FastQCFastQC Report
Fri 27 May 2016
SRR2139782_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139782_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8095361
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA86650.046.639191
GTATCAA151850.033.5156141
TCAACGC175300.029.941434
ATCAACG179150.029.2739033
CAACGCA181600.028.9502125
AACGCAG188650.027.9130066
TATCAAC191250.027.693442
ACGCAGA227950.023.1573897
CGCAGAG234000.022.5402348
TGGTATC47450.019.5425952
GAGTACT149600.019.32924812-13
GCAGAGT280850.018.8416049
GTGGTAT50900.018.7264061
TACATGG219350.018.2076262
GTACATG216100.018.1022471
CAGAGTA272550.017.53239810-11
ACATGGG221500.017.3682083
GTACTTT169350.017.13890314-15
AGAGTAC248500.016.49580410-11
CATGGGG165100.015.0727544
Produced by FastQC (version 0.11.5)