##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139783_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7201562
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.969577294481393	31.0	25.0	33.0	18.0	33.0
2	30.661605079564684	32.0	29.0	33.0	27.0	33.0
3	32.04640465499013	33.0	32.0	33.0	30.0	34.0
4	31.666427366729607	33.0	31.0	33.0	29.0	34.0
5	32.610675017447605	33.0	33.0	34.0	32.0	34.0
6	36.105316318876376	38.0	36.0	38.0	33.0	38.0
7	36.81944167112635	38.0	37.0	38.0	35.0	38.0
8	37.03110297460468	38.0	38.0	38.0	36.0	38.0
9	37.12591212850768	38.0	38.0	38.0	36.0	38.0
10-11	37.184468869392504	38.0	38.0	38.0	36.0	38.0
12-13	37.20588651462002	38.0	38.0	38.0	36.0	38.0
14-15	37.21491032084428	38.0	38.0	38.0	36.0	38.0
16-17	37.180085375922616	38.0	38.0	38.0	36.0	38.0
18-19	37.20470371566613	38.0	38.0	38.0	36.0	38.0
20-21	37.22678735706087	38.0	38.0	38.0	36.0	38.0
22-23	37.23288544443976	38.0	38.0	38.0	36.0	38.0
24-25	37.238630082126335	38.0	38.0	38.0	36.0	38.0
26-27	37.20904837041718	38.0	38.0	38.0	36.0	38.0
28-29	37.18129737804681	38.0	38.0	38.0	36.0	38.0
30-31	37.18399961493713	38.0	38.0	38.0	36.0	38.0
32-33	37.17611221349535	38.0	38.0	38.0	36.0	38.0
34-35	37.15763895540066	38.0	38.0	38.0	36.0	38.0
36-37	37.144141091012656	38.0	38.0	38.0	36.0	38.0
38-39	37.130137584765976	38.0	38.0	38.0	36.0	38.0
40-41	37.11348815853378	38.0	38.0	38.0	36.0	38.0
42-43	37.0956221486596	38.0	38.0	38.0	36.0	38.0
44-45	37.056491473405075	38.0	38.0	38.0	36.0	38.0
46-47	37.04131659062291	38.0	38.0	38.0	36.0	38.0
48-49	37.0183768338117	38.0	38.0	38.0	36.0	38.0
50-51	36.98520179907021	38.0	38.0	38.0	36.0	38.0
52-53	36.96173987746954	38.0	38.0	38.0	36.0	38.0
54-55	36.93717466855793	38.0	38.0	38.0	36.0	38.0
56-57	36.936254796897714	38.0	38.0	38.0	36.0	38.0
58-59	36.91790001395349	38.0	38.0	38.0	36.0	38.0
60-61	36.92761130329082	38.0	38.0	38.0	36.0	38.0
62-63	36.91487283073787	38.0	38.0	38.0	35.5	38.0
64-65	36.90569544918611	38.0	38.0	38.0	35.0	38.0
66-67	36.89245175550552	38.0	38.0	38.0	35.0	38.0
68-69	36.89644394900859	38.0	38.0	38.0	35.0	38.0
70-71	36.87375959438673	38.0	38.0	38.0	35.0	38.0
72-73	36.858935386774604	38.0	38.0	38.0	35.0	38.0
74-75	36.8700244022119	38.0	38.0	38.0	35.0	38.0
76-77	36.849431875607166	38.0	38.0	38.0	35.0	38.0
78-79	36.8242424117654	38.0	38.0	38.0	35.0	38.0
80-81	36.81420997158599	38.0	38.0	38.0	35.0	38.0
82-83	36.75974135006285	38.0	38.0	38.0	35.0	38.0
84-85	36.75897892741378	38.0	38.0	38.0	35.0	38.0
86-87	36.74737594194559	38.0	38.0	38.0	35.0	38.0
88-89	36.72190550111219	38.0	38.0	38.0	35.0	38.0
90-91	36.69950206856592	38.0	38.0	38.0	34.0	38.0
92-93	36.69841465219907	38.0	38.0	38.0	34.0	38.0
94-95	36.64092590994851	38.0	38.0	38.0	34.0	38.0
96-97	36.60515064291927	38.0	38.0	38.0	34.0	38.0
98-99	36.62761719048423	38.0	38.0	38.0	34.0	38.0
100	35.00539700740812	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	16.0
19	41.0
20	79.0
21	224.0
22	577.0
23	1715.0
24	4275.0
25	8513.0
26	15249.0
27	24463.0
28	37222.0
29	52970.0
30	72911.0
31	96599.0
32	128476.0
33	172667.0
34	251171.0
35	432194.0
36	1155814.0
37	4746384.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.92382485910696	21.432267055397148	13.991978406906725	24.651929678589173
2	14.049588268784802	21.590990515601348	40.9105578159499	23.44886339966395
3	18.767720114053034	25.219362132826184	30.17374564018195	25.83917211293883
4	12.831855089215368	17.60525008324583	38.79716650360019	30.765728323938614
5	13.4349196068877	37.788338523830625	35.325019742960336	13.451722126321341
6	30.58640807436727	37.923295732657735	18.338636586126192	13.151659606848797
7	26.980785557355475	32.575752315955896	22.549538558440517	17.89392356824811
8	24.42806157886303	37.168200454290336	20.78510189872697	17.618636068119667
9	25.10617835408485	17.16081872238273	21.247334953167105	36.48566797036531
10-11	24.28750873768774	26.99735140793067	28.69748257391938	20.017657280462213
12-13	24.89023214685925	24.235804676818724	29.032729288451588	21.841233887870438
14-15	21.888133435496353	25.90235979361144	26.566042200289324	25.643464570602877
16-17	21.690690158607257	29.105345479216872	27.27758922300468	21.9263751391712
18-19	21.640937063375972	28.28899480418276	28.975949939749185	21.094118192692086
20-21	22.363557818924697	27.374141093948072	28.939025811026852	21.32327527610038
22-23	22.053049036840207	27.34760717619747	28.78961640595084	21.80972738101148
24-25	21.854026656315867	27.3891788092392	28.913627884385374	21.84316665005956
26-27	21.826716201349626	27.573025995708655	28.777992945349308	21.822264857592415
28-29	21.778862558504247	27.659310497365297	28.78790341672585	21.773923527404605
30-31	22.04844004912539	27.45436772198734	28.758365633699317	21.738826595187955
32-33	21.825887231825554	27.6018900021007	28.720116858789474	21.85210590728427
34-35	21.96269787618524	27.60682556396114	28.734632585837367	21.695843974016256
36-37	21.927002288538155	27.5259360293586	28.73027476274239	21.816786919360855
38-39	21.792241161964967	27.664140026942263	28.808836881333317	21.734781929759457
40-41	22.094198580249383	27.536478864745067	28.622208539004852	21.747114016000697
42-43	21.914523699407003	27.592866960399803	28.53024893751242	21.962360402680773
44-45	22.039013596739377	27.647796520993325	28.428356751341475	21.884833130925827
46-47	22.01141275259017	27.654849416423243	28.26060701115208	22.073130819834503
48-49	21.946950856620116	27.734786262685297	28.31955057241256	21.998712308282023
50-51	21.95225200540024	27.894611701338928	28.201379790801617	21.95175650245921
52-53	22.124944315052222	27.84212358633647	28.062932990762988	21.969999107848317
54-55	22.05164724011554	27.748307564154494	28.145595760255887	22.05444943547408
56-57	22.04438149161582	27.860872295561812	28.118307405070613	21.976438807751755
58-59	22.051419284585972	27.89603811685003	28.119890683266274	21.932651915297726
60-61	22.09190064462524	27.838557811329608	28.20076765227362	21.868773891771536
62-63	22.072569634552337	27.8391744978695	28.15686139686874	21.93139447070942
64-65	22.04787811508494	27.824849893570025	28.12561184686132	22.001660144483715
66-67	22.064769396350595	27.844799274427995	27.987065656334988	22.103365672886426
68-69	22.080931479041492	27.815376496778164	28.060412979765893	22.04327904441445
70-71	22.057954641632687	27.642633725080263	28.09494421598076	22.20446741730629
72-73	22.10438561491529	27.683724319838714	28.06453433581731	22.14735572942869
74-75	21.99558082281575	27.79359355733079	28.022080435402202	22.188745184451257
76-77	22.07830939001573	27.755560979097993	27.966611809986723	22.19951782089955
78-79	22.075913787937793	27.769322208735016	27.948282749611415	22.206481253715776
80-81	22.05579813373163	27.78685988362567	27.92632728892765	22.231014693715053
82-83	22.115950698774256	27.785695949445582	27.9690757332349	22.12927761854526
84-85	22.24225939696409	27.71912583907707	27.953973341830768	22.084641422128072
86-87	22.075644311668764	27.77154483937539	27.972800043646544	22.180010805309298
88-89	22.161938839447828	27.782957280316207	27.921782428254886	22.133321451981082
90-91	22.180721506003056	27.791284647836523	27.935143643590006	22.092850202570414
92-93	22.17422122953122	27.715252720817713	28.008717197813738	22.10180885183733
94-95	22.08955859424474	27.850235981243788	27.9557524249584	22.104452999553075
96-97	22.185320138442442	27.942904972712178	28.019738346815	21.85203654203038
98-99	23.23262021981441	29.85474470053491	28.217773379898954	18.69486169975173
100	22.104137360381582	30.778755464340513	22.125024364648198	24.99208281062971
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	655.0
1	689.0
2	952.5
3	1381.5
4	1974.0
5	2817.0
6	3544.0
7	4494.0
8	5593.5
9	6196.5
10	6342.5
11	6494.5
12	7350.5
13	9437.0
14	13394.0
15	17892.0
16	20968.5
17	22495.0
18	22411.0
19	21115.0
20	19712.5
21	18915.0
22	19788.5
23	23050.0
24	28858.0
25	37656.0
26	49753.5
27	63910.5
28	79089.0
29	96619.0
30	113966.5
31	131682.5
32	151633.5
33	171308.0
34	190675.5
35	209949.0
36	230489.5
37	249391.5
38	262997.5
39	274269.0
40	286261.5
41	297548.0
42	305142.5
43	311262.5
44	316679.5
45	320940.0
46	322316.5
47	320383.0
48	316955.0
49	309750.0
50	299887.5
51	286863.5
52	271116.5
53	254589.5
54	237571.0
55	217762.5
56	196657.0
57	175118.0
58	153098.5
59	131824.5
60	109393.0
61	88454.0
62	69733.5
63	53778.5
64	40821.0
65	30082.0
66	21907.0
67	16564.5
68	12733.5
69	9318.5
70	6635.5
71	4743.0
72	3399.5
73	2316.0
74	1514.0
75	954.5
76	598.5
77	364.5
78	223.5
79	151.5
80	115.5
81	86.5
82	58.5
83	39.0
84	27.0
85	20.5
86	15.0
87	10.0
88	6.0
89	4.5
90	4.0
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00363809962338726
3	0.0
4	0.0
5	0.0036242137469621174
6	9.720113497599549E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	6.943318083955269E-6
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	6.967492328442571E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	7.271213802625229E-6
48-49	3.0805927060366416E-4
50-51	0.0015974167995443812
52-53	0.004181087443456568
54-55	0.005957127462620527
56-57	0.007121843915092771
58-59	0.008682457019162702
60-61	0.008250991140671765
62-63	0.006058886465334303
64-65	0.003639571936139318
66-67	0.0012031508303917483
68-69	4.5547092497355074E-4
70-71	3.0659553972877106E-4
72-73	5.375307644738099E-4
74-75	0.0
76-77	2.073919983355548E-4
78-79	2.0107885508380968E-4
80-81	5.9243355709548786E-5
82-83	0.0
84-85	1.1220191511407266E-4
86-87	1.743069924383012E-4
88-89	2.641604299122943E-5
90-91	8.901063169688178E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	67.0
20-21	237.0
22-23	433.0
24-25	518.0
26-27	1588.0
28-29	4966.0
30-31	13701.0
32-33	17317.0
34-35	24272.0
36-37	40420.0
38-39	47948.0
40-41	46521.0
42-43	51762.0
44-45	59800.0
46-47	60753.0
48-49	56916.0
50-51	52827.0
52-53	52008.0
54-55	55741.0
56-57	57415.0
58-59	57738.0
60-61	56510.0
62-63	53937.0
64-65	56667.0
66-67	59376.0
68-69	60113.0
70-71	58810.0
72-73	55532.0
74-75	56064.0
76-77	58770.0
78-79	59782.0
80-81	59759.0
82-83	56282.0
84-85	55851.0
86-87	57991.0
88-89	60594.0
90-91	60748.0
92-93	59996.0
94-95	71345.0
96-97	220081.0
98-99	490192.0
100-101	4730214.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.32404809548846
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.40163043093101	76.28109014703841
2	7.4906906142599965	12.78272092479088
3	1.831895262939796	4.689141585629178
4	0.6280909801406149	2.1436505999144124
5	0.267884548681698	1.142849705787771
6	0.13499933019941168	0.6911213605675998
7	0.07601128732084272	0.45399135146145025
8	0.04935173775471298	0.3368712036623251
9	0.027583008779629062	0.21181445709582153
>10	0.09033323133921731	1.1287504242512902
>50	0.0011368209556060814	0.06259261386740347
>100	3.678874374085295E-4	0.05769450191852321
>500	2.4859260006262194E-5	0.01771112401487794
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	1.3885876425142212E-5	0.0	0.0	0.0
3	0.0	1.3885876425142212E-5	0.0	0.0	0.0
4	0.0	6.942938212571107E-5	0.0	2.7771752850284425E-5	0.0
5	1.3885876425142212E-5	6.942938212571107E-5	0.0	2.7771752850284425E-5	0.0
6	1.3885876425142212E-5	6.942938212571107E-5	0.0	2.7771752850284425E-5	2.7771752850284425E-5
7	1.3885876425142212E-5	6.942938212571107E-5	0.0	2.7771752850284425E-5	2.7771752850284425E-5
8	1.3885876425142212E-5	6.942938212571107E-5	0.0	2.7771752850284425E-5	2.7771752850284425E-5
9	2.7771752850284425E-5	1.8051639352684875E-4	0.0	2.7771752850284425E-5	2.7771752850284425E-5
10-11	6.942938212571107E-5	1.9440226995199098E-4	0.0	2.7771752850284425E-5	2.7771752850284425E-5
12-13	1.110870114011377E-4	2.0828814637713317E-4	0.0	5.554350570056885E-5	2.7771752850284425E-5
14-15	1.1802994961370881E-4	2.9160340492798647E-4	0.0	5.554350570056885E-5	2.7771752850284425E-5
16-17	1.31915826038851E-4	2.9160340492798647E-4	0.0	5.554350570056885E-5	2.7771752850284425E-5
18-19	1.3885876425142213E-4	3.193751577782709E-4	0.0	5.554350570056885E-5	2.7771752850284425E-5
20-21	1.8051639352684875E-4	3.5408984884112643E-4	0.0	5.554350570056885E-5	2.7771752850284425E-5
22-23	1.8051639352684875E-4	3.95747478116553E-4	0.0	6.942938212571107E-5	2.7771752850284425E-5
24-25	1.8051639352684875E-4	4.58233922029693E-4	0.0	9.025819676342439E-5	2.7771752850284425E-5
26-27	1.8051639352684875E-4	4.8600567487997744E-4	0.0	2.0134520816456208E-4	2.7771752850284425E-5
28-29	1.9440226995199098E-4	4.8600567487997744E-4	0.0	4.58233922029693E-4	2.7771752850284425E-5
30-31	2.430028374399887E-4	5.554350570056885E-4	0.0	0.0014441311482147902	2.7771752850284425E-5
32-33	2.63831652077702E-4	5.623779952182596E-4	0.0	0.003332610342034131	2.7771752850284425E-5
34-35	2.8466046671541534E-4	7.081796976822529E-4	0.0	0.005845953974984872	2.7771752850284425E-5
36-37	3.0548928135312866E-4	8.331525855085327E-4	0.0	0.009949230458614395	2.7771752850284425E-5
38-39	3.0548928135312866E-4	8.609243383588172E-4	0.0	0.01589238556857526	2.7771752850284425E-5
40-41	3.0548928135312866E-4	9.025819676342438E-4	0.0	0.02209242939240126	4.165762927542664E-5
42-43	3.0548928135312866E-4	9.372966586970994E-4	0.0	0.028091128008062696	4.8600567487997744E-5
44-45	3.193751577782709E-4	0.0010969842375862346	0.0	0.03501323740599609	6.248644391313996E-5
46-47	3.6103278705369756E-4	0.0012080712489873724	0.0	0.042830985833351154	6.942938212571107E-5
48-49	3.8880453990398195E-4	0.0012150141871999434	0.0	0.05068344895176907	6.942938212571107E-5
50-51	3.8880453990398195E-4	0.0013885876425142212	0.0	0.058799743722264694	6.942938212571107E-5
52-53	4.0269041632912415E-4	0.0014024735189393635	0.0	0.06647863338536834	9.720113497599549E-5
54-55	4.1657629275426635E-4	0.0014094164571519345	0.0	0.07440052588591198	1.249728878262799E-4
56-57	4.304621691794086E-4	0.0014580170246399323	0.0	0.08217661668399162	1.249728878262799E-4
58-59	4.443480456045508E-4	0.0014857887774902168	0.0	0.08901541082337415	1.249728878262799E-4
60-61	4.443480456045508E-4	0.0015343893449782145	0.0	0.0960902648619841	1.249728878262799E-4
62-63	4.58233922029693E-4	0.0015552181596159278	0.0	0.1025333115232501	1.3885876425142213E-4
64-65	4.8600567487997744E-4	0.001763506305993061	0.0	0.10862921127388753	1.3885876425142213E-4
66-67	4.8600567487997744E-4	0.0018051639352684877	0.0	0.11503754324409066	1.3885876425142213E-4
68-69	4.998915513051196E-4	0.0018190498116936297	0.0	0.12148058990535665	1.3885876425142213E-4
70-71	5.137774277302619E-4	0.0018259927499062007	0.0	0.1270696551664764	1.4580170246399323E-4
72-73	5.137774277302619E-4	0.0018537645027564852	0.0	0.13142982036397105	1.5274464067656433E-4
74-75	5.137774277302619E-4	0.0019231938848821963	0.0	0.13503320529629545	1.5274464067656433E-4
76-77	5.137774277302619E-4	0.0019856803287953365	0.0	0.138213070997653	1.5968757888913546E-4
78-79	5.27663304155404E-4	0.0019995662052204786	0.0	0.14031678127606206	1.6663051710170656E-4
80-81	5.415491805805463E-4	0.0020134520816456206	0.0	0.14088610220949288	1.7357345531427765E-4
82-83	5.415491805805463E-4	0.0020134520816456206	0.0	0.14126796381118428	1.8051639352684875E-4
84-85	5.484921187931174E-4	0.0020412238344959055	0.0	0.1415595672161123	1.8051639352684875E-4
86-87	5.554350570056885E-4	0.002075938525558761	0.0	0.14167759716572598	1.8051639352684875E-4
88	5.554350570056885E-4	0.002110653216621616	0.0	0.14169148304215112	1.9440226995199098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8095	0.0	43.28723	1
GTATCAA	13765	0.0	33.29179	1
TCAACGC	15730	0.0	28.941446	4
ATCAACG	16010	0.0	28.461914	3
CAACGCA	16445	0.0	27.605364	5
AACGCAG	16960	0.0	26.841951	6
TATCAAC	17365	0.0	26.34085	2
ACGCAGA	19880	0.0	22.813608	7
CGCAGAG	20195	0.0	22.436655	8
GTGGTAT	4625	0.0	20.185394	1
TACATGG	17910	0.0	18.827202	2
GTACATG	18325	0.0	18.726507	1
TGGTATC	4540	0.0	18.685387	2
GCAGAGT	24765	0.0	18.382374	9
ACATGGG	18565	0.0	17.725565	3
GAGTACT	14065	0.0	17.41081	12-13
AGAGTAC	21420	0.0	17.362595	10-11
CAGAGTA	23590	0.0	16.930912	10-11
TCGCGCG	375	8.039933E-10	15.913489	9
CATGGGG	13390	0.0	15.185025	4
>>END_MODULE