##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139782_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8095361
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.268934887523855	25.0	18.0	32.0	18.0	33.0
2	28.25808879925182	30.0	25.0	32.0	18.0	33.0
3	29.181808445602364	32.0	27.0	33.0	18.0	33.0
4	31.133863085290454	33.0	31.0	33.0	28.0	33.0
5	31.809035940460223	33.0	32.0	33.0	30.0	33.0
6	36.34424480884793	38.0	37.0	38.0	34.0	38.0
7	36.47542747012764	38.0	37.0	38.0	34.0	38.0
8	36.986215315166305	38.0	38.0	38.0	35.0	38.0
9	37.1025556488463	38.0	38.0	38.0	36.0	38.0
10-11	37.13787192442685	38.0	38.0	38.0	36.0	38.0
12-13	37.1754727182642	38.0	38.0	38.0	36.0	38.0
14-15	37.18760756932273	38.0	38.0	38.0	36.0	38.0
16-17	37.15360185913883	38.0	38.0	38.0	36.0	38.0
18-19	37.17729765232211	38.0	38.0	38.0	36.0	38.0
20-21	37.19684952314421	38.0	38.0	38.0	36.0	38.0
22-23	37.20290323830231	38.0	38.0	38.0	36.0	38.0
24-25	37.20422542711557	38.0	38.0	38.0	36.0	38.0
26-27	37.1757146848541	38.0	38.0	38.0	36.0	38.0
28-29	37.14985131225812	38.0	38.0	38.0	36.0	38.0
30-31	37.15086740539773	38.0	38.0	38.0	36.0	38.0
32-33	37.144462421224475	38.0	38.0	38.0	36.0	38.0
34-35	37.127649888994014	38.0	38.0	38.0	36.0	38.0
36-37	37.11630994059303	38.0	38.0	38.0	36.0	38.0
38-39	37.099122272344765	38.0	38.0	38.0	36.0	38.0
40-41	37.07761919400292	38.0	38.0	38.0	36.0	38.0
42-43	37.05134809668737	38.0	38.0	38.0	36.0	38.0
44-45	37.009922873181196	38.0	38.0	38.0	36.0	38.0
46-47	36.99372055196086	38.0	38.0	38.0	36.0	38.0
48-49	36.96796772315308	38.0	38.0	38.0	36.0	38.0
50-51	36.93131280707724	38.0	38.0	38.0	36.0	38.0
52-53	36.90342745547498	38.0	38.0	38.0	35.5	38.0
54-55	36.87202545404677	38.0	38.0	38.0	35.0	38.0
56-57	36.87212779392239	38.0	38.0	38.0	35.0	38.0
58-59	36.853549129569046	38.0	38.0	38.0	35.0	38.0
60-61	36.85909140166922	38.0	38.0	38.0	35.0	38.0
62-63	36.84167583770417	38.0	38.0	38.0	35.0	38.0
64-65	36.82984472327065	38.0	38.0	38.0	35.0	38.0
66-67	36.81417860782278	38.0	38.0	38.0	35.0	38.0
68-69	36.81595603239589	38.0	38.0	38.0	35.0	38.0
70-71	36.79201558999179	38.0	38.0	38.0	35.0	38.0
72-73	36.77123580142516	38.0	38.0	38.0	35.0	38.0
74-75	36.77988038533654	38.0	38.0	38.0	35.0	38.0
76-77	36.75314329767171	38.0	38.0	38.0	35.0	38.0
78-79	36.72407307516079	38.0	38.0	38.0	35.0	38.0
80-81	36.714003664945096	38.0	38.0	38.0	34.5	38.0
82-83	36.65443561874087	38.0	38.0	38.0	34.0	38.0
84-85	36.646195539424035	38.0	38.0	38.0	34.0	38.0
86-87	36.62911991481644	38.0	38.0	38.0	34.0	38.0
88-89	36.59910453431493	38.0	38.0	38.0	34.0	38.0
90-91	36.57639433059641	38.0	38.0	38.0	34.0	38.0
92-93	36.57362871145568	38.0	38.0	38.0	34.0	38.0
94-95	36.51187403254373	38.0	38.0	38.0	34.0	38.0
96-97	36.47353546123086	38.0	38.0	38.0	34.0	38.0
98-99	36.496845500348485	38.0	38.0	38.0	34.0	38.0
100	34.81203455189656	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	13.0
18	32.0
19	61.0
20	103.0
21	275.0
22	850.0
23	2313.0
24	5508.0
25	10734.0
26	19015.0
27	30875.0
28	45208.0
29	64899.0
30	88558.0
31	118024.0
32	155491.0
33	213760.0
34	318094.0
35	568651.0
36	1635615.0
37	4817282.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.32225159569783	23.768254436090004	14.002006828355155	24.90748713985701
2	14.317601414490374	19.6490555796237	40.306996689714026	25.726346316171906
3	17.56489920585382	24.352206158564147	31.254430778318593	26.82846385726344
4	13.073166224458674	17.410810957040702	37.99917014201096	31.51685267648966
5	13.789981052553038	36.47570074547049	35.388822908696234	14.34549529328024
6	31.13230050185594	37.50114108702637	17.917370105385178	13.449188305732513
7	27.34034961504496	32.23701821327054	22.264998435523754	18.157633736160747
8	24.658010927492917	37.235300562877924	20.277675078356605	17.829013431272553
9	25.173046637450756	16.96843414394985	20.970491618594895	36.8880276000045
10-11	24.52957934797472	26.78475981491128	28.319638864777	20.366021972337
12-13	25.04454711778758	23.963637940296916	28.78119950425929	22.21061543765621
14-15	22.123139412806914	25.633291708671173	26.138154926012565	26.105413952509345
16-17	21.98241066704746	28.86358619461195	26.92653236835269	22.227470769987896
18-19	21.950713501226197	28.039910758766656	28.587434210778245	21.4219415292289
20-21	22.64722184580116	27.104811723518324	28.582036204198292	21.665930226482224
22-23	22.393263679907438	26.99600931861072	28.46595672492723	22.14477027655461
24-25	22.188611181889076	27.118179642198083	28.5295221329133	22.163687042999545
26-27	22.147300507911485	27.28392140777101	28.47011278822525	22.098665296092257
28-29	22.104072143754617	27.31922815545199	28.48837820031877	22.08832150047462
30-31	22.34746457521145	27.178245465130452	28.430074598910952	22.044215360747145
32-33	22.072202781541943	27.30460856665557	28.44475356248074	22.178435089321745
34-35	22.257761420433216	27.327671745546816	28.404057234617962	22.010509599402006
36-37	22.24119975264759	27.259768317396134	28.377327690541577	22.1217042394147
38-39	22.076488397355117	27.398491498943734	28.459825357434976	22.065194746266172
40-41	22.36639488102674	27.25733936521133	28.32319151078232	22.053074242979612
42-43	22.216451362041294	27.30932207686076	28.23275687256217	22.241469688535776
44-45	22.248374401264357	27.380474415357696	28.155837752680846	22.215313430697105
46-47	22.287802009537053	27.407759698079516	27.97812339221541	22.32631490016803
48-49	22.208160447036164	27.461513098410766	28.03806099890523	22.292265455647843
50-51	22.23961976971408	27.54793848852013	27.922946246930003	22.289495494835787
52-53	22.423740039978078	27.47938999917205	27.80891345573827	22.287956505111595
54-55	22.32357331204418	27.437376929519836	27.88828937052822	22.350760387907766
56-57	22.27905983091141	27.54912864417507	27.88254652069247	22.289265004221047
58-59	22.27370160745414	27.55574447401744	27.87776193331655	22.292791985211878
60-61	22.34937920290293	27.53217338926926	27.940069774814198	22.178377633013614
62-63	22.355858858197543	27.546741128476153	27.885204725532336	22.21219528779397
64-65	22.30261239036312	27.511535254713472	27.85533754812583	22.33051480679758
66-67	22.326127100952075	27.580197141552752	27.77548540192897	22.31819035556619
68-69	22.31324154175876	27.50611503073997	27.824975193479684	22.355668234021582
70-71	22.295782059343146	27.36023527252337	27.898337916599374	22.445644751534108
72-73	22.339144650388217	27.42596857197659	27.81099498639778	22.423891791237413
74-75	22.212413813640254	27.558869003318787	27.766472894921808	22.462244288119155
76-77	22.34965816127593	27.465271527163544	27.736525127336893	22.448545184223637
78-79	22.2865265767938	27.470889201705578	27.72505417761608	22.51753004388454
80-81	22.36649596852936	27.500385345498056	27.647786427051564	22.485332258921023
82-83	22.403597033889447	27.502824404814408	27.723825464901996	22.369753096394145
84-85	22.414417646816986	27.4933865548417	27.72824888530092	22.36394691304039
86-87	22.319374303670678	27.523360251407585	27.754139375784348	22.40312606913739
88-89	22.373097447900854	27.498978440627564	27.7128459179583	22.41507819351328
90-91	22.402481641691974	27.574345329286672	27.67994890105155	22.34322412796981
92-93	22.40973107264927	27.504994626540284	27.725206434999127	22.360067865811317
94-95	22.33490862224881	27.577829360353	27.737609298839878	22.349652718558314
96-97	22.418932235867732	27.702746548047415	27.762882157743928	22.115439058340925
98-99	23.486299968537296	29.555541992397373	27.995662272776507	18.96249576628883
100	22.33355449313904	30.47334569279902	21.947607879113868	25.24549193494807
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	914.0
1	928.0
2	1251.0
3	1682.5
4	2060.0
5	2657.0
6	3330.5
7	4350.5
8	5606.5
9	6574.5
10	7008.0
11	7442.5
12	8614.0
13	10653.0
14	14386.0
15	18741.5
16	21928.5
17	23565.0
18	23376.5
19	21912.0
20	20244.0
21	19239.5
22	19966.0
23	23396.0
24	29620.5
25	38756.5
26	50925.5
27	65458.0
28	81021.0
29	98431.0
30	117053.5
31	136717.0
32	157329.5
33	177517.5
34	198438.5
35	218329.5
36	239436.0
37	259785.5
38	277026.5
39	292333.5
40	306648.5
41	318650.5
42	327230.5
43	335178.0
44	340986.5
45	346769.0
46	350774.0
47	350532.0
48	347895.0
49	341827.5
50	334458.5
51	324301.0
52	310281.5
53	294196.0
54	276614.0
55	258138.0
56	237469.5
57	214883.5
58	190441.5
59	164486.0
60	137835.5
61	112038.0
62	88862.5
63	69392.0
64	53039.5
65	39598.0
66	29481.0
67	22672.0
68	17501.0
69	12905.5
70	9420.5
71	6787.5
72	4649.5
73	3148.0
74	2122.0
75	1377.5
76	888.0
77	548.0
78	355.5
79	224.5
80	149.0
81	104.5
82	74.5
83	47.0
84	28.5
85	19.0
86	13.5
87	12.0
88	8.5
89	4.0
90	4.5
91	2.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0035822985534554913
3	0.0
4	0.0
5	0.004187583481453144
6	1.2352753632605143E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	3.097512096868867E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.9288589249941988E-5
48-49	4.340968427294351E-4
50-51	0.0014814998521763363
52-53	0.003916173903353033
54-55	0.0057956919351557345
56-57	0.00654528037628297
58-59	0.00821450179953338
60-61	0.00785864457672298
62-63	0.00563983252084283
64-65	0.0031055160216800744
66-67	0.001087308444127409
68-69	5.866372416875597E-4
70-71	2.817357145729841E-4
72-73	6.320133958434822E-4
74-75	2.8633036640193753E-5
76-77	2.742844208181298E-4
78-79	2.7668497326094576E-4
80-81	1.028566525037954E-4
82-83	1.4824324344357196E-5
84-85	1.345485760724194E-4
86-87	1.3570345959384257E-4
88-89	3.803980576570858E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	70.0
20-21	228.0
22-23	416.0
24-25	512.0
26-27	1474.0
28-29	4821.0
30-31	13080.0
32-33	16703.0
34-35	23791.0
36-37	40058.0
38-39	46750.0
40-41	45313.0
42-43	50563.0
44-45	59587.0
46-47	60349.0
48-49	57126.0
50-51	52656.0
52-53	51087.0
54-55	54983.0
56-57	57337.0
58-59	57460.0
60-61	55763.0
62-63	54040.0
64-65	56779.0
66-67	59760.0
68-69	60914.0
70-71	58677.0
72-73	56031.0
74-75	57277.0
76-77	59284.0
78-79	61478.0
80-81	60840.0
82-83	57047.0
84-85	56433.0
86-87	59320.0
88-89	61734.0
90-91	62807.0
92-93	62902.0
94-95	76957.0
96-97	252296.0
98-99	576893.0
100-101	5493765.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.98467466654283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.2622361193623	77.61169007398038
2	6.896107507165272	11.859191209382193
3	1.5910773717574698	4.104248105395921
4	0.5805872621257679	1.996864273976919
5	0.2576502050581161	1.107698452984508
6	0.13939922627768866	0.7191718272152907
7	0.0788476947321195	0.4745785365823724
8	0.05297230100061127	0.36438448543005925
9	0.035616870760909906	0.2756254540515426
>10	0.10347122371174461	1.3238449739818172
>50	0.001557718366766291	0.08867287490586748
>100	4.541769064428616E-4	0.06139997135008866
>500	2.2322774805848326E-5	0.012629760763026717
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.4705507265210287E-5
2	0.0	0.0	0.0	0.0	2.4705507265210287E-5
3	0.0	1.2352753632605143E-5	0.0	0.0	2.4705507265210287E-5
4	0.0	3.7058260897815426E-5	0.0	0.0	3.7058260897815426E-5
5	0.0	3.7058260897815426E-5	0.0	0.0	3.7058260897815426E-5
6	0.0	3.7058260897815426E-5	0.0	0.0	3.7058260897815426E-5
7	0.0	3.7058260897815426E-5	0.0	1.2352753632605143E-5	3.7058260897815426E-5
8	0.0	3.7058260897815426E-5	0.0	3.7058260897815426E-5	3.7058260897815426E-5
9	0.0	7.411652179563085E-5	0.0	6.176376816302571E-5	3.7058260897815426E-5
10-11	0.0	7.411652179563085E-5	0.0	8.029289861193342E-5	3.7058260897815426E-5
12-13	0.0	8.029289861193342E-5	0.0	8.646927542823599E-5	3.7058260897815426E-5
14-15	0.0	1.1735115950974886E-4	0.0	9.264565224453858E-5	3.7058260897815426E-5
16-17	1.2352753632605143E-5	1.2352753632605143E-4	0.0	1.1735115950974886E-4	4.941101453042057E-5
18-19	1.2352753632605143E-5	1.482330435912617E-4	0.0	1.2352753632605143E-4	4.941101453042057E-5
20-21	1.2352753632605143E-5	1.8529130448907715E-4	0.0	1.2352753632605143E-4	4.941101453042057E-5
22-23	1.2352753632605143E-5	2.1617318857059E-4	0.0	1.2352753632605143E-4	6.176376816302571E-5
24-25	1.2352753632605143E-5	2.2234956538689257E-4	0.0	1.3588028995865657E-4	7.411652179563085E-5
26-27	1.2352753632605143E-5	2.2234956538689257E-4	0.0	1.8529130448907715E-4	7.411652179563085E-5
28-29	1.2352753632605143E-5	2.347023190194977E-4	0.0	4.261700003248774E-4	7.411652179563085E-5
30-31	1.2352753632605143E-5	3.211715944477337E-4	0.0	0.0013340973923213553	7.411652179563085E-5
32-33	1.2352753632605143E-5	3.5205347852924657E-4	0.0	0.0031499521763143114	8.646927542823599E-5
34-35	1.2352753632605143E-5	3.829353626107594E-4	0.0	0.005114040003898529	9.882202906084115E-5
36-37	1.2352753632605143E-5	4.138172466922723E-4	0.0	0.00838134333972259	9.882202906084115E-5
38-39	1.2352753632605143E-5	4.261700003248774E-4	0.0	0.01359420537268196	9.882202906084115E-5
40-41	1.2352753632605143E-5	4.694046380389954E-4	0.0	0.019128239000089066	9.882202906084115E-5
42-43	1.2352753632605143E-5	4.694046380389954E-4	0.0	0.024161986105375656	9.882202906084115E-5
44-45	1.2352753632605143E-5	5.249920293857186E-4	0.0	0.030344539298494535	9.882202906084115E-5
46-47	1.2352753632605143E-5	5.62050290283534E-4	0.0	0.037157082926876264	9.882202906084115E-5
48-49	1.2352753632605143E-5	5.682266670998365E-4	0.0	0.04409933046840036	9.882202906084115E-5
50-51	2.4705507265210287E-5	5.805794207324417E-4	0.0	0.05023247264698881	9.882202906084115E-5
52-53	2.4705507265210287E-5	5.805794207324417E-4	0.0	0.05679796120271845	9.882202906084115E-5
54-55	2.4705507265210287E-5	5.929321743650469E-4	0.0	0.06361668120791648	9.882202906084115E-5
56-57	2.4705507265210287E-5	6.05284927997652E-4	0.0	0.07009570048821788	9.882202906084115E-5
58-59	2.4705507265210287E-5	6.176376816302571E-4	0.0	0.0761423733913781	9.882202906084115E-5
60-61	2.4705507265210287E-5	6.485195657117699E-4	0.0	0.08227551556996655	9.882202906084115E-5
62-63	2.4705507265210287E-5	6.546959425280726E-4	0.0	0.08754396499427264	9.882202906084115E-5
64-65	2.4705507265210287E-5	6.794014497932829E-4	0.0	0.09357210876698396	9.882202906084115E-5
66-67	2.4705507265210287E-5	6.91754203425888E-4	0.0	0.09900732036533022	9.882202906084115E-5
68-69	2.4705507265210287E-5	7.473415947726112E-4	0.0	0.10434988631143194	9.882202906084115E-5
70-71	2.4705507265210287E-5	7.535179715889137E-4	0.0	0.10919834211222945	1.1117478269344629E-4
72-73	2.4705507265210287E-5	7.535179715889137E-4	0.0	0.11321298704282613	1.1117478269344629E-4
74-75	2.4705507265210287E-5	7.658707252215188E-4	0.0	0.11581941805930582	1.1117478269344629E-4
76-77	2.4705507265210287E-5	7.967526093030317E-4	0.0	0.11846290733668331	1.1117478269344629E-4
78-79	2.4705507265210287E-5	8.029289861193343E-4	0.0	0.12024170385977846	1.1117478269344629E-4
80-81	2.4705507265210287E-5	8.029289861193343E-4	0.0	0.12087787067185762	1.1735115950974886E-4
82-83	2.4705507265210287E-5	8.029289861193343E-4	0.0	0.12126080603446839	1.2352753632605143E-4
84-85	2.4705507265210287E-5	8.091053629356369E-4	0.0	0.12148315559985527	1.2352753632605143E-4
86-87	2.4705507265210287E-5	8.276344933845445E-4	0.0	0.12158815400573242	1.29703913142354E-4
88	2.4705507265210287E-5	9.264565224453857E-4	0.0	0.12160050675936503	1.3588028995865657E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8665	0.0	46.63919	1
GTATCAA	15185	0.0	33.515614	1
TCAACGC	17530	0.0	29.94143	4
ATCAACG	17915	0.0	29.273903	3
CAACGCA	18160	0.0	28.950212	5
AACGCAG	18865	0.0	27.913006	6
TATCAAC	19125	0.0	27.69344	2
ACGCAGA	22795	0.0	23.157389	7
CGCAGAG	23400	0.0	22.540234	8
TGGTATC	4745	0.0	19.542595	2
GAGTACT	14960	0.0	19.329248	12-13
GCAGAGT	28085	0.0	18.841604	9
GTGGTAT	5090	0.0	18.726406	1
TACATGG	21935	0.0	18.207626	2
GTACATG	21610	0.0	18.102247	1
CAGAGTA	27255	0.0	17.532398	10-11
ACATGGG	22150	0.0	17.368208	3
GTACTTT	16935	0.0	17.138903	14-15
AGAGTAC	24850	0.0	16.495804	10-11
CATGGGG	16510	0.0	15.072754	4
>>END_MODULE