##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139781_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6656414
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.68346485059373	32.0	18.0	33.0	18.0	33.0
2	29.618677864688102	32.0	28.0	33.0	18.0	33.0
3	31.3749152321355	33.0	32.0	33.0	27.0	33.0
4	31.81640024193207	33.0	32.0	33.0	29.0	33.0
5	32.44112415483772	33.0	33.0	33.0	32.0	34.0
6	36.07088922053226	38.0	36.0	38.0	33.0	38.0
7	36.75955251581407	38.0	37.0	38.0	34.0	38.0
8	36.67009774331945	38.0	37.0	38.0	34.0	38.0
9	36.970410644530226	38.0	38.0	38.0	35.0	38.0
10-11	37.10556284509948	38.0	38.0	38.0	36.0	38.0
12-13	37.140361762354324	38.0	38.0	38.0	36.0	38.0
14-15	37.12780274784591	38.0	38.0	38.0	36.0	38.0
16-17	37.076255398176855	38.0	38.0	38.0	36.0	38.0
18-19	37.11342901748599	38.0	38.0	38.0	36.0	38.0
20-21	37.14625385050358	38.0	38.0	38.0	36.0	38.0
22-23	37.156916773622704	38.0	38.0	38.0	36.0	38.0
24-25	37.161530305956134	38.0	38.0	38.0	36.0	38.0
26-27	37.132512526892924	38.0	38.0	38.0	36.0	38.0
28-29	37.10838973508264	38.0	38.0	38.0	36.0	38.0
30-31	37.10963870148869	38.0	38.0	38.0	36.0	38.0
32-33	37.10293751495363	38.0	38.0	38.0	36.0	38.0
34-35	37.080515303793774	38.0	38.0	38.0	36.0	38.0
36-37	37.06630250386453	38.0	38.0	38.0	36.0	38.0
38-39	37.04862922407621	38.0	38.0	38.0	36.0	38.0
40-41	37.02800967586329	38.0	38.0	38.0	36.0	38.0
42-43	37.003105708979675	38.0	38.0	38.0	36.0	38.0
44-45	36.95386366499571	38.0	38.0	38.0	36.0	38.0
46-47	36.93129554754497	38.0	38.0	38.0	36.0	38.0
48-49	36.89717918148135	38.0	38.0	38.0	36.0	38.0
50-51	36.85489565515478	38.0	38.0	38.0	35.0	38.0
52-53	36.826779224968774	38.0	38.0	38.0	35.0	38.0
54-55	36.79390218632042	38.0	38.0	38.0	35.0	38.0
56-57	36.79244192567121	38.0	38.0	38.0	35.0	38.0
58-59	36.773366012271964	38.0	38.0	38.0	35.0	38.0
60-61	36.7804396732315	38.0	38.0	38.0	35.0	38.0
62-63	36.76438014524985	38.0	38.0	38.0	35.0	38.0
64-65	36.751016270030455	38.0	38.0	38.0	35.0	38.0
66-67	36.73770177685543	38.0	38.0	38.0	35.0	38.0
68-69	36.7394544462815	38.0	38.0	38.0	35.0	38.0
70-71	36.71402232601952	38.0	38.0	38.0	34.5	38.0
72-73	36.69454029384154	38.0	38.0	38.0	34.0	38.0
74-75	36.705132210081665	38.0	38.0	38.0	34.0	38.0
76-77	36.67740052534646	38.0	38.0	38.0	34.0	38.0
78-79	36.64518879593483	38.0	38.0	38.0	34.0	38.0
80-81	36.63478335094892	38.0	38.0	38.0	34.0	38.0
82-83	36.57166993479203	38.0	38.0	38.0	34.0	38.0
84-85	36.56567911800161	38.0	38.0	38.0	34.0	38.0
86-87	36.54639750371044	38.0	38.0	38.0	34.0	38.0
88-89	36.51726967377991	38.0	38.0	38.0	34.0	38.0
90-91	36.492020381107714	38.0	38.0	38.0	34.0	38.0
92-93	36.49138535209346	38.0	38.0	38.0	34.0	38.0
94-95	36.43037143883339	38.0	38.0	38.0	34.0	38.0
96-97	36.394348148766454	38.0	38.0	38.0	34.0	38.0
98-99	36.42547030490408	38.0	37.5	38.0	34.0	38.0
100	34.712776597214365	38.0	34.0	38.0	27.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	28.0
20	89.0
21	245.0
22	789.0
23	2305.0
24	5300.0
25	10184.0
26	17361.0
27	27628.0
28	41175.0
29	57692.0
30	78575.0
31	102773.0
32	134481.0
33	179651.0
34	259467.0
35	440851.0
36	1158151.0
37	4139668.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.84187732313525	18.104973638959358	14.26590954228508	22.787239495620316
2	14.496115867006662	19.560610037314177	43.356485240865524	22.586788854813637
3	18.28828555435404	25.64607610043486	29.95166766970925	26.113970675501854
4	12.420486466136271	18.029046871183194	38.421228006551274	31.12923865612926
5	13.803993893269226	37.0039971383338	35.457648752407884	13.734360215989094
6	30.49690906913923	37.39342038627734	18.617245424672674	13.49242511991075
7	27.33321575250578	32.543047953447605	22.303856100296645	17.819880193749967
8	24.604118674108914	36.961538149520145	20.580165236116624	17.854177940254317
9	25.477471803887198	17.460737868768376	21.120320941576047	35.94146938576837
10-11	24.491588113359537	26.909556707260094	28.324627945317104	20.274227234063265
12-13	25.222672447957716	24.21451700570307	28.69156726129114	21.871243285048074
14-15	22.2067467558358	25.75370161771789	26.445462977513117	25.594088648933194
16-17	21.81667185965296	28.97170308217007	27.11948956299894	22.092135495178034
18-19	21.770310560611165	28.01779606857386	29.00002914482182	21.211864225993153
20-21	22.59331924699352	27.08388653478069	29.01593453029413	21.306859687931663
22-23	22.337905137155243	26.990048489921055	28.86939256178388	21.80265381113983
24-25	22.049179139443563	27.07182992815888	28.96806112208453	21.910929810313025
26-27	22.009337586150885	27.235835883155605	28.924037860634627	21.830788670058883
28-29	21.925512460306976	27.291448821906055	28.90969627311296	21.87334244467401
30-31	22.238172503875564	27.10564589577466	28.80216217826192	21.854019422087852
32-33	21.964470060676806	27.257387151833967	28.856613420658384	21.921529366830843
34-35	22.160326063356216	27.26427578813132	28.79895279583589	21.776445352676575
36-37	22.063759425620255	27.2285843882664	28.81238795263724	21.895268233476102
38-39	21.921236557685578	27.337957543083103	28.902641760839664	21.838164138391654
40-41	22.242268718751433	27.19045272765579	28.718610416815636	21.848668136777142
42-43	22.097280381103328	27.261187612383853	28.606528500980875	22.03500350553194
44-45	22.17354497568161	27.356927504416635	28.46634988705728	22.003177632844476
46-47	22.213522326390425	27.38893375860164	28.27093354797716	22.126610367030768
48-49	22.095688434188183	27.482649360191335	28.264151399137695	22.15751080648279
50-51	22.12353287246136	27.627066083740694	28.14115474731298	22.10824629648497
52-53	22.332135070494058	27.507877616994826	28.031164445555135	22.12882286695598
54-55	22.266275396395883	27.399217238972977	28.108661472835884	22.225845891795252
56-57	22.168635588713002	27.61147399644469	28.08517102280781	22.134719392034498
58-59	22.193503805722614	27.604811981198857	28.084047484828634	22.117636728249895
60-61	22.273592723911495	27.507417579378295	28.143870575396768	22.07511912131344
62-63	22.238310229054985	27.566365246710568	28.104942796985956	22.090381727248495
64-65	22.22986419907197	27.526869154275445	28.06879187583714	22.174474770815447
66-67	22.269982440681837	27.53405894944142	27.93758095564106	22.258377654235684
68-69	22.218816788709542	27.55183650008811	28.014613600899807	22.21473311030254
70-71	22.2349018356256	27.39084603007554	28.027968652830783	22.346283481468085
72-73	22.294529314342036	27.44027544740122	27.957671812641223	22.30752342561552
74-75	22.137984839195237	27.569172155687315	27.923186655273174	22.369656349844274
76-77	22.31374294977579	27.471326493229043	27.892022106235654	22.322908450759517
78-79	22.25360133636574	27.50380541483762	27.832209088152197	22.41038416064445
80-81	22.25346975644441	27.53330287933975	27.847598873292075	22.36562849092377
82-83	22.309202796832544	27.54316866588716	27.881743771038554	22.265884766241744
84-85	22.38331729841853	27.48195374682408	27.860178957514993	22.274549997242403
86-87	22.2308420812804	27.569471352666895	27.893895697162602	22.3057908688901
88-89	22.33453159532555	27.51701160638528	27.842375696310455	22.306081101978716
90-91	22.369013569796923	27.564201599486342	27.796339574048645	22.270445256668086
92-93	22.321280413849028	27.54384154186741	27.84510275422259	22.289775290060977
94-95	22.26455585652999	27.61209777004548	27.82334974461994	22.29999662880459
96-97	22.392783376527497	27.719571526638525	27.8407109394899	22.04693415734408
98-99	23.44297840516368	29.615847867159534	28.081326058428868	18.859847669247923
100	22.305875062760123	30.489236108161567	22.037755774042996	25.16713305503532
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	680.0
1	667.0
2	989.5
3	1465.0
4	1983.5
5	3033.5
6	4040.0
7	5077.5
8	6432.5
9	7360.0
10	7418.0
11	7439.0
12	8376.5
13	10281.5
14	13909.0
15	18455.0
16	22295.5
17	25069.5
18	25966.5
19	24750.0
20	22435.5
21	20270.5
22	19752.0
23	21954.5
24	27001.5
25	34793.0
26	45222.0
27	57093.5
28	69904.5
29	84996.5
30	101043.0
31	117256.5
32	135133.5
33	152661.0
34	170008.5
35	186187.5
36	202665.5
37	219213.5
38	231523.0
39	241643.0
40	252354.5
41	261001.0
42	267330.0
43	273472.5
44	276867.5
45	280357.0
46	282219.0
47	280728.5
48	278504.5
49	273784.5
50	267946.5
51	259030.5
52	247079.5
53	235378.0
54	221841.0
55	206163.0
56	188968.5
57	170885.0
58	152911.5
59	133115.5
60	111704.0
61	91381.0
62	72643.0
63	56566.0
64	43303.5
65	32465.5
66	24402.5
67	18946.0
68	14719.5
69	10880.0
70	7869.0
71	5677.0
72	4095.0
73	2803.5
74	1811.0
75	1200.5
76	767.5
77	430.5
78	254.5
79	172.5
80	120.0
81	76.0
82	50.0
83	37.0
84	27.5
85	17.0
86	10.0
87	7.5
88	5.0
89	2.5
90	1.5
91	1.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003470337031320468
3	0.0
4	0.0
5	0.002283511812816931
6	7.511552015845169E-5
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.506955163186291E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	1.5659810044938174E-5
48-49	4.578244607873744E-4
50-51	0.0016940611465405839
52-53	0.003924637351494526
54-55	0.005613997502577721
56-57	0.006330947646963929
58-59	0.0077155336790416616
60-61	0.007438078819364729
62-63	0.005598690056268915
64-65	0.003353265984662664
66-67	0.0010987659590473023
68-69	5.882508403887911E-4
70-71	3.0100732531426885E-4
72-73	5.898493689522549E-4
74-75	8.746280972002193E-6
76-77	2.911074609960109E-4
78-79	2.2252537100765009E-4
80-81	8.983470862786017E-5
82-83	4.5330693757256315E-5
84-85	9.146249722068337E-5
86-87	1.2919006044895205E-4
88-89	2.794743162637563E-5
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	54.0
20-21	162.0
22-23	381.0
24-25	557.0
26-27	1301.0
28-29	3637.0
30-31	11213.0
32-33	14077.0
34-35	20107.0
36-37	32800.0
38-39	38757.0
40-41	38309.0
42-43	43962.0
44-45	51999.0
46-47	52388.0
48-49	48581.0
50-51	44961.0
52-53	43093.0
54-55	45973.0
56-57	47590.0
58-59	47820.0
60-61	47003.0
62-63	45763.0
64-65	47711.0
66-67	50562.0
68-69	51029.0
70-71	50455.0
72-73	47706.0
74-75	48040.0
76-77	50255.0
78-79	51487.0
80-81	51196.0
82-83	48831.0
84-85	47907.0
86-87	50530.0
88-89	52689.0
90-91	53386.0
92-93	53270.0
94-95	65223.0
96-97	209317.0
98-99	472950.0
100-101	4473382.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.15473177192861
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.68780666679905	79.0387146502939
2	6.805582307865794	11.862774011876523
3	1.484203479142413	3.880660683588607
4	0.5019472432868776	1.7498830940930719
5	0.21944752241214335	0.9562944976922323
6	0.10599810081335304	0.5542941626832978
7	0.06036747088836587	0.3682917513117665
8	0.03416429424878508	0.23820639211440853
9	0.02088159246123386	0.16379366309366075
>10	0.07750497572668828	1.0090562674009222
>50	0.0015952957031857052	0.09584511667452197
>100	5.010506520953386E-4	0.0821857091769531
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5023104031690336E-5
2	0.0	3.0046208063380672E-5	0.0	0.0	1.5023104031690336E-5
3	0.0	3.0046208063380672E-5	0.0	0.0	1.5023104031690336E-5
4	0.0	6.0092416126761345E-5	0.0	1.5023104031690336E-5	1.5023104031690336E-5
5	0.0	6.0092416126761345E-5	0.0	1.5023104031690336E-5	1.5023104031690336E-5
6	0.0	6.0092416126761345E-5	0.0	1.5023104031690336E-5	1.5023104031690336E-5
7	0.0	6.0092416126761345E-5	0.0	1.5023104031690336E-5	1.5023104031690336E-5
8	0.0	6.0092416126761345E-5	0.0	1.5023104031690336E-5	1.5023104031690336E-5
9	0.0	2.2534656047535506E-4	0.0	3.0046208063380672E-5	1.5023104031690336E-5
10-11	0.0	2.4036966450704538E-4	0.0	3.755776007922584E-5	1.5023104031690336E-5
12-13	0.0	2.4036966450704538E-4	0.0	4.506931209507101E-5	2.2534656047535505E-5
14-15	0.0	3.0046208063380674E-4	0.0	5.258086411091618E-5	6.760396814260651E-5
16-17	0.0	3.0046208063380674E-4	0.0	6.0092416126761345E-5	9.013862419014202E-5
18-19	1.5023104031690336E-5	3.079736326496519E-4	0.0	8.262707217429686E-5	1.201848322535227E-4
20-21	1.5023104031690336E-5	3.154851846654971E-4	0.0	1.5023104031690337E-4	1.5023104031690337E-4
22-23	1.5023104031690336E-5	3.605544967605681E-4	0.0	1.5023104031690337E-4	2.0281190442781954E-4
24-25	1.5023104031690336E-5	3.906007048239487E-4	0.0	1.7276569636443887E-4	2.854389766021164E-4
26-27	1.5023104031690336E-5	3.906007048239487E-4	0.0	1.9530035241197436E-4	3.0046208063380674E-4
28-29	3.0046208063380672E-5	3.981122568397939E-4	0.0	3.830891528081036E-4	3.079736326496519E-4
30-31	3.0046208063380672E-5	4.657162249824004E-4	0.0	0.0010816634902817042	3.154851846654971E-4
32-33	3.0046208063380672E-5	4.657162249824004E-4	0.0	0.0027867857978785575	3.305082886971874E-4
34-35	3.0046208063380672E-5	4.95762433045781E-4	0.0	0.0046496506978081584	3.605544967605681E-4
36-37	3.0046208063380672E-5	5.258086411091617E-4	0.0	0.008014826000906795	3.605544967605681E-4
38-39	3.0046208063380672E-5	5.408317451408521E-4	0.0	0.0136034207006956	3.7557760079225843E-4
40-41	3.0046208063380672E-5	5.708779532042328E-4	0.0	0.018853995559771373	3.830891528081036E-4
42-43	3.0046208063380672E-5	5.783895052200779E-4	0.0	0.023826642994260874	4.1313536087148425E-4
44-45	3.0046208063380672E-5	6.384819213468393E-4	0.0	0.029542934078319046	4.3567001691901977E-4
46-47	3.0046208063380672E-5	6.910627854577555E-4	0.0	0.03614558830024695	4.506931209507101E-4
48-49	3.0046208063380672E-5	6.985743374736007E-4	0.0	0.042845892698380836	4.957624330457812E-4
50-51	3.0046208063380672E-5	8.337822737588137E-4	0.0	0.049741497448926704	4.957624330457812E-4
52-53	3.0046208063380672E-5	8.412938257746588E-4	0.0	0.056299082358759536	5.107855370774714E-4
54-55	3.0046208063380672E-5	8.48805377790504E-4	0.0	0.06321722176535294	5.408317451408521E-4
56-57	3.0046208063380672E-5	8.713400338380395E-4	0.0	0.06904618612964879	5.558548491725424E-4
58-59	3.0046208063380672E-5	8.788515858538847E-4	0.0	0.07493524291007139	5.633664011883877E-4
60-61	4.506931209507101E-5	8.938746898855751E-4	0.0	0.08054637226590775	5.708779532042328E-4
62-63	4.506931209507101E-5	9.164093459331106E-4	0.0	0.08611243230964902	5.708779532042328E-4
64-65	4.506931209507101E-5	9.915248660915623E-4	0.0	0.09168600390540613	5.783895052200779E-4
66-67	4.506931209507101E-5	0.0010065479701232526	0.0	0.09726708705317909	5.859010572359231E-4
68-69	4.506931209507101E-5	0.0010140595221390977	0.0	0.10296835503320556	5.934126092517684E-4
70-71	5.258086411091618E-5	0.0010215710741549428	0.0	0.10777574832334648	6.459934733626844E-4
72-73	6.0092416126761345E-5	0.0010290826261707881	0.0	0.1115540589873166	7.211089935211362E-4
74-75	6.0092416126761345E-5	0.0010591288342341686	0.0	0.11434084478519516	7.211089935211362E-4
76-77	6.0092416126761345E-5	0.0010816634902817042	0.0	0.11725532696734307	7.286205455369814E-4
78-79	6.0092416126761345E-5	0.0010966865943133946	0.0	0.11944118860395403	7.361320975528265E-4
80-81	6.0092416126761345E-5	0.0011041981463292398	0.0	0.1200045550051424	7.661783056162072E-4
82-83	6.0092416126761345E-5	0.00111922125036093	0.0	0.12035759794988712	7.962245136795878E-4
84-85	6.0092416126761345E-5	0.0011417559064084656	0.0	0.12061299071842586	7.962245136795878E-4
86-87	6.0092416126761345E-5	0.0011567790104401558	0.0	0.12074068710269524	8.112476177112781E-4
88	6.0092416126761345E-5	0.0011868252185035365	0.0	0.12074068710269524	8.112476177112781E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8475	0.0	50.57401	1
GTATCAA	15875	0.0	38.709915	1
TCAACGC	17985	0.0	33.424957	4
ATCAACG	18085	0.0	33.121166	3
CAACGCA	18460	0.0	32.51827	5
AACGCAG	18980	0.0	31.62712	6
TATCAAC	19225	0.0	31.51694	2
ACGCAGA	21575	0.0	27.803125	7
CGCAGAG	21955	0.0	27.321903	8
TGGTATC	4310	0.0	23.16426	2
GTGGTAT	4535	0.0	22.869017	1
GCAGAGT	26280	0.0	22.858181	9
GTACATG	20105	0.0	22.239202	1
TACATGG	19875	0.0	21.998562	2
GAGTACT	14675	0.0	21.20029	12-13
ACATGGG	20175	0.0	21.073093	3
CAGAGTA	25760	0.0	19.861755	10-11
CATGGGG	14530	0.0	19.368547	4
AGAGTAC	23685	0.0	18.69492	10-11
GTACTTT	16580	0.0	18.531406	14-15
>>END_MODULE