FastQCFastQC Report
Fri 27 May 2016
SRR2139781_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139781_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6656414
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84750.050.574011
GTATCAA158750.038.7099151
TCAACGC179850.033.4249574
ATCAACG180850.033.1211663
CAACGCA184600.032.518275
AACGCAG189800.031.627126
TATCAAC192250.031.516942
ACGCAGA215750.027.8031257
CGCAGAG219550.027.3219038
TGGTATC43100.023.164262
GTGGTAT45350.022.8690171
GCAGAGT262800.022.8581819
GTACATG201050.022.2392021
TACATGG198750.021.9985622
GAGTACT146750.021.2002912-13
ACATGGG201750.021.0730933
CAGAGTA257600.019.86175510-11
CATGGGG145300.019.3685474
AGAGTAC236850.018.6949210-11
GTACTTT165800.018.53140614-15
Produced by FastQC (version 0.11.5)