FastQCFastQC Report
Fri 27 May 2016
SRR2139783_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139783_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7201562
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA80950.043.287231
GTATCAA137650.033.291791
TCAACGC157300.028.9414464
ATCAACG160100.028.4619143
CAACGCA164450.027.6053645
AACGCAG169600.026.8419516
TATCAAC173650.026.340852
ACGCAGA198800.022.8136087
CGCAGAG201950.022.4366558
GTGGTAT46250.020.1853941
TACATGG179100.018.8272022
GTACATG183250.018.7265071
TGGTATC45400.018.6853872
GCAGAGT247650.018.3823749
ACATGGG185650.017.7255653
GAGTACT140650.017.4108112-13
AGAGTAC214200.017.36259510-11
CAGAGTA235900.016.93091210-11
TCGCGCG3758.039933E-1015.9134899
CATGGGG133900.015.1850254
Produced by FastQC (version 0.11.5)