##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2139778_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12445390
Sequences flagged as poor quality	0
Sequence length	19-100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.31198459831311	32.0	27.0	33.0	18.0	33.0
2	30.698754960672186	32.0	31.0	33.0	25.0	33.0
3	30.47357567741951	32.0	31.0	33.0	25.0	33.0
4	31.29337128045003	33.0	31.0	33.0	28.0	33.0
5	31.68390914226071	33.0	32.0	33.0	30.0	33.0
6	35.14032947139463	37.0	35.0	38.0	29.0	38.0
7	36.50691726012604	38.0	37.0	38.0	34.0	38.0
8	36.4859428270227	38.0	37.0	38.0	34.0	38.0
9	36.88033536916079	38.0	38.0	38.0	35.0	38.0
10-11	36.993601204944156	38.0	38.0	38.0	35.5	38.0
12-13	37.06082726214285	38.0	38.0	38.0	36.0	38.0
14-15	37.10899091952924	38.0	38.0	38.0	36.0	38.0
16-17	37.078649604391664	38.0	38.0	38.0	36.0	38.0
18-19	37.12879841451333	38.0	38.0	38.0	36.0	38.0
20-21	37.166209402776616	38.0	38.0	38.0	36.0	38.0
22-23	37.18250110594782	38.0	38.0	38.0	36.0	38.0
24-25	37.18749742737583	38.0	38.0	38.0	36.0	38.0
26-27	37.163625290455634	38.0	38.0	38.0	36.0	38.0
28-29	37.143222831878845	38.0	38.0	38.0	36.0	38.0
30-31	37.14360680970289	38.0	38.0	38.0	36.0	38.0
32-33	37.13447609681204	38.0	38.0	38.0	36.0	38.0
34-35	37.11960338879572	38.0	38.0	38.0	36.0	38.0
36-37	37.107310723935676	38.0	38.0	38.0	36.0	38.0
38-39	37.089453108832664	38.0	38.0	38.0	36.0	38.0
40-41	37.06585836457847	38.0	38.0	38.0	36.0	38.0
42-43	37.040588192601334	38.0	38.0	38.0	36.0	38.0
44-45	36.99895471743285	38.0	38.0	38.0	36.0	38.0
46-47	36.97810315319288	38.0	38.0	38.0	36.0	38.0
48-49	36.95026486737969	38.0	38.0	38.0	36.0	38.0
50-51	36.91700104445158	38.0	38.0	38.0	36.0	38.0
52-53	36.89335352664833	38.0	38.0	38.0	36.0	38.0
54-55	36.8634337372604	38.0	38.0	38.0	35.0	38.0
56-57	36.864599180767435	38.0	38.0	38.0	35.0	38.0
58-59	36.85047325138892	38.0	38.0	38.0	35.0	38.0
60-61	36.85784780909178	38.0	38.0	38.0	35.0	38.0
62-63	36.84440936784219	38.0	38.0	38.0	35.0	38.0
64-65	36.833373448944556	38.0	38.0	38.0	35.0	38.0
66-67	36.81941659470664	38.0	38.0	38.0	35.0	38.0
68-69	36.82575016882714	38.0	38.0	38.0	35.0	38.0
70-71	36.80171569178996	38.0	38.0	38.0	35.0	38.0
72-73	36.78197072300023	38.0	38.0	38.0	35.0	38.0
74-75	36.789799460150135	38.0	38.0	38.0	35.0	38.0
76-77	36.76733678927866	38.0	38.0	38.0	35.0	38.0
78-79	36.73917585189881	38.0	38.0	38.0	35.0	38.0
80-81	36.72949415423821	38.0	38.0	38.0	35.0	38.0
82-83	36.666942375841884	38.0	38.0	38.0	34.0	38.0
84-85	36.66412933300432	38.0	38.0	38.0	34.0	38.0
86-87	36.64851604510574	38.0	38.0	38.0	34.0	38.0
88-89	36.62097204884281	38.0	38.0	38.0	34.0	38.0
90-91	36.595017255653495	38.0	38.0	38.0	34.0	38.0
92-93	36.59508129541075	38.0	38.0	38.0	34.0	38.0
94-95	36.5342302011654	38.0	38.0	38.0	34.0	38.0
96-97	36.500232733996505	38.0	38.0	38.0	34.0	38.0
98-99	36.52681562521087	38.0	38.0	38.0	34.0	38.0
100	34.875461306230655	38.0	34.0	38.0	28.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	6.0
18	29.0
19	43.0
20	109.0
21	369.0
22	1340.0
23	3969.0
24	9167.0
25	18194.0
26	30754.0
27	48887.0
28	71410.0
29	101956.0
30	140153.0
31	185758.0
32	243303.0
33	329247.0
34	480383.0
35	818595.0
36	2103367.0
37	7858351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.70583565480873	18.90513676148357	14.618601747313665	25.77042583639404
2	14.041263835642129	19.484375217206733	43.21104455215979	23.263316394991346
3	18.15483484245974	24.486512676581448	29.967811374332182	27.390841106626628
4	12.322000355151587	17.225759899850466	39.129364367046755	31.322875377951192
5	13.739991895402968	36.46859741856612	35.41766758761438	14.373743098416533
6	30.541422231721516	37.37797269185247	18.45499973082359	13.62560534560242
7	27.34749172183435	32.29568539033329	22.237326431714877	18.11949645611749
8	24.52987009647749	36.92989130915142	20.490414522967942	18.049824071403144
9	25.324750771169086	17.40928166975884	21.094935554450284	36.171032004621786
10-11	24.549130240193357	26.756521892845463	28.24154566469994	20.45280220226124
12-13	25.235569154522274	24.006981701658205	28.607026376835115	22.150422766984402
14-15	22.259105580459913	25.549484588269227	26.27631998675815	25.915089844512707
16-17	21.97366655444305	28.786277489094353	26.93040957334403	22.30964638311857
18-19	21.98205118521798	27.875381968745057	28.716902403219187	21.425664442817784
20-21	22.760842475270998	26.941990061995913	28.69158451588238	21.60558294685071
22-23	22.495372494366055	26.777575467886212	28.590288881463255	22.136763156284477
24-25	22.263330192377264	26.916391692382806	28.665412159276848	22.154865955963075
26-27	22.177081300752896	27.087717947539254	28.617425259171082	22.11777549253677
28-29	22.138461423449662	27.109829619120678	28.66138989711328	22.090319060316375
30-31	22.429124205303722	26.972915388109747	28.52399246497086	22.07396794161567
32-33	22.153345382639465	27.112167608827004	28.535804637668104	22.198682370865427
34-35	22.322092194029608	27.10778175910799	28.52568429076588	22.04444175609652
36-37	22.30968796443511	27.011683708446988	28.52012437339246	22.158503953725447
38-39	22.14037238494576	27.183029868587987	28.594044203147938	22.082553543318312
40-41	22.411313676781504	27.057695119292006	28.444802439925915	22.08618876400057
42-43	22.278497338337864	27.097760198642938	28.332487936092086	22.291254526927105
44-45	22.324974023898804	27.174456205191717	28.25891927053888	22.241650500370604
46-47	22.394063541170574	27.173918772058375	28.042948376301986	22.389069310469072
48-49	22.26351866983389	27.261886993558466	28.06243712565257	22.412157210955083
50-51	22.2825212686211	27.40814033303328	27.95412382736757	22.355214570978045
52-53	22.52281903919709	27.300058879563487	27.8066053595974	22.37051672164203
54-55	22.430136580599665	27.227413439001584	27.887617415253285	22.45483256514546
56-57	22.384291781127683	27.36560860842655	27.882688527286316	22.36741108315945
58-59	22.37751997487219	27.354612992280863	27.91657510773829	22.351291925108654
60-61	22.435295112025386	27.310251726043038	27.962342820107377	22.29211034182419
62-63	22.40205664550935	27.34300104082402	27.907825456674214	22.347116856992415
64-65	22.39026093246273	27.312287476171925	27.863210710103225	22.434240881262113
66-67	22.466036308209762	27.342113206718565	27.721634919901266	22.470215565170406
68-69	22.394412214372323	27.319373258876446	27.85131302788651	22.434901498864722
70-71	22.41593598584789	27.128459335685395	27.880754582859772	22.574850095606937
72-73	22.441402325642137	27.20505454886062	27.758529671823517	22.595013453673726
74-75	22.318309111530702	27.34007521303991	27.74727059978383	22.59434507564556
76-77	22.468882777842488	27.27031173573246	27.67555346302453	22.58525202340052
78-79	22.434100171026724	27.290620871548	27.620437372090183	22.654841585335095
80-81	22.476826610865754	27.33641965631966	27.588946171723993	22.597807561090598
82-83	22.497934050163483	27.308075856882606	27.65783366533816	22.536156427615747
84-85	22.560156594278187	27.299265876166203	27.618680120787754	22.521897408767856
86-87	22.394430107057143	27.352695741247423	27.696163775078496	22.556710376616934
88-89	22.50408024100602	27.327673276962283	27.608638739994234	22.559607742037464
90-91	22.51559878472641	27.370685408073303	27.628765480028882	22.4849503271714
92-93	22.541928071861435	27.34612132969132	27.635113814023043	22.476836784424204
94-95	22.428291924636245	27.390299631850823	27.624045303034272	22.557363140478657
96-97	22.564233711111246	27.49637566984501	27.66188308756754	22.277507531476207
98-99	23.601484309333394	29.37602150605132	27.905459966633185	19.117034217982106
100	22.500325599735543	30.319080551876876	21.761121342724184	25.4194725056634
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1470.0
1	1414.0
2	1929.0
3	2816.5
4	3671.5
5	5114.5
6	6478.5
7	8213.0
8	10609.5
9	12359.0
10	12901.5
11	13361.5
12	15032.0
13	18342.0
14	24611.5
15	32327.5
16	38531.0
17	42273.0
18	42236.5
19	38981.0
20	35066.5
21	32033.5
22	31680.0
23	35745.5
24	44610.0
25	58461.5
26	77122.0
27	99323.0
28	123017.5
29	150232.0
30	179199.5
31	208018.5
32	238833.0
33	270643.0
34	303539.0
35	335044.0
36	367569.0
37	398053.5
38	421916.5
39	443180.5
40	465894.0
41	483762.0
42	495811.5
43	508494.5
44	518098.5
45	526801.5
46	532344.5
47	531404.5
48	528085.0
49	520886.5
50	510789.5
51	495786.0
52	476759.0
53	455956.5
54	430752.5
55	401596.0
56	370107.5
57	337810.5
58	301828.5
59	262246.5
60	221809.5
61	181781.0
62	143566.5
63	110802.0
64	84393.0
65	63192.0
66	46925.5
67	35905.0
68	27755.0
69	20621.5
70	15079.0
71	10710.5
72	7366.5
73	4990.5
74	3318.0
75	2174.5
76	1406.5
77	831.0
78	506.5
79	326.5
80	208.0
81	131.5
82	88.5
83	59.0
84	45.0
85	38.5
86	31.0
87	22.5
88	14.5
89	9.5
90	6.0
91	3.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.003607761588829277
3	0.0
4	0.0
5	0.004829097360548765
6	1.6070207522624844E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	1.2053148599813852E-5
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.6115517966647083E-5
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.0
46-47	0.0
48-49	3.9210671475159854E-4
50-51	0.0015716764533260305
52-53	0.00402096514114101
54-55	0.005996990389693655
56-57	0.006486143769153715
58-59	0.008117651064234994
60-61	0.0077325618709712
62-63	0.005583469941638514
64-65	0.0033333324372762266
66-67	0.00123343035415174
68-69	4.330142261582253E-4
70-71	2.4835298280670697E-4
72-73	5.149571866450725E-4
74-75	0.0
76-77	3.0676796679807817E-4
78-79	2.3340175339018427E-4
80-81	6.251247544642203E-5
82-83	4.8536349964556325E-6
84-85	1.0774464597264226E-4
86-87	1.5319692580223175E-4
88-89	1.9957764381128436E-5
90-91	5.04003552217036E-6
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
18-19	85.0
20-21	292.0
22-23	648.0
24-25	878.0
26-27	2154.0
28-29	5813.0
30-31	19427.0
32-33	25501.0
34-35	36425.0
36-37	59943.0
38-39	71319.0
40-41	70034.0
42-43	79728.0
44-45	95100.0
46-47	96169.0
48-49	89986.0
50-51	83342.0
52-53	81322.0
54-55	87598.0
56-57	91140.0
58-59	90823.0
60-61	88663.0
62-63	86707.0
64-65	91510.0
66-67	96672.0
68-69	98240.0
70-71	95562.0
72-73	90358.0
74-75	91736.0
76-77	96456.0
78-79	99057.0
80-81	97395.0
82-83	93264.0
84-85	91003.0
86-87	95237.0
88-89	99694.0
90-91	101815.0
92-93	101436.0
94-95	123115.0
96-97	389919.0
98-99	883687.0
100-101	8346137.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.5035995876509
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.79658875217574	74.14834791910988
2	7.677165331868355	12.821418796810601
3	1.946231626038787	4.875520392166968
4	0.7189338499192699	2.4013425733466804
5	0.32556980731282337	1.359312541383933
6	0.17715649479178475	0.8875923003266972
7	0.09869809441288807	0.576915230914258
8	0.06901295001303706	0.46102637954009706
9	0.04444493102367101	0.33401805515112487
>10	0.1424842571228896	1.7987274733215213
>50	0.002760018290564764	0.15809119879781972
>100	8.86422631713486E-4	0.1292711066465681
>500	5.060201308886463E-5	0.026802159943083475
>1k	1.6862385326115727E-5	0.021613872540797344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	8.035103761312422E-6	0.0	0.0	0.0
3	0.0	8.035103761312422E-6	0.0	1.6070207522624844E-5	0.0
4	0.0	5.6245726329186955E-5	0.0	2.4105311283937266E-5	0.0
5	0.0	5.6245726329186955E-5	0.0	4.017551880656211E-5	0.0
6	0.0	5.6245726329186955E-5	0.0	4.017551880656211E-5	8.035103761312422E-6
7	0.0	5.6245726329186955E-5	0.0	4.017551880656211E-5	8.035103761312422E-6
8	0.0	7.23159338518118E-5	0.0	4.821062256787453E-5	8.035103761312422E-6
9	0.0	9.642124513574907E-5	0.0	5.6245726329186955E-5	1.6070207522624844E-5
10-11	0.0	9.642124513574907E-5	0.0	7.633348573246802E-5	1.6070207522624844E-5
12-13	0.0	9.642124513574907E-5	0.0	8.436858949378043E-5	1.6070207522624844E-5
14-15	0.0	1.7677228274887329E-4	0.0	9.642124513574907E-5	4.017551880656211E-5
16-17	0.0	1.7677228274887329E-4	0.0	9.642124513574907E-5	4.017551880656211E-5
18-19	8.035103761312422E-6	1.7677228274887329E-4	0.0	9.642124513574907E-5	4.821062256787453E-5
20-21	1.6070207522624844E-5	1.848073865101857E-4	0.0	9.642124513574907E-5	4.821062256787453E-5
22-23	1.6070207522624844E-5	2.0087759403281054E-4	0.0	1.2052655641968633E-4	5.6245726329186955E-5
24-25	1.6070207522624844E-5	2.0489514591346677E-4	0.0	1.446318677036236E-4	7.23159338518118E-5
26-27	2.4105311283937266E-5	2.0891269779412297E-4	0.0	1.848073865101857E-4	8.838614137443664E-5
28-29	2.4105311283937266E-5	2.1293024967477918E-4	0.0	3.655972211397152E-4	9.642124513574907E-5
30-31	2.4105311283937266E-5	2.892637354072472E-4	0.0	0.001104826767180458	1.0043879701640527E-4
32-33	2.4105311283937266E-5	2.892637354072472E-4	0.0	0.00267167200063638	1.2856166018099875E-4
34-35	2.4105311283937266E-5	3.3345680609446547E-4	0.0	0.0047889218417422035	1.3659676394231116E-4
36-37	2.4105311283937266E-5	3.696147730203714E-4	0.0	0.00813152500644817	1.406143158229674E-4
38-39	2.8122863164593477E-5	3.776498767816838E-4	0.0	0.013322202036255995	1.446318677036236E-4
40-41	3.214041504524969E-5	4.097902918269335E-4	0.0	0.019059266121833063	1.486494195842798E-4
42-43	3.214041504524969E-5	4.178253955882459E-4	0.0	0.024575364853974043	1.566845233455922E-4
44-45	3.214041504524969E-5	4.5800091439480805E-4	0.0	0.031039605829949884	1.6471962710690465E-4
46-47	3.61579669259059E-5	4.901413294400577E-4	0.0	0.03778909298945232	1.7275473086821708E-4
48-49	4.017551880656211E-5	4.94158881320714E-4	0.0	0.045044791685917435	1.7677228274887329E-4
50-51	4.017551880656211E-5	5.986152302177754E-4	0.0	0.05164161187395494	1.848073865101857E-4
52-53	4.017551880656211E-5	6.10667885859744E-4	0.0	0.05835092351465081	1.888249383908419E-4
54-55	4.017551880656211E-5	6.187029896210564E-4	0.0	0.06478704162746206	1.9284249027149813E-4
56-57	4.017551880656211E-5	6.508434046663061E-4	0.0	0.07097808907555328	2.0087759403281054E-4
58-59	4.017551880656211E-5	6.548609565469624E-4	0.0	0.07704860996722482	2.0891269779412297E-4
60-61	4.017551880656211E-5	6.789662678308996E-4	0.0	0.08317537658522553	2.1694780155543538E-4
62-63	4.419307068721832E-5	6.990540272341807E-4	0.0	0.08843836954888518	2.1694780155543538E-4
64-65	4.821062256787453E-5	8.035103761312422E-4	0.0	0.09377769599827727	2.1694780155543538E-4
66-67	4.821062256787453E-5	8.115454798925546E-4	0.0	0.09954288294701893	2.1694780155543538E-4
68-69	4.821062256787453E-5	8.276156874151794E-4	0.0	0.1053361927589252	2.209653534360916E-4
70-71	4.821062256787453E-5	8.356507911764919E-4	0.0	0.11047062406240382	2.2498290531674782E-4
72-73	4.821062256787453E-5	8.356507911764919E-4	0.0	0.11434756162723708	2.571233203619975E-4
74-75	4.821062256787453E-5	8.517209986991167E-4	0.0	0.11737277819337119	2.571233203619975E-4
76-77	4.821062256787453E-5	8.95914069386335E-4	0.0	0.12036585434446007	2.6114087224265374E-4
78-79	4.821062256787453E-5	9.280544844315847E-4	0.0	0.12237864783666884	2.651584241233099E-4
80-81	4.821062256787453E-5	9.320720363122409E-4	0.0	0.12300136837817055	2.731935278846223E-4
82-83	4.821062256787453E-5	9.441246919542096E-4	0.0	0.12356382564146243	2.892637354072472E-4
84-85	4.821062256787453E-5	9.52159795715522E-4	0.0	0.12379684365054049	2.892637354072472E-4
86-87	4.821062256787453E-5	9.802826588801155E-4	0.0	0.12387719468815361	3.0131639104921584E-4
88	4.821062256787453E-5	0.0010686688002545521	0.0	0.12388522979191492	3.05333942929872E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14730	0.0	47.70375	1
GTATCAA	27850	0.0	38.749977	1
TCAACGC	31850	0.0	33.27399	4
ATCAACG	32125	0.0	32.93558	3
CAACGCA	32845	0.0	32.23979	5
TATCAAC	33555	0.0	31.841139	2
AACGCAG	33945	0.0	31.231321	6
ACGCAGA	39220	0.0	27.04175	7
CGCAGAG	39950	0.0	26.558393	8
TGGTATC	7590	0.0	23.697561	2
GTGGTAT	7970	0.0	23.5395	1
GCAGAGT	47020	0.0	22.56503	9
GAGTACT	25685	0.0	20.528595	12-13
GTACATG	36820	0.0	20.474789	1
TACATGG	36325	0.0	20.315502	2
ACATGGG	36700	0.0	19.450476	3
CAGAGTA	45735	0.0	19.196117	10-11
AGAGTAC	41810	0.0	18.7188	10-11
GTACTTT	28850	0.0	18.246855	14-15
CATGGGG	27375	0.0	17.808432	4
>>END_MODULE