FastQCFastQC Report
Fri 27 May 2016
SRR2139779_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139779_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6979736
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88400.049.0904431
GTATCAA159450.036.3875271
TCAACGC191050.031.6586864
ATCAACG192900.031.265353
CAACGCA198450.030.5217675
TATCAAC204250.029.8467622
AACGCAG204250.029.696786
ACGCAGA234600.025.8549337
CGCAGAG237950.025.472758
GTGGTAT45450.023.703571
TGGTATC45350.022.801792
GCAGAGT281400.021.5857129
GAGTACT161300.020.86741412-13
TACATGG204650.019.6827722
CAGAGTA272600.019.30677210-11
GTACTTT176600.018.9252714-15
GTACATG209800.018.9108981
AGAGTAC251700.018.89885310-11
ACATGGG209700.018.444713
AGTACTT178750.017.34177412-13
Produced by FastQC (version 0.11.5)