##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139780_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6710279
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.24075839469566	32.0	25.0	33.0	18.0	33.0
2	28.413161956455163	31.0	25.0	33.0	18.0	33.0
3	31.192825663433666	33.0	31.0	33.0	27.0	33.0
4	31.489661607214842	33.0	31.0	33.0	29.0	34.0
5	32.55924321477542	33.0	33.0	33.0	32.0	34.0
6	36.50443431636747	38.0	37.0	38.0	34.0	38.0
7	36.74949342642832	38.0	37.0	38.0	34.0	38.0
8	36.99212566273325	38.0	38.0	38.0	35.0	38.0
9	37.07390035496289	38.0	38.0	38.0	36.0	38.0
10-11	37.14629779179077	38.0	38.0	38.0	36.0	38.0
12-13	37.175262757330955	38.0	38.0	38.0	36.0	38.0
14-15	37.180173357918505	38.0	38.0	38.0	36.0	38.0
16-17	37.15048278916569	38.0	38.0	38.0	36.0	38.0
18-19	37.17191632419457	38.0	38.0	38.0	36.0	38.0
20-21	37.19306306203715	38.0	38.0	38.0	36.0	38.0
22-23	37.2019203863096	38.0	38.0	38.0	36.0	38.0
24-25	37.206778418433686	38.0	38.0	38.0	36.0	38.0
26-27	37.177599154750226	38.0	38.0	38.0	36.0	38.0
28-29	37.15019908671	38.0	38.0	38.0	36.0	38.0
30-31	37.151185575073285	38.0	38.0	38.0	36.0	38.0
32-33	37.14530326750496	38.0	38.0	38.0	36.0	38.0
34-35	37.12825149378591	38.0	38.0	38.0	36.0	38.0
36-37	37.11276130051671	38.0	38.0	38.0	36.0	38.0
38-39	37.09925359726853	38.0	38.0	38.0	36.0	38.0
40-41	37.08165156205273	38.0	38.0	38.0	36.0	38.0
42-43	37.06498278409033	38.0	38.0	38.0	36.0	38.0
44-45	37.0217158209081	38.0	38.0	38.0	36.0	38.0
46-47	37.005058409753055	38.0	38.0	38.0	36.0	38.0
48-49	36.977872002736866	38.0	38.0	38.0	36.0	38.0
50-51	36.94205730896503	38.0	38.0	38.0	36.0	38.0
52-53	36.919632396885945	38.0	38.0	38.0	36.0	38.0
54-55	36.89652174506418	38.0	38.0	38.0	35.5	38.0
56-57	36.901600664428955	38.0	38.0	38.0	36.0	38.0
58-59	36.89050026120148	38.0	38.0	38.0	35.0	38.0
60-61	36.903446779515534	38.0	38.0	38.0	35.5	38.0
62-63	36.89385233369429	38.0	38.0	38.0	35.0	38.0
64-65	36.88703681185875	38.0	38.0	38.0	35.0	38.0
66-67	36.88028092460331	38.0	38.0	38.0	35.0	38.0
68-69	36.88683522820781	38.0	38.0	38.0	35.0	38.0
70-71	36.866503039198534	38.0	38.0	38.0	35.0	38.0
72-73	36.850873129818375	38.0	38.0	38.0	35.0	38.0
74-75	36.86578485633716	38.0	38.0	38.0	35.0	38.0
76-77	36.84719250372844	38.0	38.0	38.0	35.0	38.0
78-79	36.823730850705104	38.0	38.0	38.0	35.0	38.0
80-81	36.81451651432459	38.0	38.0	38.0	35.0	38.0
82-83	36.75977950290725	38.0	38.0	38.0	35.0	38.0
84-85	36.76138269090053	38.0	38.0	38.0	35.0	38.0
86-87	36.7524756335588	38.0	38.0	38.0	35.0	38.0
88-89	36.726953901487896	38.0	38.0	38.0	35.0	38.0
90-91	36.70797360348932	38.0	38.0	38.0	35.0	38.0
92-93	36.70605388490799	38.0	38.0	38.0	34.5	38.0
94-95	36.65454433054444	38.0	38.0	38.0	34.0	38.0
96-97	36.62376702554593	38.0	38.0	38.0	34.0	38.0
98-99	36.65339417121237	38.0	38.0	38.0	34.0	38.0
100	35.104490206378394	38.0	35.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	7.0
18	15.0
19	32.0
20	59.0
21	185.0
22	550.0
23	1663.0
24	4062.0
25	8343.0
26	14824.0
27	24016.0
28	36806.0
29	51924.0
30	71512.0
31	94650.0
32	123724.0
33	166801.0
34	242010.0
35	422728.0
36	1141625.0
37	4304743.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67599871778803	20.88485441514429	14.314486774692975	24.124660092374697
2	15.466472885430818	19.84106640087321	41.400873207816666	23.29158750587931
3	18.59699127264306	25.185450560252416	29.66328225696726	26.55427591013727
4	13.070052675902149	17.45083922740023	37.9053240558254	31.573784040872223
5	13.935311977512052	36.89067631503217	35.226568732458304	13.947442974997479
6	31.220403584236227	37.02829035842362	18.06581014897757	13.685495908362583
7	27.637747998257602	32.22035924288692	21.9273893082538	18.214503450601683
8	24.96626444295386	36.806293747249555	20.133499665215112	18.093942144581472
9	25.517180433183178	17.120241945230593	20.790134061489844	36.57244356009638
10-11	24.889061989821883	26.603402034401253	27.97265210582153	20.534883869955333
12-13	25.546150912652067	23.865714078356504	28.377203093939908	22.21093191505152
14-15	22.542535116647162	25.400836537497174	26.021615494676155	26.035012851179513
16-17	22.22327864459883	28.606440954243485	26.681088819108712	22.489191582048974
18-19	22.2007833057314	27.644990618124822	28.53083306968309	21.623393006460685
20-21	23.018693113176905	26.66259425857521	28.55402861778232	21.76468401046556
22-23	22.766841867754476	26.596996067364493	28.376227495542476	22.259934569338558
24-25	22.45943708553752	26.719140190139022	28.499563205013303	22.321859519310152
26-27	22.42441171017071	26.869033856661723	28.427378125378215	22.27917630778935
28-29	22.353491133329097	26.93762842010067	28.41544914496924	22.29343130160099
30-31	22.64529609779253	26.754127575125473	28.340075202873415	22.260501124208577
32-33	22.386431094645886	26.899954027826016	28.34528271093546	22.368332166592637
34-35	22.518979405001417	26.90208957691999	28.3776106230745	22.201320395004092
36-37	22.51897598548093	26.819247534198325	28.316669222823016	22.345107257497723
38-39	22.373934884952014	26.97455507718562	28.3740666221701	22.27744341569226
40-41	22.66585441886534	26.841938153124463	28.21759802532004	22.274609402690157
42-43	22.5457756758781	26.868628892594725	28.111332068728856	22.47426336279831
44-45	22.599512655158193	26.970539664147335	28.0105457471074	22.419401933587064
46-47	22.626489665808908	27.039960020352883	27.747811402127713	22.5857389117105
48-49	22.527499250521586	27.137444410752014	27.775279081990984	22.559777256735416
50-51	22.56112062320565	27.245025647206454	27.669009044564262	22.52484468502363
52-53	22.760343376877756	27.07937447005273	27.565103444676105	22.595178708393412
54-55	22.63319736379115	27.054099315945262	27.646427534411423	22.666275785852164
56-57	22.62631401770992	27.18711222680789	27.618919392427937	22.567654363054253
58-59	22.651836065119067	27.196806576691117	27.599244806871255	22.552112551318558
60-61	22.733700646919235	27.124848343186343	27.67202653549842	22.469424474396
62-63	22.665273805565615	27.176765403688464	27.63903785542207	22.51892293532385
64-65	22.624579775830114	27.191451987280885	27.58400273171117	22.59996550517783
66-67	22.699766070251183	27.192660700839212	27.457583716107703	22.64998951280191
68-69	22.639000959059196	27.125771871437315	27.54196864464297	22.693258524860514
70-71	22.638816139337937	26.999056281056568	27.587712988637	22.77441459096849
72-73	22.70949904243766	27.031029961637447	27.50643079340659	22.753040202518303
74-75	22.552869430810556	27.172052539233384	27.480271406286423	22.794806623669643
76-77	22.735743629144004	27.100420716041484	27.378987991141734	22.784847663672775
78-79	22.676651694287518	27.115622163861055	27.34092939739796	22.866796744453467
80-81	22.70660781122929	27.1327082495906	27.362086759293568	22.798597179886542
82-83	22.734275319579865	27.142492494927406	27.415969482820827	22.7072627026719
84-85	22.84961217585193	27.09501535744407	27.36055920633792	22.694813260366082
86-87	22.677710240454505	27.136148899562144	27.425856306293	22.76028455369035
88-89	22.72974272553315	27.13883220874052	27.35971536788761	22.77170969783872
90-91	22.79576047066084	27.165203259372955	27.35329259442249	22.685743675543716
92-93	22.74903858780273	27.14487778340556	27.392978408088993	22.713105220702722
94-95	22.699370805746756	27.18455590845103	27.391276948291782	22.72479633751043
96-97	22.800625190489534	27.333871963327493	27.34995774595854	22.515545100224433
98-99	23.90402476135763	29.227227502258412	27.55061133244246	19.3181364039415
100	22.792662934398596	30.036947205461157	21.498110977404693	25.672278882735554
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	656.0
1	658.0
2	987.5
3	1456.0
4	1913.0
5	2798.0
6	3619.0
7	4602.0
8	5806.5
9	6537.5
10	6837.0
11	7185.5
12	8238.0
13	10310.0
14	14156.5
15	18875.5
16	22297.0
17	24059.0
18	24068.5
19	22500.5
20	20395.5
21	18193.5
22	17319.5
23	19011.5
24	23057.5
25	29775.5
26	38820.5
27	49871.0
28	61990.0
29	75732.0
30	89839.5
31	104977.0
32	122030.0
33	137492.0
34	153035.5
35	168634.0
36	185357.0
37	202428.5
38	216454.5
39	226894.0
40	238360.0
41	250699.5
42	259641.5
43	267614.5
44	273902.0
45	279726.5
46	284295.5
47	285639.0
48	285309.5
49	283552.0
50	280503.5
51	273962.5
52	265113.5
53	256108.5
54	245349.5
55	230608.5
56	213096.0
57	194128.0
58	173005.5
59	150144.5
60	126117.0
61	104009.5
62	83507.0
63	64450.0
64	49068.0
65	37081.0
66	27638.0
67	21087.0
68	16191.0
69	11904.5
70	8688.0
71	6315.0
72	4562.0
73	3153.0
74	2123.5
75	1393.0
76	870.5
77	531.5
78	331.5
79	212.5
80	152.5
81	104.0
82	62.5
83	41.0
84	29.5
85	20.0
86	14.0
87	9.0
88	5.0
89	3.5
90	4.0
91	2.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.004575070574561802
3	0.0
4	0.0
5	0.0029208919629124214
6	1.0431757010401505E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4903118552072614E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.98908714175417E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	7.597727671607975E-6
42-43	0.0
44-45	0.0
46-47	2.3272841013448444E-5
48-49	3.987819167257346E-4
50-51	0.0015597228514285999
52-53	0.003942346865604206
54-55	0.0058229329945754865
56-57	0.006494068750541173
58-59	0.00806903787711804
60-61	0.007690028280671485
62-63	0.005549398705359863
64-65	0.0033846587521509795
66-67	0.0010788149594001789
68-69	4.723160786429887E-4
70-71	2.3820488120935258E-4
72-73	4.718724146217646E-4
74-75	4.324784387874342E-5
76-77	2.703767237388321E-4
78-79	2.199831457713036E-4
80-81	1.1543054171375641E-4
82-83	8.959407790312587E-6
84-85	1.1747714753195876E-4
86-87	1.1849705265926984E-4
88-89	2.7597738310150006E-5
90-91	9.288820069053088E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	48.0
20-21	152.0
22-23	328.0
24-25	469.0
26-27	1172.0
28-29	3368.0
30-31	10637.0
32-33	13737.0
34-35	19136.0
36-37	32299.0
38-39	38649.0
40-41	37916.0
42-43	43205.0
44-45	50791.0
46-47	51680.0
48-49	48073.0
50-51	44810.0
52-53	42804.0
54-55	45730.0
56-57	47814.0
58-59	47515.0
60-61	46027.0
62-63	45065.0
64-65	47469.0
66-67	50474.0
68-69	50917.0
70-71	50271.0
72-73	47414.0
74-75	47281.0
76-77	49965.0
78-79	51051.0
80-81	51021.0
82-83	48275.0
84-85	47191.0
86-87	49446.0
88-89	52005.0
90-91	52423.0
92-93	52725.0
94-95	64204.0
96-97	211368.0
98-99	478881.0
100-101	4536473.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	85.13016923417221
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.49631867562898	76.18836754691702
2	7.407169381135535	12.611471659244936
3	1.7932895062211307	4.579891174514099
4	0.6281759335411501	2.139068941247689
5	0.273416567008831	1.1637999310444085
6	0.13688350154382284	0.6991749391075027
7	0.07979454735347447	0.4755046324115815
8	0.04946297450197535	0.33686331121429647
9	0.030695760789042587	0.23518217796685767
>10	0.10196064279748228	1.3171933447676474
>50	0.002086492871648986	0.11502230024106204
>100	6.907329854206037E-4	0.09772698011375797
>500	2.7641811865184928E-5	0.015977902908531703
>1k	2.7641809606189176E-5	0.024755158300587617
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	1.4902510014859293E-5	0.0	0.0	1.4902510014859293E-5
2	0.0	2.9805020029718586E-5	0.0	0.0	1.4902510014859293E-5
3	0.0	2.9805020029718586E-5	0.0	0.0	1.4902510014859293E-5
4	1.4902510014859293E-5	4.470753004457788E-5	0.0	2.9805020029718586E-5	1.4902510014859293E-5
5	1.4902510014859293E-5	5.961004005943717E-5	0.0	4.470753004457788E-5	1.4902510014859293E-5
6	1.6392761016345223E-4	5.961004005943717E-5	0.0	4.470753004457788E-5	1.4902510014859293E-5
7	1.6392761016345223E-4	5.961004005943717E-5	0.0	4.470753004457788E-5	1.4902510014859293E-5
8	1.6392761016345223E-4	5.961004005943717E-5	0.0	5.961004005943717E-5	1.4902510014859293E-5
9	1.6392761016345223E-4	1.0431757010401505E-4	0.0	5.961004005943717E-5	1.4902510014859293E-5
10-11	1.7137886517088187E-4	1.0431757010401505E-4	0.0	7.451255007429646E-5	1.4902510014859293E-5
12-13	1.7883012017831152E-4	1.0431757010401505E-4	0.0	7.451255007429646E-5	1.4902510014859293E-5
14-15	1.7883012017831152E-4	1.4902510014859293E-4	0.0	8.941506008915576E-5	3.725627503714823E-5
16-17	3.2785522032690445E-4	1.4902510014859293E-4	0.0	8.941506008915576E-5	8.941506008915576E-5
18-19	3.2785522032690445E-4	1.5647635515602258E-4	0.0	8.941506008915576E-5	1.0431757010401505E-4
20-21	3.2785522032690445E-4	1.7883012017831152E-4	0.0	8.941506008915576E-5	1.0431757010401505E-4
22-23	3.2785522032690445E-4	2.0118388520060044E-4	0.0	9.686631509658541E-5	1.3412259013373363E-4
24-25	3.5020898534919337E-4	2.086351402080301E-4	0.0	1.0431757010401505E-4	1.7883012017831152E-4
26-27	3.7256275037148235E-4	2.086351402080301E-4	0.0	1.1922008011887434E-4	2.1608639521545974E-4
28-29	3.8001400537891197E-4	2.1608639521545974E-4	0.0	3.204039653194748E-4	2.235376502228894E-4
30-31	4.172702804160602E-4	2.5334267025260796E-4	0.0	9.91016915988143E-4	2.3098890523031904E-4
32-33	4.172702804160602E-4	2.5334267025260796E-4	0.0	0.0026228417626152356	2.5334267025260796E-4
34-35	4.321727904309195E-4	3.1295271031204515E-4	0.0	0.004694290654680678	2.5334267025260796E-4
36-37	4.470753004457788E-4	3.7256275037148235E-4	0.0	0.008263441803239478	2.5334267025260796E-4
38-39	4.470753004457788E-4	3.7256275037148235E-4	0.0	0.012786353592749274	2.7569643527489693E-4
40-41	4.619778104606381E-4	3.874652603863416E-4	0.0	0.017972427077920308	2.8314769028232656E-4
42-43	4.619778104606381E-4	4.023677704012009E-4	0.0	0.022815742832749578	2.8314769028232656E-4
44-45	4.6942906546806776E-4	4.6942906546806776E-4	0.0	0.028396732833314384	2.8314769028232656E-4
46-47	4.768803204754974E-4	5.215878505200753E-4	0.0	0.03462598201952556	2.905989452897562E-4
48-49	4.768803204754974E-4	5.215878505200753E-4	0.0	0.04119053768107109	3.1295271031204515E-4
50-51	4.917828304903567E-4	6.259054206240903E-4	0.0	0.047337823062200544	3.353064753343341E-4
52-53	4.917828304903567E-4	6.259054206240903E-4	0.0	0.05384276868368663	3.5020898534919337E-4
54-55	4.917828304903567E-4	6.3335667563152E-4	0.0	0.0603477143051727	3.5766024035662305E-4
56-57	5.066853405052159E-4	6.706129506686681E-4	0.0	0.06636832835117587	3.5766024035662305E-4
58-59	5.066853405052159E-4	6.706129506686681E-4	0.0	0.07218775851197842	3.651114953640527E-4
60-61	5.811978905795125E-4	6.929667156909572E-4	0.0	0.0778656148276398	3.949165153937712E-4
62-63	6.11002910609231E-4	7.227717357206757E-4	0.0	0.08302188329278112	4.023677704012009E-4
64-65	6.11002910609231E-4	8.64345580861839E-4	0.0	0.08797696787272183	4.023677704012009E-4
66-67	6.11002910609231E-4	8.64345580861839E-4	0.0	0.09311833382784829	4.023677704012009E-4
68-69	6.11002910609231E-4	8.866993458841279E-4	0.0	0.09835656609807134	4.0981902540863056E-4
70-71	6.3335667563152E-4	9.016018558989872E-4	0.0	0.10296889294767028	4.470753004457788E-4
72-73	6.855154606835275E-4	9.090531109064168E-4	0.0	0.1065678491162588	4.619778104606381E-4
74-75	6.855154606835275E-4	9.314068759287057E-4	0.0	0.10930245970398549	4.619778104606381E-4
76-77	6.855154606835275E-4	9.612118959584244E-4	0.0	0.11168686130636296	4.6942906546806776E-4
78-79	6.855154606835275E-4	9.68663150965854E-4	0.0	0.11366144388333183	4.768803204754974E-4
80-81	6.855154606835275E-4	9.68663150965854E-4	0.0	0.11430225181397077	5.141365955126456E-4
82-83	6.929667156909572E-4	9.68663150965854E-4	0.0	0.1147865833894537	5.961004005943717E-4
84-85	7.004179706983867E-4	9.68663150965854E-4	0.0	0.11498031601964687	5.961004005943717E-4
86-87	7.004179706983867E-4	9.68663150965854E-4	0.0	0.11504737731471373	5.961004005943717E-4
88	7.004179706983867E-4	0.0010133706810104318	0.0	0.1150622798247286	5.961004005943717E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8280	0.0	49.547318	1
GTATCAA	14875	0.0	35.517876	1
TCAACGC	17395	0.0	30.171091	4
ATCAACG	17620	0.0	29.78582	3
CAACGCA	17990	0.0	29.173216	5
TATCAAC	18475	0.0	28.713562	2
AACGCAG	18685	0.0	28.156443	6
TGGTATC	4050	0.0	24.366543	2
GTGGTAT	4135	0.0	24.28253	1
ACGCAGA	22010	0.0	23.82461	7
CGCAGAG	22480	0.0	23.326496	8
TACATGG	20765	0.0	20.358725	2
GTACATG	20890	0.0	20.278162	1
GCAGAGT	26450	0.0	19.792736	9
ACATGGG	21330	0.0	19.191147	3
GAGTACT	14395	0.0	19.126894	12-13
CAGAGTA	25685	0.0	18.134323	10-11
GTACTTT	15705	0.0	17.435438	14-15
CATGGGG	16315	0.0	17.4047	4
AGAGTAC	23905	0.0	17.348711	10-11
>>END_MODULE