FastQCFastQC Report
Fri 27 May 2016
SRR2139780_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139780_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6710279
Sequences flagged as poor quality0
Sequence length19-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82800.049.5473181
GTATCAA148750.035.5178761
TCAACGC173950.030.1710914
ATCAACG176200.029.785823
CAACGCA179900.029.1732165
TATCAAC184750.028.7135622
AACGCAG186850.028.1564436
TGGTATC40500.024.3665432
GTGGTAT41350.024.282531
ACGCAGA220100.023.824617
CGCAGAG224800.023.3264968
TACATGG207650.020.3587252
GTACATG208900.020.2781621
GCAGAGT264500.019.7927369
ACATGGG213300.019.1911473
GAGTACT143950.019.12689412-13
CAGAGTA256850.018.13432310-11
GTACTTT157050.017.43543814-15
CATGGGG163150.017.40474
AGAGTAC239050.017.34871110-11
Produced by FastQC (version 0.11.5)