##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139784_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7534869
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.20899328176774	31.0	18.0	33.0	18.0	33.0
2	29.062537782674124	31.0	27.0	33.0	18.0	33.0
3	30.991168260523175	32.0	31.0	33.0	27.0	33.0
4	31.688810117335816	33.0	31.0	33.0	29.0	33.0
5	32.523739297922766	33.0	33.0	33.0	32.0	34.0
6	36.37258378347387	38.0	37.0	38.0	34.0	38.0
7	36.56173265918757	38.0	37.0	38.0	34.0	38.0
8	36.78972242251325	38.0	38.0	38.0	35.0	38.0
9	37.022266345970976	38.0	38.0	38.0	36.0	38.0
10-11	37.120684978066635	38.0	38.0	38.0	36.0	38.0
12-13	37.18163580547983	38.0	38.0	38.0	36.0	38.0
14-15	37.1715405403863	38.0	38.0	38.0	36.0	38.0
16-17	37.12546522043051	38.0	38.0	38.0	36.0	38.0
18-19	37.15397341347275	38.0	38.0	38.0	36.0	38.0
20-21	37.179107021064475	38.0	38.0	38.0	36.0	38.0
22-23	37.18821011277265	38.0	38.0	38.0	36.0	38.0
24-25	37.19025205853135	38.0	38.0	38.0	36.0	38.0
26-27	37.16105364213725	38.0	38.0	38.0	36.0	38.0
28-29	37.1351152273747	38.0	38.0	38.0	36.0	38.0
30-31	37.13346614537441	38.0	38.0	38.0	36.0	38.0
32-33	37.12567307836778	38.0	38.0	38.0	36.0	38.0
34-35	37.107285092497804	38.0	38.0	38.0	36.0	38.0
36-37	37.09280619553026	38.0	38.0	38.0	36.0	38.0
38-39	37.07501165503999	38.0	38.0	38.0	36.0	38.0
40-41	37.05010765869524	38.0	38.0	38.0	36.0	38.0
42-43	37.02852728727028	38.0	38.0	38.0	36.0	38.0
44-45	36.982682412211965	38.0	38.0	38.0	36.0	38.0
46-47	36.963226413381335	38.0	38.0	38.0	36.0	38.0
48-49	36.93491133938065	38.0	38.0	38.0	36.0	38.0
50-51	36.89857031661926	38.0	38.0	38.0	35.5	38.0
52-53	36.86959800698743	38.0	38.0	38.0	35.0	38.0
54-55	36.836859194541276	38.0	38.0	38.0	35.0	38.0
56-57	36.83370022383323	38.0	38.0	38.0	35.0	38.0
58-59	36.8153839534339	38.0	38.0	38.0	35.0	38.0
60-61	36.81937857911683	38.0	38.0	38.0	35.0	38.0
62-63	36.80151348352854	38.0	38.0	38.0	35.0	38.0
64-65	36.78621757718189	38.0	38.0	38.0	35.0	38.0
66-67	36.77076818944983	38.0	38.0	38.0	35.0	38.0
68-69	36.775535445776015	38.0	38.0	38.0	35.0	38.0
70-71	36.74811420482075	38.0	38.0	38.0	35.0	38.0
72-73	36.725547874546294	38.0	38.0	38.0	35.0	38.0
74-75	36.73407486115022	38.0	38.0	38.0	35.0	38.0
76-77	36.70664666670021	38.0	38.0	38.0	34.5	38.0
78-79	36.678396304664076	38.0	38.0	38.0	34.0	38.0
80-81	36.66686681679151	38.0	38.0	38.0	34.0	38.0
82-83	36.604231287446865	38.0	38.0	38.0	34.0	38.0
84-85	36.5972475984742	38.0	38.0	38.0	34.0	38.0
86-87	36.57900773332243	38.0	38.0	38.0	34.0	38.0
88-89	36.5472339502773	38.0	38.0	38.0	34.0	38.0
90-91	36.52201573194019	38.0	38.0	38.0	34.0	38.0
92-93	36.521717883992245	38.0	38.0	38.0	34.0	38.0
94-95	36.464100101541014	38.0	38.0	38.0	34.0	38.0
96-97	36.42620985176798	38.0	38.0	38.0	34.0	38.0
98-99	36.448837287811244	38.0	38.0	38.0	34.0	38.0
100	34.74527836556626	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	19.0
19	35.0
20	68.0
21	269.0
22	868.0
23	2416.0
24	5594.0
25	11052.0
26	19238.0
27	30124.0
28	44937.0
29	63762.0
30	85732.0
31	113713.0
32	148683.0
33	201039.0
34	290676.0
35	491787.0
36	1298090.0
37	4726764.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.20802485086337	18.265785377290566	14.415671991112255	27.11051778073381
2	13.710427616460057	19.251161876463172	43.972304225624285	23.066106281452488
3	18.173162665469032	25.15138617539336	29.866066682778424	26.809384476359178
4	12.196204074682653	17.950929206599344	38.482765924663056	31.370100794054945
5	13.77023092905828	36.758684807948434	35.614703702204444	13.856380560788844
6	30.563205918514257	37.58556226097777	18.512278390160724	13.33895343034725
7	27.295803019269478	32.28212726724247	22.222775206841686	18.199294506646364
8	24.29511382347855	37.31498185303553	20.53794166826258	17.851962655223335
9	24.920804860708262	17.55267410753923	21.242506007735503	36.28401502401701
10-11	24.396893960598387	26.945378877854413	28.375476733570288	20.282250427976916
12-13	25.054383825385685	24.076112006725	28.841496779837843	22.02800738805147
14-15	21.994715502021336	25.78064064551089	26.47744771674199	25.747196135725787
16-17	21.760789736357726	29.085588614745657	27.114379825316142	22.039241823580475
18-19	21.649626556214844	28.149235242178726	28.96761708796795	21.233521113638474
20-21	22.439849800186153	27.201804489404648	28.98458120049695	21.373764509912256
22-23	22.120537325920672	27.144065110659028	28.83269829859887	21.90269926482143
24-25	21.910191028526725	27.216813303003505	28.986450595416173	21.886545073053593
26-27	21.89511113447382	27.40740917730948	28.863237698223777	21.834241989992933
28-29	21.788657005585222	27.4664675605464	28.88116195336434	21.86371348050404
30-31	22.0858140877019	27.23150635942748	28.849470087016822	21.8332094658538
32-33	21.826193884008152	27.410661231648998	28.829967388563173	21.933177495779677
34-35	22.007623689928334	27.436175534233726	28.79481874999917	21.76138202583877
36-37	21.938843292543016	27.394782594734966	28.768858524524692	21.897515588197322
38-39	21.809542666149888	27.487542981462127	28.899738438747235	21.80317591364075
40-41	22.13481145959808	27.341662576998605	28.704662911180655	21.818863052222657
42-43	21.9633506981284	27.42768938852855	28.61899804808381	21.989961865259243
44-45	22.072914538592446	27.4636640416283	28.504652077188652	21.958769342590607
46-47	22.067265899160805	27.513461007302304	28.300056240545402	22.119216852991492
48-49	21.95673393257899	27.635656304910267	28.341106544165896	22.066503218344845
50-51	22.01555307170612	27.723300976649256	28.252675373421322	22.008470578223303
52-53	22.1996732917829	27.638328776227837	28.13388625884571	22.028111673143556
54-55	22.084943311693277	27.576470100962503	28.208686103049303	22.129900484294918
56-57	22.097133055931682	27.71685945062358	28.15858438868537	22.027423104759364
58-59	22.0623790102008	27.7505251242454	28.190602523172792	21.996493342381008
60-61	22.145097028294344	27.668017952853997	28.229916613278718	21.95696840557294
62-63	22.08273851921398	27.719834204185272	28.2112128174368	21.986214459163946
64-65	22.077691220063517	27.692246454114784	28.17541523017968	22.054647095642018
66-67	22.12504085750031	27.697855596691532	28.05577768062278	22.121325865185376
68-69	22.09794672308056	27.69782752784873	28.1163340678124	22.087891681258316
70-71	22.114308417563247	27.49292272711167	28.152362470965276	22.24040638435981
72-73	22.138630400341018	27.55050810441188	28.113238367787314	22.197623127459785
74-75	21.995670501891006	27.64581950792885	28.07599625836007	22.28251373182008
76-77	22.13921083409044	27.620667832992712	27.98531525746757	22.254806075449274
78-79	22.092385353196608	27.614632784747467	27.99347105041073	22.29951081164519
80-81	22.121949387504706	27.677690890542827	27.957884938002493	22.242474783949977
82-83	22.16246133077994	27.684684733953468	27.98474940094462	22.168104534321973
84-85	22.298970345957173	27.58293840377649	27.98720273189114	22.130888518375194
86-87	22.109431282053883	27.675795544029853	28.034687042213267	22.180086131702993
88-89	22.209283282866863	27.64730238428666	27.965835562298512	22.177578770547964
90-91	22.1917485265871	27.691461178544802	28.004042984082123	22.112747310785974
92-93	22.17322841171376	27.681049879207535	28.039324888220353	22.10639682085835
94-95	22.12215010939699	27.770947470776896	27.993215436105036	22.11368698372108
96-97	22.231598415634778	27.90501013571983	28.0019025025232	21.861488946122183
98-99	23.267309961273238	29.767834981997254	28.294536029403734	18.670319027325775
100	22.116975262094925	30.699175040385086	22.238810727273574	24.945038970246415
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	670.0
1	663.5
2	1042.5
3	1554.5
4	2053.5
5	3091.0
6	4020.5
7	4878.5
8	6043.0
9	6848.5
10	7004.5
11	7283.5
12	8296.5
13	10324.5
14	14185.0
15	18804.5
16	22769.5
17	25703.0
18	26297.0
19	24694.5
20	22368.0
21	20324.0
22	19989.0
23	22506.5
24	27995.5
25	36483.0
26	48206.0
27	61914.0
28	76864.0
29	94043.5
30	112044.5
31	130700.0
32	150703.0
33	170435.5
34	190664.5
35	210551.0
36	231381.0
37	251393.5
38	266263.0
39	278197.0
40	291698.0
41	304063.0
42	311312.0
43	318289.5
44	324256.5
45	329194.0
46	331941.5
47	330494.0
48	326236.0
49	318917.0
50	310860.5
51	298790.0
52	281275.0
53	263969.0
54	246728.0
55	226139.0
56	205130.0
57	184230.5
58	161437.0
59	138213.5
60	114591.5
61	92460.0
62	72816.5
63	55920.0
64	42219.0
65	31071.0
66	22917.5
67	17467.0
68	13208.0
69	9622.0
70	6965.0
71	5010.5
72	3525.5
73	2346.5
74	1546.5
75	987.0
76	624.0
77	394.0
78	259.5
79	169.0
80	113.0
81	72.0
82	43.0
83	31.0
84	27.0
85	22.0
86	14.5
87	10.5
88	9.0
89	6.5
90	3.5
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003848772951460735
3	0.0
4	0.0
5	0.004034575783600219
6	6.635815433552993E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	2.6544568729064628E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.654036861101839E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	6.896448990828826E-6
48-49	4.169228136202846E-4
50-51	0.001504657299253522
52-53	0.0039239517025653065
54-55	0.0055741233591794885
56-57	0.0064141326914057915
58-59	0.007915728862683189
60-61	0.007482817254593504
62-63	0.005580465158066311
64-65	0.0034432563493315997
66-67	0.0012902202049967114
68-69	4.3359843641413496E-4
70-71	2.4124866691263976E-4
72-73	5.168772979661562E-4
74-75	1.532077372052197E-5
76-77	3.011691385960298E-4
78-79	2.2585541375037355E-4
80-81	6.285544755592877E-5
82-83	3.9629222652190484E-5
84-85	7.192883648633389E-5
86-87	6.447341539455917E-5
88-89	2.439433540763788E-5
90-91	1.6417540072957905E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	71.0
20-21	207.0
22-23	425.0
24-25	571.0
26-27	1254.0
28-29	3500.0
30-31	11348.0
32-33	14266.0
34-35	20927.0
36-37	34639.0
38-39	41888.0
40-41	41036.0
42-43	45924.0
44-45	54549.0
46-47	55340.0
48-49	52244.0
50-51	47982.0
52-53	46194.0
54-55	49501.0
56-57	51770.0
58-59	52302.0
60-61	51304.0
62-63	49160.0
64-65	51862.0
66-67	54594.0
68-69	55909.0
70-71	55271.0
72-73	51070.0
74-75	51683.0
76-77	54048.0
78-79	56052.0
80-81	56092.0
82-83	52831.0
84-85	51771.0
86-87	54172.0
88-89	57275.0
90-91	58570.0
92-93	57942.0
94-95	71037.0
96-97	234284.0
98-99	538540.0
100-101	5145464.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	84.90541717854973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.2223837887514	75.7546371724861
2	7.631099545411351	12.958433808683836
3	1.8351538880159952	4.674435193465068
4	0.6306145698685703	2.1417037253425066
5	0.2735112659773794	1.1611294070421339
6	0.1451917921500619	0.7396541810041372
7	0.08435952187305046	0.5013806278330037
8	0.04631620266348311	0.3145997207415432
9	0.03299290387708641	0.2521148640854231
>10	0.09581949223746364	1.2317819543639956
>50	0.0017079300264126978	0.09964390504510008
>100	8.00312365152267E-4	0.12599754462674131
>500	2.439339146593378E-5	0.01797741776411231
>1k	2.439339119667225E-5	0.026510477516198647
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	1.3271630867105984E-5
3	0.0	0.0	0.0	0.0	1.3271630867105984E-5
4	0.0	1.3271630867105984E-5	0.0	0.0	1.3271630867105984E-5
5	0.0	2.6543261734211967E-5	0.0	0.0	1.3271630867105984E-5
6	0.0	2.6543261734211967E-5	0.0	0.0	1.3271630867105984E-5
7	0.0	2.6543261734211967E-5	0.0	0.0	1.3271630867105984E-5
8	0.0	2.6543261734211967E-5	0.0	0.0	2.6543261734211967E-5
9	0.0	6.635815433552992E-5	0.0	0.0	2.6543261734211967E-5
10-11	0.0	6.635815433552992E-5	0.0	0.0	2.6543261734211967E-5
12-13	0.0	6.635815433552992E-5	0.0	0.0	2.6543261734211967E-5
14-15	0.0	1.3271630867105983E-4	0.0	1.3271630867105984E-5	3.9814892601317954E-5
16-17	0.0	1.3271630867105983E-4	0.0	2.6543261734211967E-5	5.3086523468423935E-5
18-19	0.0	1.5262375497171883E-4	0.0	2.6543261734211967E-5	5.3086523468423935E-5
20-21	0.0	1.9243864757303677E-4	0.0	3.9814892601317954E-5	5.3086523468423935E-5
22-23	1.3271630867105984E-5	2.322535401743547E-4	0.0	5.3086523468423935E-5	8.62656006361889E-5
24-25	1.3271630867105984E-5	2.521609864750137E-4	0.0	7.299396976908292E-5	1.2608049323750684E-4
26-27	1.3271630867105984E-5	2.720684327756727E-4	0.0	1.1280886237040086E-4	1.3935212410461285E-4
28-29	1.3271630867105984E-5	2.787042482092257E-4	0.0	2.2561772474080172E-4	1.4598793953816584E-4
30-31	1.3271630867105984E-5	3.0524750994343766E-4	0.0	8.95835083529654E-4	1.4598793953816584E-4
32-33	1.3271630867105984E-5	3.1851914081054364E-4	0.0	0.0022694488782751235	1.5925957040527182E-4
34-35	1.3271630867105984E-5	3.981489260131795E-4	0.0	0.00403457578360022	1.5925957040527182E-4
36-37	1.3271630867105984E-5	4.910503420829214E-4	0.0	0.007133501591069466	1.658953858388248E-4
38-39	1.3271630867105984E-5	5.109577883835804E-4	0.0	0.011971011042129599	1.725312012723778E-4
40-41	1.3271630867105984E-5	5.839517581526634E-4	0.0	0.01706068147966474	1.725312012723778E-4
42-43	1.3271630867105984E-5	6.436740970546403E-4	0.0	0.021918098377025533	1.725312012723778E-4
44-45	1.3271630867105984E-5	7.697545902921471E-4	0.0	0.027405917740573858	1.725312012723778E-4
46-47	2.6543261734211967E-5	8.626560063618889E-4	0.0	0.03353741120117682	1.725312012723778E-4
48-49	3.9814892601317954E-5	8.626560063618889E-4	0.0	0.04007368940322652	1.8580283213948378E-4
50-51	3.9814892601317954E-5	9.621932378651839E-4	0.0	0.046205182863829485	1.8580283213948378E-4
52-53	3.9814892601317954E-5	9.821006841658428E-4	0.0	0.0522172316466285	1.9243864757303677E-4
54-55	3.9814892601317954E-5	0.0010152797613336077	0.0	0.058302274399196594	1.9907446300658976E-4
56-57	3.9814892601317954E-5	0.0010617304693684786	0.0	0.06481864515494562	1.9907446300658976E-4
58-59	3.9814892601317954E-5	0.0010683662848020316	0.0	0.07102976840075123	1.9907446300658976E-4
60-61	3.9814892601317954E-5	0.0010750021002355846	0.0	0.07672993385817324	1.9907446300658976E-4
62-63	3.9814892601317954E-5	0.0010816379156691376	0.0	0.08236374116125975	1.9907446300658976E-4
64-65	3.9814892601317954E-5	0.0012608049323750686	0.0	0.08755294883029818	1.9907446300658976E-4
66-67	3.9814892601317954E-5	0.0012674407478086216	0.0	0.0923771866504912	1.9907446300658976E-4
68-69	3.9814892601317954E-5	0.0013006198249763865	0.0	0.09746685708802635	1.9907446300658976E-4
70-71	3.9814892601317954E-5	0.0013072556404099395	0.0	0.10195930413654172	1.9907446300658976E-4
72-73	3.9814892601317954E-5	0.0013205272712770455	0.0	0.10539002071568862	2.2561772474080172E-4
74-75	3.9814892601317954E-5	0.0013337989021441515	0.0	0.10813061248974601	2.2561772474080172E-4
76-77	3.9814892601317954E-5	0.0013802496101790224	0.0	0.11091101915640471	2.2561772474080172E-4
78-79	3.9814892601317954E-5	0.0014067928719132343	0.0	0.1126628744308627	2.2561772474080172E-4
80-81	3.9814892601317954E-5	0.0014067928719132343	0.0	0.11330654852791734	2.2561772474080172E-4
82-83	3.9814892601317954E-5	0.0014134286873467873	0.0	0.11368479000762986	2.2561772474080172E-4
84-85	3.9814892601317954E-5	0.0014200645027803403	0.0	0.11389050028607	2.2561772474080172E-4
86-87	3.9814892601317954E-5	0.0014466077645145523	0.0	0.11397676588670619	2.2561772474080172E-4
88	3.9814892601317954E-5	0.0014864226571158702	0.0	0.11399003751757329	2.2561772474080172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8580	0.0	46.68095	1
GTATCAA	15320	0.0	37.7193	1
TCAACGC	17205	0.0	33.25815	4
ATCAACG	17305	0.0	33.04097	3
CAACGCA	17735	0.0	32.264248	5
AACGCAG	18415	0.0	31.117962	6
TATCAAC	18935	0.0	30.495232	2
ACGCAGA	21135	0.0	27.113186	7
CGCAGAG	21410	0.0	26.764933	8
GCAGAGT	25605	0.0	22.329226	9
TACATGG	19395	0.0	21.810469	2
GTACATG	19655	0.0	21.720015	1
ACATGGG	19600	0.0	20.875072	3
GAGTACT	14830	0.0	20.39922	12-13
GTGGTAT	4805	0.0	20.163717	1
TGGTATC	4635	0.0	19.690132	2
CAGAGTA	25285	0.0	19.113998	10-11
AGAGTAC	22960	0.0	18.31828	10-11
GTACTTT	16730	0.0	18.160425	14-15
CATGGGG	14375	0.0	17.992283	4
>>END_MODULE