FastQCFastQC Report
Fri 27 May 2016
SRR2139778_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139778_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12445390
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147300.047.703751
GTATCAA278500.038.7499771
TCAACGC318500.033.273994
ATCAACG321250.032.935583
CAACGCA328450.032.239795
TATCAAC335550.031.8411392
AACGCAG339450.031.2313216
ACGCAGA392200.027.041757
CGCAGAG399500.026.5583938
TGGTATC75900.023.6975612
GTGGTAT79700.023.53951
GCAGAGT470200.022.565039
GAGTACT256850.020.52859512-13
GTACATG368200.020.4747891
TACATGG363250.020.3155022
ACATGGG367000.019.4504763
CAGAGTA457350.019.19611710-11
AGAGTAC418100.018.718810-11
GTACTTT288500.018.24685514-15
CATGGGG273750.017.8084324
Produced by FastQC (version 0.11.5)