FastQCFastQC Report
Fri 27 May 2016
SRR2139777_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139777_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10819004
Sequences flagged as poor quality0
Sequence length19-100
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA128900.047.270711
GTATCAA234450.034.6769071
ATCAACG294750.030.1962623
TCAACGC295750.030.108724
CAACGCA305600.029.1523575
TATCAAC311450.028.8412862
AACGCAG313100.028.522416
TGGTATC67650.024.8884352
ACGCAGA360700.024.7821927
CGCAGAG370900.024.0774468
GTGGTAT67550.023.0128541
GCAGAGT435550.020.4936719
GAGTACT245800.019.58476612-13
TACATGG324400.019.2077432
ACATGGG325650.018.4851363
CAGAGTA424950.018.3906610-11
AGAGTAC390500.018.38116610-11
GTACATG322100.017.887681
GTACTTT272800.017.53598814-15
CATGGGG230700.016.3688644
Produced by FastQC (version 0.11.5)