FastQCFastQC Report
Fri 27 May 2016
SRR2139784_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139784_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7534869
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85800.046.680951
GTATCAA153200.037.71931
TCAACGC172050.033.258154
ATCAACG173050.033.040973
CAACGCA177350.032.2642485
AACGCAG184150.031.1179626
TATCAAC189350.030.4952322
ACGCAGA211350.027.1131867
CGCAGAG214100.026.7649338
GCAGAGT256050.022.3292269
TACATGG193950.021.8104692
GTACATG196550.021.7200151
ACATGGG196000.020.8750723
GAGTACT148300.020.3992212-13
GTGGTAT48050.020.1637171
TGGTATC46350.019.6901322
CAGAGTA252850.019.11399810-11
AGAGTAC229600.018.3182810-11
GTACTTT167300.018.16042514-15
CATGGGG143750.017.9922834
Produced by FastQC (version 0.11.5)