650954479	2.0571866423725024E-4
86-87	2.2992086002986793E-4	0.0010164922232899424	0.0	0.08974779254797449	2.0571866423725024E-4
88	2.2992086002986793E-4	0.0010769977127714867	0.0	0.08975384309692265	2.0571866423725024E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10365	0.0	47.733448	1
GTATCAA	18520	0.0	32.50803	1
ATCAACG	23580	0.0	29.215412	3
TCAACGC	23665	0.0	29.110476	4
CAACGCA	24185	0.0	28.520494	5
AACGCAG	24880	0.0	27.793129	6
TATCAAC	25200	0.0	27.562355	2
TGGTATC	5265	0.0	25.575947	2
GTGGTAT	5035	0.0	24.501192	1
ACGCAGA	28810	0.0	23.926458	7
CGCAGAG	29255	0.0	23.577358	8
GCAGAGT	34280	0.0	20.133898	9
GAGTACT	20535	0.0	19.872238	12-13
CAGAGTA	33445	0.0	18.759924	10-11
AGAGTAC	31005	0.0	18.604204	10-11
GTACTTT	22520	0.0	18.034101	14-15
TACATGG	23120	0.0	17.58557	2
ACATGGG	23055	0.0	17.238863	3
AGTACTT	22915	0.0	16.557234	12-13
TTATCAA	6760	0.0	16.19281	1
>>END_MODULE