FastQCFastQC Report
Fri 27 May 2016
SRR2139776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2139776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9295249
Sequences flagged as poor quality0
Sequence length19-101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105000.048.9753071
GTATCAA187900.034.660141
TCAACGC226600.028.0289884
ATCAACG230650.027.536383
CAACGCA233800.027.1139625
TATCAAC240150.026.6323872
AACGCAG241400.026.3438056
GTACATG311250.022.5944521
TACATGG307700.022.5931822
ACGCAGA286050.022.2598177
CGCAGAG290850.021.8647448
ACATGGG313000.021.5404973
CATGGGG236200.019.2801154
GTGGTAT51350.019.1498411
ACCGAAC15800.018.619688
GCAGAGT343900.018.4216759
CTTATAC151450.018.1746981
GAGTACT180200.017.7455412-13
TATACAC170250.017.5165543
GGACCGA21050.017.2305536
Produced by FastQC (version 0.11.5)