##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139779_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6979736
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.161680900251813	27.0	18.0	32.0	18.0	33.0
2	31.200452280716636	32.0	31.0	33.0	27.0	33.0
3	31.25770129414637	33.0	31.0	33.0	27.0	33.0
4	31.956560248124	33.0	32.0	33.0	29.0	34.0
5	32.33866696390809	33.0	33.0	33.0	31.0	34.0
6	36.12949186043713	38.0	36.0	38.0	33.0	38.0
7	36.54154498107092	38.0	37.0	38.0	34.0	38.0
8	36.6083823227698	38.0	37.0	38.0	34.0	38.0
9	37.00034170346844	38.0	38.0	38.0	35.0	38.0
10-11	37.047444201327956	38.0	38.0	38.0	36.0	38.0
12-13	37.1034971809822	38.0	38.0	38.0	36.0	38.0
14-15	37.09684392074428	38.0	38.0	38.0	36.0	38.0
16-17	37.06277064060876	38.0	38.0	38.0	36.0	38.0
18-19	37.12002731908485	38.0	38.0	38.0	36.0	38.0
20-21	37.16309637670957	38.0	38.0	38.0	36.0	38.0
22-23	37.18234637810207	38.0	38.0	38.0	36.0	38.0
24-25	37.185754274315244	38.0	38.0	38.0	36.0	38.0
26-27	37.16469674572124	38.0	38.0	38.0	36.0	38.0
28-29	37.14123324693476	38.0	38.0	38.0	36.0	38.0
30-31	37.14019669021478	38.0	38.0	38.0	36.0	38.0
32-33	37.13230590220316	38.0	38.0	38.0	36.0	38.0
34-35	37.11092746051605	38.0	38.0	38.0	36.0	38.0
36-37	37.09458772976373	38.0	38.0	38.0	36.0	38.0
38-39	37.07745097914005	38.0	38.0	38.0	36.0	38.0
40-41	37.05325291212198	38.0	38.0	38.0	36.0	38.0
42-43	37.03546488168151	38.0	38.0	38.0	36.0	38.0
44-45	36.98672921319237	38.0	38.0	38.0	36.0	38.0
46-47	36.96020709861983	38.0	38.0	38.0	36.0	38.0
48-49	36.92829668325042	38.0	38.0	38.0	36.0	38.0
50-51	36.888647343581496	38.0	38.0	38.0	35.5	38.0
52-53	36.859965839116384	38.0	38.0	38.0	35.0	38.0
54-55	36.83041138310495	38.0	38.0	38.0	35.0	38.0
56-57	36.830584518766955	38.0	38.0	38.0	35.0	38.0
58-59	36.813220538774935	38.0	38.0	38.0	35.0	38.0
60-61	36.8219139105628	38.0	38.0	38.0	35.0	38.0
62-63	36.80573589649748	38.0	38.0	38.0	35.0	38.0
64-65	36.792775399171276	38.0	38.0	38.0	35.0	38.0
66-67	36.77902166124268	38.0	38.0	38.0	35.0	38.0
68-69	36.78370094321076	38.0	38.0	38.0	35.0	38.0
70-71	36.758706157337585	38.0	38.0	38.0	35.0	38.0
72-73	36.73917200289843	38.0	38.0	38.0	35.0	38.0
74-75	36.746606510246366	38.0	38.0	38.0	35.0	38.0
76-77	36.7194707324676	38.0	38.0	38.0	35.0	38.0
78-79	36.692219805759464	38.0	38.0	38.0	34.0	38.0
80-81	36.68080180715033	38.0	38.0	38.0	34.0	38.0
82-83	36.62014003082052	38.0	38.0	38.0	34.0	38.0
84-85	36.614061612810424	38.0	38.0	38.0	34.0	38.0
86-87	36.59485547647246	38.0	38.0	38.0	34.0	38.0
88-89	36.566725210612844	38.0	38.0	38.0	34.0	38.0
90-91	36.54007795578408	38.0	38.0	38.0	34.0	38.0
92-93	36.54211862791705	38.0	38.0	38.0	34.0	38.0
94-95	36.484509943497	38.0	38.0	38.0	34.0	38.0
96-97	36.44549604027304	38.0	38.0	38.0	34.0	38.0
98-99	36.4721909527777	38.0	38.0	38.0	34.0	38.0
100	34.78805484165021	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	8.0
18	20.0
19	42.0
20	81.0
21	259.0
22	820.0
23	2232.0
24	5226.0
25	10357.0
26	17275.0
27	27682.0
28	41458.0
29	59090.0
30	79938.0
31	106310.0
32	140185.0
33	187960.0
34	271348.0
35	458794.0
36	1189350.0
37	4381300.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.64847667590866	18.683844202703366	17.98676912708446	23.68090999430351
2	14.48463544554586	20.336109747595316	41.8463427067761	23.33291210008273
3	18.39758695744366	24.536830619381593	30.727494564264322	26.338087858910423
4	12.603427980657148	17.455903776303288	38.14771790795526	31.792950335084306
5	13.651070380294698	36.72228110911383	35.36107693952072	14.265571571070748
6	30.801367789494407	37.33915743720136	18.17486037065336	13.684614402650878
7	27.36182285404491	32.15507004849467	22.059774180570727	18.42333291688969
8	24.560986260798405	37.19368468950688	20.242642415128596	18.00268663456612
9	25.205251316095623	17.429083277648324	20.962827247334285	36.40283815892177
10-11	24.684092636168476	26.82274086011276	28.00586583790562	20.487300665813148
12-13	25.35949067414584	24.037721484021745	28.526838264369886	22.075949577462527
14-15	22.25888629598598	25.678721945930334	26.28489816806825	25.77749359001544
16-17	21.913307322798456	28.85758143287941	26.93281665667584	22.296294587646294
18-19	21.861571841685702	27.86972172013383	28.878979090326624	21.389727347853842
20-21	22.68146838836997	26.86197859674251	28.968463051488985	21.488089963398536
22-23	22.374125654196707	26.833008064778426	28.803668416062735	21.989197864962136
24-25	22.101347468047088	26.939216136087126	28.933723612221396	22.025712783644398
26-27	22.087235025848077	27.0693903579323	28.84157617749372	22.001798438725903
28-29	22.00857311851253	27.123354018508657	28.868157288365516	21.9999155746133
30-31	22.282980814999792	26.955892422052933	28.792518235048544	21.96860852789873
32-33	21.99875544868316	27.118245951239533	28.775605300096263	22.107393299981045
34-35	22.201159977438483	27.139881681610262	28.75045599540017	21.908502345551085
36-37	22.163354085260305	27.047757354826178	28.758112352150643	22.030776207762873
38-39	21.990790216922477	27.21055514384652	28.820354797788365	21.97829984144263
40-41	22.293109795062023	27.036994978650284	28.67151714472294	21.99837808156475
42-43	22.14728129059929	27.143359979849613	28.560007797118736	22.14935093243236
44-45	22.2236564818878	27.22708158756142	28.42098084297869	22.128281087572095
46-47	22.28239433342331	27.24841535039139	28.16917821607081	22.300012100114486
48-49	22.16330901134259	27.352822441463644	28.2147048494908	22.269163697702968
50-51	22.218191297268294	27.483891857035285	28.075069830106337	22.22284701559008
52-53	22.388553130338202	27.374911900948323	27.980540333748845	22.255994634964626
54-55	22.28029299348324	27.317243472725394	28.064418062983442	22.338045470807923
56-57	22.2888215018528	27.434579547762816	28.0259266336781	22.25067231670628
58-59	22.278101516521247	27.42306421499372	28.037669090374056	22.261165178110975
60-61	22.34479289221675	27.384685526198737	28.123837097847293	22.146684483737225
62-63	22.29710521043191	27.42061770445664	28.073195627446307	22.209081457665143
64-65	22.31332834151951	27.378340427137726	28.01271919458342	22.295612036759348
66-67	22.33851100771187	27.38785034645257	27.930211997797088	22.343426648038474
68-69	22.29239070290053	27.386463113855932	27.997024682782833	22.324121500460706
70-71	22.29882755822221	27.220071232950303	28.009771229979215	22.471329978848267
72-73	22.333882461422206	27.291291250874615	27.951623659110126	22.423202628593057
74-75	22.204720150502013	27.44727248186646	27.913460185768784	22.434547181862744
76-77	22.364133665516718	27.341815337407866	27.839731909931636	22.45431908714378
78-79	22.3498224376009	27.363310419334503	27.785460997529604	22.50140614553499
80-81	22.349309661598657	27.41245582652907	27.784880976064564	22.453353535807715
82-83	22.37459773418285	27.441439631071685	27.797545761293783	22.38641687345168
84-85	22.47633397018037	27.375732848708275	27.799564044548227	22.348369136563132
86-87	22.327042957878422	27.40329057917873	27.842536889806475	22.427129573136373
88-89	22.414211509383303	27.443598592341957	27.737285044783455	22.404904853491285
90-91	22.44927425375499	27.4616355563765	27.760508481051037	22.32858170881747
92-93	22.42185243265165	27.42777464177704	27.81437373309647	22.33599919247484
94-95	22.358388410030948	27.475504729123884	27.809011005698085	22.35709585514708
96-97	22.411811838589937	27.64678241669607	27.80876750603144	22.132638238682556
98-99	23.50968653923153	29.515342845918298	28.01737942966136	18.957591185188814
100	22.38742315178427	30.451180948425154	21.96456766244301	25.196828237347567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1164.0
1	1112.0
2	1322.5
3	1761.5
4	2259.5
5	2996.5
6	3790.0
7	5385.0
8	7110.5
9	8008.0
10	8311.5
11	8530.5
12	9587.5
13	12000.5
14	16508.5
15	21822.5
16	25708.0
17	27420.5
18	26966.5
19	24833.0
20	22220.5
21	20135.5
22	19692.0
23	21880.0
24	26807.5
25	34556.0
26	44925.0
27	56691.5
28	69441.0
29	84385.5
30	100309.5
31	116959.0
32	134862.0
33	151826.0
34	168534.0
35	185099.0
36	202382.0
37	219120.0
38	232796.0
39	244604.0
40	257373.0
41	267981.5
42	275483.5
43	284270.5
44	290570.5
45	295581.5
46	298484.0
47	298372.5
48	298446.0
49	294719.0
50	287399.5
51	277148.5
52	265320.0
53	252853.5
54	238117.5
55	220463.5
56	201630.5
57	182543.5
58	161618.5
59	139443.5
60	116809.0
61	95954.0
62	76201.0
63	58600.0
64	44736.5
65	33482.0
66	24798.0
67	18836.0
68	14356.0
69	10609.5
70	7969.5
71	5926.0
72	4294.0
73	3062.0
74	2096.0
75	1421.5
76	936.0
77	592.0
78	357.5
79	207.5
80	142.0
81	95.0
82	61.5
83	44.0
84	32.0
85	26.5
86	19.5
87	12.0
88	6.5
89	3.5
90	2.5
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003295253574060681
3	0.0
4	0.0
5	0.0036391061209191866
6	1.002903261670642E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.4327957197233787E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	7.183142256960537E-6
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	4.577235636315667E-4
50-51	0.0015493627735437767
52-53	0.00403243999063561
54-55	0.00582852435396437
56-57	0.006665055430658622
58-59	0.00826928587488617
60-61	0.007791228892914922
62-63	0.005679138159233569
64-65	0.003320703801721253
66-67	0.0011848634336813924
68-69	4.6009848367683587E-4
70-71	2.604786278506842E-4
72-73	5.005974753966598E-4
74-75	0.0
76-77	3.8351793329856104E-4
78-79	3.111210819344937E-4
80-81	1.1877140483411495E-4
82-83	3.423355603973215E-5
84-85	1.208109451954954E-4
86-87	1.4794104357960237E-4
88-89	1.755972501470627E-5
90-91	1.7726742093363382E-5
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	61.0
20-21	210.0
22-23	426.0
24-25	556.0
26-27	1290.0
28-29	3121.0
30-31	10348.0
32-33	13316.0
34-35	19111.0
36-37	32243.0
38-39	38667.0
40-41	37525.0
42-43	43466.0
44-45	51293.0
46-47	52230.0
48-49	48831.0
50-51	44974.0
52-53	42508.0
54-55	46101.0
56-57	47968.0
58-59	48133.0
60-61	47069.0
62-63	44859.0
64-65	47433.0
66-67	50877.0
68-69	51770.0
70-71	50730.0
72-73	47208.0
74-75	47845.0
76-77	50278.0
78-79	52385.0
80-81	52395.0
82-83	48288.0
84-85	48459.0
86-87	49898.0
88-89	52943.0
90-91	54177.0
92-93	53784.0
94-95	66011.0
96-97	218944.0
98-99	499858.0
100-101	4762147.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	86.05851717553328
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.0453382618424	77.49168289383469
2	7.159612507319083	12.322912718625645
3	1.624538357508632	4.194160864259077
4	0.5773610934350084	1.9874735830344537
5	0.23156192959282773	0.9963938147531998
6	0.12728350758605267	0.6572297954253861
7	0.07440845191730408	0.44824367261311093
8	0.03920998066955214	0.26994822359223847
9	0.02600427103626616	0.20141001050505603
>10	0.09247765414360516	1.206492261513962
>50	0.0015487305463339378	0.09117919777238565
>100	5.790049049433093E-4	0.0871582908680692
>500	7.624949803237834E-5	0.04571467320260198
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.4327189452437743E-5	0.0
4	0.0	1.4327189452437743E-5	0.0	4.2981568357313227E-5	0.0
5	0.0	1.4327189452437743E-5	0.0	4.2981568357313227E-5	0.0
6	0.0	1.4327189452437743E-5	0.0	5.730875780975097E-5	0.0
7	0.0	1.4327189452437743E-5	0.0	5.730875780975097E-5	0.0
8	0.0	1.4327189452437743E-5	0.0	7.16359472621887E-5	0.0
9	0.0	1.1461751561950195E-4	0.0	7.16359472621887E-5	0.0
10-11	0.0	1.1461751561950195E-4	0.0	7.879954198840758E-5	0.0
12-13	0.0	1.289447050719397E-4	0.0	8.596313671462645E-5	0.0
14-15	0.0	1.5043548925059629E-4	0.0	8.596313671462645E-5	0.0
16-17	0.0	1.5759908397681516E-4	0.0	1.002903261670642E-4	0.0
18-19	0.0	1.719262734292529E-4	0.0	1.3610829979815854E-4	0.0
20-21	0.0	1.8625346288169065E-4	0.0	1.432718945243774E-4	0.0
22-23	0.0	1.8625346288169065E-4	0.0	1.5759908397681516E-4	7.163594726218872E-6
24-25	0.0	2.2207143651278502E-4	0.0	1.6476267870303403E-4	1.4327189452437743E-5
26-27	0.0	2.292350312390039E-4	0.0	2.3639862596522277E-4	1.4327189452437743E-5
28-29	0.0	2.292350312390039E-4	0.0	4.011613046682568E-4	1.4327189452437743E-5
30-31	0.0	2.578894101438794E-4	0.0	9.455945038608911E-4	1.4327189452437743E-5
32-33	0.0	2.578894101438794E-4	0.0	0.0023066775018424765	2.8654378904875487E-5
34-35	0.0	3.2236176267984925E-4	0.0	0.004133394157028288	2.8654378904875487E-5
36-37	0.0	3.939977099420379E-4	0.0	0.007328357404921906	2.8654378904875487E-5
38-39	0.0	4.011613046682568E-4	0.0	0.012013348355869046	2.8654378904875487E-5
40-41	0.0	4.7279725193044553E-4	0.0	0.017056519043127133	2.8654378904875487E-5
42-43	0.0	4.7279725193044553E-4	0.0	0.02183463672551512	2.8654378904875487E-5
44-45	0.0	5.157788202877588E-4	0.0	0.027021079307297583	2.8654378904875487E-5
46-47	0.0	5.58760388645072E-4	0.0	0.03329638828746531	2.8654378904875487E-5
48-49	0.0	5.58760388645072E-4	0.0	0.039285153478584287	2.8654378904875487E-5
50-51	0.0	6.375599306334796E-4	0.0	0.04505184723319048	2.8654378904875487E-5
52-53	0.0	6.518871200859172E-4	0.0	0.05082570458252289	2.8654378904875487E-5
54-55	0.0	7.020322831694494E-4	0.0	0.056449126442604705	2.8654378904875487E-5
56-57	0.0	7.306866620743249E-4	0.0	0.06249520039153344	2.8654378904875487E-5
58-59	0.0	7.450138515267626E-4	0.0	0.0684409840142951	2.8654378904875487E-5
60-61	0.0	7.665046357054192E-4	0.0	0.07412171463218667	2.8654378904875487E-5
62-63	4.2981568357313227E-5	7.879954198840759E-4	0.0	0.07948008348739838	2.8654378904875487E-5
64-65	4.2981568357313227E-5	8.739585565987023E-4	0.0	0.08451609057993024	2.8654378904875487E-5
66-67	4.2981568357313227E-5	8.811221513249212E-4	0.0	0.08950911610410481	2.8654378904875487E-5
68-69	4.2981568357313227E-5	8.882857460511401E-4	0.0	0.09453795960191044	2.8654378904875487E-5
70-71	5.01451630835321E-5	9.026129355035778E-4	0.0	0.09880746205873689	2.8654378904875487E-5
72-73	5.730875780975097E-5	9.169401249560156E-4	0.0	0.10254685850582314	2.8654378904875487E-5
74-75	5.730875780975097E-5	9.312673144084533E-4	0.0	0.10543378718048935	2.8654378904875487E-5
76-77	5.730875780975097E-5	9.670852880395476E-4	0.0	0.10796253611884461	4.2981568357313227E-5
78-79	5.730875780975097E-5	9.957396669444231E-4	0.0	0.10960299931114872	6.447235253596983E-5
80-81	5.730875780975097E-5	0.00101723045112308	0.0	0.11024772283650844	7.16359472621887E-5
82-83	5.730875780975097E-5	0.0010315576405755175	0.0	0.11084946479351082	7.16359472621887E-5
84-85	5.730875780975097E-5	0.0010315576405755175	0.0	0.11100706387748763	7.16359472621887E-5
86-87	5.730875780975097E-5	0.0010315576405755175	0.0	0.11108586341947604	7.16359472621887E-5
88	5.730875780975097E-5	0.0011031935878377062	0.0	0.1111073542036547	7.16359472621887E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8840	0.0	49.090443	1
GTATCAA	15945	0.0	36.387527	1
TCAACGC	19105	0.0	31.658686	4
ATCAACG	19290	0.0	31.26535	3
CAACGCA	19845	0.0	30.521767	5
TATCAAC	20425	0.0	29.846762	2
AACGCAG	20425	0.0	29.69678	6
ACGCAGA	23460	0.0	25.854933	7
CGCAGAG	23795	0.0	25.47275	8
GTGGTAT	4545	0.0	23.70357	1
TGGTATC	4535	0.0	22.80179	2
GCAGAGT	28140	0.0	21.585712	9
GAGTACT	16130	0.0	20.867414	12-13
TACATGG	20465	0.0	19.682772	2
CAGAGTA	27260	0.0	19.306772	10-11
GTACTTT	17660	0.0	18.92527	14-15
GTACATG	20980	0.0	18.910898	1
AGAGTAC	25170	0.0	18.898853	10-11
ACATGGG	20970	0.0	18.44471	3
AGTACTT	17875	0.0	17.341774	12-13
>>END_MODULE